Streptomyces sp. GS7

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7398 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I6S3H6|A0A6I6S3H6_9ACTN DNA polymerase I OS=Streptomyces sp. GS7 OX=2692234 GN=polA PE=3 SV=1
MM1 pKa = 7.14TAQDD5 pKa = 3.89EE6 pKa = 4.47APEE9 pKa = 4.28LEE11 pKa = 4.17VWIDD15 pKa = 3.34QDD17 pKa = 3.99LCTGDD22 pKa = 5.01GICAQYY28 pKa = 10.9APEE31 pKa = 4.23VFEE34 pKa = 5.9LDD36 pKa = 3.05IDD38 pKa = 3.63GLAYY42 pKa = 10.42VKK44 pKa = 10.8SADD47 pKa = 5.02DD48 pKa = 3.79EE49 pKa = 4.41LLQEE53 pKa = 4.79KK54 pKa = 10.01GAATPVPLPLLNDD67 pKa = 3.32VRR69 pKa = 11.84DD70 pKa = 4.09SANEE74 pKa = 3.95CPGDD78 pKa = 4.29CIHH81 pKa = 6.28VRR83 pKa = 11.84RR84 pKa = 11.84VADD87 pKa = 3.62RR88 pKa = 11.84VEE90 pKa = 4.48VYY92 pKa = 10.88GPDD95 pKa = 3.33ADD97 pKa = 3.69

Molecular weight: 10.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I6SDI7|A0A6I6SDI7_9ACTN Aminotransferase class III-fold pyridoxal phosphate-dependent enzyme OS=Streptomyces sp. GS7 OX=2692234 GN=GR130_26695 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.36GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7398

0

7398

2422013

29

9496

327.4

34.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.121 ± 0.046

0.842 ± 0.008

5.832 ± 0.024

5.424 ± 0.028

2.684 ± 0.016

9.62 ± 0.028

2.469 ± 0.014

3.163 ± 0.021

2.016 ± 0.026

10.388 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.723 ± 0.012

1.694 ± 0.015

6.365 ± 0.032

2.841 ± 0.019

8.344 ± 0.036

4.829 ± 0.021

6.032 ± 0.026

8.133 ± 0.028

1.471 ± 0.012

2.01 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski