Dragonfly-associated circular virus 2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus draga1; Dragonfly associated gemycircularvirus 1

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K0A2R4|K0A2R4_9VIRU Replication-associated protein OS=Dragonfly-associated circular virus 2 OX=1234886 PE=3 SV=1
MM1 pKa = 7.81PSFVCNARR9 pKa = 11.84YY10 pKa = 9.04FLVTYY15 pKa = 7.11PQCGNLCGFSVVEE28 pKa = 4.33RR29 pKa = 11.84FSSLGAEE36 pKa = 4.2CIVGRR41 pKa = 11.84EE42 pKa = 3.95HH43 pKa = 8.11HH44 pKa = 6.88EE45 pKa = 4.43DD46 pKa = 3.48GGLHH50 pKa = 5.8LHH52 pKa = 6.83CFADD56 pKa = 4.75FGRR59 pKa = 11.84KK60 pKa = 8.15FRR62 pKa = 11.84SRR64 pKa = 11.84KK65 pKa = 7.67TDD67 pKa = 2.69IFDD70 pKa = 4.44VDD72 pKa = 3.66GCHH75 pKa = 7.11PNIQPSTGTPEE86 pKa = 3.53KK87 pKa = 10.7GYY89 pKa = 10.88DD90 pKa = 3.47YY91 pKa = 10.69AIKK94 pKa = 10.87DD95 pKa = 3.59GDD97 pKa = 4.01VVAGGLEE104 pKa = 3.96RR105 pKa = 11.84PIGKK109 pKa = 9.14SRR111 pKa = 11.84AGARR115 pKa = 11.84TTFDD119 pKa = 2.52KK120 pKa = 10.51WAEE123 pKa = 3.75ITGAEE128 pKa = 4.61DD129 pKa = 5.92RR130 pKa = 11.84DD131 pKa = 4.54TFWQLCHH138 pKa = 6.67EE139 pKa = 5.52LDD141 pKa = 4.23PKK143 pKa = 10.38ATACSFGQLSKK154 pKa = 10.58YY155 pKa = 10.59ADD157 pKa = 2.97WRR159 pKa = 11.84FAEE162 pKa = 4.65VPAEE166 pKa = 3.97YY167 pKa = 9.83EE168 pKa = 4.28SPGGLEE174 pKa = 4.46FIGGDD179 pKa = 3.16VDD181 pKa = 6.0GRR183 pKa = 11.84DD184 pKa = 3.69DD185 pKa = 3.57WVQSAGIGLGQSFVGGG201 pKa = 3.64

Molecular weight: 22.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K0A172|K0A172_9VIRU Replication-associated protein OS=Dragonfly-associated circular virus 2 OX=1234886 PE=4 SV=1
MM1 pKa = 7.74AYY3 pKa = 10.14ARR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84SGSYY11 pKa = 9.12RR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84QTKK18 pKa = 9.77RR19 pKa = 11.84RR20 pKa = 11.84GTGYY24 pKa = 9.98RR25 pKa = 11.84RR26 pKa = 11.84SSAKK30 pKa = 7.15TRR32 pKa = 11.84RR33 pKa = 11.84YY34 pKa = 10.02RR35 pKa = 11.84RR36 pKa = 11.84TKK38 pKa = 8.45RR39 pKa = 11.84TSRR42 pKa = 11.84PMSKK46 pKa = 9.64RR47 pKa = 11.84RR48 pKa = 11.84MLNVSSRR55 pKa = 11.84KK56 pKa = 9.33KK57 pKa = 10.14RR58 pKa = 11.84NGMLSWSNTASTGASQTVASGPAYY82 pKa = 11.06VNGTGTGFFLWNATANDD99 pKa = 4.14LNSGNGAYY107 pKa = 8.32NTIAQEE113 pKa = 4.23SQRR116 pKa = 11.84TSTTCYY122 pKa = 8.95MRR124 pKa = 11.84GLSEE128 pKa = 5.06HH129 pKa = 6.59IRR131 pKa = 11.84IQTSSGLPWFWRR143 pKa = 11.84RR144 pKa = 11.84ICFTFRR150 pKa = 11.84GSFPAASDD158 pKa = 3.95SPTQTPRR165 pKa = 11.84NFQDD169 pKa = 3.09TSNGIVRR176 pKa = 11.84LWFNTDD182 pKa = 2.87VNNTPNLRR190 pKa = 11.84NQVDD194 pKa = 3.9GVIFKK199 pKa = 10.19GAKK202 pKa = 9.04GVDD205 pKa = 3.05WNDD208 pKa = 4.11FIVAPVDD215 pKa = 3.41TRR217 pKa = 11.84RR218 pKa = 11.84VDD220 pKa = 3.72LKK222 pKa = 10.86YY223 pKa = 11.03DD224 pKa = 3.0RR225 pKa = 11.84TMCIRR230 pKa = 11.84SGNAAGTVKK239 pKa = 10.09EE240 pKa = 4.1AKK242 pKa = 9.2LWHH245 pKa = 6.12PMNRR249 pKa = 11.84NLVYY253 pKa = 10.84DD254 pKa = 4.54DD255 pKa = 5.66DD256 pKa = 4.69EE257 pKa = 6.97SGDD260 pKa = 3.88QEE262 pKa = 4.15ATSYY266 pKa = 11.48LSVEE270 pKa = 4.7SKK272 pKa = 11.19AGMGDD277 pKa = 4.12YY278 pKa = 10.84YY279 pKa = 11.67VLDD282 pKa = 4.3LLQAGSGGSNSDD294 pKa = 4.45QILLQANSTLYY305 pKa = 9.39WHH307 pKa = 7.05EE308 pKa = 4.08KK309 pKa = 9.32

Molecular weight: 34.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

920

91

319

230.0

25.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.609 ± 0.138

2.174 ± 0.614

7.065 ± 0.565

5.0 ± 1.018

5.326 ± 0.74

9.891 ± 1.0

2.174 ± 0.451

4.457 ± 0.67

4.565 ± 0.271

5.87 ± 0.162

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.522 ± 0.437

4.022 ± 1.175

4.348 ± 0.53

2.717 ± 0.48

7.717 ± 1.161

8.043 ± 0.853

5.543 ± 1.133

5.87 ± 0.393

2.717 ± 0.241

3.37 ± 0.293
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski