[Clostridium] cocleatum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Erysipelotrichia; Erysipelotrichales; Erysipelotrichaceae; Erysipelatoclostridium

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2756 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I0BK92|A0A1I0BK92_9FIRM Uncharacterized protein OS=[Clostridium] cocleatum OX=69824 GN=SAMN04489758_101159 PE=4 SV=1
MM1 pKa = 7.53NDD3 pKa = 2.91VYY5 pKa = 11.29DD6 pKa = 4.94FKK8 pKa = 11.36SEE10 pKa = 4.06EE11 pKa = 4.08LTQEE15 pKa = 3.68ILFEE19 pKa = 4.41NKK21 pKa = 10.0ADD23 pKa = 3.99FLISLSKK30 pKa = 11.1LCDD33 pKa = 3.38NLRR36 pKa = 11.84KK37 pKa = 10.0YY38 pKa = 10.63EE39 pKa = 4.11FVAIYY44 pKa = 6.86TTNEE48 pKa = 3.51FTKK51 pKa = 10.11WLLEE55 pKa = 4.22TYY57 pKa = 10.12DD58 pKa = 3.61IEE60 pKa = 4.7VDD62 pKa = 3.42EE63 pKa = 5.8LYY65 pKa = 10.93SEE67 pKa = 5.5DD68 pKa = 4.29DD69 pKa = 3.6FCIVTIAYY77 pKa = 8.65DD78 pKa = 3.39GNIIVEE84 pKa = 4.25PTVNDD89 pKa = 3.72NIITLSSATLTIFDD103 pKa = 4.22ATCPTRR109 pKa = 11.84FLKK112 pKa = 10.77ALEE115 pKa = 4.13NNEE118 pKa = 4.08EE119 pKa = 4.21NILIYY124 pKa = 10.73DD125 pKa = 4.06FEE127 pKa = 6.29KK128 pKa = 11.05EE129 pKa = 3.98LL130 pKa = 4.25

Molecular weight: 15.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I0HA82|A0A1I0HA82_9FIRM Predicted dehydrogenase OS=[Clostridium] cocleatum OX=69824 GN=SAMN04489758_14612 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.51RR10 pKa = 11.84KK11 pKa = 9.77RR12 pKa = 11.84SKK14 pKa = 8.62THH16 pKa = 5.5GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.08VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.02RR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.93VLSAA44 pKa = 4.11

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2756

0

2756

839769

30

2199

304.7

34.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.541 ± 0.051

1.294 ± 0.023

6.149 ± 0.047

6.836 ± 0.05

4.318 ± 0.04

5.888 ± 0.048

1.617 ± 0.021

9.509 ± 0.065

8.434 ± 0.056

9.163 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.632 ± 0.025

6.614 ± 0.06

2.618 ± 0.021

3.068 ± 0.027

3.199 ± 0.032

5.972 ± 0.039

5.137 ± 0.04

6.435 ± 0.042

0.734 ± 0.016

4.842 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski