Myotis davidii (David s myotis)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Laurasiatheria; Chiroptera; Microchiroptera; Vespertilionidae;

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15446 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L5LL44|L5LL44_MYODS Cytochrome P450 2J1 OS=Myotis davidii OX=225400 GN=MDA_GLEAN10024042 PE=3 SV=1
MM1 pKa = 6.91PTGVKK6 pKa = 9.35LQKK9 pKa = 10.34PGTEE13 pKa = 3.86PLQCEE18 pKa = 4.33RR19 pKa = 11.84AVHH22 pKa = 5.92GGEE25 pKa = 4.35AGDD28 pKa = 3.59VTNCKK33 pKa = 9.84KK34 pKa = 10.75DD35 pKa = 3.32NCKK38 pKa = 10.61YY39 pKa = 10.78GPNCGQEE46 pKa = 4.39DD47 pKa = 4.29ADD49 pKa = 4.15GDD51 pKa = 4.61GIGDD55 pKa = 4.07ACDD58 pKa = 3.53EE59 pKa = 4.74DD60 pKa = 5.69ADD62 pKa = 4.44GDD64 pKa = 4.65GILNEE69 pKa = 4.54DD70 pKa = 3.84NCVLTHH76 pKa = 7.18NMDD79 pKa = 4.32QSDD82 pKa = 4.01SDD84 pKa = 3.37QGIFGDD90 pKa = 3.92ACDD93 pKa = 3.58NCRR96 pKa = 11.84DD97 pKa = 4.18VLNNDD102 pKa = 3.46QKK104 pKa = 11.28DD105 pKa = 3.38TDD107 pKa = 3.94GDD109 pKa = 4.23GKK111 pKa = 11.17GGACDD116 pKa = 5.29DD117 pKa = 5.11DD118 pKa = 3.95MDD120 pKa = 6.2GDD122 pKa = 4.52GIKK125 pKa = 9.84TILDD129 pKa = 3.56SCQEE133 pKa = 4.22VPNHH137 pKa = 5.97DD138 pKa = 3.47QRR140 pKa = 11.84TGQGWRR146 pKa = 11.84WCGGCLL152 pKa = 4.53

Molecular weight: 16.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L5M3V6|L5M3V6_MYODS Anaphase-promoting complex subunit 16 OS=Myotis davidii OX=225400 GN=MDA_GLEAN10020866 PE=3 SV=1
MM1 pKa = 7.68SGPQNLDD8 pKa = 3.17LAPILRR14 pKa = 11.84AAGLLGANSASFSQASGNMGTSPSSMARR42 pKa = 11.84VPGPIGPNLGPGSRR56 pKa = 11.84GIGLPGPNPSPMSRR70 pKa = 11.84ASGPIGPNSAHH81 pKa = 7.19FSRR84 pKa = 11.84PAGPMGVNTNPFPRR98 pKa = 11.84GTGSGALHH106 pKa = 6.88PAAFSQSSGTLASNPPTFQRR126 pKa = 11.84SAGFQGSNPAVFPRR140 pKa = 11.84ASGPLGPNPANFPRR154 pKa = 11.84APGLQGPSPATFPRR168 pKa = 11.84STGPLGSGQVTFPRR182 pKa = 11.84SASGPLGSSAGPGGINPAPFARR204 pKa = 11.84PTGTLGLNPASFPRR218 pKa = 11.84MNGPIMKK225 pKa = 8.86TVVPFPRR232 pKa = 11.84VGSLPGTNPAAFPRR246 pKa = 11.84PGGPMSAMYY255 pKa = 9.6PNGMLPPP262 pKa = 4.73

Molecular weight: 25.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15446

0

15446

7428624

26

31357

480.9

53.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.04 ± 0.021

2.217 ± 0.016

4.804 ± 0.016

7.01 ± 0.027

3.594 ± 0.015

6.518 ± 0.025

2.647 ± 0.012

4.261 ± 0.018

5.608 ± 0.028

9.981 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.178 ± 0.009

3.496 ± 0.013

6.239 ± 0.028

4.751 ± 0.021

5.696 ± 0.019

8.177 ± 0.028

5.26 ± 0.021

6.129 ± 0.018

1.236 ± 0.008

2.591 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski