Wenzhouxiangella sp. 15181
Average proteome isoelectric point is 5.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2733 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A372C136|A0A372C136_9GAMM Protein-L-isoaspartate O-methyltransferase OS=Wenzhouxiangella sp. 15181 OX=2301224 GN=pcm PE=3 SV=1
MM1 pKa = 8.08 DD2 pKa = 4.97 FNLMPLSLVISGLLVGNAAMAQNNLVIQPPAGGSVVVEE40 pKa = 4.15 DD41 pKa = 3.56 AAGNKK46 pKa = 9.14 VLLHH50 pKa = 6.4 IDD52 pKa = 3.64 EE53 pKa = 5.02 NGVLQLPGVAGSAQQATILCIDD75 pKa = 4.21 GASGQLGPCSAGTIAGLEE93 pKa = 4.34 GPIGPTGPTGARR105 pKa = 11.84 GPEE108 pKa = 4.21 GLQGPKK114 pKa = 10.31 GSPGDD119 pKa = 3.67 IGPTGPMGIQGDD131 pKa = 4.13 VGPTGAMGSPGPTGPMGPTGAAGVYY156 pKa = 9.6 TIGTGLEE163 pKa = 4.26 LNGDD167 pKa = 4.22 TLSLPTNCMDD177 pKa = 5.18 GAQLTWNATAASWDD191 pKa = 4.14 CPPVGPTGPTGPTGSTGG208 pKa = 3.06
Molecular weight: 19.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.719
IPC_protein 3.656
Toseland 3.452
ProMoST 3.846
Dawson 3.668
Bjellqvist 3.834
Wikipedia 3.63
Rodwell 3.49
Grimsley 3.363
Solomon 3.63
Lehninger 3.592
Nozaki 3.795
DTASelect 4.012
Thurlkill 3.528
EMBOSS 3.643
Sillero 3.783
Patrickios 0.693
IPC_peptide 3.63
IPC2_peptide 3.745
IPC2.peptide.svr19 3.714
Protein with the highest isoelectric point:
>tr|A0A372BU04|A0A372BU04_9GAMM Uncharacterized protein OS=Wenzhouxiangella sp. 15181 OX=2301224 GN=DZK25_11095 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.56 RR3 pKa = 11.84 TFQPSRR9 pKa = 11.84 IKK11 pKa = 10.39 RR12 pKa = 11.84 LRR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 5.73 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MASKK25 pKa = 10.28 SGRR28 pKa = 11.84 AIINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.63 GRR39 pKa = 11.84 KK40 pKa = 8.96 RR41 pKa = 11.84 LGANPGRR48 pKa = 4.09
Molecular weight: 5.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.568
Rodwell 12.735
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.457
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.237
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2733
0
2733
936914
22
2821
342.8
37.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.651 ± 0.05
0.845 ± 0.013
6.391 ± 0.043
7.276 ± 0.054
3.575 ± 0.029
8.386 ± 0.046
2.313 ± 0.024
4.887 ± 0.033
2.467 ± 0.04
10.671 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.32 ± 0.022
2.609 ± 0.024
4.919 ± 0.033
3.392 ± 0.026
7.851 ± 0.045
5.598 ± 0.039
4.748 ± 0.029
7.297 ± 0.035
1.586 ± 0.022
2.218 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here