Halorubrum sp. Ib24

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Halorubraceae; Halorubrum; unclassified Halorubrum

Average proteome isoelectric point is 4.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2609 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A256H947|A0A256H947_9EURY Branched-chain amino acid ABC transporter permease OS=Halorubrum sp. Ib24 OX=1383850 GN=DJ82_06830 PE=3 SV=1
MM1 pKa = 7.68SDD3 pKa = 3.35IEE5 pKa = 4.32TSTVSEE11 pKa = 4.09EE12 pKa = 5.04GYY14 pKa = 10.95ACTSQVGDD22 pKa = 4.02FDD24 pKa = 4.52LQIDD28 pKa = 4.09ATDD31 pKa = 3.52EE32 pKa = 4.15TGPNPNAALVATYY45 pKa = 10.17ASCYY49 pKa = 10.27LPAFRR54 pKa = 11.84VGGSQRR60 pKa = 11.84GEE62 pKa = 3.73EE63 pKa = 4.27DD64 pKa = 3.05LGKK67 pKa = 10.15IQIDD71 pKa = 3.73ASADD75 pKa = 3.68LDD77 pKa = 5.13DD78 pKa = 6.67DD79 pKa = 5.59DD80 pKa = 6.54DD81 pKa = 4.43LAAVAFDD88 pKa = 3.43VHH90 pKa = 7.86VEE92 pKa = 3.79ADD94 pKa = 4.23LDD96 pKa = 4.18DD97 pKa = 4.16EE98 pKa = 4.59TASDD102 pKa = 3.17IAEE105 pKa = 4.08RR106 pKa = 11.84AEE108 pKa = 5.0GICHH112 pKa = 4.59VHH114 pKa = 5.25SALRR118 pKa = 11.84EE119 pKa = 4.03GLHH122 pKa = 6.8ADD124 pKa = 3.23VSVYY128 pKa = 10.47PGAFF132 pKa = 3.1

Molecular weight: 13.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A256HFU7|A0A256HFU7_9EURY DNA polymerase IV OS=Halorubrum sp. Ib24 OX=1383850 GN=dbh PE=3 SV=1
MM1 pKa = 7.32HH2 pKa = 6.78GVEE5 pKa = 3.61RR6 pKa = 11.84RR7 pKa = 11.84ARR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84GRR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84SRR22 pKa = 11.84SGGVSRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84GRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84VRR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84AGRR44 pKa = 11.84DD45 pKa = 2.72RR46 pKa = 11.84AARR49 pKa = 11.84LPDD52 pKa = 3.69PRR54 pKa = 11.84DD55 pKa = 3.85AGDD58 pKa = 3.81HH59 pKa = 6.39APVLPRR65 pKa = 11.84PGPRR69 pKa = 11.84RR70 pKa = 11.84VRR72 pKa = 3.4

Molecular weight: 8.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2609

0

2609

761550

35

2446

291.9

31.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.82 ± 0.084

0.663 ± 0.013

8.793 ± 0.068

8.388 ± 0.063

3.274 ± 0.034

9.158 ± 0.05

1.88 ± 0.024

3.761 ± 0.035

1.644 ± 0.027

8.587 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.627 ± 0.02

2.191 ± 0.032

4.765 ± 0.031

2.066 ± 0.039

6.871 ± 0.062

5.427 ± 0.038

6.166 ± 0.042

9.297 ± 0.054

1.076 ± 0.019

2.547 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski