Priestia koreensis
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4402 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0M0KWW1|A0A0M0KWW1_9BACI FeoA domain-containing protein OS=Priestia koreensis OX=284581 GN=AMD01_17175 PE=4 SV=1
MM1 pKa = 7.81 EE2 pKa = 4.64 YY3 pKa = 10.77 SGMASVWFGVSKK15 pKa = 10.84 SFEE18 pKa = 4.06 NLKK21 pKa = 10.44 EE22 pKa = 4.08 YY23 pKa = 11.32 VDD25 pKa = 3.73 IEE27 pKa = 4.25 YY28 pKa = 10.22 TEE30 pKa = 5.35 DD31 pKa = 3.67 GDD33 pKa = 5.61 SIDD36 pKa = 4.93 SKK38 pKa = 11.14 FGTNFEE44 pKa = 4.01 FGYY47 pKa = 10.51 YY48 pKa = 10.33 DD49 pKa = 3.61 EE50 pKa = 6.29 DD51 pKa = 4.18 NIEE54 pKa = 3.67 ICFYY58 pKa = 10.9 EE59 pKa = 4.2 NPKK62 pKa = 10.99 NNVDD66 pKa = 5.2 DD67 pKa = 4.01 ILNDD71 pKa = 3.8 FSYY74 pKa = 11.27 SEE76 pKa = 4.84 LIIPKK81 pKa = 9.76 IKK83 pKa = 10.43 EE84 pKa = 4.26 LINGDD89 pKa = 3.38 KK90 pKa = 10.46 LAYY93 pKa = 9.97 SINSVIVLYY102 pKa = 10.56 DD103 pKa = 3.31 FQYY106 pKa = 11.56 NEE108 pKa = 4.41 AKK110 pKa = 10.71 SGDD113 pKa = 3.55 EE114 pKa = 4.31 SEE116 pKa = 4.47 NIEE119 pKa = 3.87 IKK121 pKa = 10.85 FIGTVPYY128 pKa = 10.26 KK129 pKa = 10.86
Molecular weight: 14.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.771
IPC2_protein 3.986
IPC_protein 3.923
Toseland 3.732
ProMoST 4.062
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.795
Rodwell 3.757
Grimsley 3.643
Solomon 3.884
Lehninger 3.834
Nozaki 4.012
DTASelect 4.177
Thurlkill 3.77
EMBOSS 3.808
Sillero 4.037
Patrickios 1.036
IPC_peptide 3.884
IPC2_peptide 4.024
IPC2.peptide.svr19 3.937
Protein with the highest isoelectric point:
>tr|A0A0M0L5N4|A0A0M0L5N4_9BACI Uncharacterized protein OS=Priestia koreensis OX=284581 GN=AMD01_11135 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.34 QPNKK9 pKa = 8.21 RR10 pKa = 11.84 KK11 pKa = 9.62 RR12 pKa = 11.84 SKK14 pKa = 9.59 VHH16 pKa = 5.83 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MSSANGRR28 pKa = 11.84 KK29 pKa = 8.82 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.05 GRR39 pKa = 11.84 KK40 pKa = 8.7 VLSAA44 pKa = 4.05
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.398
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.135
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.06
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4402
0
4402
1238965
26
4886
281.5
31.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.991 ± 0.038
0.71 ± 0.012
5.082 ± 0.029
7.259 ± 0.051
4.459 ± 0.029
6.769 ± 0.036
2.334 ± 0.021
7.334 ± 0.044
6.813 ± 0.041
9.829 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.76 ± 0.02
4.112 ± 0.035
3.594 ± 0.026
4.019 ± 0.026
4.101 ± 0.029
6.174 ± 0.031
5.64 ± 0.048
7.363 ± 0.031
1.031 ± 0.013
3.626 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here