Paracoccus chinensis
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3544 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G9DV27|A0A1G9DV27_9RHOB Carbohydrate ABC transporter substrate-binding protein CUT1 family (TC 3.A.1.1.-) OS=Paracoccus chinensis OX=525640 GN=SAMN04487971_102231 PE=3 SV=1
MM1 pKa = 7.08 YY2 pKa = 8.1 QTQPTEE8 pKa = 4.17 GEE10 pKa = 4.08 PLIAPSTTDD19 pKa = 2.82 HH20 pKa = 7.15 PLYY23 pKa = 10.78 DD24 pKa = 4.17 SVVEE28 pKa = 4.21 ACRR31 pKa = 11.84 TVYY34 pKa = 10.74 DD35 pKa = 3.74 PEE37 pKa = 5.01 IPVNIFDD44 pKa = 5.17 LGLIYY49 pKa = 10.28 TIAIDD54 pKa = 3.39 DD55 pKa = 4.05 TNAVRR60 pKa = 11.84 ILMTLTAPGCPVAGEE75 pKa = 4.15 MPGWVADD82 pKa = 4.01 AVNAIPGVRR91 pKa = 11.84 QVDD94 pKa = 3.34 VDD96 pKa = 4.09 MVFQPPWGMEE106 pKa = 3.86 MMSDD110 pKa = 3.74 EE111 pKa = 4.65 ARR113 pKa = 11.84 LEE115 pKa = 4.03 LGFII119 pKa = 4.34
Molecular weight: 13.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.738
IPC2_protein 3.732
IPC_protein 3.681
Toseland 3.478
ProMoST 3.859
Dawson 3.668
Bjellqvist 3.834
Wikipedia 3.617
Rodwell 3.516
Grimsley 3.389
Solomon 3.643
Lehninger 3.605
Nozaki 3.808
DTASelect 3.999
Thurlkill 3.541
EMBOSS 3.63
Sillero 3.795
Patrickios 0.769
IPC_peptide 3.643
IPC2_peptide 3.77
IPC2.peptide.svr19 3.732
Protein with the highest isoelectric point:
>tr|A0A1G9DFT4|A0A1G9DFT4_9RHOB SnoaL-like domain-containing protein OS=Paracoccus chinensis OX=525640 GN=SAMN04487971_10247 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.36 GGRR28 pKa = 11.84 KK29 pKa = 8.97 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.71 GRR39 pKa = 11.84 KK40 pKa = 8.73 VLSAA44 pKa = 4.05
Molecular weight: 4.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3544
0
3544
1077329
40
2852
304.0
32.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.589 ± 0.077
0.801 ± 0.014
5.785 ± 0.033
5.781 ± 0.038
3.293 ± 0.025
9.064 ± 0.044
2.026 ± 0.02
4.658 ± 0.029
2.325 ± 0.03
10.329 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.663 ± 0.021
2.217 ± 0.023
5.752 ± 0.038
3.181 ± 0.025
7.902 ± 0.043
4.75 ± 0.027
5.233 ± 0.03
7.292 ± 0.033
1.448 ± 0.017
1.911 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here