Gracilimonas mengyeensis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Balneolaeota; Balneolia; Balneolales; Balneolaceae; Gracilimonas

Average proteome isoelectric point is 5.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3810 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A521C3D4|A0A521C3D4_9BACT Uncharacterized protein OS=Gracilimonas mengyeensis OX=1302730 GN=SAMN06265219_104120 PE=4 SV=1
MM1 pKa = 7.81GIFPYY6 pKa = 10.55AQAQEE11 pKa = 4.36VVTTFDD17 pKa = 4.39LNGADD22 pKa = 3.11GTLYY26 pKa = 10.77NDD28 pKa = 3.91EE29 pKa = 4.29NRR31 pKa = 11.84GADD34 pKa = 3.62FPGGDD39 pKa = 3.42RR40 pKa = 11.84SSLEE44 pKa = 3.14IRR46 pKa = 11.84YY47 pKa = 8.55WDD49 pKa = 3.45EE50 pKa = 3.6VRR52 pKa = 11.84FRR54 pKa = 11.84ASMVRR59 pKa = 11.84FDD61 pKa = 3.93VNAVNSSPDD70 pKa = 3.41GAVLGLYY77 pKa = 6.87PTSAGSMGADD87 pKa = 2.53SLTFTIYY94 pKa = 11.2GLTNEE99 pKa = 5.67SEE101 pKa = 4.7DD102 pKa = 3.49NWDD105 pKa = 3.74EE106 pKa = 4.37GSFSYY111 pKa = 11.3NEE113 pKa = 3.94APGFEE118 pKa = 4.04AAANGTYY125 pKa = 10.29AINEE129 pKa = 4.35DD130 pKa = 4.4LDD132 pKa = 3.9SLTTITVYY140 pKa = 11.35NNLTDD145 pKa = 3.44TYY147 pKa = 10.66LYY149 pKa = 10.47SEE151 pKa = 5.19PSAEE155 pKa = 4.21LDD157 pKa = 3.42AFINSDD163 pKa = 3.67TNGLVTFAIVKK174 pKa = 8.7TYY176 pKa = 8.63STEE179 pKa = 3.77SGSYY183 pKa = 9.91VISPKK188 pKa = 10.36EE189 pKa = 3.86AGEE192 pKa = 4.28SVAPKK197 pKa = 10.26LVYY200 pKa = 10.12QSEE203 pKa = 4.32NGEE206 pKa = 3.88PVLPNTEE213 pKa = 3.85PSITLDD219 pKa = 3.53SPTDD223 pKa = 3.59GASYY227 pKa = 10.59EE228 pKa = 4.52SNNTLTASASASDD241 pKa = 3.82SDD243 pKa = 4.64GEE245 pKa = 4.11IASVSFYY252 pKa = 11.17LDD254 pKa = 3.19DD255 pKa = 4.0TEE257 pKa = 5.04IGSVNASPFEE267 pKa = 4.0YY268 pKa = 10.47EE269 pKa = 4.31IDD271 pKa = 3.41LSMYY275 pKa = 11.01ALGSHH280 pKa = 6.47EE281 pKa = 4.84FYY283 pKa = 10.85AIAEE287 pKa = 4.53DD288 pKa = 3.58NDD290 pKa = 4.01GAVASSDD297 pKa = 2.95TVMFEE302 pKa = 3.34ITEE305 pKa = 4.74AIGGSDD311 pKa = 3.57AEE313 pKa = 4.32MVTTFEE319 pKa = 5.57LNGADD324 pKa = 3.21AAIYY328 pKa = 10.86NDD330 pKa = 4.0DD331 pKa = 4.2NRR333 pKa = 11.84GADD336 pKa = 3.46YY337 pKa = 10.84TGGNRR342 pKa = 11.84SDD344 pKa = 3.97LEE346 pKa = 3.55IRR348 pKa = 11.84YY349 pKa = 8.63WEE351 pKa = 4.1EE352 pKa = 3.3VRR354 pKa = 11.84FRR356 pKa = 11.84ASILRR361 pKa = 11.84FDD363 pKa = 4.01VSNVSSTPNGAVIGFNPTYY382 pKa = 9.47AGKK385 pKa = 9.24MGAEE389 pKa = 4.06SLTYY393 pKa = 9.73TIYY396 pKa = 11.33GMTDD400 pKa = 3.17EE401 pKa = 5.82SQDD404 pKa = 3.28NWDD407 pKa = 4.08EE408 pKa = 4.17GSVNYY413 pKa = 10.46SSAPGFEE420 pKa = 4.36SAANGTYY427 pKa = 11.19ALTEE431 pKa = 4.14NMDD434 pKa = 3.96SLTTITVYY442 pKa = 11.15ADD444 pKa = 3.02STGAFLYY451 pKa = 10.48SDD453 pKa = 4.62PSTEE457 pKa = 4.2LDD459 pKa = 3.22AFINNDD465 pKa = 3.25SNGLVTFAIIKK476 pKa = 8.02TISTNSDD483 pKa = 3.64YY484 pKa = 11.66YY485 pKa = 11.89AMNSKK490 pKa = 10.02EE491 pKa = 4.18AGVSVAPRR499 pKa = 11.84LVYY502 pKa = 10.33QSEE505 pKa = 4.15NGEE508 pKa = 3.83PVLPNFEE515 pKa = 4.23PSITLNSPEE524 pKa = 4.31EE525 pKa = 4.11GMVYY529 pKa = 10.43QSDD532 pKa = 3.83STLTARR538 pKa = 11.84ATAIDD543 pKa = 3.86SDD545 pKa = 4.59GEE547 pKa = 4.04IASVSFYY554 pKa = 10.55MDD556 pKa = 3.52GTAVANVTSSPYY568 pKa = 10.15EE569 pKa = 4.01SEE571 pKa = 4.32IDD573 pKa = 3.36LSMYY577 pKa = 11.03ASGSYY582 pKa = 9.67EE583 pKa = 3.93FYY585 pKa = 10.83AIAEE589 pKa = 4.59DD590 pKa = 4.87DD591 pKa = 4.46DD592 pKa = 4.59GATASSDD599 pKa = 3.25TVTIDD604 pKa = 2.94IVGGSGAGSSEE615 pKa = 3.94MVTTFDD621 pKa = 4.66SNGADD626 pKa = 2.97VTLFNDD632 pKa = 3.55EE633 pKa = 3.94NRR635 pKa = 11.84AGSFTGGDD643 pKa = 3.23RR644 pKa = 11.84TDD646 pKa = 3.56LEE648 pKa = 4.06VRR650 pKa = 11.84YY651 pKa = 9.19WEE653 pKa = 4.03AVRR656 pKa = 11.84FRR658 pKa = 11.84ASMLRR663 pKa = 11.84FDD665 pKa = 4.29VSSVSAAPDD674 pKa = 3.46GAVIGINPTYY684 pKa = 10.4AEE686 pKa = 4.07NMSDD690 pKa = 3.29EE691 pKa = 4.5TLSFTIFGLTNEE703 pKa = 5.5AEE705 pKa = 4.42DD706 pKa = 3.78EE707 pKa = 3.99WDD709 pKa = 3.72EE710 pKa = 4.8GSISYY715 pKa = 10.81NSAPGFEE722 pKa = 4.45SAINGSYY729 pKa = 10.59AINDD733 pKa = 4.15DD734 pKa = 4.36LDD736 pKa = 3.82SLTTITVYY744 pKa = 11.14ADD746 pKa = 3.13STGTFLYY753 pKa = 10.57SEE755 pKa = 4.96PSDD758 pKa = 3.75KK759 pKa = 11.07LDD761 pKa = 3.72AFIRR765 pKa = 11.84NDD767 pKa = 3.23NNGLVTFAIIRR778 pKa = 11.84TYY780 pKa = 11.43SNNSDD785 pKa = 3.18IYY787 pKa = 11.42AMSSKK792 pKa = 10.42EE793 pKa = 3.87AGEE796 pKa = 4.01LVAPRR801 pKa = 11.84LVYY804 pKa = 10.38KK805 pKa = 10.5SEE807 pKa = 4.04NGVPVIPNLEE817 pKa = 4.1PNITLSRR824 pKa = 11.84PADD827 pKa = 3.57GVSYY831 pKa = 10.26EE832 pKa = 4.36SNSMLTALASASDD845 pKa = 4.17PDD847 pKa = 4.19GEE849 pKa = 4.16IANVSFYY856 pKa = 10.52MDD858 pKa = 3.44GNEE861 pKa = 4.21IGSATSSPYY870 pKa = 10.23EE871 pKa = 3.91YY872 pKa = 10.44EE873 pKa = 3.89IDD875 pKa = 3.54LSIYY879 pKa = 10.27EE880 pKa = 4.15LGSYY884 pKa = 9.91EE885 pKa = 4.06FFAIAEE891 pKa = 4.52DD892 pKa = 4.29DD893 pKa = 4.32DD894 pKa = 4.58GALASSDD901 pKa = 3.37TVTFEE906 pKa = 4.36IIEE909 pKa = 4.41GNGGGSEE916 pKa = 4.03LPARR920 pKa = 11.84VMEE923 pKa = 5.34DD924 pKa = 2.7IDD926 pKa = 4.26RR927 pKa = 11.84GLIAIDD933 pKa = 4.03RR934 pKa = 11.84GDD936 pKa = 4.98DD937 pKa = 3.4IYY939 pKa = 11.52LSWRR943 pKa = 11.84LLGKK947 pKa = 8.89EE948 pKa = 3.98APDD951 pKa = 3.59LGFNIYY957 pKa = 10.22RR958 pKa = 11.84DD959 pKa = 3.69GTLVNSEE966 pKa = 5.0PITDD970 pKa = 3.44RR971 pKa = 11.84TNYY974 pKa = 9.86VDD976 pKa = 4.6ADD978 pKa = 3.76GSADD982 pKa = 3.38ANYY985 pKa = 10.9SVIPVEE991 pKa = 5.27DD992 pKa = 3.63GTEE995 pKa = 3.97LSEE998 pKa = 4.6SEE1000 pKa = 3.99ATASVWANSFIDD1012 pKa = 3.42IPLNIPADD1020 pKa = 3.93GTHH1023 pKa = 6.4GGSYY1027 pKa = 10.31SPNDD1031 pKa = 3.59MSIGDD1036 pKa = 4.07LDD1038 pKa = 3.95GDD1040 pKa = 3.88NEE1042 pKa = 4.34YY1043 pKa = 11.23EE1044 pKa = 5.87LVLKK1048 pKa = 9.36WYY1050 pKa = 9.32PSNAKK1055 pKa = 10.5DD1056 pKa = 3.52NAQDD1060 pKa = 3.49GLTDD1064 pKa = 3.5QTILEE1069 pKa = 4.19GLEE1072 pKa = 4.04LDD1074 pKa = 4.05GTSMWRR1080 pKa = 11.84IEE1082 pKa = 4.2LGDD1085 pKa = 3.9NIRR1088 pKa = 11.84SGAHH1092 pKa = 3.63YY1093 pKa = 8.61TQFIVYY1099 pKa = 10.26DD1100 pKa = 4.12FDD1102 pKa = 5.33SDD1104 pKa = 3.78GKK1106 pKa = 11.17AEE1108 pKa = 4.27VALKK1112 pKa = 8.17TAPGTRR1118 pKa = 11.84DD1119 pKa = 3.04GEE1121 pKa = 4.5GNYY1124 pKa = 10.53LSTGPAADD1132 pKa = 4.94DD1133 pKa = 4.66DD1134 pKa = 4.68DD1135 pKa = 4.39AASYY1139 pKa = 7.72VTNTGRR1145 pKa = 11.84IIYY1148 pKa = 7.25PSPEE1152 pKa = 3.81YY1153 pKa = 10.23LTIFEE1158 pKa = 4.49GATGKK1163 pKa = 9.89EE1164 pKa = 4.29LQTEE1168 pKa = 5.14LYY1170 pKa = 10.17SPQYY1174 pKa = 10.6DD1175 pKa = 3.6FKK1177 pKa = 11.72NNPEE1181 pKa = 4.12NFWGDD1186 pKa = 3.38GYY1188 pKa = 11.15GNRR1191 pKa = 11.84SEE1193 pKa = 5.26RR1194 pKa = 11.84YY1195 pKa = 8.11LAAVGYY1201 pKa = 9.94FNGEE1205 pKa = 4.08SPSLVMSRR1213 pKa = 11.84GYY1215 pKa = 10.56YY1216 pKa = 9.13EE1217 pKa = 5.07GYY1219 pKa = 10.37VVAAWDD1225 pKa = 3.48WDD1227 pKa = 3.79GEE1229 pKa = 4.28NLTNRR1234 pKa = 11.84WNFEE1238 pKa = 4.03AGPWSSDD1245 pKa = 3.12PYY1247 pKa = 10.78GGQGNHH1253 pKa = 6.65NIAVGDD1259 pKa = 3.63VDD1261 pKa = 5.13EE1262 pKa = 5.46DD1263 pKa = 4.01GKK1265 pKa = 11.02DD1266 pKa = 3.33EE1267 pKa = 4.68IMFGSAAIDD1276 pKa = 3.71DD1277 pKa = 5.25DD1278 pKa = 4.23GTGLYY1283 pKa = 6.72TTRR1286 pKa = 11.84NGHH1289 pKa = 6.07GDD1291 pKa = 3.48AGHH1294 pKa = 7.36LGDD1297 pKa = 5.85LDD1299 pKa = 4.04PDD1301 pKa = 3.57IPGLEE1306 pKa = 3.68FFMPHH1311 pKa = 5.59EE1312 pKa = 4.12WSGPGLSFRR1321 pKa = 11.84NAGTGSMIWEE1331 pKa = 4.51VPSDD1335 pKa = 3.81DD1336 pKa = 4.02DD1337 pKa = 3.8VGRR1340 pKa = 11.84GVAEE1344 pKa = 5.27DD1345 pKa = 3.49ISAEE1349 pKa = 3.96YY1350 pKa = 10.22RR1351 pKa = 11.84GAEE1354 pKa = 3.71AWGLGAYY1361 pKa = 7.36NAKK1364 pKa = 10.08GDD1366 pKa = 4.92PIDD1369 pKa = 5.11LSPPSANFVIYY1380 pKa = 9.84WDD1382 pKa = 3.72GDD1384 pKa = 3.69VTRR1387 pKa = 11.84EE1388 pKa = 4.0LLDD1391 pKa = 3.48RR1392 pKa = 11.84NYY1394 pKa = 10.11IDD1396 pKa = 5.28DD1397 pKa = 4.22MEE1399 pKa = 4.64NGRR1402 pKa = 11.84IFTADD1407 pKa = 2.95GFSSNNGTKK1416 pKa = 8.93ATPNLSVDD1424 pKa = 4.19LFGDD1428 pKa = 2.98WRR1430 pKa = 11.84EE1431 pKa = 4.01EE1432 pKa = 4.07VIFRR1436 pKa = 11.84SDD1438 pKa = 3.59DD1439 pKa = 3.53NTSLRR1444 pKa = 11.84IFTTSDD1450 pKa = 2.69TSDD1453 pKa = 2.91VRR1455 pKa = 11.84RR1456 pKa = 11.84YY1457 pKa = 8.44TLMHH1461 pKa = 7.02DD1462 pKa = 3.27PLYY1465 pKa = 10.26RR1466 pKa = 11.84ISIAWQNVAYY1476 pKa = 9.28NQPPHH1481 pKa = 6.88VGYY1484 pKa = 10.93YY1485 pKa = 9.17MPDD1488 pKa = 3.46GSPQPNIIYY1497 pKa = 9.03PDD1499 pKa = 3.65EE1500 pKa = 4.26YY1501 pKa = 10.96VSVSINPEE1509 pKa = 3.84GKK1511 pKa = 9.86DD1512 pKa = 3.28GSVIKK1517 pKa = 10.22DD1518 pKa = 3.45YY1519 pKa = 11.3KK1520 pKa = 9.21LQNYY1524 pKa = 7.31PNPFNPTTNISFSLPVSGDD1543 pKa = 3.1VKK1545 pKa = 11.35LEE1547 pKa = 3.84VFDD1550 pKa = 3.89INGRR1554 pKa = 11.84LISTIVNEE1562 pKa = 4.18RR1563 pKa = 11.84MTSGTYY1569 pKa = 10.34SRR1571 pKa = 11.84TFDD1574 pKa = 3.57ASNLASGIYY1583 pKa = 10.11FYY1585 pKa = 11.31RR1586 pKa = 11.84LRR1588 pKa = 11.84TSDD1591 pKa = 3.41YY1592 pKa = 8.54TQVKK1596 pKa = 10.5KK1597 pKa = 10.82MMLIKK1602 pKa = 10.65

Molecular weight:
173.84 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A521CVQ6|A0A521CVQ6_9BACT Uncharacterized protein OS=Gracilimonas mengyeensis OX=1302730 GN=SAMN06265219_106133 PE=4 SV=1
MM1 pKa = 7.89ADD3 pKa = 3.27YY4 pKa = 10.97SHH6 pKa = 7.31RR7 pKa = 11.84FPIRR11 pKa = 11.84KK12 pKa = 7.63MSQVFGVSPSGYY24 pKa = 8.93YY25 pKa = 9.45RR26 pKa = 11.84WLGRR30 pKa = 11.84PPSKK34 pKa = 10.17RR35 pKa = 11.84ARR37 pKa = 11.84QNRR40 pKa = 11.84RR41 pKa = 11.84LKK43 pKa = 10.64EE44 pKa = 4.14SIRR47 pKa = 11.84LVWVLSKK54 pKa = 10.57RR55 pKa = 11.84IYY57 pKa = 9.75GAPRR61 pKa = 11.84IYY63 pKa = 10.42QEE65 pKa = 3.85LLRR68 pKa = 11.84RR69 pKa = 11.84GWQVSRR75 pKa = 11.84PRR77 pKa = 11.84VARR80 pKa = 11.84LMRR83 pKa = 11.84KK84 pKa = 8.53MGISSQLRR92 pKa = 11.84KK93 pKa = 10.0KK94 pKa = 8.34WVKK97 pKa = 7.05TTNSKK102 pKa = 10.26HH103 pKa = 4.48SHH105 pKa = 5.62PVAGNLLDD113 pKa = 4.61RR114 pKa = 11.84RR115 pKa = 11.84FTPTQLNQVWVSDD128 pKa = 3.38ITYY131 pKa = 10.32LPSQQGWLYY140 pKa = 9.11LTTVMDD146 pKa = 4.43LADD149 pKa = 4.09RR150 pKa = 11.84QILGWSLSTSMSAEE164 pKa = 3.93QTTTAAFCEE173 pKa = 4.32AHH175 pKa = 6.05TKK177 pKa = 10.22RR178 pKa = 11.84PARR181 pKa = 11.84KK182 pKa = 9.9GMIFHH187 pKa = 7.45SDD189 pKa = 2.44RR190 pKa = 11.84GMQYY194 pKa = 10.84ACSAFTNLLSKK205 pKa = 10.47HH206 pKa = 5.59QLIQSMSRR214 pKa = 11.84KK215 pKa = 9.17GNCWDD220 pKa = 3.56NAPAEE225 pKa = 4.59SFFKK229 pKa = 10.17TLKK232 pKa = 10.86AEE234 pKa = 4.28LVSQTGIFRR243 pKa = 11.84DD244 pKa = 3.93YY245 pKa = 10.17QQARR249 pKa = 11.84AAIFEE254 pKa = 4.64YY255 pKa = 10.6IEE257 pKa = 3.51GWYY260 pKa = 9.3NRR262 pKa = 11.84KK263 pKa = 9.23RR264 pKa = 11.84LHH266 pKa = 6.77SSLGYY271 pKa = 8.09KK272 pKa = 9.29TPAEE276 pKa = 4.33AEE278 pKa = 4.02QEE280 pKa = 4.22LKK282 pKa = 10.78QKK284 pKa = 10.3LQQAAA289 pKa = 3.29

Molecular weight:
33.6 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3810

0

3810

1339685

24

4477

351.6

39.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.06 ± 0.035

0.553 ± 0.01

6.057 ± 0.031

7.777 ± 0.047

4.72 ± 0.028

7.165 ± 0.036

1.933 ± 0.02

6.672 ± 0.033

5.607 ± 0.052

9.183 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.419 ± 0.021

4.858 ± 0.033

3.95 ± 0.022

3.829 ± 0.025

4.371 ± 0.03

6.648 ± 0.038

5.586 ± 0.043

6.499 ± 0.033

1.298 ± 0.017

3.816 ± 0.027

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski