Corallincola sp. C4
Average proteome isoelectric point is 6.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3747 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A368NJE5|A0A368NJE5_9GAMM Hotdog fold thioesterase OS=Corallincola sp. C4 OX=2282215 GN=DU002_10280 PE=4 SV=1
MM1 pKa = 7.82 RR2 pKa = 11.84 KK3 pKa = 10.1 LIITLAVASALAVTGCDD20 pKa = 5.25 DD21 pKa = 4.69 EE22 pKa = 5.73 SLDD25 pKa = 5.24 DD26 pKa = 4.69 ARR28 pKa = 11.84 NDD30 pKa = 3.58 AAGTPVINASKK41 pKa = 10.35 ISYY44 pKa = 10.23 DD45 pKa = 3.48 PANGVLPVPNDD56 pKa = 3.67 LLYY59 pKa = 10.69 TDD61 pKa = 4.24 TTDD64 pKa = 3.02 GTLNIPVTDD73 pKa = 4.67 PSDD76 pKa = 3.42 LSNPQVAINALDD88 pKa = 3.51 GWSTNQPFVINVITDD103 pKa = 3.31 GDD105 pKa = 4.06 VTLDD109 pKa = 3.82 PASAAAPGVVRR120 pKa = 11.84 IYY122 pKa = 9.22 EE123 pKa = 4.38 TVFGGPLAPPEE134 pKa = 4.26 CAEE137 pKa = 4.27 VPTGAACMVIAEE149 pKa = 4.78 LAFGTDD155 pKa = 5.05 FITSSSGNQLLIMPLAPLKK174 pKa = 10.99 AKK176 pKa = 9.37 TGYY179 pKa = 10.93 LITTTSLLMDD189 pKa = 3.82 SEE191 pKa = 4.46 SRR193 pKa = 11.84 PVQASSTYY201 pKa = 9.99 TLVKK205 pKa = 10.24 QDD207 pKa = 3.99 INTLPLGTPSQLLLQGAVNSYY228 pKa = 10.11 EE229 pKa = 3.85 NAIAAFGVDD238 pKa = 3.48 TSVITYY244 pKa = 9.49 SGAMTTQSIEE254 pKa = 4.18 DD255 pKa = 3.47 VSALNRR261 pKa = 11.84 LTMAADD267 pKa = 3.77 PATTPVLMPPAMTGLTVADD286 pKa = 4.08 ALYY289 pKa = 10.65 EE290 pKa = 4.07 LGIRR294 pKa = 11.84 DD295 pKa = 4.22 TNTLAVASTALLANSTLTNVPYY317 pKa = 10.69 YY318 pKa = 11.04 LEE320 pKa = 4.51 FPNFGNCDD328 pKa = 3.55 LTEE331 pKa = 4.29 LATTSQCDD339 pKa = 3.64 AINSHH344 pKa = 5.96 WLAAGDD350 pKa = 4.07 SPVTILGAVQSGALPPEE367 pKa = 4.48 AVLASCPDD375 pKa = 3.67 ADD377 pKa = 4.62 LSNAASLVGCEE388 pKa = 4.3 IKK390 pKa = 10.63 DD391 pKa = 3.7 AEE393 pKa = 4.49 GNPIGLDD400 pKa = 3.3 PEE402 pKa = 4.78 RR403 pKa = 11.84 NLTKK407 pKa = 10.5 YY408 pKa = 10.64 NPLAAPTEE416 pKa = 4.21 MQTLDD421 pKa = 3.68 VLVTLPDD428 pKa = 3.3 TSDD431 pKa = 3.06 AANGVRR437 pKa = 11.84 AALGLPAITAAPAAGWPVVVFSHH460 pKa = 7.18 GIGGAKK466 pKa = 8.83 EE467 pKa = 3.88 QNLALAGSFALQGLAMVSIDD487 pKa = 3.88 APLHH491 pKa = 5.84 GSRR494 pKa = 11.84 GWDD497 pKa = 2.78 ADD499 pKa = 3.62 MNGVFEE505 pKa = 4.87 ISASSGMIAFDD516 pKa = 3.42 PVKK519 pKa = 10.39 YY520 pKa = 10.88 ANANVLLYY528 pKa = 11.0 VKK530 pKa = 10.37 LDD532 pKa = 3.61 NLLTTRR538 pKa = 11.84 DD539 pKa = 3.53 NFRR542 pKa = 11.84 QNINDD547 pKa = 3.73 HH548 pKa = 6.17 LALRR552 pKa = 11.84 MGFNSLAQVAPGVFDD567 pKa = 3.58 PTRR570 pKa = 11.84 VSISGVSLGGINTVSTAAVGGLPLIDD596 pKa = 3.86 PTTGSEE602 pKa = 4.19 LPSYY606 pKa = 10.24 YY607 pKa = 10.72 AFSTVMPNVPAQGLAGVFAYY627 pKa = 10.47 SEE629 pKa = 4.43 TFGPVAKK636 pKa = 10.27 AAFVEE641 pKa = 4.35 SDD643 pKa = 3.31 SFKK646 pKa = 11.0 QVLSDD651 pKa = 3.46 ATGLSLEE658 pKa = 4.22 EE659 pKa = 4.01 LAALEE664 pKa = 4.82 ASDD667 pKa = 3.97 PDD669 pKa = 3.86 AYY671 pKa = 10.48 QALVDD676 pKa = 3.67 QVYY679 pKa = 8.14 PTFIAQFVFAAQTVVDD695 pKa = 3.87 SSDD698 pKa = 3.44 PVGYY702 pKa = 10.77 APILAGQGVPVYY714 pKa = 9.94 LAEE717 pKa = 4.31 VVGDD721 pKa = 3.63 TVLPNDD727 pKa = 3.52 NSAFGLPLSGTEE739 pKa = 3.61 PLIRR743 pKa = 11.84 VLGLPGVDD751 pKa = 3.14 ATVTGAPATGAVRR764 pKa = 11.84 FIDD767 pKa = 3.78 SAHH770 pKa = 6.54 GSVADD775 pKa = 3.72 PTVNPAATVEE785 pKa = 4.31 MQTQLAVYY793 pKa = 8.5 AASGGAMILVSDD805 pKa = 4.59 PSVVQPVMM813 pKa = 3.63
Molecular weight: 83.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.721
IPC2_protein 3.795
IPC_protein 3.834
Toseland 3.605
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.77
Rodwell 3.656
Grimsley 3.516
Solomon 3.834
Lehninger 3.783
Nozaki 3.935
DTASelect 4.202
Thurlkill 3.656
EMBOSS 3.783
Sillero 3.961
Patrickios 1.901
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.853
Protein with the highest isoelectric point:
>tr|A0A368N695|A0A368N695_9GAMM Erythrose-4-phosphate dehydrogenase OS=Corallincola sp. C4 OX=2282215 GN=DU002_14455 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.42 RR12 pKa = 11.84 KK13 pKa = 9.36 RR14 pKa = 11.84 SHH16 pKa = 6.15 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.38 NGRR28 pKa = 11.84 ALLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.41 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSAA44 pKa = 3.91
Molecular weight: 5.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3747
0
3747
1271560
37
11921
339.4
37.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.595 ± 0.043
1.079 ± 0.018
5.745 ± 0.039
6.162 ± 0.037
3.922 ± 0.025
7.071 ± 0.035
2.253 ± 0.025
5.548 ± 0.032
4.463 ± 0.038
10.934 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.489 ± 0.023
3.616 ± 0.029
4.265 ± 0.026
4.918 ± 0.038
5.009 ± 0.037
6.412 ± 0.033
5.112 ± 0.03
7.051 ± 0.045
1.362 ± 0.02
2.994 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here