Adonis mosaic virus
Average proteome isoelectric point is 8.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1J1E0V5|A0A1J1E0V5_9TOMB p7K OS=Adonis mosaic virus OX=1883104 GN=ORF5 PE=4 SV=1
MM1 pKa = 7.6 GFLKK5 pKa = 10.47 FATEE9 pKa = 4.0 LAVGSTLMGGLVSVGIAGLTLRR31 pKa = 11.84 ATIGVVEE38 pKa = 4.8 FEE40 pKa = 3.92 RR41 pKa = 11.84 RR42 pKa = 11.84 IIVGTCKK49 pKa = 10.28 YY50 pKa = 9.36 VASRR54 pKa = 11.84 GLSLPSHH61 pKa = 7.0 PEE63 pKa = 3.56 YY64 pKa = 10.4 PKK66 pKa = 11.19 NSDD69 pKa = 3.25 QLFLEE74 pKa = 4.77 EE75 pKa = 4.29 EE76 pKa = 4.43 SEE78 pKa = 4.51 SLSEE82 pKa = 4.13 SQQVVEE88 pKa = 5.47 DD89 pKa = 3.8 FTEE92 pKa = 4.13 LVEE95 pKa = 4.88 EE96 pKa = 4.33 KK97 pKa = 11.26 GEE99 pKa = 4.09 DD100 pKa = 3.55 KK101 pKa = 10.73 EE102 pKa = 4.36 GKK104 pKa = 9.76 EE105 pKa = 4.01 VVISQKK111 pKa = 10.52 RR112 pKa = 11.84 VINRR116 pKa = 11.84 HH117 pKa = 5.32 SKK119 pKa = 10.44 SKK121 pKa = 9.88 FIRR124 pKa = 11.84 YY125 pKa = 8.6 IAIEE129 pKa = 4.22 AKK131 pKa = 10.15 NHH133 pKa = 6.75 FGGDD137 pKa = 3.58 LCASKK142 pKa = 11.13 ANYY145 pKa = 10.25 LSVSKK150 pKa = 10.47 FLTGKK155 pKa = 9.81 CKK157 pKa = 9.96 EE158 pKa = 4.22 RR159 pKa = 11.84 QVVPAHH165 pKa = 5.77 TRR167 pKa = 11.84 VCVASAMALVFTPDD181 pKa = 3.03 MAEE184 pKa = 3.72 VQMRR188 pKa = 11.84 VALNSHH194 pKa = 5.54 EE195 pKa = 4.13 AYY197 pKa = 9.7 RR198 pKa = 11.84 RR199 pKa = 11.84 RR200 pKa = 11.84 VAMEE204 pKa = 3.79 AAGSMSSWGMRR215 pKa = 11.84 LIQNPLTNSAWEE227 pKa = 4.08 EE228 pKa = 3.26 AWYY231 pKa = 10.33 VLNGRR236 pKa = 11.84 SSRR239 pKa = 11.84 SAVTFTKK246 pKa = 10.64 GGLFYY251 pKa = 11.38 LEE253 pKa = 4.35 GVEE256 pKa = 4.01 TAIRR260 pKa = 11.84 RR261 pKa = 11.84 GSHH264 pKa = 6.03 PSMIEE269 pKa = 3.93 VEE271 pKa = 4.66 LKK273 pKa = 10.74 APPKK277 pKa = 10.03 LRR279 pKa = 11.84 KK280 pKa = 9.73 LYY282 pKa = 9.57 VQNSLSSGFDD292 pKa = 3.25 YY293 pKa = 10.33 RR294 pKa = 11.84 VHH296 pKa = 5.42 NHH298 pKa = 5.91 SYY300 pKa = 9.54 QNLRR304 pKa = 11.84 RR305 pKa = 11.84 GLLEE309 pKa = 3.47 RR310 pKa = 11.84 VFYY313 pKa = 11.26 VEE315 pKa = 5.25 SKK317 pKa = 10.77 DD318 pKa = 3.93 KK319 pKa = 11.08 EE320 pKa = 4.32 LVGCPRR326 pKa = 11.84 PKK328 pKa = 10.2 PGAFKK333 pKa = 10.6 EE334 pKa = 3.83 LGYY337 pKa = 10.43 LRR339 pKa = 11.84 RR340 pKa = 11.84 RR341 pKa = 11.84 FHH343 pKa = 7.32 RR344 pKa = 11.84 ICGNHH349 pKa = 4.54 TRR351 pKa = 11.84 ISAEE355 pKa = 3.95 EE356 pKa = 4.05 FVDD359 pKa = 4.84 CYY361 pKa = 10.52 QGRR364 pKa = 11.84 KK365 pKa = 6.25 RR366 pKa = 11.84 TIYY369 pKa = 10.36 EE370 pKa = 3.89 NARR373 pKa = 11.84 LSLDD377 pKa = 3.65 DD378 pKa = 4.24 KK379 pKa = 11.47 ALEE382 pKa = 4.63 RR383 pKa = 11.84 KK384 pKa = 10.31 DD385 pKa = 4.56 GDD387 pKa = 3.54 LKK389 pKa = 10.58 TFIKK393 pKa = 10.68 AEE395 pKa = 3.98 KK396 pKa = 10.05 FNISVKK402 pKa = 9.93 PDD404 pKa = 3.51 PAPRR408 pKa = 11.84 VIQPRR413 pKa = 11.84 SPRR416 pKa = 11.84 FNLEE420 pKa = 3.47 LGRR423 pKa = 11.84 YY424 pKa = 7.93 LKK426 pKa = 10.67 KK427 pKa = 10.66 FEE429 pKa = 4.61 HH430 pKa = 6.28 NAYY433 pKa = 9.74 KK434 pKa = 10.49 AIDD437 pKa = 4.56 KK438 pKa = 10.37 IWGAPTIMKK447 pKa = 10.17 GYY449 pKa = 8.22 NTEE452 pKa = 3.9 EE453 pKa = 4.12 VAQHH457 pKa = 6.81 LKK459 pKa = 10.45 HH460 pKa = 6.61 AWDD463 pKa = 3.5 QFQIPVAVGFDD474 pKa = 3.25 MSRR477 pKa = 11.84 FDD479 pKa = 3.32 QHH481 pKa = 7.94 VSVPALEE488 pKa = 4.93 FEE490 pKa = 4.51 HH491 pKa = 7.0 SCYY494 pKa = 10.02 TSCFEE499 pKa = 4.98 GDD501 pKa = 3.18 QHH503 pKa = 7.76 LADD506 pKa = 4.48 LLKK509 pKa = 10.13 MQLKK513 pKa = 8.85 NHH515 pKa = 5.75 GVGFASNGMIRR526 pKa = 11.84 YY527 pKa = 6.39 TKK529 pKa = 9.82 EE530 pKa = 3.57 GCRR533 pKa = 11.84 MSGDD537 pKa = 3.52 MNTALGNCLLACLITKK553 pKa = 10.13 HH554 pKa = 4.93 ITRR557 pKa = 11.84 GVRR560 pKa = 11.84 GKK562 pKa = 10.71 LINNGDD568 pKa = 3.87 DD569 pKa = 3.53 CVFICEE575 pKa = 4.19 RR576 pKa = 11.84 SDD578 pKa = 3.32 AGSVVSDD585 pKa = 4.49 LTTGWASFGFNCIAEE600 pKa = 4.47 TPVEE604 pKa = 4.24 VFEE607 pKa = 5.16 EE608 pKa = 4.04 IRR610 pKa = 11.84 FCQMAPVFDD619 pKa = 4.13 GEE621 pKa = 4.46 VWVMVRR627 pKa = 11.84 DD628 pKa = 4.2 PKK630 pKa = 11.42 VSMSKK635 pKa = 10.19 DD636 pKa = 3.13 AHH638 pKa = 7.1 SLVHH642 pKa = 6.29 WNNEE646 pKa = 3.59 RR647 pKa = 11.84 NALQWFKK654 pKa = 11.37 AVGQCGMKK662 pKa = 9.78 IAGRR666 pKa = 11.84 VPVVQEE672 pKa = 3.95 YY673 pKa = 7.1 YY674 pKa = 10.31 QKK676 pKa = 10.92 YY677 pKa = 9.14 IEE679 pKa = 4.5 TGGDD683 pKa = 3.3 VKK685 pKa = 10.43 TSGSLSEE692 pKa = 4.2 QVGGTGMFRR701 pKa = 11.84 MAANSTRR708 pKa = 11.84 GYY710 pKa = 10.73 GAISQEE716 pKa = 3.76 ARR718 pKa = 11.84 YY719 pKa = 10.25 SFYY722 pKa = 10.85 LAFGIQPDD730 pKa = 3.67 EE731 pKa = 4.37 QIALEE736 pKa = 4.01 HH737 pKa = 7.12 EE738 pKa = 4.1 IRR740 pKa = 11.84 KK741 pKa = 5.64 TTIHH745 pKa = 6.23 ATLGPQCEE753 pKa = 4.56 SADD756 pKa = 3.9 SIQWLINPNLTT767 pKa = 3.6
Molecular weight: 86.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.285
IPC2_protein 7.673
IPC_protein 7.468
Toseland 7.146
ProMoST 8.083
Dawson 8.214
Bjellqvist 8.609
Wikipedia 8.126
Rodwell 8.244
Grimsley 7.22
Solomon 8.244
Lehninger 8.273
Nozaki 8.829
DTASelect 8.317
Thurlkill 8.39
EMBOSS 8.419
Sillero 8.712
Patrickios 4.418
IPC_peptide 8.244
IPC2_peptide 7.571
IPC2.peptide.svr19 7.671
Protein with the highest isoelectric point:
>tr|A0A1J1DWM6|A0A1J1DWM6_9TOMB RNA-directed RNA polymerase OS=Adonis mosaic virus OX=1883104 GN=ORF1RT PE=4 SV=1
MM1 pKa = 7.74 PSANQNLTVITGVIGLLWLMRR22 pKa = 11.84 SRR24 pKa = 11.84 SILHH28 pKa = 6.65 SIFEE32 pKa = 4.26 AAKK35 pKa = 9.71 PPPLLTLSQIIVFSFCGLLLNCISRR60 pKa = 11.84 AEE62 pKa = 4.03 RR63 pKa = 11.84 EE64 pKa = 4.14 VVITNNHH71 pKa = 6.62 DD72 pKa = 3.49 SSKK75 pKa = 9.64 QQHH78 pKa = 5.51 ITVNTPSKK86 pKa = 10.95
Molecular weight: 9.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.67
IPC2_protein 8.434
IPC_protein 8.668
Toseland 9.311
ProMoST 9.443
Dawson 9.37
Bjellqvist 9.385
Wikipedia 9.355
Rodwell 9.692
Grimsley 8.931
Solomon 9.765
Lehninger 9.794
Nozaki 9.823
DTASelect 9.194
Thurlkill 9.399
EMBOSS 9.663
Sillero 9.604
Patrickios 10.35
IPC_peptide 9.765
IPC2_peptide 8.492
IPC2.peptide.svr19 8.064
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1576
64
767
262.7
29.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.741 ± 0.561
1.904 ± 0.413
3.744 ± 0.699
6.98 ± 0.911
4.569 ± 0.375
7.424 ± 0.77
2.411 ± 0.502
4.569 ± 0.821
6.091 ± 0.315
7.868 ± 0.587
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.538 ± 0.246
4.251 ± 0.711
3.934 ± 0.568
3.49 ± 0.276
6.218 ± 0.426
8.249 ± 0.829
5.266 ± 0.724
8.566 ± 0.678
1.586 ± 0.312
2.538 ± 0.616
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here