Halomonas olivaria
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7365 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3T1DN19|A0A3T1DN19_9GAMM Uncharacterized protein OS=Halomonas olivaria OX=390919 GN=HORIV_24070 PE=4 SV=1
MM1 pKa = 7.07 QGKK4 pKa = 9.31 HH5 pKa = 4.5 YY6 pKa = 10.57 SSRR9 pKa = 11.84 GDD11 pKa = 3.91 LEE13 pKa = 4.45 PSDD16 pKa = 4.01 GSEE19 pKa = 3.82 VAAQGGVRR27 pKa = 11.84 YY28 pKa = 9.19 AQGPVTLGLGFVDD41 pKa = 6.13 DD42 pKa = 4.01 IVEE45 pKa = 4.35 GGGNDD50 pKa = 3.28 EE51 pKa = 4.21 VRR53 pKa = 11.84 YY54 pKa = 10.03 GLIGSYY60 pKa = 11.09 DD61 pKa = 3.45 FTDD64 pKa = 3.27 QFSARR69 pKa = 11.84 LGYY72 pKa = 7.52 EE73 pKa = 3.86 TRR75 pKa = 11.84 DD76 pKa = 3.4 EE77 pKa = 4.27 NDD79 pKa = 3.15 NYY81 pKa = 10.43 TPSATGEE88 pKa = 4.05 NGFDD92 pKa = 3.55 TVGLGGTFTTIPWAFALDD110 pKa = 4.19 YY111 pKa = 11.57 YY112 pKa = 11.44 NIDD115 pKa = 3.15 RR116 pKa = 11.84 DD117 pKa = 4.16 NRR119 pKa = 11.84 DD120 pKa = 4.33 DD121 pKa = 4.08 SNAWAAGSYY130 pKa = 10.53 YY131 pKa = 10.39 KK132 pKa = 10.72 VSSNFDD138 pKa = 3.36 VFLEE142 pKa = 4.43 LADD145 pKa = 4.16 GDD147 pKa = 4.28 APTVNRR153 pKa = 11.84 DD154 pKa = 3.14 TTLGDD159 pKa = 3.98 LSDD162 pKa = 3.82 DD163 pKa = 4.37 VYY165 pKa = 10.82 WLTGARR171 pKa = 11.84 YY172 pKa = 9.1 HH173 pKa = 6.65 FF174 pKa = 4.65
Molecular weight: 19.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.734
IPC2_protein 3.872
IPC_protein 3.884
Toseland 3.656
ProMoST 4.062
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.846
Rodwell 3.706
Grimsley 3.567
Solomon 3.884
Lehninger 3.846
Nozaki 4.012
DTASelect 4.291
Thurlkill 3.719
EMBOSS 3.859
Sillero 4.012
Patrickios 1.926
IPC_peptide 3.872
IPC2_peptide 3.986
IPC2.peptide.svr19 3.904
Protein with the highest isoelectric point:
>tr|A0A3T1DGK3|A0A3T1DGK3_9GAMM Uncharacterized protein OS=Halomonas olivaria OX=390919 GN=HORIV_02040 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.42 RR12 pKa = 11.84 KK13 pKa = 9.04 RR14 pKa = 11.84 AHH16 pKa = 6.12 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.17 NGRR28 pKa = 11.84 AILARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.95 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.705
Grimsley 13.042
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.427
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7365
0
7365
1383111
39
1475
187.8
20.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.769 ± 0.044
1.098 ± 0.012
5.232 ± 0.025
6.086 ± 0.029
3.53 ± 0.021
7.69 ± 0.032
2.402 ± 0.02
5.114 ± 0.029
3.012 ± 0.025
11.129 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.855 ± 0.018
3.015 ± 0.018
4.929 ± 0.025
4.258 ± 0.025
6.463 ± 0.033
6.091 ± 0.027
5.29 ± 0.023
6.981 ± 0.027
1.564 ± 0.016
2.492 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here