Orrella dioscoreae
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4379 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1C3K1F8|A0A1C3K1F8_9BURK Phage protein OS=Orrella dioscoreae OX=1851544 GN=ODI_03639 PE=4 SV=1
MM1 pKa = 7.97 DD2 pKa = 4.87 EE3 pKa = 4.25 EE4 pKa = 5.19 LGASGRR10 pKa = 11.84 QYY12 pKa = 11.81 DD13 pKa = 4.45 LTDD16 pKa = 3.94 GPAFSNEE23 pKa = 3.89 TGYY26 pKa = 10.58 HH27 pKa = 4.07 VHH29 pKa = 5.73 VVPMDD34 pKa = 4.15 LDD36 pKa = 3.39 IADD39 pKa = 4.47 GQDD42 pKa = 2.99 EE43 pKa = 4.3 DD44 pKa = 4.32 VIEE47 pKa = 4.4 VVSALPYY54 pKa = 9.63 VGYY57 pKa = 10.8 LRR59 pKa = 11.84 VTASAA64 pKa = 3.87
Molecular weight: 6.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.795
IPC_protein 3.706
Toseland 3.516
ProMoST 3.91
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.681
Rodwell 3.554
Grimsley 3.439
Solomon 3.694
Lehninger 3.643
Nozaki 3.859
DTASelect 4.062
Thurlkill 3.592
EMBOSS 3.694
Sillero 3.846
Patrickios 0.693
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.755
Protein with the highest isoelectric point:
>tr|A0A1C3K6W2|A0A1C3K6W2_9BURK Uncharacterized protein OS=Orrella dioscoreae OX=1851544 GN=ODI_03820 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVTRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.0 RR14 pKa = 11.84 THH16 pKa = 5.76 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MKK23 pKa = 9.24 TRR25 pKa = 11.84 AGRR28 pKa = 11.84 AIISARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.88 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.705
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.427
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.21
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4379
0
4379
1450914
37
3709
331.3
35.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.471 ± 0.054
0.838 ± 0.011
5.466 ± 0.029
4.966 ± 0.032
3.233 ± 0.023
8.618 ± 0.043
2.18 ± 0.021
4.211 ± 0.027
2.571 ± 0.03
11.085 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.316 ± 0.017
2.388 ± 0.02
5.483 ± 0.029
4.024 ± 0.026
7.433 ± 0.037
5.234 ± 0.027
5.129 ± 0.029
7.613 ± 0.03
1.459 ± 0.017
2.282 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here