Sulfuriferula sp. AH1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Sulfuricellaceae; Sulfuriferula; unclassified Sulfuriferula

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2803 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y0GA42|A0A1Y0GA42_9PROT HTH lysR-type domain-containing protein OS=Sulfuriferula sp. AH1 OX=1985873 GN=CAP31_11405 PE=3 SV=1
MM1 pKa = 7.31NAMTEE6 pKa = 4.23MPSVLVFTDD15 pKa = 3.27NAANKK20 pKa = 8.43VAQLIEE26 pKa = 4.38EE27 pKa = 4.51EE28 pKa = 4.63GNPDD32 pKa = 2.85LKK34 pKa = 11.17LRR36 pKa = 11.84VFVSGGGCSGFQYY49 pKa = 10.9GFTFDD54 pKa = 3.8EE55 pKa = 4.88VVNEE59 pKa = 4.97DD60 pKa = 3.62DD61 pKa = 3.28TSMVKK66 pKa = 10.61NGVTLLIDD74 pKa = 3.7PMSYY78 pKa = 10.31QYY80 pKa = 11.58LVGAEE85 pKa = 3.69IDD87 pKa = 3.85YY88 pKa = 11.42QEE90 pKa = 4.6GLEE93 pKa = 4.17GAQFVIKK100 pKa = 10.53NPNATSTCGCGSSFSAA116 pKa = 4.79

Molecular weight: 12.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y0GAQ0|A0A1Y0GAQ0_9PROT Uncharacterized protein OS=Sulfuriferula sp. AH1 OX=1985873 GN=CAP31_00840 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 9.6QPSVIRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.64RR14 pKa = 11.84THH16 pKa = 5.96GFLVRR21 pKa = 11.84MRR23 pKa = 11.84TKK25 pKa = 10.6GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.3GRR39 pKa = 11.84VRR41 pKa = 11.84LAVGEE46 pKa = 4.31SRR48 pKa = 4.63

Molecular weight: 5.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2803

0

2803

864869

37

1898

308.6

33.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.185 ± 0.066

0.928 ± 0.016

5.385 ± 0.03

5.39 ± 0.045

3.627 ± 0.029

7.372 ± 0.045

2.54 ± 0.025

5.854 ± 0.039

3.976 ± 0.047

10.728 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.767 ± 0.022

3.491 ± 0.032

4.595 ± 0.033

4.385 ± 0.034

5.866 ± 0.04

5.477 ± 0.032

5.337 ± 0.041

7.056 ± 0.043

1.297 ± 0.02

2.744 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski