Amino acid dipepetide frequency for Streptomyces sp. GS7

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
23.508AlaAla: 23.508 ± 0.154
1.167AlaCys: 1.167 ± 0.022
8.613AlaAsp: 8.613 ± 0.071
8.696AlaGlu: 8.696 ± 0.102
3.578AlaPhe: 3.578 ± 0.042
14.06AlaGly: 14.06 ± 0.08
3.202AlaHis: 3.202 ± 0.043
3.557AlaIle: 3.557 ± 0.047
2.717AlaLys: 2.717 ± 0.054
15.124AlaLeu: 15.124 ± 0.11
2.596AlaMet: 2.596 ± 0.036
1.88AlaAsn: 1.88 ± 0.031
8.04AlaPro: 8.04 ± 0.097
3.947AlaGln: 3.947 ± 0.045
10.787AlaArg: 10.787 ± 0.072
5.588AlaSer: 5.588 ± 0.047
7.197AlaThr: 7.197 ± 0.06
12.491AlaVal: 12.491 ± 0.091
1.772AlaTrp: 1.772 ± 0.028
2.686AlaTyr: 2.686 ± 0.035
0.0AlaXaa: 0.0 ± 0.0
Cys
1.157CysAla: 1.157 ± 0.027
0.099CysCys: 0.099 ± 0.008
0.521CysAsp: 0.521 ± 0.014
0.386CysGlu: 0.386 ± 0.014
0.226CysPhe: 0.226 ± 0.01
1.035CysGly: 1.035 ± 0.021
0.215CysHis: 0.215 ± 0.008
0.197CysIle: 0.197 ± 0.009
0.118CysLys: 0.118 ± 0.008
0.807CysLeu: 0.807 ± 0.019
0.131CysMet: 0.131 ± 0.008
0.137CysAsn: 0.137 ± 0.008
0.526CysPro: 0.526 ± 0.015
0.184CysGln: 0.184 ± 0.009
0.712CysArg: 0.712 ± 0.018
0.452CysSer: 0.452 ± 0.016
0.546CysThr: 0.546 ± 0.015
0.649CysVal: 0.649 ± 0.016
0.143CysTrp: 0.143 ± 0.008
0.178CysTyr: 0.178 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
7.922AspAla: 7.922 ± 0.076
0.44AspCys: 0.44 ± 0.015
3.373AspAsp: 3.373 ± 0.042
3.55AspGlu: 3.55 ± 0.047
1.568AspPhe: 1.568 ± 0.027
6.514AspGly: 6.514 ± 0.063
1.489AspHis: 1.489 ± 0.028
1.856AspIle: 1.856 ± 0.029
1.064AspLys: 1.064 ± 0.026
5.987AspLeu: 5.987 ± 0.05
0.76AspMet: 0.76 ± 0.021
0.882AspAsn: 0.882 ± 0.02
4.507AspPro: 4.507 ± 0.046
1.531AspGln: 1.531 ± 0.027
5.153AspArg: 5.153 ± 0.051
2.293AspSer: 2.293 ± 0.033
3.097AspThr: 3.097 ± 0.034
4.282AspVal: 4.282 ± 0.045
1.005AspTrp: 1.005 ± 0.022
1.05AspTyr: 1.05 ± 0.021
0.0AspXaa: 0.0 ± 0.0
Glu
7.059GluAla: 7.059 ± 0.087
0.383GluCys: 0.383 ± 0.012
2.568GluAsp: 2.568 ± 0.037
3.392GluGlu: 3.392 ± 0.056
1.409GluPhe: 1.409 ± 0.029
4.009GluGly: 4.009 ± 0.045
1.506GluHis: 1.506 ± 0.027
2.205GluIle: 2.205 ± 0.033
1.271GluLys: 1.271 ± 0.03
6.711GluLeu: 6.711 ± 0.06
0.864GluMet: 0.864 ± 0.018
0.918GluAsn: 0.918 ± 0.022
3.271GluPro: 3.271 ± 0.048
2.182GluGln: 2.182 ± 0.031
5.559GluArg: 5.559 ± 0.056
2.326GluSer: 2.326 ± 0.034
2.71GluThr: 2.71 ± 0.035
4.213GluVal: 4.213 ± 0.047
0.704GluTrp: 0.704 ± 0.017
0.983GluTyr: 0.983 ± 0.021
0.0GluXaa: 0.0 ± 0.0
Phe
3.824PheAla: 3.824 ± 0.048
0.275PheCys: 0.275 ± 0.009
1.822PheAsp: 1.822 ± 0.033
1.262PheGlu: 1.262 ± 0.026
0.874PhePhe: 0.874 ± 0.021
3.014PheGly: 3.014 ± 0.037
0.671PheHis: 0.671 ± 0.016
0.698PheIle: 0.698 ± 0.017
0.456PheLys: 0.456 ± 0.016
2.623PheLeu: 2.623 ± 0.031
0.395PheMet: 0.395 ± 0.015
0.507PheAsn: 0.507 ± 0.016
1.418PhePro: 1.418 ± 0.025
0.658PheGln: 0.658 ± 0.017
1.851PheArg: 1.851 ± 0.029
1.445PheSer: 1.445 ± 0.029
1.98PheThr: 1.98 ± 0.029
2.099PheVal: 2.099 ± 0.028
0.412PheTrp: 0.412 ± 0.014
0.555PheTyr: 0.555 ± 0.017
0.0PheXaa: 0.0 ± 0.0
Gly
11.867GlyAla: 11.867 ± 0.092
0.914GlyCys: 0.914 ± 0.022
4.993GlyAsp: 4.993 ± 0.058
5.047GlyGlu: 5.047 ± 0.052
2.865GlyPhe: 2.865 ± 0.039
9.315GlyGly: 9.315 ± 0.09
2.493GlyHis: 2.493 ± 0.035
3.63GlyIle: 3.63 ± 0.041
2.343GlyLys: 2.343 ± 0.038
9.379GlyLeu: 9.379 ± 0.076
2.05GlyMet: 2.05 ± 0.03
1.775GlyAsn: 1.775 ± 0.03
5.611GlyPro: 5.611 ± 0.065
2.716GlyGln: 2.716 ± 0.041
8.277GlyArg: 8.277 ± 0.063
5.333GlySer: 5.333 ± 0.052
6.403GlyThr: 6.403 ± 0.059
7.265GlyVal: 7.265 ± 0.065
1.67GlyTrp: 1.67 ± 0.027
2.251GlyTyr: 2.251 ± 0.035
0.0GlyXaa: 0.0 ± 0.0
His
2.856HisAla: 2.856 ± 0.034
0.248HisCys: 0.248 ± 0.01
1.428HisAsp: 1.428 ± 0.026
1.196HisGlu: 1.196 ± 0.019
0.688HisPhe: 0.688 ± 0.015
2.662HisGly: 2.662 ± 0.035
0.824HisHis: 0.824 ± 0.022
0.722HisIle: 0.722 ± 0.019
0.372HisLys: 0.372 ± 0.011
2.607HisLeu: 2.607 ± 0.033
0.345HisMet: 0.345 ± 0.013
0.403HisAsn: 0.403 ± 0.014
2.053HisPro: 2.053 ± 0.039
0.742HisGln: 0.742 ± 0.018
2.38HisArg: 2.38 ± 0.034
1.116HisSer: 1.116 ± 0.025
1.534HisThr: 1.534 ± 0.031
1.624HisVal: 1.624 ± 0.029
0.39HisTrp: 0.39 ± 0.013
0.506HisTyr: 0.506 ± 0.015
0.0HisXaa: 0.0 ± 0.0
Ile
5.038IleAla: 5.038 ± 0.057
0.309IleCys: 0.309 ± 0.012
2.186IleAsp: 2.186 ± 0.032
1.941IleGlu: 1.941 ± 0.034
0.667IlePhe: 0.667 ± 0.018
3.626IleGly: 3.626 ± 0.04
0.642IleHis: 0.642 ± 0.019
0.829IleIle: 0.829 ± 0.021
0.718IleLys: 0.718 ± 0.018
2.289IleLeu: 2.289 ± 0.034
0.422IleMet: 0.422 ± 0.014
0.683IleAsn: 0.683 ± 0.018
1.816IlePro: 1.816 ± 0.028
0.716IleGln: 0.716 ± 0.017
2.423IleArg: 2.423 ± 0.034
1.65IleSer: 1.65 ± 0.032
2.171IleThr: 2.171 ± 0.033
2.628IleVal: 2.628 ± 0.043
0.355IleTrp: 0.355 ± 0.012
0.519IleTyr: 0.519 ± 0.015
0.0IleXaa: 0.0 ± 0.0
Lys
2.668LysAla: 2.668 ± 0.044
0.11LysCys: 0.11 ± 0.007
1.284LysAsp: 1.284 ± 0.028
1.102LysGlu: 1.102 ± 0.026
0.422LysPhe: 0.422 ± 0.015
1.77LysGly: 1.77 ± 0.038
0.425LysHis: 0.425 ± 0.014
0.83LysIle: 0.83 ± 0.023
0.796LysLys: 0.796 ± 0.028
1.864LysLeu: 1.864 ± 0.031
0.373LysMet: 0.373 ± 0.014
0.508LysAsn: 0.508 ± 0.016
1.285LysPro: 1.285 ± 0.027
0.652LysGln: 0.652 ± 0.02
1.377LysArg: 1.377 ± 0.025
1.083LysSer: 1.083 ± 0.023
1.2LysThr: 1.2 ± 0.026
1.758LysVal: 1.758 ± 0.03
0.244LysTrp: 0.244 ± 0.012
0.408LysTyr: 0.408 ± 0.015
0.0LysXaa: 0.0 ± 0.0
Leu
15.848LeuAla: 15.848 ± 0.119
0.9LeuCys: 0.9 ± 0.02
6.628LeuAsp: 6.628 ± 0.057
4.389LeuGlu: 4.389 ± 0.048
2.601LeuPhe: 2.601 ± 0.033
9.287LeuGly: 9.287 ± 0.074
2.552LeuHis: 2.552 ± 0.039
3.276LeuIle: 3.276 ± 0.052
1.851LeuLys: 1.851 ± 0.037
11.521LeuLeu: 11.521 ± 0.096
1.602LeuMet: 1.602 ± 0.026
1.617LeuAsn: 1.617 ± 0.028
6.772LeuPro: 6.772 ± 0.059
2.257LeuGln: 2.257 ± 0.033
9.064LeuArg: 9.064 ± 0.073
5.146LeuSer: 5.146 ± 0.049
6.918LeuThr: 6.918 ± 0.063
8.55LeuVal: 8.55 ± 0.07
1.315LeuTrp: 1.315 ± 0.029
1.784LeuTyr: 1.784 ± 0.023
0.0LeuXaa: 0.0 ± 0.0
Met
2.388MetAla: 2.388 ± 0.029
0.132MetCys: 0.132 ± 0.008
0.899MetAsp: 0.899 ± 0.021
0.737MetGlu: 0.737 ± 0.017
0.417MetPhe: 0.417 ± 0.013
1.354MetGly: 1.354 ± 0.028
0.377MetHis: 0.377 ± 0.011
0.602MetIle: 0.602 ± 0.016
0.408MetLys: 0.408 ± 0.012
1.672MetLeu: 1.672 ± 0.027
0.291MetMet: 0.291 ± 0.011
0.424MetAsn: 0.424 ± 0.014
1.168MetPro: 1.168 ± 0.027
0.441MetGln: 0.441 ± 0.015
1.428MetArg: 1.428 ± 0.024
1.207MetSer: 1.207 ± 0.023
1.488MetThr: 1.488 ± 0.026
1.285MetVal: 1.285 ± 0.023
0.199MetTrp: 0.199 ± 0.009
0.312MetTyr: 0.312 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
2.184AsnAla: 2.184 ± 0.035
0.169AsnCys: 0.169 ± 0.009
0.919AsnAsp: 0.919 ± 0.021
0.734AsnGlu: 0.734 ± 0.018
0.455AsnPhe: 0.455 ± 0.015
1.89AsnGly: 1.89 ± 0.037
0.424AsnHis: 0.424 ± 0.013
0.61AsnIle: 0.61 ± 0.017
0.392AsnLys: 0.392 ± 0.015
1.554AsnLeu: 1.554 ± 0.025
0.284AsnMet: 0.284 ± 0.013
0.401AsnAsn: 0.401 ± 0.013
1.32AsnPro: 1.32 ± 0.026
0.483AsnGln: 0.483 ± 0.016
1.249AsnArg: 1.249 ± 0.025
0.907AsnSer: 0.907 ± 0.02
1.038AsnThr: 1.038 ± 0.028
1.271AsnVal: 1.271 ± 0.025
0.274AsnTrp: 0.274 ± 0.012
0.386AsnTyr: 0.386 ± 0.015
0.0AsnXaa: 0.0 ± 0.0
Pro
9.555ProAla: 9.555 ± 0.105
0.377ProCys: 0.377 ± 0.014
4.656ProAsp: 4.656 ± 0.054
4.329ProGlu: 4.329 ± 0.05
1.571ProPhe: 1.571 ± 0.023
6.945ProGly: 6.945 ± 0.07
1.604ProHis: 1.604 ± 0.029
1.29ProIle: 1.29 ± 0.025
1.198ProLys: 1.198 ± 0.026
5.527ProLeu: 5.527 ± 0.054
1.011ProMet: 1.011 ± 0.018
0.9ProAsn: 0.9 ± 0.019
3.956ProPro: 3.956 ± 0.066
2.075ProGln: 2.075 ± 0.046
4.325ProArg: 4.325 ± 0.044
3.204ProSer: 3.204 ± 0.035
3.23ProThr: 3.23 ± 0.047
5.519ProVal: 5.519 ± 0.054
0.922ProTrp: 0.922 ± 0.02
1.453ProTyr: 1.453 ± 0.025
0.0ProXaa: 0.0 ± 0.0
Gln
3.841GlnAla: 3.841 ± 0.053
0.192GlnCys: 0.192 ± 0.01
1.431GlnAsp: 1.431 ± 0.024
1.466GlnGlu: 1.466 ± 0.029
0.667GlnPhe: 0.667 ± 0.017
2.34GlnGly: 2.34 ± 0.033
0.759GlnHis: 0.759 ± 0.024
1.074GlnIle: 1.074 ± 0.021
0.579GlnLys: 0.579 ± 0.019
3.346GlnLeu: 3.346 ± 0.037
0.51GlnMet: 0.51 ± 0.015
0.472GlnAsn: 0.472 ± 0.016
1.805GlnPro: 1.805 ± 0.043
1.412GlnGln: 1.412 ± 0.043
2.531GlnArg: 2.531 ± 0.039
1.243GlnSer: 1.243 ± 0.022
1.339GlnThr: 1.339 ± 0.024
2.338GlnVal: 2.338 ± 0.034
0.485GlnTrp: 0.485 ± 0.013
0.579GlnTyr: 0.579 ± 0.016
0.0GlnXaa: 0.0 ± 0.0
Arg
10.388ArgAla: 10.388 ± 0.08
0.672ArgCys: 0.672 ± 0.017
4.368ArgAsp: 4.368 ± 0.049
4.687ArgGlu: 4.687 ± 0.052
2.351ArgPhe: 2.351 ± 0.034
6.12ArgGly: 6.12 ± 0.053
2.3ArgHis: 2.3 ± 0.035
3.479ArgIle: 3.479 ± 0.038
1.642ArgLys: 1.642 ± 0.027
9.116ArgLeu: 9.116 ± 0.07
1.791ArgMet: 1.791 ± 0.028
1.403ArgAsn: 1.403 ± 0.028
5.492ArgPro: 5.492 ± 0.053
2.554ArgGln: 2.554 ± 0.037
8.41ArgArg: 8.41 ± 0.08
4.093ArgSer: 4.093 ± 0.037
5.757ArgThr: 5.757 ± 0.049
5.573ArgVal: 5.573 ± 0.051
1.382ArgTrp: 1.382 ± 0.025
1.861ArgTyr: 1.861 ± 0.029
0.0ArgXaa: 0.0 ± 0.0
Ser
6.7SerAla: 6.7 ± 0.05
0.42SerCys: 0.42 ± 0.012
2.416SerAsp: 2.416 ± 0.029
2.163SerGlu: 2.163 ± 0.034
1.449SerPhe: 1.449 ± 0.024
5.79SerGly: 5.79 ± 0.055
1.072SerHis: 1.072 ± 0.024
1.358SerIle: 1.358 ± 0.028
0.934SerLys: 0.934 ± 0.021
4.754SerLeu: 4.754 ± 0.048
0.994SerMet: 0.994 ± 0.021
0.794SerAsn: 0.794 ± 0.019
3.2SerPro: 3.2 ± 0.039
1.239SerGln: 1.239 ± 0.024
3.619SerArg: 3.619 ± 0.046
2.591SerSer: 2.591 ± 0.038
2.768SerThr: 2.768 ± 0.034
3.982SerVal: 3.982 ± 0.041
0.883SerTrp: 0.883 ± 0.022
1.158SerTyr: 1.158 ± 0.022
0.0SerXaa: 0.0 ± 0.0
Thr
9.442ThrAla: 9.442 ± 0.065
0.457ThrCys: 0.457 ± 0.013
3.481ThrAsp: 3.481 ± 0.041
3.023ThrGlu: 3.023 ± 0.034
1.584ThrPhe: 1.584 ± 0.021
6.768ThrGly: 6.768 ± 0.055
1.277ThrHis: 1.277 ± 0.025
1.63ThrIle: 1.63 ± 0.028
1.081ThrLys: 1.081 ± 0.028
5.638ThrLeu: 5.638 ± 0.053
0.918ThrMet: 0.918 ± 0.02
0.955ThrAsn: 0.955 ± 0.018
4.242ThrPro: 4.242 ± 0.049
1.302ThrGln: 1.302 ± 0.023
3.892ThrArg: 3.892 ± 0.045
2.945ThrSer: 2.945 ± 0.036
3.726ThrThr: 3.726 ± 0.044
5.887ThrVal: 5.887 ± 0.062
0.842ThrTrp: 0.842 ± 0.017
1.234ThrTyr: 1.234 ± 0.022
0.0ThrXaa: 0.0 ± 0.0
Val
10.544ValAla: 10.544 ± 0.074
0.801ValCys: 0.801 ± 0.018
4.619ValAsp: 4.619 ± 0.043
4.255ValGlu: 4.255 ± 0.043
2.321ValPhe: 2.321 ± 0.032
6.384ValGly: 6.384 ± 0.064
2.061ValHis: 2.061 ± 0.03
2.801ValIle: 2.801 ± 0.037
1.528ValLys: 1.528 ± 0.027
9.45ValLeu: 9.45 ± 0.074
1.363ValMet: 1.363 ± 0.026
1.578ValAsn: 1.578 ± 0.027
5.283ValPro: 5.283 ± 0.043
2.085ValGln: 2.085 ± 0.031
7.19ValArg: 7.19 ± 0.06
3.948ValSer: 3.948 ± 0.044
5.184ValThr: 5.184 ± 0.055
7.402ValVal: 7.402 ± 0.076
1.068ValTrp: 1.068 ± 0.02
1.462ValTyr: 1.462 ± 0.026
0.0ValXaa: 0.0 ± 0.0
Trp
1.642TrpAla: 1.642 ± 0.026
0.168TrpCys: 0.168 ± 0.009
0.777TrpAsp: 0.777 ± 0.018
0.7TrpGlu: 0.7 ± 0.019
0.497TrpPhe: 0.497 ± 0.014
1.041TrpGly: 1.041 ± 0.022
0.4TrpHis: 0.4 ± 0.012
0.524TrpIle: 0.524 ± 0.019
0.333TrpLys: 0.333 ± 0.012
1.795TrpLeu: 1.795 ± 0.032
0.298TrpMet: 0.298 ± 0.011
0.344TrpAsn: 0.344 ± 0.012
0.796TrpPro: 0.796 ± 0.021
0.634TrpGln: 0.634 ± 0.017
1.368TrpArg: 1.368 ± 0.029
0.867TrpSer: 0.867 ± 0.019
0.942TrpThr: 0.942 ± 0.018
0.938TrpVal: 0.938 ± 0.022
0.314TrpTrp: 0.314 ± 0.012
0.335TrpTyr: 0.335 ± 0.012
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.777TyrAla: 2.777 ± 0.036
0.186TyrCys: 0.186 ± 0.009
1.341TyrAsp: 1.341 ± 0.025
1.19TyrGlu: 1.19 ± 0.026
0.629TyrPhe: 0.629 ± 0.016
2.271TyrGly: 2.271 ± 0.03
0.402TyrHis: 0.402 ± 0.012
0.457TyrIle: 0.457 ± 0.015
0.357TyrLys: 0.357 ± 0.012
2.115TyrLeu: 2.115 ± 0.029
0.228TyrMet: 0.228 ± 0.011
0.362TyrAsn: 0.362 ± 0.014
1.086TyrPro: 1.086 ± 0.02
0.598TyrGln: 0.598 ± 0.016
1.844TyrArg: 1.844 ± 0.029
0.85TyrSer: 0.85 ± 0.018
1.098TyrThr: 1.098 ± 0.023
1.571TyrVal: 1.571 ± 0.027
0.335TyrTrp: 0.335 ± 0.011
0.407TyrTyr: 0.407 ± 0.012
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 7398 proteins (2422013 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski