Histidinibacterium sp. 176SS1-4

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Histidinibacterium; unclassified Histidinibacterium

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3759 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J5GE64|A0A5J5GE64_9RHOB Cadmium-translocating P-type ATPase OS=Histidinibacterium sp. 176SS1-4 OX=2613962 GN=cadA PE=3 SV=1
MM1 pKa = 7.14TLKK4 pKa = 10.66PLTILGALAAGTVALPASAQEE25 pKa = 4.27YY26 pKa = 10.59CGGLSSGAWIGGNEE40 pKa = 4.14ANSDD44 pKa = 3.26IGSAEE49 pKa = 4.02TFRR52 pKa = 11.84EE53 pKa = 3.82QLALVLLGNAHH64 pKa = 6.44TSLFSLSEE72 pKa = 3.95AASVRR77 pKa = 11.84LEE79 pKa = 3.91AEE81 pKa = 3.85ARR83 pKa = 11.84GQGDD87 pKa = 3.5PVIEE91 pKa = 4.19VLDD94 pKa = 4.06GEE96 pKa = 4.81GGVVATDD103 pKa = 3.87DD104 pKa = 3.95DD105 pKa = 4.44SGGGVSSAALVALEE119 pKa = 4.54PGTYY123 pKa = 10.15CLQTSSYY130 pKa = 11.61DD131 pKa = 3.82NAPLTATVRR140 pKa = 11.84IGRR143 pKa = 11.84AEE145 pKa = 4.01HH146 pKa = 6.08EE147 pKa = 4.27ALTEE151 pKa = 4.32GGATLTAAGPGDD163 pKa = 3.93TTEE166 pKa = 4.4TPAPTGASPVATDD179 pKa = 3.59TEE181 pKa = 4.63ACGGAVMLASGPLDD195 pKa = 4.13DD196 pKa = 5.77SLSQGVSRR204 pKa = 11.84TEE206 pKa = 4.02SIEE209 pKa = 4.06AVPTYY214 pKa = 10.92AFDD217 pKa = 4.17LASPASVSITAEE229 pKa = 4.01NPDD232 pKa = 4.1ADD234 pKa = 4.06PVLTLTGDD242 pKa = 3.57DD243 pKa = 3.51GTFYY247 pKa = 11.35AEE249 pKa = 3.86NDD251 pKa = 4.05DD252 pKa = 4.03YY253 pKa = 11.99DD254 pKa = 4.16GLNSRR259 pKa = 11.84IDD261 pKa = 3.47MTTPLAAGTYY271 pKa = 8.88CVQLRR276 pKa = 11.84ALSDD280 pKa = 3.01ATAPVTVSVSGFDD293 pKa = 3.65PEE295 pKa = 4.55AAAQEE300 pKa = 4.6TYY302 pKa = 11.15NRR304 pKa = 11.84GQAAPPLDD312 pKa = 3.39GSYY315 pKa = 10.78PVTDD319 pKa = 4.61LGTLSGRR326 pKa = 11.84VVTDD330 pKa = 3.32AQIQGEE336 pKa = 4.34TAQWFSVTLDD346 pKa = 3.43APGLLLVEE354 pKa = 5.55AIGAAAGGDD363 pKa = 3.4PVLRR367 pKa = 11.84VFDD370 pKa = 4.04DD371 pKa = 3.43VGRR374 pKa = 11.84EE375 pKa = 3.3IAYY378 pKa = 10.52NDD380 pKa = 5.0DD381 pKa = 3.55YY382 pKa = 11.18GQSYY386 pKa = 10.59DD387 pKa = 3.47SQIAARR393 pKa = 11.84TFPGTYY399 pKa = 10.24LIALTDD405 pKa = 3.85ISATSPLLRR414 pKa = 11.84LVIEE418 pKa = 4.7RR419 pKa = 11.84YY420 pKa = 9.97VPAEE424 pKa = 3.71

Molecular weight: 43.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J5GR28|A0A5J5GR28_9RHOB Peptidoglycan-associated protein OS=Histidinibacterium sp. 176SS1-4 OX=2613962 GN=pal PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84VRR12 pKa = 11.84KK13 pKa = 8.99NRR15 pKa = 11.84HH16 pKa = 3.77GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.43GGRR28 pKa = 11.84KK29 pKa = 8.99VLNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3759

0

3759

1173173

28

8883

312.1

33.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.304 ± 0.056

0.823 ± 0.015

5.934 ± 0.044

6.965 ± 0.048

3.516 ± 0.03

9.041 ± 0.049

1.946 ± 0.023

4.705 ± 0.03

2.458 ± 0.033

10.258 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.546 ± 0.022

2.153 ± 0.022

5.343 ± 0.041

2.86 ± 0.023

7.492 ± 0.05

5.162 ± 0.031

5.444 ± 0.036

7.465 ± 0.034

1.456 ± 0.017

2.131 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski