Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Metschnikowiaceae; Metschnikowia; Metschnikowia bicuspidata; Metschnikowia bicuspidata var. bicuspidata

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5827 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1A0H6J0|A0A1A0H6J0_9ASCO Putative transferase CAF17 mitochondrial OS=Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993 OX=869754 GN=METBIDRAFT_33194 PE=3 SV=1
MM1 pKa = 7.65RR2 pKa = 11.84LSVLAWVATVLAGYY16 pKa = 10.67APFEE20 pKa = 4.3IDD22 pKa = 3.72CPEE25 pKa = 4.09GNLLRR30 pKa = 11.84LANSLSEE37 pKa = 4.54EE38 pKa = 4.06EE39 pKa = 4.36NDD41 pKa = 3.63WVTEE45 pKa = 3.98RR46 pKa = 11.84HH47 pKa = 5.78KK48 pKa = 11.03VTDD51 pKa = 3.61SYY53 pKa = 11.97LEE55 pKa = 4.13TFLNDD60 pKa = 3.74AMLDD64 pKa = 3.79DD65 pKa = 5.31FDD67 pKa = 6.06ASSFLRR73 pKa = 11.84NTTNNRR79 pKa = 11.84SITIGLAFSGGGYY92 pKa = 9.95RR93 pKa = 11.84AMLAGAGQLSALDD106 pKa = 3.65NRR108 pKa = 11.84TEE110 pKa = 4.07GAHH113 pKa = 6.87AEE115 pKa = 4.18GLGGLLQASTYY126 pKa = 10.9LVGLSGGSWLVGSVANNNFTSIQDD150 pKa = 3.35IVDD153 pKa = 3.57NKK155 pKa = 10.19IDD157 pKa = 3.51IWDD160 pKa = 3.82LEE162 pKa = 4.29YY163 pKa = 11.22SVVNMGGWNPIEE175 pKa = 4.24AYY177 pKa = 9.87QYY179 pKa = 11.61YY180 pKa = 10.29KK181 pKa = 10.95DD182 pKa = 4.93LYY184 pKa = 10.55DD185 pKa = 4.43DD186 pKa = 3.98VSAKK190 pKa = 10.21EE191 pKa = 3.82DD192 pKa = 3.3AGFDD196 pKa = 3.83VSLTDD201 pKa = 2.89TWGRR205 pKa = 11.84ALSHH209 pKa = 6.17QFFTEE214 pKa = 3.8QSDD217 pKa = 3.79YY218 pKa = 11.63GEE220 pKa = 4.62NILWSGIQDD229 pKa = 3.07IPEE232 pKa = 4.37FADD235 pKa = 3.19HH236 pKa = 6.89EE237 pKa = 4.76MPFPIVVADD246 pKa = 3.59GRR248 pKa = 11.84TPGTFVISGNSTVFEE263 pKa = 4.22FNPFEE268 pKa = 4.27MGSWDD273 pKa = 3.38PSLYY277 pKa = 10.95AFTKK281 pKa = 9.48TKK283 pKa = 10.92YY284 pKa = 10.39LGTDD288 pKa = 2.96VSDD291 pKa = 3.85GVPVNGSCIGGFDD304 pKa = 3.14NSGFVMGTSSSLFNQFVLQINTTSLSSTIQSLISLFLEE342 pKa = 5.92DD343 pKa = 5.02ISQDD347 pKa = 3.53EE348 pKa = 4.29NDD350 pKa = 3.33IALYY354 pKa = 10.15KK355 pKa = 10.36PNPFQNFSEE364 pKa = 4.67AGVDD368 pKa = 4.44SIVQNDD374 pKa = 3.74TLYY377 pKa = 11.27LVDD380 pKa = 5.32GGEE383 pKa = 4.33DD384 pKa = 3.43LQNIPLYY391 pKa = 10.11PLLQHH396 pKa = 6.22PRR398 pKa = 11.84EE399 pKa = 3.93VDD401 pKa = 3.57VIFAYY406 pKa = 10.55DD407 pKa = 3.46NSADD411 pKa = 3.94TDD413 pKa = 4.15EE414 pKa = 4.55NWPDD418 pKa = 3.51GASMVASYY426 pKa = 10.61QRR428 pKa = 11.84QFLEE432 pKa = 4.16QGNGTIFPYY441 pKa = 10.79VPDD444 pKa = 3.59VNSFRR449 pKa = 11.84NLNLTAKK456 pKa = 10.03PAFFGCNARR465 pKa = 11.84NLSSLLADD473 pKa = 5.1DD474 pKa = 5.4ADD476 pKa = 4.06EE477 pKa = 5.36DD478 pKa = 4.45DD479 pKa = 5.64VYY481 pKa = 11.5DD482 pKa = 4.1SPLIVYY488 pKa = 7.71TANRR492 pKa = 11.84PFSFHH497 pKa = 7.16SNTSTFQLSYY507 pKa = 10.9EE508 pKa = 4.25DD509 pKa = 4.27PEE511 pKa = 4.25KK512 pKa = 11.14LSMIRR517 pKa = 11.84NGFEE521 pKa = 3.61TASRR525 pKa = 11.84LNMTLDD531 pKa = 4.71DD532 pKa = 3.67EE533 pKa = 4.79WKK535 pKa = 8.63TCVACAIIRR544 pKa = 11.84RR545 pKa = 11.84EE546 pKa = 3.94QEE548 pKa = 3.65RR549 pKa = 11.84QGIEE553 pKa = 3.89QSDD556 pKa = 3.61QCKK559 pKa = 9.64QCFEE563 pKa = 4.45EE564 pKa = 4.6YY565 pKa = 10.17CWDD568 pKa = 3.58GTIDD572 pKa = 4.1DD573 pKa = 4.75SAPGVNFTTEE583 pKa = 3.73GTTDD587 pKa = 3.49DD588 pKa = 4.9AEE590 pKa = 4.37NTGNVSTSFGYY601 pKa = 10.5SLIRR605 pKa = 11.84SNKK608 pKa = 9.68NYY610 pKa = 10.93ALLQALGYY618 pKa = 10.12GLLAVVVCTYY628 pKa = 10.99SALL631 pKa = 3.72

Molecular weight: 69.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1A0HEQ5|A0A1A0HEQ5_9ASCO Uncharacterized protein OS=Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993 OX=869754 GN=METBIDRAFT_82403 PE=4 SV=1
MM1 pKa = 7.68KK2 pKa = 9.82PRR4 pKa = 11.84PVKK7 pKa = 10.6KK8 pKa = 10.16KK9 pKa = 10.04GRR11 pKa = 11.84SKK13 pKa = 10.72KK14 pKa = 9.5KK15 pKa = 9.2QGRR18 pKa = 11.84FNEE21 pKa = 4.1TRR23 pKa = 11.84PVQKK27 pKa = 10.02KK28 pKa = 9.87GRR30 pKa = 11.84FIKK33 pKa = 9.58TRR35 pKa = 11.84PLRR38 pKa = 11.84YY39 pKa = 9.92NKK41 pKa = 9.6VGQKK45 pKa = 10.05KK46 pKa = 7.85RR47 pKa = 11.84TAPIKK52 pKa = 10.24KK53 pKa = 9.1GRR55 pKa = 11.84SNITGPLQQNKK66 pKa = 9.29AASIKK71 pKa = 10.12QGRR74 pKa = 11.84TKK76 pKa = 10.67KK77 pKa = 9.09RR78 pKa = 11.84TAPTKK83 pKa = 9.87QGRR86 pKa = 11.84FNKK89 pKa = 9.5TGRR92 pKa = 11.84SKK94 pKa = 11.13KK95 pKa = 10.1KK96 pKa = 10.67GPLQKK101 pKa = 10.62KK102 pKa = 7.71KK103 pKa = 8.69TAPTKK108 pKa = 10.26AAPIKK113 pKa = 10.01QSRR116 pKa = 11.84FHH118 pKa = 6.4KK119 pKa = 9.49TRR121 pKa = 11.84AVLIKK126 pKa = 10.5PGLLL130 pKa = 3.47

Molecular weight: 14.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5826

1

5827

2545319

49

4188

436.8

48.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.797 ± 0.045

1.318 ± 0.012

5.739 ± 0.024

6.222 ± 0.03

4.482 ± 0.021

5.717 ± 0.033

2.454 ± 0.013

5.312 ± 0.029

6.089 ± 0.03

10.43 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.101 ± 0.011

4.405 ± 0.022

4.881 ± 0.028

3.844 ± 0.021

5.113 ± 0.024

8.185 ± 0.035

5.29 ± 0.018

6.413 ± 0.024

1.094 ± 0.009

3.113 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski