Amino acid dipepetide frequency for Halorubrum sp. Ib24

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.292AlaAla: 16.292 ± 0.238
0.722AlaCys: 0.722 ± 0.036
11.026AlaAsp: 11.026 ± 0.163
9.544AlaGlu: 9.544 ± 0.139
4.441AlaPhe: 4.441 ± 0.089
11.168AlaGly: 11.168 ± 0.155
1.788AlaHis: 1.788 ± 0.054
4.735AlaIle: 4.735 ± 0.089
1.708AlaLys: 1.708 ± 0.049
10.782AlaLeu: 10.782 ± 0.145
2.005AlaMet: 2.005 ± 0.05
2.343AlaAsn: 2.343 ± 0.06
4.312AlaPro: 4.312 ± 0.086
1.841AlaGln: 1.841 ± 0.062
6.445AlaArg: 6.445 ± 0.113
5.691AlaSer: 5.691 ± 0.101
7.31AlaThr: 7.31 ± 0.113
12.2AlaVal: 12.2 ± 0.179
1.116AlaTrp: 1.116 ± 0.038
2.726AlaTyr: 2.726 ± 0.061
0.0AlaXaa: 0.0 ± 0.0
Cys
0.604CysAla: 0.604 ± 0.033
0.071CysCys: 0.071 ± 0.01
0.559CysAsp: 0.559 ± 0.036
0.513CysGlu: 0.513 ± 0.029
0.192CysPhe: 0.192 ± 0.017
0.855CysGly: 0.855 ± 0.039
0.185CysHis: 0.185 ± 0.016
0.194CysIle: 0.194 ± 0.017
0.102CysLys: 0.102 ± 0.012
0.513CysLeu: 0.513 ± 0.024
0.076CysMet: 0.076 ± 0.011
0.171CysAsn: 0.171 ± 0.016
0.506CysPro: 0.506 ± 0.03
0.135CysGln: 0.135 ± 0.014
0.475CysArg: 0.475 ± 0.027
0.382CysSer: 0.382 ± 0.02
0.39CysThr: 0.39 ± 0.026
0.477CysVal: 0.477 ± 0.027
0.063CysTrp: 0.063 ± 0.009
0.169CysTyr: 0.169 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
12.091AspAla: 12.091 ± 0.17
0.548AspCys: 0.548 ± 0.028
8.35AspAsp: 8.35 ± 0.147
8.225AspGlu: 8.225 ± 0.15
1.909AspPhe: 1.909 ± 0.049
9.812AspGly: 9.812 ± 0.157
1.752AspHis: 1.752 ± 0.052
2.53AspIle: 2.53 ± 0.06
0.852AspLys: 0.852 ± 0.039
7.083AspLeu: 7.083 ± 0.11
1.211AspMet: 1.211 ± 0.036
1.19AspAsn: 1.19 ± 0.051
5.285AspPro: 5.285 ± 0.092
1.484AspGln: 1.484 ± 0.089
7.588AspArg: 7.588 ± 0.126
3.456AspSer: 3.456 ± 0.074
3.777AspThr: 3.777 ± 0.092
8.15AspVal: 8.15 ± 0.112
0.945AspTrp: 0.945 ± 0.035
1.69AspTyr: 1.69 ± 0.051
0.0AspXaa: 0.0 ± 0.0
Glu
9.401GluAla: 9.401 ± 0.148
0.5GluCys: 0.5 ± 0.034
4.899GluAsp: 4.899 ± 0.106
6.449GluGlu: 6.449 ± 0.143
2.905GluPhe: 2.905 ± 0.065
5.965GluGly: 5.965 ± 0.105
1.845GluHis: 1.845 ± 0.054
3.606GluIle: 3.606 ± 0.095
1.643GluLys: 1.643 ± 0.06
7.411GluLeu: 7.411 ± 0.144
1.834GluMet: 1.834 ± 0.059
2.027GluAsn: 2.027 ± 0.062
4.206GluPro: 4.206 ± 0.081
2.013GluGln: 2.013 ± 0.074
7.764GluArg: 7.764 ± 0.129
5.453GluSer: 5.453 ± 0.089
6.416GluThr: 6.416 ± 0.097
5.984GluVal: 5.984 ± 0.091
1.082GluTrp: 1.082 ± 0.045
2.475GluTyr: 2.475 ± 0.075
0.0GluXaa: 0.0 ± 0.0
Phe
3.952PheAla: 3.952 ± 0.085
0.243PheCys: 0.243 ± 0.018
3.402PheAsp: 3.402 ± 0.076
3.081PheGlu: 3.081 ± 0.063
1.157PhePhe: 1.157 ± 0.154
3.309PheGly: 3.309 ± 0.076
0.586PheHis: 0.586 ± 0.03
0.985PheIle: 0.985 ± 0.04
0.408PheLys: 0.408 ± 0.021
2.801PheLeu: 2.801 ± 0.079
0.457PheMet: 0.457 ± 0.026
0.716PheAsn: 0.716 ± 0.032
1.322PhePro: 1.322 ± 0.043
0.693PheGln: 0.693 ± 0.027
1.863PheArg: 1.863 ± 0.048
1.58PheSer: 1.58 ± 0.043
1.828PheThr: 1.828 ± 0.047
3.201PheVal: 3.201 ± 0.078
0.344PheTrp: 0.344 ± 0.024
0.808PheTyr: 0.808 ± 0.03
0.0PheXaa: 0.0 ± 0.0
Gly
9.511GlyAla: 9.511 ± 0.16
0.7GlyCys: 0.7 ± 0.032
8.539GlyAsp: 8.539 ± 0.128
7.617GlyGlu: 7.617 ± 0.12
3.284GlyPhe: 3.284 ± 0.075
8.647GlyGly: 8.647 ± 0.169
1.67GlyHis: 1.67 ± 0.052
4.153GlyIle: 4.153 ± 0.075
1.737GlyLys: 1.737 ± 0.055
7.426GlyLeu: 7.426 ± 0.135
1.593GlyMet: 1.593 ± 0.053
1.972GlyAsn: 1.972 ± 0.068
3.723GlyPro: 3.723 ± 0.076
1.729GlyGln: 1.729 ± 0.059
5.532GlyArg: 5.532 ± 0.104
5.531GlySer: 5.531 ± 0.097
5.807GlyThr: 5.807 ± 0.096
8.554GlyVal: 8.554 ± 0.115
1.114GlyTrp: 1.114 ± 0.043
2.743GlyTyr: 2.743 ± 0.057
0.0GlyXaa: 0.0 ± 0.0
His
2.102HisAla: 2.102 ± 0.057
0.19HisCys: 0.19 ± 0.014
1.698HisAsp: 1.698 ± 0.049
1.555HisGlu: 1.555 ± 0.046
0.473HisPhe: 0.473 ± 0.025
1.979HisGly: 1.979 ± 0.047
0.478HisHis: 0.478 ± 0.026
0.49HisIle: 0.49 ± 0.028
0.227HisLys: 0.227 ± 0.02
1.534HisLeu: 1.534 ± 0.051
0.267HisMet: 0.267 ± 0.018
0.435HisAsn: 0.435 ± 0.026
1.216HisPro: 1.216 ± 0.041
0.424HisGln: 0.424 ± 0.025
1.309HisArg: 1.309 ± 0.043
0.804HisSer: 0.804 ± 0.036
1.028HisThr: 1.028 ± 0.042
1.883HisVal: 1.883 ± 0.052
0.21HisTrp: 0.21 ± 0.018
0.496HisTyr: 0.496 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
4.857IleAla: 4.857 ± 0.081
0.251IleCys: 0.251 ± 0.019
3.916IleAsp: 3.916 ± 0.093
3.994IleGlu: 3.994 ± 0.08
0.741IlePhe: 0.741 ± 0.035
3.879IleGly: 3.879 ± 0.086
0.734IleHis: 0.734 ± 0.029
1.062IleIle: 1.062 ± 0.043
0.653IleLys: 0.653 ± 0.033
2.549IleLeu: 2.549 ± 0.064
0.422IleMet: 0.422 ± 0.026
0.871IleAsn: 0.871 ± 0.036
1.899IlePro: 1.899 ± 0.044
0.939IleGln: 0.939 ± 0.038
2.544IleArg: 2.544 ± 0.062
1.854IleSer: 1.854 ± 0.05
2.046IleThr: 2.046 ± 0.055
3.388IleVal: 3.388 ± 0.075
0.26IleTrp: 0.26 ± 0.018
0.755IleTyr: 0.755 ± 0.035
0.0IleXaa: 0.0 ± 0.0
Lys
1.662LysAla: 1.662 ± 0.049
0.101LysCys: 0.101 ± 0.011
0.835LysAsp: 0.835 ± 0.041
1.254LysGlu: 1.254 ± 0.054
0.477LysPhe: 0.477 ± 0.024
1.295LysGly: 1.295 ± 0.045
0.404LysHis: 0.404 ± 0.024
0.645LysIle: 0.645 ± 0.032
0.44LysLys: 0.44 ± 0.028
1.456LysLeu: 1.456 ± 0.048
0.32LysMet: 0.32 ± 0.023
0.461LysAsn: 0.461 ± 0.027
0.843LysPro: 0.843 ± 0.033
0.679LysGln: 0.679 ± 0.034
1.607LysArg: 1.607 ± 0.051
1.006LysSer: 1.006 ± 0.045
1.124LysThr: 1.124 ± 0.047
1.094LysVal: 1.094 ± 0.048
0.24LysTrp: 0.24 ± 0.017
0.494LysTyr: 0.494 ± 0.028
0.0LysXaa: 0.0 ± 0.0
Leu
11.159LeuAla: 11.159 ± 0.146
0.517LeuCys: 0.517 ± 0.028
7.708LeuAsp: 7.708 ± 0.135
5.594LeuGlu: 5.594 ± 0.108
2.997LeuPhe: 2.997 ± 0.08
7.552LeuGly: 7.552 ± 0.136
1.347LeuHis: 1.347 ± 0.043
3.052LeuIle: 3.052 ± 0.063
1.472LeuLys: 1.472 ± 0.06
8.21LeuLeu: 8.21 ± 0.151
1.251LeuMet: 1.251 ± 0.043
1.735LeuAsn: 1.735 ± 0.05
4.181LeuPro: 4.181 ± 0.063
1.658LeuGln: 1.658 ± 0.047
5.948LeuArg: 5.948 ± 0.1
5.511LeuSer: 5.511 ± 0.093
5.048LeuThr: 5.048 ± 0.096
8.042LeuVal: 8.042 ± 0.129
0.835LeuTrp: 0.835 ± 0.037
2.052LeuTyr: 2.052 ± 0.051
0.0LeuXaa: 0.0 ± 0.0
Met
1.796MetAla: 1.796 ± 0.051
0.102MetCys: 0.102 ± 0.013
1.148MetAsp: 1.148 ± 0.042
1.09MetGlu: 1.09 ± 0.04
0.475MetPhe: 0.475 ± 0.028
1.267MetGly: 1.267 ± 0.039
0.288MetHis: 0.288 ± 0.019
0.649MetIle: 0.649 ± 0.031
0.394MetLys: 0.394 ± 0.024
1.338MetLeu: 1.338 ± 0.045
0.257MetMet: 0.257 ± 0.017
0.586MetAsn: 0.586 ± 0.026
0.746MetPro: 0.746 ± 0.032
0.376MetGln: 0.376 ± 0.022
1.056MetArg: 1.056 ± 0.036
1.519MetSer: 1.519 ± 0.047
1.465MetThr: 1.465 ± 0.042
1.145MetVal: 1.145 ± 0.043
0.143MetTrp: 0.143 ± 0.014
0.431MetTyr: 0.431 ± 0.023
0.0MetXaa: 0.0 ± 0.0
Asn
2.692AsnAla: 2.692 ± 0.068
0.173AsnCys: 0.173 ± 0.014
1.657AsnAsp: 1.657 ± 0.053
1.736AsnGlu: 1.736 ± 0.051
0.622AsnPhe: 0.622 ± 0.032
2.067AsnGly: 2.067 ± 0.062
0.452AsnHis: 0.452 ± 0.028
0.688AsnIle: 0.688 ± 0.031
0.378AsnLys: 0.378 ± 0.023
1.708AsnLeu: 1.708 ± 0.048
0.349AsnMet: 0.349 ± 0.019
0.491AsnAsn: 0.491 ± 0.027
1.448AsnPro: 1.448 ± 0.044
0.515AsnGln: 0.515 ± 0.028
1.581AsnArg: 1.581 ± 0.05
0.951AsnSer: 0.951 ± 0.041
1.224AsnThr: 1.224 ± 0.043
2.232AsnVal: 2.232 ± 0.054
0.31AsnTrp: 0.31 ± 0.019
0.633AsnTyr: 0.633 ± 0.036
0.0AsnXaa: 0.0 ± 0.0
Pro
5.09ProAla: 5.09 ± 0.095
0.232ProCys: 0.232 ± 0.018
5.255ProAsp: 5.255 ± 0.093
4.621ProGlu: 4.621 ± 0.085
1.653ProPhe: 1.653 ± 0.047
4.103ProGly: 4.103 ± 0.081
0.884ProHis: 0.884 ± 0.033
1.879ProIle: 1.879 ± 0.055
0.8ProLys: 0.8 ± 0.034
3.591ProLeu: 3.591 ± 0.076
0.815ProMet: 0.815 ± 0.032
1.125ProAsn: 1.125 ± 0.042
2.281ProPro: 2.281 ± 0.061
0.81ProGln: 0.81 ± 0.032
2.398ProArg: 2.398 ± 0.055
2.789ProSer: 2.789 ± 0.063
3.281ProThr: 3.281 ± 0.065
4.392ProVal: 4.392 ± 0.095
0.483ProTrp: 0.483 ± 0.025
1.169ProTyr: 1.169 ± 0.04
0.0ProXaa: 0.0 ± 0.0
Gln
1.979GlnAla: 1.979 ± 0.059
0.131GlnCys: 0.131 ± 0.013
1.103GlnAsp: 1.103 ± 0.087
1.483GlnGlu: 1.483 ± 0.061
0.812GlnPhe: 0.812 ± 0.032
1.434GlnGly: 1.434 ± 0.05
0.432GlnHis: 0.432 ± 0.024
0.994GlnIle: 0.994 ± 0.04
0.495GlnLys: 0.495 ± 0.031
1.951GlnLeu: 1.951 ± 0.057
0.418GlnMet: 0.418 ± 0.023
0.628GlnAsn: 0.628 ± 0.035
0.913GlnPro: 0.913 ± 0.034
0.8GlnGln: 0.8 ± 0.047
1.75GlnArg: 1.75 ± 0.055
1.442GlnSer: 1.442 ± 0.087
1.366GlnThr: 1.366 ± 0.064
1.511GlnVal: 1.511 ± 0.047
0.305GlnTrp: 0.305 ± 0.02
0.717GlnTyr: 0.717 ± 0.031
0.0GlnXaa: 0.0 ± 0.0
Arg
6.789ArgAla: 6.789 ± 0.112
0.479ArgCys: 0.479 ± 0.03
5.606ArgAsp: 5.606 ± 0.103
6.864ArgGlu: 6.864 ± 0.117
2.448ArgPhe: 2.448 ± 0.065
5.008ArgGly: 5.008 ± 0.107
1.27ArgHis: 1.27 ± 0.042
3.067ArgIle: 3.067 ± 0.071
1.274ArgLys: 1.274 ± 0.04
6.408ArgLeu: 6.408 ± 0.105
1.305ArgMet: 1.305 ± 0.043
1.727ArgAsn: 1.727 ± 0.049
2.843ArgPro: 2.843 ± 0.07
1.568ArgGln: 1.568 ± 0.052
5.478ArgArg: 5.478 ± 0.112
3.855ArgSer: 3.855 ± 0.078
4.021ArgThr: 4.021 ± 0.085
5.931ArgVal: 5.931 ± 0.107
0.792ArgTrp: 0.792 ± 0.033
1.974ArgTyr: 1.974 ± 0.048
0.0ArgXaa: 0.0 ± 0.0
Ser
5.734SerAla: 5.734 ± 0.096
0.319SerCys: 0.319 ± 0.023
4.814SerAsp: 4.814 ± 0.095
4.404SerGlu: 4.404 ± 0.077
1.949SerPhe: 1.949 ± 0.05
5.554SerGly: 5.554 ± 0.087
0.982SerHis: 0.982 ± 0.033
2.228SerIle: 2.228 ± 0.057
1.015SerLys: 1.015 ± 0.042
4.773SerLeu: 4.773 ± 0.088
0.973SerMet: 0.973 ± 0.034
1.289SerAsn: 1.289 ± 0.047
2.638SerPro: 2.638 ± 0.062
1.219SerGln: 1.219 ± 0.061
3.257SerArg: 3.257 ± 0.071
2.645SerSer: 2.645 ± 0.07
3.376SerThr: 3.376 ± 0.075
5.171SerVal: 5.171 ± 0.088
0.575SerTrp: 0.575 ± 0.026
1.351SerTyr: 1.351 ± 0.048
0.0SerXaa: 0.0 ± 0.0
Thr
7.095ThrAla: 7.095 ± 0.117
0.366ThrCys: 0.366 ± 0.023
5.639ThrAsp: 5.639 ± 0.109
4.738ThrGlu: 4.738 ± 0.08
1.979ThrPhe: 1.979 ± 0.055
6.06ThrGly: 6.06 ± 0.082
1.141ThrHis: 1.141 ± 0.042
2.31ThrIle: 2.31 ± 0.059
0.922ThrLys: 0.922 ± 0.041
5.401ThrLeu: 5.401 ± 0.086
0.877ThrMet: 0.877 ± 0.039
1.314ThrAsn: 1.314 ± 0.042
3.187ThrPro: 3.187 ± 0.068
1.179ThrGln: 1.179 ± 0.06
3.411ThrArg: 3.411 ± 0.075
2.811ThrSer: 2.811 ± 0.071
3.937ThrThr: 3.937 ± 0.093
7.088ThrVal: 7.088 ± 0.145
0.594ThrTrp: 0.594 ± 0.033
1.61ThrTyr: 1.61 ± 0.045
0.0ThrXaa: 0.0 ± 0.0
Val
11.548ValAla: 11.548 ± 0.176
0.684ValCys: 0.684 ± 0.033
8.56ValAsp: 8.56 ± 0.135
7.908ValGlu: 7.908 ± 0.122
2.962ValPhe: 2.962 ± 0.067
8.529ValGly: 8.529 ± 0.143
1.7ValHis: 1.7 ± 0.056
3.232ValIle: 3.232 ± 0.063
1.275ValLys: 1.275 ± 0.043
7.483ValLeu: 7.483 ± 0.126
1.282ValMet: 1.282 ± 0.047
1.968ValAsn: 1.968 ± 0.047
4.438ValPro: 4.438 ± 0.081
1.631ValGln: 1.631 ± 0.042
5.951ValArg: 5.951 ± 0.116
5.301ValSer: 5.301 ± 0.096
6.144ValThr: 6.144 ± 0.109
9.402ValVal: 9.402 ± 0.16
0.875ValTrp: 0.875 ± 0.035
2.093ValTyr: 2.093 ± 0.055
0.0ValXaa: 0.0 ± 0.0
Trp
0.96TrpAla: 0.96 ± 0.03
0.081TrpCys: 0.081 ± 0.01
0.734TrpAsp: 0.734 ± 0.035
0.884TrpGlu: 0.884 ± 0.04
0.414TrpPhe: 0.414 ± 0.026
0.805TrpGly: 0.805 ± 0.035
0.21TrpHis: 0.21 ± 0.016
0.499TrpIle: 0.499 ± 0.028
0.243TrpLys: 0.243 ± 0.018
1.149TrpLeu: 1.149 ± 0.046
0.218TrpMet: 0.218 ± 0.018
0.309TrpAsn: 0.309 ± 0.021
0.454TrpPro: 0.454 ± 0.023
0.318TrpGln: 0.318 ± 0.021
0.864TrpArg: 0.864 ± 0.032
0.578TrpSer: 0.578 ± 0.03
0.696TrpThr: 0.696 ± 0.029
0.854TrpVal: 0.854 ± 0.032
0.16TrpTrp: 0.16 ± 0.013
0.336TrpTyr: 0.336 ± 0.024
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.882TyrAla: 2.882 ± 0.074
0.221TyrCys: 0.221 ± 0.017
2.48TyrAsp: 2.48 ± 0.062
2.327TyrGlu: 2.327 ± 0.059
0.848TyrPhe: 0.848 ± 0.032
2.294TyrGly: 2.294 ± 0.058
0.649TyrHis: 0.649 ± 0.032
0.615TyrIle: 0.615 ± 0.027
0.399TyrLys: 0.399 ± 0.023
2.301TyrLeu: 2.301 ± 0.05
0.339TyrMet: 0.339 ± 0.02
0.552TyrAsn: 0.552 ± 0.031
1.212TyrPro: 1.212 ± 0.041
0.651TyrGln: 0.651 ± 0.026
1.883TyrArg: 1.883 ± 0.056
1.108TyrSer: 1.108 ± 0.04
1.376TyrThr: 1.376 ± 0.045
2.266TyrVal: 2.266 ± 0.061
0.319TyrTrp: 0.319 ± 0.02
0.752TyrTyr: 0.752 ± 0.03
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2609 proteins (761550 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski