Banana mild mosaic virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q993S7|Q993S7_9VIRU Helicase OS=Banana mild mosaic virus OX=148879 PE=3 SV=1
MM1 pKa = 7.43NSSLEE6 pKa = 3.93NLKK9 pKa = 10.2QLIEE13 pKa = 4.26NFGFTATGNPVTDD26 pKa = 4.43KK27 pKa = 11.06IVIHH31 pKa = 6.38GVAGCGKK38 pKa = 8.61STLTKK43 pKa = 10.61EE44 pKa = 4.05LLKK47 pKa = 11.07DD48 pKa = 3.47NNFNIVNALSKK59 pKa = 11.03EE60 pKa = 4.1DD61 pKa = 4.05FDD63 pKa = 5.05ITGQYY68 pKa = 8.88IKK70 pKa = 10.97KK71 pKa = 9.82EE72 pKa = 4.07LTTSDD77 pKa = 3.15SKK79 pKa = 11.61INVLDD84 pKa = 4.28EE85 pKa = 4.17YY86 pKa = 11.44LSVDD90 pKa = 3.46CHH92 pKa = 7.85KK93 pKa = 11.02GFQVLLADD101 pKa = 4.36PFQYY105 pKa = 10.18KK106 pKa = 10.07GKK108 pKa = 9.37PYY110 pKa = 9.93LANYY114 pKa = 8.72IKK116 pKa = 10.62KK117 pKa = 10.0KK118 pKa = 6.85SHH120 pKa = 6.02RR121 pKa = 11.84FKK123 pKa = 11.21KK124 pKa = 10.13EE125 pKa = 3.38LVPILLEE132 pKa = 4.18LGVEE136 pKa = 4.26VEE138 pKa = 4.3VEE140 pKa = 4.07EE141 pKa = 4.54EE142 pKa = 3.97GLNIIRR148 pKa = 11.84GSAYY152 pKa = 9.93EE153 pKa = 4.27IEE155 pKa = 4.55PKK157 pKa = 10.76GKK159 pKa = 9.5IISVEE164 pKa = 4.1EE165 pKa = 3.93EE166 pKa = 3.93VVKK169 pKa = 10.82YY170 pKa = 10.76VSEE173 pKa = 4.52HH174 pKa = 5.7GLEE177 pKa = 3.58IHH179 pKa = 6.45YY180 pKa = 8.17PSCIQGQEE188 pKa = 3.68FDD190 pKa = 3.88VVTFYY195 pKa = 11.13HH196 pKa = 6.92KK197 pKa = 10.68EE198 pKa = 3.82DD199 pKa = 3.51LRR201 pKa = 11.84ALDD204 pKa = 3.74RR205 pKa = 11.84SDD207 pKa = 4.3VYY209 pKa = 11.36VALTRR214 pKa = 11.84VRR216 pKa = 11.84EE217 pKa = 4.02EE218 pKa = 4.32LRR220 pKa = 11.84LLQLL224 pKa = 3.97

Molecular weight: 25.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q993S6|Q993S6_9VIRU Triple gene block protein 2 OS=Banana mild mosaic virus OX=148879 PE=4 SV=1
MM1 pKa = 7.45ALSRR5 pKa = 11.84PPDD8 pKa = 3.5YY9 pKa = 11.15TKK11 pKa = 11.13VLLVSSLAVGTAIIIHH27 pKa = 5.9FLRR30 pKa = 11.84RR31 pKa = 11.84SEE33 pKa = 4.23LPHH36 pKa = 8.19VGDD39 pKa = 4.38NLHH42 pKa = 6.51HH43 pKa = 6.56LPYY46 pKa = 10.5GGSYY50 pKa = 10.83CDD52 pKa = 3.24GTKK55 pKa = 10.25RR56 pKa = 11.84INYY59 pKa = 8.54GGSHH63 pKa = 6.36NKK65 pKa = 9.75HH66 pKa = 6.0SLITFNPLLLVFILSALIYY85 pKa = 9.9VLSKK89 pKa = 10.13RR90 pKa = 11.84DD91 pKa = 3.5SMSVGVHH98 pKa = 5.05NCGAGCHH105 pKa = 4.17VHH107 pKa = 6.3IRR109 pKa = 11.84RR110 pKa = 11.84NRR112 pKa = 3.16

Molecular weight: 12.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2415

70

1771

483.0

55.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.803 ± 1.062

2.029 ± 0.263

5.135 ± 0.467

7.992 ± 0.991

5.507 ± 0.872

4.803 ± 0.711

2.484 ± 0.728

6.832 ± 0.344

8.323 ± 0.815

9.358 ± 0.864

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.609 ± 0.569

6.087 ± 0.402

3.064 ± 0.274

2.816 ± 0.38

5.3 ± 0.511

7.453 ± 0.485

4.472 ± 0.183

6.832 ± 0.902

0.663 ± 0.258

3.437 ± 0.442
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski