Roseiflexus sp. (strain RS-1)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; Chloroflexia; Chloroflexales; Roseiflexineae; Roseiflexaceae; Roseiflexus; unclassified Roseiflexus

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4494 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A5V179|A5V179_ROSS1 Uncharacterized protein OS=Roseiflexus sp. (strain RS-1) OX=357808 GN=RoseRS_4291 PE=4 SV=1
MM1 pKa = 7.23SASLKK6 pKa = 10.81SPATLFVLGVFLIMTAVLPAPRR28 pKa = 11.84PVSASSVLYY37 pKa = 9.97AAPTATGSADD47 pKa = 3.31CSSWANACTLQTALTNATSGNQIWVRR73 pKa = 11.84QGVHH77 pKa = 6.33RR78 pKa = 11.84PGSSQTDD85 pKa = 3.41TFTLKK90 pKa = 10.89NGVAVYY96 pKa = 10.52GGFAGTEE103 pKa = 3.78TSLAQRR109 pKa = 11.84DD110 pKa = 3.54WQTNVTVLSGDD121 pKa = 3.4INQDD125 pKa = 3.98DD126 pKa = 3.77ITDD129 pKa = 3.74ANGVVTSTANIAGVNAYY146 pKa = 9.87HH147 pKa = 6.17VVTGGGTNNTAVLDD161 pKa = 4.15GFTITAGNANSPSPPDD177 pKa = 3.6DD178 pKa = 4.49AGGGMYY184 pKa = 10.65NNNSSPTLRR193 pKa = 11.84NLIFSGNAANSGGGMANFNSSNPTLTNVTFSGNSADD229 pKa = 5.09LGGGMYY235 pKa = 10.48NEE237 pKa = 4.6NSSNPTLTNVTFSSNTAQSGGGMYY261 pKa = 10.54NFYY264 pKa = 10.94SSPTLTNVTFSGNAAQAGGGMFNSNSNPVLTNVTFSGNTASGMGSSGGGMFNYY317 pKa = 10.29DD318 pKa = 3.26NSSPTLTNVTFSSNTAQSGGGMYY341 pKa = 10.68NFFDD345 pKa = 4.69SSPTLTNVIFSGNAAQAGGGMFNEE369 pKa = 4.3NSSPILTNVIFSGNAVTVSGGGMANFNGSNPNLINVTFSGNTASSGGGMANFNGSNPTLTNVIIWNSVGGSIINSSSTPDD449 pKa = 3.17VTYY452 pKa = 11.41SNIQGGYY459 pKa = 10.09SGTTNINADD468 pKa = 4.07PFFVDD473 pKa = 5.16ADD475 pKa = 3.91GPDD478 pKa = 4.01NIVGTLDD485 pKa = 3.81DD486 pKa = 4.18NLRR489 pKa = 11.84LQSGSPSIDD498 pKa = 3.03TGNNAAVPTGVTTDD512 pKa = 3.95LEE514 pKa = 4.5GKK516 pKa = 9.52PRR518 pKa = 11.84IQNGTVDD525 pKa = 3.58MGAYY529 pKa = 9.6EE530 pKa = 4.45YY531 pKa = 11.04DD532 pKa = 3.52NTPPTVVSITRR543 pKa = 11.84ADD545 pKa = 3.8PNPTNAASVTFVVTFTEE562 pKa = 4.23AVTGVGVADD571 pKa = 4.9FALTLTGGVSGASVTGVSGSGAVYY595 pKa = 9.19TVTVSTGTGDD605 pKa = 3.09GTLRR609 pKa = 11.84LDD611 pKa = 3.76IPNTATITDD620 pKa = 4.17LAGNALSGLPFTGGQVYY637 pKa = 7.87TVDD640 pKa = 3.05KK641 pKa = 8.75TAPGVTMTSSAPNPTNSAPIPVTVTFSEE669 pKa = 4.62PVTGFTAGDD678 pKa = 3.37IAVSNGSVSNFAGGGASYY696 pKa = 9.93TFDD699 pKa = 3.82LTPAANGLVTATIAANVAVDD719 pKa = 3.33AAGNGNTAASFSRR732 pKa = 11.84TYY734 pKa = 10.38DD735 pKa = 3.07TTAPGVASITRR746 pKa = 11.84ADD748 pKa = 4.0PNPTSAASVNFIVTFTEE765 pKa = 3.94AVTGVGVADD774 pKa = 4.85FALTVTGISGASVTGVSGSGAIYY797 pKa = 9.46TVTVSTGTGDD807 pKa = 3.09GTLRR811 pKa = 11.84LDD813 pKa = 3.4IPTAATITDD822 pKa = 4.1LAGNALSGLPYY833 pKa = 10.31TSGEE837 pKa = 4.46TYY839 pKa = 9.9QKK841 pKa = 10.57GYY843 pKa = 10.83RR844 pKa = 11.84VFLPLIVRR852 pKa = 4.42

Molecular weight: 85.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A5UVQ2|A5UVQ2_ROSS1 Uncharacterized protein OS=Roseiflexus sp. (strain RS-1) OX=357808 GN=RoseRS_2326 PE=4 SV=1
MM1 pKa = 7.55CMSDD5 pKa = 4.12YY6 pKa = 10.67LTVAPPRR13 pKa = 11.84RR14 pKa = 11.84LRR16 pKa = 11.84LHH18 pKa = 6.82ASVQIAGMRR27 pKa = 11.84LPARR31 pKa = 11.84AFLMSVGILTAGGIAITLGADD52 pKa = 3.22IEE54 pKa = 4.37RR55 pKa = 11.84TIWASGCLIFLGLALLEE72 pKa = 4.1GRR74 pKa = 11.84VWGRR78 pKa = 11.84SSRR81 pKa = 11.84EE82 pKa = 3.37AAGIVWRR89 pKa = 11.84HH90 pKa = 5.25LKK92 pKa = 10.06RR93 pKa = 11.84PKK95 pKa = 9.97RR96 pKa = 11.84LRR98 pKa = 11.84LIQPHH103 pKa = 5.85MALPPEE109 pKa = 4.51EE110 pKa = 4.46ALSPVLKK117 pKa = 9.98PRR119 pKa = 11.84RR120 pKa = 11.84PHH122 pKa = 4.05WHH124 pKa = 5.17YY125 pKa = 11.22QEE127 pKa = 4.37TADD130 pKa = 4.63EE131 pKa = 4.43HH132 pKa = 6.99DD133 pKa = 3.48

Molecular weight: 14.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4494

0

4494

1633847

38

5166

363.6

39.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.859 ± 0.055

0.877 ± 0.012

5.445 ± 0.028

5.548 ± 0.041

3.328 ± 0.022

7.649 ± 0.037

2.18 ± 0.019

5.602 ± 0.026

1.852 ± 0.028

10.797 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.106 ± 0.014

2.494 ± 0.026

5.969 ± 0.032

3.651 ± 0.024

8.281 ± 0.043

4.832 ± 0.027

5.723 ± 0.045

7.618 ± 0.035

1.545 ± 0.016

2.647 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski