bacterium I07

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1024 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A202DW17|A0A202DW17_9BACT B12-binding domain-containing protein OS=bacterium I07 OX=1932703 GN=BVY01_00640 PE=4 SV=1
MM1 pKa = 7.47RR2 pKa = 11.84RR3 pKa = 11.84HH4 pKa = 5.82MGLILFLFVPGLLDD18 pKa = 3.53AQYY21 pKa = 11.27GNPSLPYY28 pKa = 9.14GQGAYY33 pKa = 10.15GIQTDD38 pKa = 3.74AEE40 pKa = 4.15EE41 pKa = 5.12AEE43 pKa = 4.44EE44 pKa = 4.69GGEE47 pKa = 3.83QAYY50 pKa = 8.85TGWSEE55 pKa = 4.4SKK57 pKa = 9.47TVTITGSDD65 pKa = 3.32SGDD68 pKa = 3.2ATGYY72 pKa = 11.6ALFLDD77 pKa = 3.8IDD79 pKa = 4.26YY80 pKa = 8.38ATGMDD85 pKa = 3.81SLFRR89 pKa = 11.84DD90 pKa = 3.7LRR92 pKa = 11.84FSQDD96 pKa = 2.7GEE98 pKa = 4.13ALHH101 pKa = 6.75FWRR104 pKa = 11.84EE105 pKa = 4.18SVFKK109 pKa = 10.81SDD111 pKa = 5.3SALFWVKK118 pKa = 10.37VPSIPLGDD126 pKa = 4.11DD127 pKa = 3.37VTVTLHH133 pKa = 6.06YY134 pKa = 11.45SNDD137 pKa = 3.21TCATDD142 pKa = 3.65ISNGDD147 pKa = 3.35STFAFFDD154 pKa = 3.8DD155 pKa = 5.39FGDD158 pKa = 3.72EE159 pKa = 4.84SIDD162 pKa = 3.69SEE164 pKa = 4.22KK165 pKa = 10.58WPTSLEE171 pKa = 3.93LGQFTEE177 pKa = 4.13NANGYY182 pKa = 10.39LEE184 pKa = 4.46VNHH187 pKa = 6.72TGMITMLLQATNDD200 pKa = 3.84TVHH203 pKa = 6.83VPIAWKK209 pKa = 8.83TRR211 pKa = 11.84INQGTANLANYY222 pKa = 8.73RR223 pKa = 11.84RR224 pKa = 11.84EE225 pKa = 4.43GIGVSTGYY233 pKa = 10.91DD234 pKa = 3.12LGTTQAGTAAYY245 pKa = 9.9YY246 pKa = 10.39YY247 pKa = 10.74NHH249 pKa = 7.85DD250 pKa = 3.93DD251 pKa = 3.61ATWRR255 pKa = 11.84SDD257 pKa = 2.9EE258 pKa = 4.34STSLLAQTDD267 pKa = 4.07WVPDD271 pKa = 3.24ITFDD275 pKa = 3.18QWVVLEE281 pKa = 4.93GKK283 pKa = 10.46ASVDD287 pKa = 2.75SCYY290 pKa = 10.35FYY292 pKa = 11.2QNQAYY297 pKa = 9.99DD298 pKa = 3.73LTHH301 pKa = 7.57DD302 pKa = 4.14YY303 pKa = 11.2SPQNDD308 pKa = 3.61ADD310 pKa = 5.07LIPP313 pKa = 4.63

Molecular weight: 34.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A202DT02|A0A202DT02_9BACT Uncharacterized protein OS=bacterium I07 OX=1932703 GN=BVY01_03535 PE=4 SV=1
MM1 pKa = 8.08LIRR4 pKa = 11.84SATSLSKK11 pKa = 10.8NSRR14 pKa = 11.84IRR16 pKa = 11.84NDD18 pKa = 2.8IFEE21 pKa = 5.73LIVTLLLTLTLLVRR35 pKa = 11.84MLVLGYY41 pKa = 10.18VSAGFSMDD49 pKa = 3.13MLGRR53 pKa = 11.84ICSFAWIEE61 pKa = 3.94KK62 pKa = 9.76QIYY65 pKa = 9.56CISCQRR71 pKa = 11.84MAIVNDD77 pKa = 4.86FGALRR82 pKa = 11.84PRR84 pKa = 11.84TQLAQLKK91 pKa = 9.76LFAFGRR97 pKa = 11.84KK98 pKa = 7.98AA99 pKa = 3.08

Molecular weight: 11.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1024

0

1024

315021

27

1666

307.6

34.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.33 ± 0.072

1.053 ± 0.027

5.852 ± 0.065

6.392 ± 0.061

4.471 ± 0.054

7.23 ± 0.08

2.265 ± 0.031

7.464 ± 0.074

6.063 ± 0.082

9.228 ± 0.099

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.492 ± 0.03

4.554 ± 0.064

4.272 ± 0.055

3.541 ± 0.041

5.415 ± 0.061

6.668 ± 0.065

5.166 ± 0.048

6.37 ± 0.065

1.535 ± 0.036

3.638 ± 0.059
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski