Streptomyces katsurahamanus
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 319 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A646J0F2|A0A646J0F2_9ACTN Uncharacterized protein (Fragment) OS=Streptomyces katsurahamanus OX=2577098 GN=FFZ77_24940 PE=4 SV=1
MM1 pKa = 7.46 YY2 pKa = 10.08 YY3 pKa = 10.49 VQVCDD8 pKa = 3.64 EE9 pKa = 4.36 TNSRR13 pKa = 11.84 DD14 pKa = 3.37 MGGFEE19 pKa = 5.16 LATTQSISSASLARR33 pKa = 11.84 YY34 pKa = 8.66 AVTAWDD40 pKa = 4.37 GFGLAYY46 pKa = 10.47 DD47 pKa = 4.68 FNADD51 pKa = 3.32 DD52 pKa = 4.93 LYY54 pKa = 11.35 SIRR57 pKa = 11.84 VWNGEE62 pKa = 4.15 DD63 pKa = 3.43 VVKK66 pKa = 10.78 LHH68 pKa = 6.66 TYY70 pKa = 10.6 RR71 pKa = 11.84 LIDD74 pKa = 3.59 GTLTAVV80 pKa = 3.22
Molecular weight: 8.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.934
IPC2_protein 4.215
IPC_protein 4.101
Toseland 3.897
ProMoST 4.279
Dawson 4.101
Bjellqvist 4.266
Wikipedia 4.062
Rodwell 3.935
Grimsley 3.808
Solomon 4.088
Lehninger 4.05
Nozaki 4.228
DTASelect 4.482
Thurlkill 3.961
EMBOSS 4.075
Sillero 4.228
Patrickios 0.846
IPC_peptide 4.088
IPC2_peptide 4.202
IPC2.peptide.svr19 4.109
Protein with the highest isoelectric point:
>tr|A0A646J3J4|A0A646J3J4_9ACTN ABC transporter permease (Fragment) OS=Streptomyces katsurahamanus OX=2577098 GN=FFZ77_21830 PE=4 SV=1
MM1 pKa = 6.63 PTKK4 pKa = 10.46 RR5 pKa = 11.84 GQAQRR10 pKa = 11.84 AEE12 pKa = 3.88 NEE14 pKa = 3.48 IRR16 pKa = 11.84 IRR18 pKa = 11.84 AAKK21 pKa = 10.15 DD22 pKa = 3.02 RR23 pKa = 11.84 LLCGDD28 pKa = 3.86 IPPRR32 pKa = 11.84 GKK34 pKa = 10.69 CDD36 pKa = 3.58 VKK38 pKa = 10.92 PLASEE43 pKa = 4.0 AAVDD47 pKa = 3.64 RR48 pKa = 11.84 TAFYY52 pKa = 8.29 GTRR55 pKa = 11.84 PYY57 pKa = 11.31 SDD59 pKa = 3.44 LRR61 pKa = 11.84 VEE63 pKa = 4.82 FGRR66 pKa = 11.84 RR67 pKa = 11.84 LNAMRR72 pKa = 11.84 EE73 pKa = 4.18 AGGIPDD79 pKa = 3.56 PRR81 pKa = 11.84 EE82 pKa = 3.86 AQVARR87 pKa = 11.84 LKK89 pKa = 10.94 AGNTRR94 pKa = 11.84 LRR96 pKa = 11.84 EE97 pKa = 3.65 RR98 pKa = 11.84 FAQSEE103 pKa = 4.21 RR104 pKa = 11.84 TINEE108 pKa = 4.35 LIDD111 pKa = 4.29 FRR113 pKa = 11.84 SQPLAQLAAQHH124 pKa = 5.67 EE125 pKa = 4.93 EE126 pKa = 3.72 IVRR129 pKa = 11.84 LRR131 pKa = 11.84 EE132 pKa = 3.84 AAAGTSTVSRR142 pKa = 11.84 LPAPRR147 pKa = 11.84 TTVIGTGSS155 pKa = 2.98
Molecular weight: 17.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.349
IPC2_protein 9.414
IPC_protein 10.379
Toseland 10.672
ProMoST 10.496
Dawson 10.745
Bjellqvist 10.511
Wikipedia 10.994
Rodwell 10.76
Grimsley 10.789
Solomon 10.95
Lehninger 10.906
Nozaki 10.672
DTASelect 10.496
Thurlkill 10.672
EMBOSS 11.096
Sillero 10.687
Patrickios 10.526
IPC_peptide 10.95
IPC2_peptide 9.692
IPC2.peptide.svr19 8.716
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
319
0
319
73503
21
910
230.4
24.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.723 ± 0.237
0.873 ± 0.039
5.832 ± 0.112
5.564 ± 0.128
2.641 ± 0.085
9.496 ± 0.15
2.428 ± 0.072
3.216 ± 0.103
1.69 ± 0.102
10.594 ± 0.188
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.701 ± 0.061
1.658 ± 0.08
6.171 ± 0.119
2.55 ± 0.087
8.762 ± 0.162
5.269 ± 0.108
5.947 ± 0.13
8.302 ± 0.145
1.497 ± 0.069
2.086 ± 0.084
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here