Natrialba asiatica (strain ATCC 700177 / DSM 12278 / JCM 9576 / FERM P-10747 / NBRC 102637 / 172P1)
Average proteome isoelectric point is 4.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4175 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M0B519|M0B519_NATA1 Uncharacterized protein OS=Natrialba asiatica (strain ATCC 700177 / DSM 12278 / JCM 9576 / FERM P-10747 / NBRC 102637 / 172P1) OX=29540 GN=C481_00200 PE=4 SV=1
MM1 pKa = 7.64 IYY3 pKa = 10.66 AVTADD8 pKa = 3.35 TGEE11 pKa = 4.02 EE12 pKa = 3.44 RR13 pKa = 11.84 WRR15 pKa = 11.84 FEE17 pKa = 3.71 TNDD20 pKa = 3.41 AVRR23 pKa = 11.84 STPAVVDD30 pKa = 3.47 GTLYY34 pKa = 11.0 VGLHH38 pKa = 6.09 DD39 pKa = 5.71 QMLALDD45 pKa = 4.28 AASGSEE51 pKa = 3.74 QWRR54 pKa = 11.84 VDD56 pKa = 3.62 VAPGGSNPTVVQGEE70 pKa = 4.42 SVYY73 pKa = 10.91 VCRR76 pKa = 11.84 QGAAFGIGGIAKK88 pKa = 9.79 LDD90 pKa = 3.7 SSNGDD95 pKa = 3.55 VQSKK99 pKa = 9.44 FATDD103 pKa = 3.31 TTGGLTAAAVGDD115 pKa = 3.47 QTVYY119 pKa = 10.67 VGSEE123 pKa = 3.99 EE124 pKa = 4.6 GSLSAYY130 pKa = 10.28 DD131 pKa = 4.1 IEE133 pKa = 4.66 TGDD136 pKa = 4.13 VKK138 pKa = 10.52 WSSDD142 pKa = 3.58 VEE144 pKa = 4.29 LGLVGPPALADD155 pKa = 3.37 EE156 pKa = 4.57 TVYY159 pKa = 11.11 VVDD162 pKa = 4.12 IDD164 pKa = 3.59 ATLRR168 pKa = 11.84 AFDD171 pKa = 4.68 PSDD174 pKa = 3.64 GTIHH178 pKa = 7.01 WAEE181 pKa = 3.89 QLPGVSEE188 pKa = 4.36 GSPAVIGGAVYY199 pKa = 10.7 LGDD202 pKa = 3.66 TQGTVHH208 pKa = 7.22 CLATDD213 pKa = 3.68 DD214 pKa = 6.16 GEE216 pKa = 4.77 RR217 pKa = 11.84 LWQADD222 pKa = 3.59 TGNWITPSPAVTDD235 pKa = 3.57 TAVYY239 pKa = 10.22 VCNNDD244 pKa = 3.22 GAVYY248 pKa = 10.22 AIDD251 pKa = 3.51 QTEE254 pKa = 4.94 GEE256 pKa = 4.67 ILWQFATGGQLRR268 pKa = 11.84 TSPAVVDD275 pKa = 3.88 GTAFVGSDD283 pKa = 3.69 DD284 pKa = 3.7 GSLYY288 pKa = 11.21 ALAEE292 pKa = 4.36 DD293 pKa = 4.13 SS294 pKa = 3.86
Molecular weight: 30.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.69
IPC2_protein 3.668
IPC_protein 3.694
Toseland 3.465
ProMoST 3.859
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.63
Rodwell 3.516
Grimsley 3.376
Solomon 3.681
Lehninger 3.643
Nozaki 3.808
DTASelect 4.062
Thurlkill 3.528
EMBOSS 3.643
Sillero 3.821
Patrickios 1.163
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.733
Protein with the highest isoelectric point:
>tr|M0B2W1|M0B2W1_NATA1 Uncharacterized protein OS=Natrialba asiatica (strain ATCC 700177 / DSM 12278 / JCM 9576 / FERM P-10747 / NBRC 102637 / 172P1) OX=29540 GN=C481_03032 PE=4 SV=1
MM1 pKa = 7.42 TCFDD5 pKa = 4.15 DD6 pKa = 4.78 VLTKK10 pKa = 10.66 RR11 pKa = 11.84 LICNVRR17 pKa = 11.84 MARR20 pKa = 11.84 SHH22 pKa = 6.3 LLKK25 pKa = 9.76 TVYY28 pKa = 9.09 MANLRR33 pKa = 11.84 LQLEE37 pKa = 3.96 QRR39 pKa = 11.84 PIGWISFYY47 pKa = 11.33 LSINKK52 pKa = 9.46 LMIGGFVRR60 pKa = 11.84 SQTALQRR67 pKa = 11.84 TT68 pKa = 4.08
Molecular weight: 7.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.736
IPC_protein 10.613
Toseland 10.672
ProMoST 10.394
Dawson 10.774
Bjellqvist 10.511
Wikipedia 10.994
Rodwell 10.965
Grimsley 10.833
Solomon 10.906
Lehninger 10.877
Nozaki 10.672
DTASelect 10.511
Thurlkill 10.672
EMBOSS 11.067
Sillero 10.716
Patrickios 10.774
IPC_peptide 10.906
IPC2_peptide 9.765
IPC2.peptide.svr19 8.387
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4175
0
4175
1209462
26
1675
289.7
31.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.68 ± 0.054
0.739 ± 0.012
8.465 ± 0.048
8.67 ± 0.055
3.309 ± 0.026
8.333 ± 0.04
2.008 ± 0.02
4.561 ± 0.033
1.682 ± 0.022
8.794 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.727 ± 0.015
2.5 ± 0.019
4.667 ± 0.025
2.598 ± 0.025
6.348 ± 0.041
5.801 ± 0.032
7.007 ± 0.033
8.308 ± 0.035
1.134 ± 0.015
2.668 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here