Bacillus phage SPP1 (Bacteriophage SPP1)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 100 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|O48469|O48469_BPSPP Uncharacterized protein OS=Bacillus phage SPP1 OX=10724 PE=4 SV=1
MM1 pKa = 7.55SEE3 pKa = 4.13SLLYY7 pKa = 10.65GYY9 pKa = 10.64FLDD12 pKa = 3.75SWLDD16 pKa = 3.53GTASEE21 pKa = 4.22EE22 pKa = 4.09LLRR25 pKa = 11.84VAVNAGDD32 pKa = 3.91LTQEE36 pKa = 4.15EE37 pKa = 4.54ADD39 pKa = 4.75KK40 pKa = 11.23IMSYY44 pKa = 10.36PWGAWNDD51 pKa = 3.42

Molecular weight: 5.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|O48465|O48465_BPSPP Uncharacterized protein OS=Bacillus phage SPP1 OX=10724 PE=1 SV=1
MM1 pKa = 7.84LDD3 pKa = 3.33GLGGFLSGKK12 pKa = 9.5APSVTSVIPPPVFHH26 pKa = 6.93LPSFFLKK33 pKa = 10.15RR34 pKa = 11.84SLMAPICLSISSKK47 pKa = 10.54LRR49 pKa = 11.84YY50 pKa = 9.44SPNSIVAPSSGLSVKK65 pKa = 10.19YY66 pKa = 10.43VILMTSASNSIGVANSRR83 pKa = 11.84SRR85 pKa = 11.84IIQVSPSFTFLGATSTLL102 pKa = 3.7

Molecular weight: 10.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

97

3

100

16868

51

1111

168.7

19.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.978 ± 0.532

1.174 ± 0.202

5.371 ± 0.341

6.942 ± 0.501

4.903 ± 0.321

6.723 ± 0.308

1.672 ± 0.145

6.634 ± 0.236

7.571 ± 0.387

7.825 ± 0.29

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.929 ± 0.152

5.104 ± 0.324

4.079 ± 0.244

3.308 ± 0.206

4.95 ± 0.22

6.865 ± 0.435

5.721 ± 0.28

6.498 ± 0.236

1.209 ± 0.133

3.545 ± 0.267
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski