Aeromonas phage 59.1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 87 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A219YA95|A0A219YA95_9CAUD Uncharacterized protein OS=Aeromonas phage 59.1 OX=1932903 PE=4 SV=1
MM1 pKa = 7.5EE2 pKa = 5.51IEE4 pKa = 4.65YY5 pKa = 8.86LTNDD9 pKa = 4.03NEE11 pKa = 3.79RR12 pKa = 11.84HH13 pKa = 5.71AYY15 pKa = 7.55VTGHH19 pKa = 6.2VDD21 pKa = 2.99TGQFMAAVVNDD32 pKa = 4.8GYY34 pKa = 10.94EE35 pKa = 3.64ISHH38 pKa = 6.63FEE40 pKa = 3.65GHH42 pKa = 5.35GWMVEE47 pKa = 4.05LPVPEE52 pKa = 4.82DD53 pKa = 3.05LAAEE57 pKa = 4.29FEE59 pKa = 4.49RR60 pKa = 11.84WFEE63 pKa = 3.9FCNEE67 pKa = 3.9GEE69 pKa = 4.23EE70 pKa = 4.79GSFAVTWASVYY81 pKa = 10.91

Molecular weight: 9.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A219YA53|A0A219YA53_9CAUD Uncharacterized protein OS=Aeromonas phage 59.1 OX=1932903 PE=4 SV=1
MM1 pKa = 7.39PVIVPVIATSIATGQVVRR19 pKa = 11.84FASVTAAARR28 pKa = 11.84DD29 pKa = 3.6GGFDD33 pKa = 3.33RR34 pKa = 11.84TCVSNCVNGYY44 pKa = 9.49RR45 pKa = 11.84KK46 pKa = 9.41SHH48 pKa = 6.27NGFTFKK54 pKa = 10.69ADD56 pKa = 3.39CKK58 pKa = 10.53LRR60 pKa = 11.84PTGSDD65 pKa = 3.18TLIHH69 pKa = 6.86KK70 pKa = 9.95IAALRR75 pKa = 11.84NTGHH79 pKa = 6.21NTTEE83 pKa = 3.55IAAIMGLSASTVRR96 pKa = 11.84NRR98 pKa = 11.84VPMARR103 pKa = 11.84NIGLLTKK110 pKa = 10.62

Molecular weight: 11.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

87

0

87

13917

38

828

160.0

17.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.16 ± 0.475

1.272 ± 0.129

5.461 ± 0.207

6.165 ± 0.358

3.363 ± 0.175

7.99 ± 0.355

1.962 ± 0.133

5.274 ± 0.202

5.77 ± 0.288

8.371 ± 0.275

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.557 ± 0.168

3.521 ± 0.246

4.283 ± 0.26

3.435 ± 0.149

6.036 ± 0.282

6.158 ± 0.232

5.878 ± 0.271

7.293 ± 0.282

1.602 ± 0.114

2.436 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski