Ilumatobacter fluminis
Average proteome isoelectric point is 5.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4258 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R7HXF4|A0A4R7HXF4_9ACTN NADH-quinone oxidoreductase subunit H OS=Ilumatobacter fluminis OX=467091 GN=nuoH PE=3 SV=1
MM1 pKa = 7.52 TEE3 pKa = 4.44 LANQMVMARR12 pKa = 11.84 DD13 pKa = 3.76 SDD15 pKa = 3.92 QLDD18 pKa = 5.02 LIDD21 pKa = 5.11 DD22 pKa = 5.18 CIDD25 pKa = 3.68 TVPAIYY31 pKa = 10.51 DD32 pKa = 3.52 GTAPNCWTTCPPAEE46 pKa = 4.15 RR47 pKa = 11.84 TFHH50 pKa = 7.09 LDD52 pKa = 2.9 VARR55 pKa = 11.84 NDD57 pKa = 3.45 QLTNIQDD64 pKa = 3.96 DD65 pKa = 4.25 VWWRR69 pKa = 11.84 TTLPVTYY76 pKa = 9.18 VTDD79 pKa = 4.12 DD80 pKa = 4.34 GIIDD84 pKa = 3.61 VTEE87 pKa = 3.88 YY88 pKa = 10.37 WSIRR92 pKa = 11.84 QVDD95 pKa = 3.63 DD96 pKa = 3.9 RR97 pKa = 11.84 FTVDD101 pKa = 2.95 EE102 pKa = 5.29 FEE104 pKa = 4.08 IQTPIMQRR112 pKa = 11.84 QSATDD117 pKa = 3.03 ILTVYY122 pKa = 9.78 FAHH125 pKa = 7.42 IDD127 pKa = 3.68 NEE129 pKa = 4.05 NWAQAADD136 pKa = 4.53 LLTQASSAPFGDD148 pKa = 4.76 RR149 pKa = 11.84 LDD151 pKa = 4.11 LQQLGANSYY160 pKa = 10.32 DD161 pKa = 3.37 QGDD164 pKa = 3.72 IADD167 pKa = 5.42 ALARR171 pKa = 11.84 WCADD175 pKa = 3.06 GCDD178 pKa = 3.79 TTPPSAAEE186 pKa = 3.79 LTFTGGYY193 pKa = 9.74 EE194 pKa = 4.03 LTRR197 pKa = 11.84 NAQTIRR203 pKa = 11.84 VTWYY207 pKa = 7.94 EE208 pKa = 3.96 GAYY211 pKa = 9.97 GIYY214 pKa = 10.25 GPPLRR219 pKa = 5.81
Molecular weight: 24.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.68
IPC2_protein 3.77
IPC_protein 3.795
Toseland 3.554
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.77
Rodwell 3.617
Grimsley 3.465
Solomon 3.795
Lehninger 3.757
Nozaki 3.91
DTASelect 4.202
Thurlkill 3.63
EMBOSS 3.77
Sillero 3.923
Patrickios 1.163
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.812
Protein with the highest isoelectric point:
>tr|A0A4R7I137|A0A4R7I137_9ACTN Lipoyl synthase OS=Ilumatobacter fluminis OX=467091 GN=lipA PE=3 SV=1
MM1 pKa = 7.79 RR2 pKa = 11.84 PRR4 pKa = 11.84 RR5 pKa = 11.84 SRR7 pKa = 11.84 SDD9 pKa = 3.02 PPTRR13 pKa = 11.84 SRR15 pKa = 11.84 GSRR18 pKa = 11.84 TPPALTPTRR27 pKa = 11.84 PHH29 pKa = 6.34 RR30 pKa = 11.84 RR31 pKa = 11.84 RR32 pKa = 11.84 PRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 HH40 pKa = 4.25 HH41 pKa = 5.95 RR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 RR45 pKa = 11.84 YY46 pKa = 9.94 DD47 pKa = 3.44 PPLHH51 pKa = 6.35 RR52 pKa = 11.84 AHH54 pKa = 6.7 GRR56 pKa = 11.84 RR57 pKa = 11.84 HH58 pKa = 3.6 VRR60 pKa = 11.84 RR61 pKa = 11.84 RR62 pKa = 11.84 TGHH65 pKa = 5.81 RR66 pKa = 11.84 RR67 pKa = 11.84 NHH69 pKa = 4.94 RR70 pKa = 11.84 HH71 pKa = 4.19 RR72 pKa = 11.84 HH73 pKa = 4.84 HH74 pKa = 6.84 RR75 pKa = 11.84 HH76 pKa = 4.81 HH77 pKa = 6.29 HH78 pKa = 4.41 HH79 pKa = 5.95 HH80 pKa = 5.86 RR81 pKa = 11.84 HH82 pKa = 4.86 RR83 pKa = 11.84 RR84 pKa = 11.84 SRR86 pKa = 11.84 PRR88 pKa = 11.84 QARR91 pKa = 11.84 CHH93 pKa = 5.95 RR94 pKa = 11.84 PLRR97 pKa = 11.84 RR98 pKa = 11.84 HH99 pKa = 5.7 RR100 pKa = 11.84 RR101 pKa = 11.84 RR102 pKa = 11.84 HH103 pKa = 4.79 QRR105 pKa = 11.84 DD106 pKa = 3.82 SVHH109 pKa = 6.42 LWPPTSPRR117 pKa = 11.84 FGRR120 pKa = 11.84 SICRR124 pKa = 11.84 RR125 pKa = 11.84 VACRR129 pKa = 11.84 SLRR132 pKa = 11.84 SRR134 pKa = 11.84 RR135 pKa = 11.84 SQNRR139 pKa = 11.84 RR140 pKa = 11.84 RR141 pKa = 11.84 RR142 pKa = 11.84 RR143 pKa = 11.84 VRR145 pKa = 11.84 HH146 pKa = 5.79 SSATSNDD153 pKa = 3.27 CPPRR157 pKa = 11.84 RR158 pKa = 11.84 RR159 pKa = 11.84 HH160 pKa = 4.85 RR161 pKa = 11.84 VRR163 pKa = 11.84 RR164 pKa = 11.84 RR165 pKa = 11.84 HH166 pKa = 4.05 RR167 pKa = 11.84 HH168 pKa = 4.42 RR169 pKa = 11.84 RR170 pKa = 11.84 LDD172 pKa = 3.28 RR173 pKa = 11.84 GRR175 pKa = 11.84 TVPPASRR182 pKa = 11.84 RR183 pKa = 11.84 QRR185 pKa = 11.84 RR186 pKa = 11.84 PVVPRR191 pKa = 11.84 PTPGRR196 pKa = 11.84 PGASSRR202 pKa = 11.84 RR203 pKa = 11.84 RR204 pKa = 11.84 RR205 pKa = 11.84 PLRR208 pKa = 11.84 DD209 pKa = 3.1 PARR212 pKa = 11.84 RR213 pKa = 11.84 PSGRR217 pKa = 11.84 PASSDD222 pKa = 2.81 GSAHH226 pKa = 6.56 HH227 pKa = 6.61 SPPGRR232 pKa = 11.84 RR233 pKa = 11.84 TWSIARR239 pKa = 11.84 PHH241 pKa = 5.4 PTPRR245 pKa = 11.84 RR246 pKa = 11.84 RR247 pKa = 11.84 RR248 pKa = 11.84 SPPPPARR255 pKa = 11.84 PRR257 pKa = 11.84 HH258 pKa = 4.77 RR259 pKa = 11.84 HH260 pKa = 4.73 FLSRR264 pKa = 11.84 PPGLRR269 pKa = 11.84 RR270 pKa = 11.84 RR271 pKa = 11.84 DD272 pKa = 3.49 RR273 pKa = 11.84 ASSRR277 pKa = 3.37
Molecular weight: 33.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.465
IPC2_protein 11.038
IPC_protein 12.632
Toseland 12.793
ProMoST 13.29
Dawson 12.793
Bjellqvist 12.793
Wikipedia 13.276
Rodwell 12.296
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.793
DTASelect 12.793
Thurlkill 12.793
EMBOSS 13.29
Sillero 12.793
Patrickios 12.003
IPC_peptide 13.29
IPC2_peptide 12.281
IPC2.peptide.svr19 9.164
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4258
0
4258
1475297
33
3915
346.5
37.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.18 ± 0.048
0.804 ± 0.011
7.852 ± 0.048
5.869 ± 0.037
3.142 ± 0.022
8.943 ± 0.036
2.141 ± 0.021
4.41 ± 0.028
1.605 ± 0.027
9.138 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.968 ± 0.022
2.095 ± 0.022
5.6 ± 0.029
2.546 ± 0.017
7.037 ± 0.054
5.467 ± 0.031
6.465 ± 0.055
9.248 ± 0.037
1.549 ± 0.018
1.94 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here