Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; Halobacteriaceae; Halobacterium; Halobacterium salinarum

Average proteome isoelectric point is 5.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2426 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9HPA0|Q9HPA0_HALSA MOSC domain protein OS=Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) OX=64091 GN=VNG_1735C PE=4 SV=1
MM1 pKa = 7.58SEE3 pKa = 3.81TANADD8 pKa = 3.79LYY10 pKa = 10.94RR11 pKa = 11.84DD12 pKa = 4.03TVALLQPGDD21 pKa = 3.66VTLAGAVIHH30 pKa = 5.48TTYY33 pKa = 11.53DD34 pKa = 3.2NDD36 pKa = 3.93EE37 pKa = 4.21EE38 pKa = 5.05SKK40 pKa = 10.78LHH42 pKa = 6.52QLTLDD47 pKa = 3.76AGQVVADD54 pKa = 4.72HH55 pKa = 6.37VADD58 pKa = 4.17GDD60 pKa = 4.22TYY62 pKa = 11.31VYY64 pKa = 10.7SGNDD68 pKa = 3.28DD69 pKa = 3.36SDD71 pKa = 4.22FGVNQHH77 pKa = 5.89QGRR80 pKa = 11.84ILDD83 pKa = 4.08DD84 pKa = 4.51DD85 pKa = 4.35AFVWEE90 pKa = 4.67CQQLLRR96 pKa = 11.84DD97 pKa = 3.57GAFAVVLYY105 pKa = 9.94WEE107 pKa = 4.63ATDD110 pKa = 3.61DD111 pKa = 3.88HH112 pKa = 6.98AAILDD117 pKa = 4.6GIRR120 pKa = 11.84DD121 pKa = 4.03CDD123 pKa = 3.58GVTSVVAVTEE133 pKa = 4.56DD134 pKa = 3.32GFEE137 pKa = 3.86AA138 pKa = 5.01

Molecular weight: 14.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9HQA7|Q9HQA7_HALSA ABC-type transport system permease protein OS=Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) OX=64091 GN=VNG_1249C PE=4 SV=1
MM1 pKa = 7.94ADD3 pKa = 3.54GGDD6 pKa = 3.67VNHH9 pKa = 6.77VIVKK13 pKa = 9.78AARR16 pKa = 11.84THH18 pKa = 5.35GVRR21 pKa = 11.84LEE23 pKa = 3.76FDD25 pKa = 3.36FAGVLRR31 pKa = 11.84ASGGDD36 pKa = 2.87RR37 pKa = 11.84VQALRR42 pKa = 11.84GLRR45 pKa = 11.84KK46 pKa = 9.46LRR48 pKa = 11.84EE49 pKa = 4.12LVEE52 pKa = 5.2HH53 pKa = 6.67YY54 pKa = 8.5DD55 pKa = 3.42APFVVSGRR63 pKa = 11.84PASHH67 pKa = 6.28LHH69 pKa = 4.75VRR71 pKa = 11.84SPRR74 pKa = 11.84EE75 pKa = 3.74LVAVGAEE82 pKa = 3.82IGFTDD87 pKa = 3.56AQVRR91 pKa = 11.84AGLRR95 pKa = 11.84EE96 pKa = 4.17WTHH99 pKa = 5.37LAARR103 pKa = 11.84NRR105 pKa = 11.84RR106 pKa = 11.84RR107 pKa = 11.84LSAEE111 pKa = 4.41FIAPGVKK118 pKa = 9.26RR119 pKa = 11.84GRR121 pKa = 11.84YY122 pKa = 9.3EE123 pKa = 3.8EE124 pKa = 4.93DD125 pKa = 3.04PP126 pKa = 4.0

Molecular weight: 13.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2423

3

2426

680494

30

1370

280.5

30.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.657 ± 0.095

0.749 ± 0.014

8.943 ± 0.059

6.902 ± 0.074

3.115 ± 0.033

8.333 ± 0.052

2.241 ± 0.026

3.726 ± 0.042

1.742 ± 0.03

8.461 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.739 ± 0.021

2.212 ± 0.029

4.69 ± 0.032

2.728 ± 0.033

6.521 ± 0.058

5.372 ± 0.038

6.826 ± 0.042

9.398 ± 0.058

1.091 ± 0.019

2.553 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski