Mycoplasma phage MAV1

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified dsDNA phages

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|O80246|O80246_9VIRU HtpH OS=Mycoplasma phage MAV1 OX=75590 GN=htpH PE=4 SV=1
MM1 pKa = 7.47NDD3 pKa = 3.09KK4 pKa = 11.41NEE6 pKa = 4.21LLKK9 pKa = 11.19EE10 pKa = 4.0MGKK13 pKa = 7.97MASYY17 pKa = 10.13FSQLLARR24 pKa = 11.84SSKK27 pKa = 10.59SLQSEE32 pKa = 4.22LADD35 pKa = 3.94GADD38 pKa = 3.3VCRR41 pKa = 11.84LKK43 pKa = 10.31EE44 pKa = 4.25TIKK47 pKa = 10.98SLNEE51 pKa = 3.56DD52 pKa = 5.45ANWLWNKK59 pKa = 10.38LEE61 pKa = 4.62EE62 pKa = 3.97NWIMIRR68 pKa = 11.84LRR70 pKa = 11.84DD71 pKa = 3.69YY72 pKa = 11.36VDD74 pKa = 3.59YY75 pKa = 10.69EE76 pKa = 4.54GKK78 pKa = 8.16ITLYY82 pKa = 10.11KK83 pKa = 9.56YY84 pKa = 10.8KK85 pKa = 10.87VMNLTPNKK93 pKa = 9.69SLKK96 pKa = 9.83IHH98 pKa = 6.93LEE100 pKa = 4.01LNDD103 pKa = 4.94LIILEE108 pKa = 4.43PSSEE112 pKa = 3.51ISGYY116 pKa = 10.42IKK118 pKa = 10.58DD119 pKa = 3.48SARR122 pKa = 11.84NIIEE126 pKa = 3.87KK127 pKa = 10.38SGEE130 pKa = 4.05DD131 pKa = 3.54FSTFTTIDD139 pKa = 3.25SFNFEE144 pKa = 4.01TNEE147 pKa = 3.89PVFEE151 pKa = 4.32PLEE154 pKa = 4.18EE155 pKa = 4.06QDD157 pKa = 3.25QSFYY161 pKa = 11.1LYY163 pKa = 10.88SLNPKK168 pKa = 8.6ATINFKK174 pKa = 10.5RR175 pKa = 11.84DD176 pKa = 2.65NFYY179 pKa = 11.29YY180 pKa = 9.85IDD182 pKa = 4.01SNSLVEE188 pKa = 4.24YY189 pKa = 10.48QWTDD193 pKa = 3.5DD194 pKa = 4.26DD195 pKa = 4.74DD196 pKa = 4.5EE197 pKa = 6.57NYY199 pKa = 10.83LFTINFSNVKK209 pKa = 8.38QLKK212 pKa = 10.16NYY214 pKa = 8.5DD215 pKa = 3.43ASTIQYY221 pKa = 9.31KK222 pKa = 9.3WDD224 pKa = 3.63SEE226 pKa = 3.99ISEE229 pKa = 4.33EE230 pKa = 4.2STNTVEE236 pKa = 4.96LTFEE240 pKa = 4.48PSTLISDD247 pKa = 4.15LVFVGRR253 pKa = 11.84KK254 pKa = 8.84KK255 pKa = 11.14DD256 pKa = 3.4NDD258 pKa = 3.43KK259 pKa = 10.78TINNYY264 pKa = 9.43VSSARR269 pKa = 11.84LVNDD273 pKa = 3.44VYY275 pKa = 10.64FYY277 pKa = 10.48PHH279 pKa = 7.35LMNPIHH285 pKa = 5.5VQKK288 pKa = 10.95DD289 pKa = 3.58YY290 pKa = 11.19IQWNLPMLEE299 pKa = 3.34IVAYY303 pKa = 9.1VNRR306 pKa = 11.84LLKK309 pKa = 10.78DD310 pKa = 3.52AVDD313 pKa = 3.67VEE315 pKa = 4.68YY316 pKa = 10.51TSLRR320 pKa = 11.84TNILNIFSEE329 pKa = 4.32KK330 pKa = 10.57VINKK334 pKa = 7.25EE335 pKa = 3.83TLADD339 pKa = 4.67HH340 pKa = 6.06ITLFIDD346 pKa = 3.72TLFLCMDD353 pKa = 3.58VTKK356 pKa = 10.4KK357 pKa = 10.79VSSGYY362 pKa = 11.16AEE364 pKa = 4.34MGTAEE369 pKa = 4.23TNTFKK374 pKa = 10.57EE375 pKa = 4.61TVYY378 pKa = 10.86SGNNPKK384 pKa = 10.12EE385 pKa = 3.86FCDD388 pKa = 4.58YY389 pKa = 10.32KK390 pKa = 11.27LNWILQEE397 pKa = 4.06TEE399 pKa = 3.78KK400 pKa = 10.93FGNQSLIRR408 pKa = 11.84TFEE411 pKa = 4.74LIILMCSNQIASSYY425 pKa = 9.46CFKK428 pKa = 11.19GGLTTEE434 pKa = 4.24HH435 pKa = 6.59EE436 pKa = 4.83FYY438 pKa = 11.19LPFFIQSSYY447 pKa = 11.26KK448 pKa = 10.15NNKK451 pKa = 8.86NEE453 pKa = 3.76YY454 pKa = 9.17KK455 pKa = 10.01RR456 pKa = 11.84YY457 pKa = 9.6KK458 pKa = 10.37GSFVFKK464 pKa = 10.21SHH466 pKa = 6.06YY467 pKa = 9.73FALNEE472 pKa = 4.02TGQWQPLKK480 pKa = 10.81FDD482 pKa = 3.98INKK485 pKa = 9.75HH486 pKa = 4.2NARR489 pKa = 11.84FSKK492 pKa = 10.67NKK494 pKa = 9.06TLTFPLLPKK503 pKa = 10.43VLEE506 pKa = 4.21EE507 pKa = 4.2VNISNTPVDD516 pKa = 4.66FNTEE520 pKa = 3.2NSQNFKK526 pKa = 10.63YY527 pKa = 10.83LMWNVSEE534 pKa = 4.17QNHH537 pKa = 4.84VEE539 pKa = 4.21KK540 pKa = 10.92YY541 pKa = 10.87LKK543 pKa = 9.53TEE545 pKa = 4.2YY546 pKa = 10.53EE547 pKa = 4.3WIDD550 pKa = 3.27NGPTKK555 pKa = 10.31EE556 pKa = 4.41YY557 pKa = 10.37ILEE560 pKa = 4.19TEE562 pKa = 4.07PMEE565 pKa = 4.75IRR567 pKa = 11.84DD568 pKa = 3.63WNNAFDD574 pKa = 3.31IWLKK578 pKa = 9.2YY579 pKa = 10.38EE580 pKa = 3.91RR581 pKa = 11.84DD582 pKa = 3.45KK583 pKa = 10.61ITNYY587 pKa = 5.98EE588 pKa = 3.95TRR590 pKa = 11.84PILLRR595 pKa = 11.84AFKK598 pKa = 10.58DD599 pKa = 3.23NQNRR603 pKa = 11.84HH604 pKa = 6.14EE605 pKa = 4.34INKK608 pKa = 8.99EE609 pKa = 3.64QLLSILSKK617 pKa = 10.6PKK619 pKa = 10.64EE620 pKa = 3.96EE621 pKa = 4.67LKK623 pKa = 10.54YY624 pKa = 10.35LYY626 pKa = 10.53YY627 pKa = 10.59RR628 pKa = 11.84AFPTFFVSKK637 pKa = 9.78HH638 pKa = 4.58YY639 pKa = 10.57EE640 pKa = 3.86VLNVDD645 pKa = 4.04EE646 pKa = 4.77GAKK649 pKa = 8.82NTTAEE654 pKa = 4.23LFAYY658 pKa = 10.1DD659 pKa = 4.35PSTTNTPLFTYY670 pKa = 9.55FKK672 pKa = 10.22FRR674 pKa = 11.84SKK676 pKa = 10.93FKK678 pKa = 9.63ITSKK682 pKa = 8.76TQFRR686 pKa = 11.84DD687 pKa = 3.52DD688 pKa = 3.7YY689 pKa = 11.35QYY691 pKa = 7.82TTLIINPSEE700 pKa = 3.69FRR702 pKa = 11.84KK703 pKa = 10.45VINLPSDD710 pKa = 4.4PIKK713 pKa = 11.21VKK715 pKa = 11.0FEE717 pKa = 4.22LEE719 pKa = 3.49KK720 pKa = 11.29DD721 pKa = 4.35FILEE725 pKa = 4.35NITLKK730 pKa = 10.92SIFANQIGISLNGKK744 pKa = 6.08EE745 pKa = 4.19TKK747 pKa = 9.22TYY749 pKa = 9.68KK750 pKa = 10.57LNYY753 pKa = 9.16SSSISNTEE761 pKa = 3.62TKK763 pKa = 10.26IIII766 pKa = 4.07

Molecular weight: 90.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|O80249|O80249_9VIRU HtpB OS=Mycoplasma phage MAV1 OX=75590 GN=htpB PE=4 SV=1
MM1 pKa = 7.61LSYY4 pKa = 10.83GKK6 pKa = 9.98NLISRR11 pKa = 11.84KK12 pKa = 9.76AIPLKK17 pKa = 10.18PKK19 pKa = 10.67SKK21 pKa = 10.41GKK23 pKa = 8.23PWWYY27 pKa = 9.34YY28 pKa = 10.38VVDD31 pKa = 3.96IVLDD35 pKa = 4.44LIIEE39 pKa = 4.33ALSLAVGVLSAGAASGITRR58 pKa = 11.84LGLGLIKK65 pKa = 10.52DD66 pKa = 3.67ITLSSVFDD74 pKa = 4.44RR75 pKa = 11.84KK76 pKa = 10.55INWKK80 pKa = 10.31DD81 pKa = 3.24VFLNAGLNLVFVGIGKK97 pKa = 9.37SLSKK101 pKa = 10.42FSKK104 pKa = 8.95ISKK107 pKa = 8.74SARR110 pKa = 11.84LIKK113 pKa = 10.52KK114 pKa = 8.52FAFYY118 pKa = 10.33AASPQKK124 pKa = 10.15FLNWIINKK132 pKa = 8.22ATYY135 pKa = 9.99GLKK138 pKa = 8.89KK139 pKa = 9.23TLINTVGQVAGKK151 pKa = 10.26KK152 pKa = 7.24ITKK155 pKa = 9.96FLRR158 pKa = 11.84TGLKK162 pKa = 10.22LISKK166 pKa = 7.06TAIITSAFLLAKK178 pKa = 10.35NKK180 pKa = 10.87AEE182 pKa = 3.92FAYY185 pKa = 10.09KK186 pKa = 10.29NIKK189 pKa = 8.51TFAIRR194 pKa = 11.84KK195 pKa = 7.5FKK197 pKa = 11.07NLLTKK202 pKa = 10.23SIRR205 pKa = 11.84RR206 pKa = 11.84QFTKK210 pKa = 10.84NFWGAIKK217 pKa = 10.33GKK219 pKa = 9.51KK220 pKa = 9.49LKK222 pKa = 10.76ASGYY226 pKa = 8.12NQILQKK232 pKa = 10.76HH233 pKa = 4.76NQTWIPFPSSKK244 pKa = 9.72WIEE247 pKa = 4.18GVKK250 pKa = 9.73IASDD254 pKa = 2.92NWIFNDD260 pKa = 3.52DD261 pKa = 3.65NTLISYY267 pKa = 8.59YY268 pKa = 11.03VFFKK272 pKa = 11.05SRR274 pKa = 11.84FAARR278 pKa = 11.84IRR280 pKa = 11.84TTVQKK285 pKa = 10.95DD286 pKa = 3.09PLIFIDD292 pKa = 4.84RR293 pKa = 11.84PIEE296 pKa = 3.94EE297 pKa = 4.66FNDD300 pKa = 3.78FLSSGSKK307 pKa = 10.3GKK309 pKa = 10.56FYY311 pKa = 10.83LDD313 pKa = 3.08EE314 pKa = 4.67LAWGWEE320 pKa = 3.78LGKK323 pKa = 10.54AIRR326 pKa = 11.84NQSKK330 pKa = 10.21KK331 pKa = 9.81PRR333 pKa = 11.84GTKK336 pKa = 10.07FYY338 pKa = 10.52INSLEE343 pKa = 4.77KK344 pKa = 9.42EE345 pKa = 4.11NKK347 pKa = 8.35YY348 pKa = 10.73NKK350 pKa = 9.95HH351 pKa = 5.56FLASLKK357 pKa = 10.82EE358 pKa = 4.01FDD360 pKa = 4.03TKK362 pKa = 11.54SNLAVEE368 pKa = 4.59EE369 pKa = 4.11FRR371 pKa = 11.84NQFKK375 pKa = 10.87VLSTTRR381 pKa = 11.84RR382 pKa = 11.84RR383 pKa = 11.84KK384 pKa = 9.64LGNKK388 pKa = 8.87IVVEE392 pKa = 4.84FKK394 pKa = 11.02SGDD397 pKa = 3.09TRR399 pKa = 11.84FYY401 pKa = 11.49GRR403 pKa = 11.84IPDD406 pKa = 3.51ATKK409 pKa = 10.46FNSKK413 pKa = 10.03FGNKK417 pKa = 9.16FQDD420 pKa = 3.43FRR422 pKa = 11.84KK423 pKa = 6.3TTKK426 pKa = 9.89NVKK429 pKa = 9.3LVKK432 pKa = 9.65TIKK435 pKa = 10.64KK436 pKa = 9.88LL437 pKa = 3.44

Molecular weight: 50.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15

0

15

4733

91

766

315.5

36.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.521 ± 0.457

0.739 ± 0.151

5.514 ± 0.42

7.057 ± 0.58

5.958 ± 0.299

3.528 ± 0.426

1.458 ± 0.143

8.684 ± 0.271

11.261 ± 0.567

9.719 ± 0.454

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.563 ± 0.204

8.494 ± 0.519

2.704 ± 0.218

3.444 ± 0.267

2.895 ± 0.286

6.529 ± 0.325

5.599 ± 0.523

4.606 ± 0.475

1.373 ± 0.182

4.352 ± 0.415
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski