Hubei astro-like virus

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KNZ3|A0A1L3KNZ3_9VIRU Capsid protein OS=Hubei astro-like virus OX=1922837 PE=4 SV=1
MM1 pKa = 7.49ANVGGTEE8 pKa = 3.76ARR10 pKa = 11.84RR11 pKa = 11.84EE12 pKa = 3.93PGGNGWVNIYY22 pKa = 10.65KK23 pKa = 10.43KK24 pKa = 10.53LKK26 pKa = 10.06KK27 pKa = 10.02ALKK30 pKa = 10.37DD31 pKa = 3.48PSSNQAVNLLSDD43 pKa = 4.35LVSGIEE49 pKa = 3.92QSLTAITRR57 pKa = 11.84EE58 pKa = 4.16TLMSTALSVLRR69 pKa = 11.84GKK71 pKa = 9.88LLNMVLTWIFFTIWVAFPYY90 pKa = 10.07VPRR93 pKa = 11.84VEE95 pKa = 4.79IDD97 pKa = 3.19LLLNYY102 pKa = 9.83DD103 pKa = 3.89LHH105 pKa = 7.41CFQLVEE111 pKa = 4.04NLAVVEE117 pKa = 4.08LVFRR121 pKa = 11.84AWFWYY126 pKa = 9.69KK127 pKa = 10.55LAFTFYY133 pKa = 10.1SLVYY137 pKa = 9.93FVTDD141 pKa = 3.51CLRR144 pKa = 11.84AYY146 pKa = 8.69WRR148 pKa = 11.84YY149 pKa = 8.9QSLRR153 pKa = 11.84EE154 pKa = 4.06LVWQVRR160 pKa = 11.84WTLMSCSNDD169 pKa = 2.9EE170 pKa = 5.6RR171 pKa = 11.84LDD173 pKa = 3.48DD174 pKa = 3.9WEE176 pKa = 5.07GMFDD180 pKa = 4.71LICSIFSPLCACAGTSAVNQWLTSYY205 pKa = 10.24TFGKK209 pKa = 10.43KK210 pKa = 9.91SFFATRR216 pKa = 11.84GASNFVQGYY225 pKa = 8.29IGRR228 pKa = 11.84NLGEE232 pKa = 4.17TVYY235 pKa = 10.77NLCVPRR241 pKa = 11.84AFQKK245 pKa = 10.54SDD247 pKa = 2.84CHH249 pKa = 6.21TWRR252 pKa = 11.84CWVRR256 pKa = 11.84PYY258 pKa = 10.64PGARR262 pKa = 11.84LLQHH266 pKa = 6.55CNHH269 pKa = 6.35RR270 pKa = 11.84MVLAEE275 pKa = 3.98QGVDD279 pKa = 2.98AADD282 pKa = 3.9FMSPGMFLLVPLMFVGWCVRR302 pKa = 11.84RR303 pKa = 11.84VFKK306 pKa = 10.16GTEE309 pKa = 3.83SRR311 pKa = 11.84QGLPSLTRR319 pKa = 11.84YY320 pKa = 10.22DD321 pKa = 3.83CLTPLRR327 pKa = 11.84GPTPEE332 pKa = 4.7SIFPGSPRR340 pKa = 11.84IGAVTKK346 pKa = 10.68AADD349 pKa = 3.03ATAAVLHH356 pKa = 6.68RR357 pKa = 11.84GTRR360 pKa = 11.84VATCFFVGNTIVTCKK375 pKa = 9.98HH376 pKa = 5.99AYY378 pKa = 9.75EE379 pKa = 4.51PDD381 pKa = 3.2EE382 pKa = 4.55EE383 pKa = 4.73GFHH386 pKa = 6.65FSFRR390 pKa = 11.84GVDD393 pKa = 3.32WTLKK397 pKa = 10.49SPVAKK402 pKa = 10.26FFPASVGCEE411 pKa = 3.94EE412 pKa = 4.23IVVVSMPKK420 pKa = 10.49DD421 pKa = 3.53LVSGLLNFGAMRR433 pKa = 11.84FKK435 pKa = 10.23TRR437 pKa = 11.84KK438 pKa = 9.73LVDD441 pKa = 3.24KK442 pKa = 9.79TAGGSIGYY450 pKa = 8.74SASNMYY456 pKa = 10.09HH457 pKa = 7.04LSFATGTDD465 pKa = 3.02IDD467 pKa = 4.37PDD469 pKa = 3.76GTHH472 pKa = 6.94NISSSPGSSGSPVFDD487 pKa = 4.29CNGIAVGVHH496 pKa = 5.11NAGGAVSNFFLPFSDD511 pKa = 4.55DD512 pKa = 3.65CIGYY516 pKa = 8.02MRR518 pKa = 11.84TTAPVDD524 pKa = 3.27GSVVVTAIHH533 pKa = 6.31GGWDD537 pKa = 3.2ATKK540 pKa = 8.72PTDD543 pKa = 3.03KK544 pKa = 10.17GTPVHH549 pKa = 6.61VLDD552 pKa = 5.22NVNTNLGSSSVGKK565 pKa = 9.86NDD567 pKa = 3.0AYY569 pKa = 9.69TRR571 pKa = 11.84CEE573 pKa = 4.0QFVSWATNSTNQQAWDD589 pKa = 3.67DD590 pKa = 3.8EE591 pKa = 4.8RR592 pKa = 11.84NPRR595 pKa = 11.84RR596 pKa = 11.84PKK598 pKa = 10.44AVPGTCASRR607 pKa = 11.84NAPVVSSVVTTVANNHH623 pKa = 6.05SGCNTVDD630 pKa = 3.85ADD632 pKa = 4.1HH633 pKa = 7.19SDD635 pKa = 3.15IFDD638 pKa = 3.5ARR640 pKa = 11.84VAQSIVSFYY649 pKa = 11.14KK650 pKa = 10.34EE651 pKa = 4.0YY652 pKa = 10.53TGPTLAAKK660 pKa = 10.24LDD662 pKa = 4.8AILHH666 pKa = 5.59SVLTDD671 pKa = 3.38HH672 pKa = 7.28FDD674 pKa = 3.46SDD676 pKa = 4.06MVDD679 pKa = 3.53KK680 pKa = 11.25LRR682 pKa = 11.84VAYY685 pKa = 9.39PGLNDD690 pKa = 3.47DD691 pKa = 4.91QIWARR696 pKa = 11.84ISKK699 pKa = 10.15RR700 pKa = 11.84CADD703 pKa = 3.75PGAIVVGSHH712 pKa = 5.27SQATAVVAALVEE724 pKa = 4.48GARR727 pKa = 11.84TIYY730 pKa = 9.96PDD732 pKa = 3.27EE733 pKa = 4.39WSDD736 pKa = 3.67EE737 pKa = 4.15YY738 pKa = 11.35LGSWLHH744 pKa = 5.91QRR746 pKa = 11.84YY747 pKa = 7.92PSCYY751 pKa = 7.75RR752 pKa = 11.84PANIPHH758 pKa = 6.11SVSDD762 pKa = 3.74QDD764 pKa = 4.4NQPACPNN771 pKa = 3.5

Molecular weight: 85.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KNT2|A0A1L3KNT2_9VIRU RNA-dependent RNA polymerase OS=Hubei astro-like virus OX=1922837 PE=4 SV=1
MM1 pKa = 7.8RR2 pKa = 11.84RR3 pKa = 11.84LLCYY7 pKa = 9.23TVALVLPLAFSLAILSSRR25 pKa = 11.84VSTRR29 pKa = 11.84MNRR32 pKa = 11.84TKK34 pKa = 10.38RR35 pKa = 11.84VSISLFAEE43 pKa = 4.71WIGLLSHH50 pKa = 7.02LWLSSFPLLLGVRR63 pKa = 11.84KK64 pKa = 9.92SLWFLCQKK72 pKa = 7.61TWCLVCSILVPCVLRR87 pKa = 11.84PGSWLIKK94 pKa = 9.87RR95 pKa = 11.84LVGQSVILLLTCTILVLLQEE115 pKa = 5.16PILTQMALITSLRR128 pKa = 11.84LPEE131 pKa = 4.12AVEE134 pKa = 4.1VLSSIVTGLPSVYY147 pKa = 10.32IMLAVRR153 pKa = 11.84SATSSYY159 pKa = 10.95PLVTTVLVTCVLLHH173 pKa = 6.21QLTDD177 pKa = 3.07QWLLPLFMVVGMPLSQLTKK196 pKa = 10.1VLQYY200 pKa = 9.39TYY202 pKa = 10.26WIMLIPIWGLHH213 pKa = 4.45LWGRR217 pKa = 11.84MMPTPDD223 pKa = 4.25ASSLYY228 pKa = 9.22PGPLIALTNKK238 pKa = 10.28LGTTSVIQEE247 pKa = 4.03GQRR250 pKa = 11.84LYY252 pKa = 11.11LEE254 pKa = 4.43PVQVEE259 pKa = 4.23MLLSYY264 pKa = 10.36PVLLLLWLITTLAVTLSMLTIRR286 pKa = 11.84TSLMRR291 pKa = 11.84VLHH294 pKa = 6.32RR295 pKa = 11.84ALSPFTKK302 pKa = 10.55NILVQPWLLSWTLFCTACSQITLIVTWSTSSAWLILGSMMTKK344 pKa = 10.55FGQGYY349 pKa = 9.31QSAALTLVRR358 pKa = 11.84LWWDD362 pKa = 3.57LTRR365 pKa = 11.84KK366 pKa = 8.62PRR368 pKa = 11.84LWLQRR373 pKa = 11.84WWKK376 pKa = 10.56GPGPFILMNGQTSTLGLGYY395 pKa = 9.31TKK397 pKa = 9.63GTQVAIVPRR406 pKa = 11.84TSHH409 pKa = 5.7TALAIKK415 pKa = 7.16TTNQPAQTRR424 pKa = 11.84PSRR427 pKa = 11.84HH428 pKa = 5.14GFRR431 pKa = 11.84PVKK434 pKa = 9.83FVSGGSTT441 pKa = 2.89

Molecular weight: 49.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2502

441

771

625.5

69.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.114 ± 0.778

1.918 ± 0.499

4.756 ± 0.823

3.597 ± 0.557

3.917 ± 0.397

6.875 ± 0.562

2.278 ± 0.362

4.836 ± 0.463

4.117 ± 0.711

9.592 ± 1.963

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.478 ± 0.272

3.597 ± 0.549

5.516 ± 0.381

3.637 ± 0.368

5.915 ± 0.567

7.874 ± 0.872

7.394 ± 0.96

7.634 ± 0.717

2.598 ± 0.483

3.357 ± 0.531
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski