Gracilimonas mengyeensis
Average proteome isoelectric point is 5.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3810 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A521C3D4|A0A521C3D4_9BACT Uncharacterized protein OS=Gracilimonas mengyeensis OX=1302730 GN=SAMN06265219_104120 PE=4 SV=1
MM1 pKa = 7.81 GIFPYY6 pKa = 10.55 AQAQEE11 pKa = 4.36 VVTTFDD17 pKa = 4.39 LNGADD22 pKa = 3.11 GTLYY26 pKa = 10.77 NDD28 pKa = 3.91 EE29 pKa = 4.29 NRR31 pKa = 11.84 GADD34 pKa = 3.62 FPGGDD39 pKa = 3.42 RR40 pKa = 11.84 SSLEE44 pKa = 3.14 IRR46 pKa = 11.84 YY47 pKa = 8.55 WDD49 pKa = 3.45 EE50 pKa = 3.6 VRR52 pKa = 11.84 FRR54 pKa = 11.84 ASMVRR59 pKa = 11.84 FDD61 pKa = 3.93 VNAVNSSPDD70 pKa = 3.41 GAVLGLYY77 pKa = 6.87 PTSAGSMGADD87 pKa = 2.53 SLTFTIYY94 pKa = 11.2 GLTNEE99 pKa = 5.67 SEE101 pKa = 4.7 DD102 pKa = 3.49 NWDD105 pKa = 3.74 EE106 pKa = 4.37 GSFSYY111 pKa = 11.3 NEE113 pKa = 3.94 APGFEE118 pKa = 4.04 AAANGTYY125 pKa = 10.29 AINEE129 pKa = 4.35 DD130 pKa = 4.4 LDD132 pKa = 3.9 SLTTITVYY140 pKa = 11.35 NNLTDD145 pKa = 3.44 TYY147 pKa = 10.66 LYY149 pKa = 10.47 SEE151 pKa = 5.19 PSAEE155 pKa = 4.21 LDD157 pKa = 3.42 AFINSDD163 pKa = 3.67 TNGLVTFAIVKK174 pKa = 8.7 TYY176 pKa = 8.63 STEE179 pKa = 3.77 SGSYY183 pKa = 9.91 VISPKK188 pKa = 10.36 EE189 pKa = 3.86 AGEE192 pKa = 4.28 SVAPKK197 pKa = 10.26 LVYY200 pKa = 10.12 QSEE203 pKa = 4.32 NGEE206 pKa = 3.88 PVLPNTEE213 pKa = 3.85 PSITLDD219 pKa = 3.53 SPTDD223 pKa = 3.59 GASYY227 pKa = 10.59 EE228 pKa = 4.52 SNNTLTASASASDD241 pKa = 3.82 SDD243 pKa = 4.64 GEE245 pKa = 4.11 IASVSFYY252 pKa = 11.17 LDD254 pKa = 3.19 DD255 pKa = 4.0 TEE257 pKa = 5.04 IGSVNASPFEE267 pKa = 4.0 YY268 pKa = 10.47 EE269 pKa = 4.31 IDD271 pKa = 3.41 LSMYY275 pKa = 11.01 ALGSHH280 pKa = 6.47 EE281 pKa = 4.84 FYY283 pKa = 10.85 AIAEE287 pKa = 4.53 DD288 pKa = 3.58 NDD290 pKa = 4.01 GAVASSDD297 pKa = 2.95 TVMFEE302 pKa = 3.34 ITEE305 pKa = 4.74 AIGGSDD311 pKa = 3.57 AEE313 pKa = 4.32 MVTTFEE319 pKa = 5.57 LNGADD324 pKa = 3.21 AAIYY328 pKa = 10.86 NDD330 pKa = 4.0 DD331 pKa = 4.2 NRR333 pKa = 11.84 GADD336 pKa = 3.46 YY337 pKa = 10.84 TGGNRR342 pKa = 11.84 SDD344 pKa = 3.97 LEE346 pKa = 3.55 IRR348 pKa = 11.84 YY349 pKa = 8.63 WEE351 pKa = 4.1 EE352 pKa = 3.3 VRR354 pKa = 11.84 FRR356 pKa = 11.84 ASILRR361 pKa = 11.84 FDD363 pKa = 4.01 VSNVSSTPNGAVIGFNPTYY382 pKa = 9.47 AGKK385 pKa = 9.24 MGAEE389 pKa = 4.06 SLTYY393 pKa = 9.73 TIYY396 pKa = 11.33 GMTDD400 pKa = 3.17 EE401 pKa = 5.82 SQDD404 pKa = 3.28 NWDD407 pKa = 4.08 EE408 pKa = 4.17 GSVNYY413 pKa = 10.46 SSAPGFEE420 pKa = 4.36 SAANGTYY427 pKa = 11.19 ALTEE431 pKa = 4.14 NMDD434 pKa = 3.96 SLTTITVYY442 pKa = 11.15 ADD444 pKa = 3.02 STGAFLYY451 pKa = 10.48 SDD453 pKa = 4.62 PSTEE457 pKa = 4.2 LDD459 pKa = 3.22 AFINNDD465 pKa = 3.25 SNGLVTFAIIKK476 pKa = 8.02 TISTNSDD483 pKa = 3.64 YY484 pKa = 11.66 YY485 pKa = 11.89 AMNSKK490 pKa = 10.02 EE491 pKa = 4.18 AGVSVAPRR499 pKa = 11.84 LVYY502 pKa = 10.33 QSEE505 pKa = 4.15 NGEE508 pKa = 3.83 PVLPNFEE515 pKa = 4.23 PSITLNSPEE524 pKa = 4.31 EE525 pKa = 4.11 GMVYY529 pKa = 10.43 QSDD532 pKa = 3.83 STLTARR538 pKa = 11.84 ATAIDD543 pKa = 3.86 SDD545 pKa = 4.59 GEE547 pKa = 4.04 IASVSFYY554 pKa = 10.55 MDD556 pKa = 3.52 GTAVANVTSSPYY568 pKa = 10.15 EE569 pKa = 4.01 SEE571 pKa = 4.32 IDD573 pKa = 3.36 LSMYY577 pKa = 11.03 ASGSYY582 pKa = 9.67 EE583 pKa = 3.93 FYY585 pKa = 10.83 AIAEE589 pKa = 4.59 DD590 pKa = 4.87 DD591 pKa = 4.46 DD592 pKa = 4.59 GATASSDD599 pKa = 3.25 TVTIDD604 pKa = 2.94 IVGGSGAGSSEE615 pKa = 3.94 MVTTFDD621 pKa = 4.66 SNGADD626 pKa = 2.97 VTLFNDD632 pKa = 3.55 EE633 pKa = 3.94 NRR635 pKa = 11.84 AGSFTGGDD643 pKa = 3.23 RR644 pKa = 11.84 TDD646 pKa = 3.56 LEE648 pKa = 4.06 VRR650 pKa = 11.84 YY651 pKa = 9.19 WEE653 pKa = 4.03 AVRR656 pKa = 11.84 FRR658 pKa = 11.84 ASMLRR663 pKa = 11.84 FDD665 pKa = 4.29 VSSVSAAPDD674 pKa = 3.46 GAVIGINPTYY684 pKa = 10.4 AEE686 pKa = 4.07 NMSDD690 pKa = 3.29 EE691 pKa = 4.5 TLSFTIFGLTNEE703 pKa = 5.5 AEE705 pKa = 4.42 DD706 pKa = 3.78 EE707 pKa = 3.99 WDD709 pKa = 3.72 EE710 pKa = 4.8 GSISYY715 pKa = 10.81 NSAPGFEE722 pKa = 4.45 SAINGSYY729 pKa = 10.59 AINDD733 pKa = 4.15 DD734 pKa = 4.36 LDD736 pKa = 3.82 SLTTITVYY744 pKa = 11.14 ADD746 pKa = 3.13 STGTFLYY753 pKa = 10.57 SEE755 pKa = 4.96 PSDD758 pKa = 3.75 KK759 pKa = 11.07 LDD761 pKa = 3.72 AFIRR765 pKa = 11.84 NDD767 pKa = 3.23 NNGLVTFAIIRR778 pKa = 11.84 TYY780 pKa = 11.43 SNNSDD785 pKa = 3.18 IYY787 pKa = 11.42 AMSSKK792 pKa = 10.42 EE793 pKa = 3.87 AGEE796 pKa = 4.01 LVAPRR801 pKa = 11.84 LVYY804 pKa = 10.38 KK805 pKa = 10.5 SEE807 pKa = 4.04 NGVPVIPNLEE817 pKa = 4.1 PNITLSRR824 pKa = 11.84 PADD827 pKa = 3.57 GVSYY831 pKa = 10.26 EE832 pKa = 4.36 SNSMLTALASASDD845 pKa = 4.17 PDD847 pKa = 4.19 GEE849 pKa = 4.16 IANVSFYY856 pKa = 10.52 MDD858 pKa = 3.44 GNEE861 pKa = 4.21 IGSATSSPYY870 pKa = 10.23 EE871 pKa = 3.91 YY872 pKa = 10.44 EE873 pKa = 3.89 IDD875 pKa = 3.54 LSIYY879 pKa = 10.27 EE880 pKa = 4.15 LGSYY884 pKa = 9.91 EE885 pKa = 4.06 FFAIAEE891 pKa = 4.52 DD892 pKa = 4.29 DD893 pKa = 4.32 DD894 pKa = 4.58 GALASSDD901 pKa = 3.37 TVTFEE906 pKa = 4.36 IIEE909 pKa = 4.41 GNGGGSEE916 pKa = 4.03 LPARR920 pKa = 11.84 VMEE923 pKa = 5.34 DD924 pKa = 2.7 IDD926 pKa = 4.26 RR927 pKa = 11.84 GLIAIDD933 pKa = 4.03 RR934 pKa = 11.84 GDD936 pKa = 4.98 DD937 pKa = 3.4 IYY939 pKa = 11.52 LSWRR943 pKa = 11.84 LLGKK947 pKa = 8.89 EE948 pKa = 3.98 APDD951 pKa = 3.59 LGFNIYY957 pKa = 10.22 RR958 pKa = 11.84 DD959 pKa = 3.69 GTLVNSEE966 pKa = 5.0 PITDD970 pKa = 3.44 RR971 pKa = 11.84 TNYY974 pKa = 9.86 VDD976 pKa = 4.6 ADD978 pKa = 3.76 GSADD982 pKa = 3.38 ANYY985 pKa = 10.9 SVIPVEE991 pKa = 5.27 DD992 pKa = 3.63 GTEE995 pKa = 3.97 LSEE998 pKa = 4.6 SEE1000 pKa = 3.99 ATASVWANSFIDD1012 pKa = 3.42 IPLNIPADD1020 pKa = 3.93 GTHH1023 pKa = 6.4 GGSYY1027 pKa = 10.31 SPNDD1031 pKa = 3.59 MSIGDD1036 pKa = 4.07 LDD1038 pKa = 3.95 GDD1040 pKa = 3.88 NEE1042 pKa = 4.34 YY1043 pKa = 11.23 EE1044 pKa = 5.87 LVLKK1048 pKa = 9.36 WYY1050 pKa = 9.32 PSNAKK1055 pKa = 10.5 DD1056 pKa = 3.52 NAQDD1060 pKa = 3.49 GLTDD1064 pKa = 3.5 QTILEE1069 pKa = 4.19 GLEE1072 pKa = 4.04 LDD1074 pKa = 4.05 GTSMWRR1080 pKa = 11.84 IEE1082 pKa = 4.2 LGDD1085 pKa = 3.9 NIRR1088 pKa = 11.84 SGAHH1092 pKa = 3.63 YY1093 pKa = 8.61 TQFIVYY1099 pKa = 10.26 DD1100 pKa = 4.12 FDD1102 pKa = 5.33 SDD1104 pKa = 3.78 GKK1106 pKa = 11.17 AEE1108 pKa = 4.27 VALKK1112 pKa = 8.17 TAPGTRR1118 pKa = 11.84 DD1119 pKa = 3.04 GEE1121 pKa = 4.5 GNYY1124 pKa = 10.53 LSTGPAADD1132 pKa = 4.94 DD1133 pKa = 4.66 DD1134 pKa = 4.68 DD1135 pKa = 4.39 AASYY1139 pKa = 7.72 VTNTGRR1145 pKa = 11.84 IIYY1148 pKa = 7.25 PSPEE1152 pKa = 3.81 YY1153 pKa = 10.23 LTIFEE1158 pKa = 4.49 GATGKK1163 pKa = 9.89 EE1164 pKa = 4.29 LQTEE1168 pKa = 5.14 LYY1170 pKa = 10.17 SPQYY1174 pKa = 10.6 DD1175 pKa = 3.6 FKK1177 pKa = 11.72 NNPEE1181 pKa = 4.12 NFWGDD1186 pKa = 3.38 GYY1188 pKa = 11.15 GNRR1191 pKa = 11.84 SEE1193 pKa = 5.26 RR1194 pKa = 11.84 YY1195 pKa = 8.11 LAAVGYY1201 pKa = 9.94 FNGEE1205 pKa = 4.08 SPSLVMSRR1213 pKa = 11.84 GYY1215 pKa = 10.56 YY1216 pKa = 9.13 EE1217 pKa = 5.07 GYY1219 pKa = 10.37 VVAAWDD1225 pKa = 3.48 WDD1227 pKa = 3.79 GEE1229 pKa = 4.28 NLTNRR1234 pKa = 11.84 WNFEE1238 pKa = 4.03 AGPWSSDD1245 pKa = 3.12 PYY1247 pKa = 10.78 GGQGNHH1253 pKa = 6.65 NIAVGDD1259 pKa = 3.63 VDD1261 pKa = 5.13 EE1262 pKa = 5.46 DD1263 pKa = 4.01 GKK1265 pKa = 11.02 DD1266 pKa = 3.33 EE1267 pKa = 4.68 IMFGSAAIDD1276 pKa = 3.71 DD1277 pKa = 5.25 DD1278 pKa = 4.23 GTGLYY1283 pKa = 6.72 TTRR1286 pKa = 11.84 NGHH1289 pKa = 6.07 GDD1291 pKa = 3.48 AGHH1294 pKa = 7.36 LGDD1297 pKa = 5.85 LDD1299 pKa = 4.04 PDD1301 pKa = 3.57 IPGLEE1306 pKa = 3.68 FFMPHH1311 pKa = 5.59 EE1312 pKa = 4.12 WSGPGLSFRR1321 pKa = 11.84 NAGTGSMIWEE1331 pKa = 4.51 VPSDD1335 pKa = 3.81 DD1336 pKa = 4.02 DD1337 pKa = 3.8 VGRR1340 pKa = 11.84 GVAEE1344 pKa = 5.27 DD1345 pKa = 3.49 ISAEE1349 pKa = 3.96 YY1350 pKa = 10.22 RR1351 pKa = 11.84 GAEE1354 pKa = 3.71 AWGLGAYY1361 pKa = 7.36 NAKK1364 pKa = 10.08 GDD1366 pKa = 4.92 PIDD1369 pKa = 5.11 LSPPSANFVIYY1380 pKa = 9.84 WDD1382 pKa = 3.72 GDD1384 pKa = 3.69 VTRR1387 pKa = 11.84 EE1388 pKa = 4.0 LLDD1391 pKa = 3.48 RR1392 pKa = 11.84 NYY1394 pKa = 10.11 IDD1396 pKa = 5.28 DD1397 pKa = 4.22 MEE1399 pKa = 4.64 NGRR1402 pKa = 11.84 IFTADD1407 pKa = 2.95 GFSSNNGTKK1416 pKa = 8.93 ATPNLSVDD1424 pKa = 4.19 LFGDD1428 pKa = 2.98 WRR1430 pKa = 11.84 EE1431 pKa = 4.01 EE1432 pKa = 4.07 VIFRR1436 pKa = 11.84 SDD1438 pKa = 3.59 DD1439 pKa = 3.53 NTSLRR1444 pKa = 11.84 IFTTSDD1450 pKa = 2.69 TSDD1453 pKa = 2.91 VRR1455 pKa = 11.84 RR1456 pKa = 11.84 YY1457 pKa = 8.44 TLMHH1461 pKa = 7.02 DD1462 pKa = 3.27 PLYY1465 pKa = 10.26 RR1466 pKa = 11.84 ISIAWQNVAYY1476 pKa = 9.28 NQPPHH1481 pKa = 6.88 VGYY1484 pKa = 10.93 YY1485 pKa = 9.17 MPDD1488 pKa = 3.46 GSPQPNIIYY1497 pKa = 9.03 PDD1499 pKa = 3.65 EE1500 pKa = 4.26 YY1501 pKa = 10.96 VSVSINPEE1509 pKa = 3.84 GKK1511 pKa = 9.86 DD1512 pKa = 3.28 GSVIKK1517 pKa = 10.22 DD1518 pKa = 3.45 YY1519 pKa = 11.3 KK1520 pKa = 9.21 LQNYY1524 pKa = 7.31 PNPFNPTTNISFSLPVSGDD1543 pKa = 3.1 VKK1545 pKa = 11.35 LEE1547 pKa = 3.84 VFDD1550 pKa = 3.89 INGRR1554 pKa = 11.84 LISTIVNEE1562 pKa = 4.18 RR1563 pKa = 11.84 MTSGTYY1569 pKa = 10.34 SRR1571 pKa = 11.84 TFDD1574 pKa = 3.57 ASNLASGIYY1583 pKa = 10.11 FYY1585 pKa = 11.31 RR1586 pKa = 11.84 LRR1588 pKa = 11.84 TSDD1591 pKa = 3.41 YY1592 pKa = 8.54 TQVKK1596 pKa = 10.5 KK1597 pKa = 10.82 MMLIKK1602 pKa = 10.65
Molecular weight: 173.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.697
IPC2_protein 3.745
IPC_protein 3.783
Toseland 3.567
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.681
Rodwell 3.605
Grimsley 3.465
Solomon 3.77
Lehninger 3.719
Nozaki 3.872
DTASelect 4.113
Thurlkill 3.605
EMBOSS 3.694
Sillero 3.897
Patrickios 0.998
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.802
Protein with the highest isoelectric point:
>tr|A0A521CVQ6|A0A521CVQ6_9BACT Uncharacterized protein OS=Gracilimonas mengyeensis OX=1302730 GN=SAMN06265219_106133 PE=4 SV=1
MM1 pKa = 7.89 ADD3 pKa = 3.27 YY4 pKa = 10.97 SHH6 pKa = 7.31 RR7 pKa = 11.84 FPIRR11 pKa = 11.84 KK12 pKa = 7.63 MSQVFGVSPSGYY24 pKa = 8.93 YY25 pKa = 9.45 RR26 pKa = 11.84 WLGRR30 pKa = 11.84 PPSKK34 pKa = 10.17 RR35 pKa = 11.84 ARR37 pKa = 11.84 QNRR40 pKa = 11.84 RR41 pKa = 11.84 LKK43 pKa = 10.64 EE44 pKa = 4.14 SIRR47 pKa = 11.84 LVWVLSKK54 pKa = 10.57 RR55 pKa = 11.84 IYY57 pKa = 9.75 GAPRR61 pKa = 11.84 IYY63 pKa = 10.42 QEE65 pKa = 3.85 LLRR68 pKa = 11.84 RR69 pKa = 11.84 GWQVSRR75 pKa = 11.84 PRR77 pKa = 11.84 VARR80 pKa = 11.84 LMRR83 pKa = 11.84 KK84 pKa = 8.53 MGISSQLRR92 pKa = 11.84 KK93 pKa = 10.0 KK94 pKa = 8.34 WVKK97 pKa = 7.05 TTNSKK102 pKa = 10.26 HH103 pKa = 4.48 SHH105 pKa = 5.62 PVAGNLLDD113 pKa = 4.61 RR114 pKa = 11.84 RR115 pKa = 11.84 FTPTQLNQVWVSDD128 pKa = 3.38 ITYY131 pKa = 10.32 LPSQQGWLYY140 pKa = 9.11 LTTVMDD146 pKa = 4.43 LADD149 pKa = 4.09 RR150 pKa = 11.84 QILGWSLSTSMSAEE164 pKa = 3.93 QTTTAAFCEE173 pKa = 4.32 AHH175 pKa = 6.05 TKK177 pKa = 10.22 RR178 pKa = 11.84 PARR181 pKa = 11.84 KK182 pKa = 9.9 GMIFHH187 pKa = 7.45 SDD189 pKa = 2.44 RR190 pKa = 11.84 GMQYY194 pKa = 10.84 ACSAFTNLLSKK205 pKa = 10.47 HH206 pKa = 5.59 QLIQSMSRR214 pKa = 11.84 KK215 pKa = 9.17 GNCWDD220 pKa = 3.56 NAPAEE225 pKa = 4.59 SFFKK229 pKa = 10.17 TLKK232 pKa = 10.86 AEE234 pKa = 4.28 LVSQTGIFRR243 pKa = 11.84 DD244 pKa = 3.93 YY245 pKa = 10.17 QQARR249 pKa = 11.84 AAIFEE254 pKa = 4.64 YY255 pKa = 10.6 IEE257 pKa = 3.51 GWYY260 pKa = 9.3 NRR262 pKa = 11.84 KK263 pKa = 9.23 RR264 pKa = 11.84 LHH266 pKa = 6.77 SSLGYY271 pKa = 8.09 KK272 pKa = 9.29 TPAEE276 pKa = 4.33 AEE278 pKa = 4.02 QEE280 pKa = 4.22 LKK282 pKa = 10.78 QKK284 pKa = 10.3 LQQAAA289 pKa = 3.29
Molecular weight: 33.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.327
IPC2_protein 9.897
IPC_protein 10.657
Toseland 10.73
ProMoST 10.423
Dawson 10.833
Bjellqvist 10.54
Wikipedia 11.038
Rodwell 11.111
Grimsley 10.891
Solomon 10.935
Lehninger 10.891
Nozaki 10.701
DTASelect 10.54
Thurlkill 10.73
EMBOSS 11.125
Sillero 10.76
Patrickios 10.804
IPC_peptide 10.935
IPC2_peptide 9.472
IPC2.peptide.svr19 8.427
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3810
0
3810
1339685
24
4477
351.6
39.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.06 ± 0.035
0.553 ± 0.01
6.057 ± 0.031
7.777 ± 0.047
4.72 ± 0.028
7.165 ± 0.036
1.933 ± 0.02
6.672 ± 0.033
5.607 ± 0.052
9.183 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.419 ± 0.021
4.858 ± 0.033
3.95 ± 0.022
3.829 ± 0.025
4.371 ± 0.03
6.648 ± 0.038
5.586 ± 0.043
6.499 ± 0.033
1.298 ± 0.017
3.816 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here