Amino acid dipepetide frequency for Roseiflexus sp. (strain RS-1)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	15.389AlaAla: 15.389 ± 0.191	1.151AlaCys: 1.151 ± 0.028	5.821AlaAsp: 5.821 ± 0.056	6.375AlaGlu: 6.375 ± 0.084	3.796AlaPhe: 3.796 ± 0.053	8.749AlaGly: 8.749 ± 0.105	2.522AlaHis: 2.522 ± 0.045	6.592AlaIle: 6.592 ± 0.076	1.716AlaLys: 1.716 ± 0.039	15.596AlaLeu: 15.596 ± 0.177	2.458AlaMet: 2.458 ± 0.046	2.287AlaAsn: 2.287 ± 0.04	7.052AlaPro: 7.052 ± 0.099	4.601AlaGln: 4.601 ± 0.06	9.518AlaArg: 9.518 ± 0.101	5.403AlaSer: 5.403 ± 0.066	6.804AlaThr: 6.804 ± 0.09	8.114AlaVal: 8.114 ± 0.085	1.779AlaTrp: 1.779 ± 0.039	2.864AlaTyr: 2.864 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	1.034CysAla: 1.034 ± 0.029	0.121CysCys: 0.121 ± 0.009	0.572CysAsp: 0.572 ± 0.02	0.419CysGlu: 0.419 ± 0.018	0.305CysPhe: 0.305 ± 0.014	0.966CysGly: 0.966 ± 0.028	0.226CysHis: 0.226 ± 0.013	0.401CysIle: 0.401 ± 0.013	0.141CysLys: 0.141 ± 0.009	0.741CysLeu: 0.741 ± 0.022	0.173CysMet: 0.173 ± 0.011	0.252CysAsn: 0.252 ± 0.013	0.544CysPro: 0.544 ± 0.021	0.276CysGln: 0.276 ± 0.014	0.659CysArg: 0.659 ± 0.022	0.436CysSer: 0.436 ± 0.018	0.488CysThr: 0.488 ± 0.019	0.583CysVal: 0.583 ± 0.021	0.16CysTrp: 0.16 ± 0.014	0.27CysTyr: 0.27 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	6.447AspAla: 6.447 ± 0.074	0.362AspCys: 0.362 ± 0.016	3.379AspAsp: 3.379 ± 0.061	3.627AspGlu: 3.627 ± 0.057	1.591AspPhe: 1.591 ± 0.033	4.455AspGly: 4.455 ± 0.076	1.261AspHis: 1.261 ± 0.029	2.887AspIle: 2.887 ± 0.042	0.758AspLys: 0.758 ± 0.023	6.126AspLeu: 6.126 ± 0.072	1.101AspMet: 1.101 ± 0.023	1.146AspAsn: 1.146 ± 0.034	3.82AspPro: 3.82 ± 0.049	1.988AspGln: 1.988 ± 0.035	4.095AspArg: 4.095 ± 0.048	1.904AspSer: 1.904 ± 0.034	2.648AspThr: 2.648 ± 0.045	4.522AspVal: 4.522 ± 0.062	0.852AspTrp: 0.852 ± 0.023	1.479AspTyr: 1.479 ± 0.03	0.0AspXaa: 0.0 ± 0.0
Glu	6.665GluAla: 6.665 ± 0.092	0.381GluCys: 0.381 ± 0.015	2.038GluAsp: 2.038 ± 0.037	3.496GluGlu: 3.496 ± 0.071	1.508GluPhe: 1.508 ± 0.032	3.802GluGly: 3.802 ± 0.053	1.336GluHis: 1.336 ± 0.031	2.954GluIle: 2.954 ± 0.047	1.331GluLys: 1.331 ± 0.033	5.358GluLeu: 5.358 ± 0.071	1.288GluMet: 1.288 ± 0.027	1.167GluAsn: 1.167 ± 0.028	2.806GluPro: 2.806 ± 0.049	2.957GluGln: 2.957 ± 0.056	6.717GluArg: 6.717 ± 0.078	2.31GluSer: 2.31 ± 0.045	3.328GluThr: 3.328 ± 0.05	3.692GluVal: 3.692 ± 0.058	0.888GluTrp: 0.888 ± 0.029	1.455GluTyr: 1.455 ± 0.031	0.0GluXaa: 0.0 ± 0.0
Phe	3.73PheAla: 3.73 ± 0.052	0.371PheCys: 0.371 ± 0.016	2.32PheAsp: 2.32 ± 0.036	1.848PheGlu: 1.848 ± 0.035	1.345PhePhe: 1.345 ± 0.032	3.019PheGly: 3.019 ± 0.05	0.663PheHis: 0.663 ± 0.022	1.711PheIle: 1.711 ± 0.032	0.556PheLys: 0.556 ± 0.018	3.304PheLeu: 3.304 ± 0.047	0.523PheMet: 0.523 ± 0.019	0.993PheAsn: 0.993 ± 0.027	1.667PhePro: 1.667 ± 0.033	0.885PheGln: 0.885 ± 0.023	2.257PheArg: 2.257 ± 0.038	1.825PheSer: 1.825 ± 0.037	1.97PheThr: 1.97 ± 0.043	2.703PheVal: 2.703 ± 0.048	0.608PheTrp: 0.608 ± 0.02	0.979PheTyr: 0.979 ± 0.026	0.0PheXaa: 0.0 ± 0.0
Gly	8.886GlyAla: 8.886 ± 0.1	0.806GlyCys: 0.806 ± 0.026	4.267GlyAsp: 4.267 ± 0.068	4.027GlyGlu: 4.027 ± 0.049	2.727GlyPhe: 2.727 ± 0.051	6.213GlyGly: 6.213 ± 0.108	1.483GlyHis: 1.483 ± 0.03	4.644GlyIle: 4.644 ± 0.053	2.08GlyLys: 2.08 ± 0.041	7.255GlyLeu: 7.255 ± 0.085	2.209GlyMet: 2.209 ± 0.038	2.014GlyAsn: 2.014 ± 0.052	2.7GlyPro: 2.7 ± 0.048	2.692GlyGln: 2.692 ± 0.043	6.085GlyArg: 6.085 ± 0.075	4.087GlySer: 4.087 ± 0.073	3.763GlyThr: 3.763 ± 0.086	6.704GlyVal: 6.704 ± 0.085	1.427GlyTrp: 1.427 ± 0.028	2.421GlyTyr: 2.421 ± 0.046	0.0GlyXaa: 0.0 ± 0.0
His	2.396HisAla: 2.396 ± 0.044	0.215HisCys: 0.215 ± 0.012	1.232HisAsp: 1.232 ± 0.033	1.1HisGlu: 1.1 ± 0.029	0.688HisPhe: 0.688 ± 0.024	1.702HisGly: 1.702 ± 0.035	0.683HisHis: 0.683 ± 0.021	1.23HisIle: 1.23 ± 0.03	0.35HisLys: 0.35 ± 0.014	2.527HisLeu: 2.527 ± 0.052	0.422HisMet: 0.422 ± 0.014	0.559HisAsn: 0.559 ± 0.019	1.72HisPro: 1.72 ± 0.036	0.749HisGln: 0.749 ± 0.023	1.61HisArg: 1.61 ± 0.037	0.91HisSer: 0.91 ± 0.025	1.285HisThr: 1.285 ± 0.032	1.485HisVal: 1.485 ± 0.035	0.304HisTrp: 0.304 ± 0.016	0.634HisTyr: 0.634 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	7.44IleAla: 7.44 ± 0.086	0.57IleCys: 0.57 ± 0.019	3.802IleAsp: 3.802 ± 0.048	3.786IleGlu: 3.786 ± 0.053	1.663IlePhe: 1.663 ± 0.038	5.14IleGly: 5.14 ± 0.069	1.091IleHis: 1.091 ± 0.026	3.062IleIle: 3.062 ± 0.047	1.061IleLys: 1.061 ± 0.025	4.686IleLeu: 4.686 ± 0.055	0.856IleMet: 0.856 ± 0.024	1.591IleAsn: 1.591 ± 0.032	2.974IlePro: 2.974 ± 0.043	1.557IleGln: 1.557 ± 0.035	3.661IleArg: 3.661 ± 0.046	2.661IleSer: 2.661 ± 0.048	3.232IleThr: 3.232 ± 0.068	4.983IleVal: 4.983 ± 0.063	0.771IleTrp: 0.771 ± 0.022	1.427IleTyr: 1.427 ± 0.03	0.0IleXaa: 0.0 ± 0.0
Lys	1.848LysAla: 1.848 ± 0.037	0.121LysCys: 0.121 ± 0.009	0.851LysAsp: 0.851 ± 0.027	1.089LysGlu: 1.089 ± 0.031	0.45LysPhe: 0.45 ± 0.018	1.319LysGly: 1.319 ± 0.034	0.406LysHis: 0.406 ± 0.017	1.07LysIle: 1.07 ± 0.025	0.566LysLys: 0.566 ± 0.022	1.755LysLeu: 1.755 ± 0.035	0.433LysMet: 0.433 ± 0.018	0.562LysAsn: 0.562 ± 0.022	1.326LysPro: 1.326 ± 0.034	0.816LysGln: 0.816 ± 0.022	1.704LysArg: 1.704 ± 0.038	0.886LysSer: 0.886 ± 0.029	1.181LysThr: 1.181 ± 0.029	1.442LysVal: 1.442 ± 0.036	0.219LysTrp: 0.219 ± 0.013	0.471LysTyr: 0.471 ± 0.018	0.0LysXaa: 0.0 ± 0.0
Leu	13.829LeuAla: 13.829 ± 0.146	0.906LeuCys: 0.906 ± 0.025	5.865LeuAsp: 5.865 ± 0.065	5.661LeuGlu: 5.661 ± 0.067	3.645LeuPhe: 3.645 ± 0.052	7.111LeuGly: 7.111 ± 0.084	2.325LeuHis: 2.325 ± 0.037	6.621LeuIle: 6.621 ± 0.072	2.155LeuLys: 2.155 ± 0.043	11.931LeuLeu: 11.931 ± 0.141	2.072LeuMet: 2.072 ± 0.04	2.739LeuAsn: 2.739 ± 0.041	6.728LeuPro: 6.728 ± 0.075	3.387LeuGln: 3.387 ± 0.053	9.462LeuArg: 9.462 ± 0.074	5.476LeuSer: 5.476 ± 0.059	6.549LeuThr: 6.549 ± 0.066	7.472LeuVal: 7.472 ± 0.085	1.454LeuTrp: 1.454 ± 0.038	2.58LeuTyr: 2.58 ± 0.04	0.0LeuXaa: 0.0 ± 0.0
Met	2.395MetAla: 2.395 ± 0.044	0.159MetCys: 0.159 ± 0.01	0.766MetAsp: 0.766 ± 0.023	0.911MetGlu: 0.911 ± 0.023	0.629MetPhe: 0.629 ± 0.022	1.29MetGly: 1.29 ± 0.029	0.501MetHis: 0.501 ± 0.018	1.222MetIle: 1.222 ± 0.029	0.523MetLys: 0.523 ± 0.017	2.456MetLeu: 2.456 ± 0.041	0.495MetMet: 0.495 ± 0.018	0.626MetAsn: 0.626 ± 0.02	1.415MetPro: 1.415 ± 0.032	0.799MetGln: 0.799 ± 0.025	1.936MetArg: 1.936 ± 0.034	1.246MetSer: 1.246 ± 0.027	1.498MetThr: 1.498 ± 0.032	1.51MetVal: 1.51 ± 0.031	0.239MetTrp: 0.239 ± 0.016	0.443MetTyr: 0.443 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	2.791AsnAla: 2.791 ± 0.051	0.177AsnCys: 0.177 ± 0.012	1.45AsnAsp: 1.45 ± 0.037	1.289AsnGlu: 1.289 ± 0.032	0.687AsnPhe: 0.687 ± 0.025	2.293AsnGly: 2.293 ± 0.061	0.509AsnHis: 0.509 ± 0.019	1.266AsnIle: 1.266 ± 0.027	0.404AsnLys: 0.404 ± 0.015	2.75AsnLeu: 2.75 ± 0.043	0.44AsnMet: 0.44 ± 0.017	0.795AsnAsn: 0.795 ± 0.04	1.998AsnPro: 1.998 ± 0.044	0.875AsnGln: 0.875 ± 0.027	2.001AsnArg: 2.001 ± 0.04	0.921AsnSer: 0.921 ± 0.026	1.34AsnThr: 1.34 ± 0.045	1.936AsnVal: 1.936 ± 0.039	0.378AsnTrp: 0.378 ± 0.016	0.644AsnTyr: 0.644 ± 0.021	0.0AsnXaa: 0.0 ± 0.0
Pro	6.616ProAla: 6.616 ± 0.089	0.367ProCys: 0.367 ± 0.018	4.404ProAsp: 4.404 ± 0.059	3.741ProGlu: 3.741 ± 0.062	2.052ProPhe: 2.052 ± 0.032	4.956ProGly: 4.956 ± 0.08	1.367ProHis: 1.367 ± 0.037	2.546ProIle: 2.546 ± 0.04	0.911ProLys: 0.911 ± 0.03	6.029ProLeu: 6.029 ± 0.063	1.079ProMet: 1.079 ± 0.025	1.436ProAsn: 1.436 ± 0.039	4.534ProPro: 4.534 ± 0.098	2.122ProGln: 2.122 ± 0.042	3.615ProArg: 3.615 ± 0.049	3.016ProSer: 3.016 ± 0.056	3.758ProThr: 3.758 ± 0.103	4.721ProVal: 4.721 ± 0.07	0.895ProTrp: 0.895 ± 0.025	1.526ProTyr: 1.526 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	4.478GlnAla: 4.478 ± 0.063	0.272GlnCys: 0.272 ± 0.015	1.168GlnAsp: 1.168 ± 0.028	1.935GlnGlu: 1.935 ± 0.043	1.077GlnPhe: 1.077 ± 0.029	2.31GlnGly: 2.31 ± 0.049	0.876GlnHis: 0.876 ± 0.025	2.258GlnIle: 2.258 ± 0.042	0.783GlnLys: 0.783 ± 0.026	2.992GlnLeu: 2.992 ± 0.051	0.862GlnMet: 0.862 ± 0.022	0.848GlnAsn: 0.848 ± 0.021	2.501GlnPro: 2.501 ± 0.046	1.938GlnGln: 1.938 ± 0.064	4.082GlnArg: 4.082 ± 0.061	1.784GlnSer: 1.784 ± 0.034	2.142GlnThr: 2.142 ± 0.041	2.826GlnVal: 2.826 ± 0.049	0.534GlnTrp: 0.534 ± 0.023	0.839GlnTyr: 0.839 ± 0.022	0.0GlnXaa: 0.0 ± 0.0
Arg	9.248ArgAla: 9.248 ± 0.11	0.75ArgCys: 0.75 ± 0.021	4.512ArgAsp: 4.512 ± 0.05	4.748ArgGlu: 4.748 ± 0.068	3.015ArgPhe: 3.015 ± 0.045	5.06ArgGly: 5.06 ± 0.062	1.852ArgHis: 1.852 ± 0.038	4.636ArgIle: 4.636 ± 0.055	1.554ArgLys: 1.554 ± 0.038	9.662ArgLeu: 9.662 ± 0.082	2.113ArgMet: 2.113 ± 0.043	2.056ArgAsn: 2.056 ± 0.04	4.074ArgPro: 4.074 ± 0.051	3.471ArgGln: 3.471 ± 0.05	7.846ArgArg: 7.846 ± 0.103	4.198ArgSer: 4.198 ± 0.055	4.172ArgThr: 4.172 ± 0.061	5.745ArgVal: 5.745 ± 0.058	1.504ArgTrp: 1.504 ± 0.035	2.593ArgTyr: 2.593 ± 0.043	0.0ArgXaa: 0.0 ± 0.0
Ser	5.494SerAla: 5.494 ± 0.067	0.398SerCys: 0.398 ± 0.017	2.902SerAsp: 2.902 ± 0.043	2.252SerGlu: 2.252 ± 0.038	1.855SerPhe: 1.855 ± 0.037	4.865SerGly: 4.865 ± 0.093	0.951SerHis: 0.951 ± 0.027	2.572SerIle: 2.572 ± 0.048	0.81SerLys: 0.81 ± 0.025	5.066SerLeu: 5.066 ± 0.062	1.068SerMet: 1.068 ± 0.026	1.25SerAsn: 1.25 ± 0.037	3.263SerPro: 3.263 ± 0.06	1.416SerGln: 1.416 ± 0.027	3.224SerArg: 3.224 ± 0.048	2.846SerSer: 2.846 ± 0.058	2.527SerThr: 2.527 ± 0.049	3.67SerVal: 3.67 ± 0.056	0.763SerTrp: 0.763 ± 0.024	1.13SerTyr: 1.13 ± 0.03	0.0SerXaa: 0.0 ± 0.0
Thr	6.121ThrAla: 6.121 ± 0.079	0.493ThrCys: 0.493 ± 0.021	2.894ThrAsp: 2.894 ± 0.051	2.255ThrGlu: 2.255 ± 0.041	2.222ThrPhe: 2.222 ± 0.047	4.819ThrGly: 4.819 ± 0.07	1.115ThrHis: 1.115 ± 0.025	3.762ThrIle: 3.762 ± 0.063	0.733ThrLys: 0.733 ± 0.025	7.266ThrLeu: 7.266 ± 0.096	1.062ThrMet: 1.062 ± 0.026	1.408ThrAsn: 1.408 ± 0.049	4.828ThrPro: 4.828 ± 0.119	1.512ThrGln: 1.512 ± 0.032	3.754ThrArg: 3.754 ± 0.056	2.615ThrSer: 2.615 ± 0.045	3.496ThrThr: 3.496 ± 0.078	4.691ThrVal: 4.691 ± 0.082	0.764ThrTrp: 0.764 ± 0.027	1.418ThrTyr: 1.418 ± 0.042	0.0ThrXaa: 0.0 ± 0.0
Val	9.492ValAla: 9.492 ± 0.1	0.745ValCys: 0.745 ± 0.021	3.847ValAsp: 3.847 ± 0.05	4.593ValGlu: 4.593 ± 0.06	2.531ValPhe: 2.531 ± 0.046	5.138ValGly: 5.138 ± 0.061	1.562ValHis: 1.562 ± 0.034	4.552ValIle: 4.552 ± 0.055	1.301ValLys: 1.301 ± 0.032	7.648ValLeu: 7.648 ± 0.079	1.546ValMet: 1.546 ± 0.031	1.93ValAsn: 1.93 ± 0.044	3.853ValPro: 3.853 ± 0.056	2.744ValGln: 2.744 ± 0.046	6.378ValArg: 6.378 ± 0.062	3.826ValSer: 3.826 ± 0.061	4.746ValThr: 4.746 ± 0.1	6.48ValVal: 6.48 ± 0.069	1.159ValTrp: 1.159 ± 0.029	2.112ValTyr: 2.112 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	1.208TrpAla: 1.208 ± 0.029	0.135TrpCys: 0.135 ± 0.009	0.752TrpAsp: 0.752 ± 0.022	0.839TrpGlu: 0.839 ± 0.028	0.573TrpPhe: 0.573 ± 0.021	1.045TrpGly: 1.045 ± 0.03	0.373TrpHis: 0.373 ± 0.016	0.859TrpIle: 0.859 ± 0.023	0.365TrpLys: 0.365 ± 0.017	1.985TrpLeu: 1.985 ± 0.041	0.436TrpMet: 0.436 ± 0.018	0.566TrpAsn: 0.566 ± 0.02	0.517TrpPro: 0.517 ± 0.019	0.752TrpGln: 0.752 ± 0.021	1.624TrpArg: 1.624 ± 0.034	0.911TrpSer: 0.911 ± 0.026	0.8TrpThr: 0.8 ± 0.022	0.985TrpVal: 0.985 ± 0.026	0.311TrpTrp: 0.311 ± 0.014	0.419TrpTyr: 0.419 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.08TyrAla: 3.08 ± 0.052	0.266TyrCys: 0.266 ± 0.014	1.604TyrAsp: 1.604 ± 0.03	1.485TyrGlu: 1.485 ± 0.027	0.922TyrPhe: 0.922 ± 0.024	2.241TyrGly: 2.241 ± 0.041	0.7TyrHis: 0.7 ± 0.022	1.169TyrIle: 1.169 ± 0.029	0.42TyrLys: 0.42 ± 0.017	2.833TyrLeu: 2.833 ± 0.047	0.419TyrMet: 0.419 ± 0.016	0.719TyrAsn: 0.719 ± 0.02	1.373TyrPro: 1.373 ± 0.039	0.969TyrGln: 0.969 ± 0.028	2.582TyrArg: 2.582 ± 0.044	1.06TyrSer: 1.06 ± 0.028	1.5TyrThr: 1.5 ± 0.036	1.918TyrVal: 1.918 ± 0.036	0.444TyrTrp: 0.444 ± 0.017	0.761TyrTyr: 0.761 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4494 proteins (1633847 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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