Amino acid dipepetide frequency for Bacillus phage SPP1 (Bacteriophage SPP1)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.411AlaAla: 7.411 ± 1.618
0.889AlaCys: 0.889 ± 0.282
3.557AlaAsp: 3.557 ± 0.511
4.862AlaGlu: 4.862 ± 0.811
3.498AlaPhe: 3.498 ± 0.578
4.743AlaGly: 4.743 ± 0.49
1.008AlaHis: 1.008 ± 0.252
4.387AlaIle: 4.387 ± 0.568
4.684AlaLys: 4.684 ± 0.713
5.929AlaLeu: 5.929 ± 0.679
1.482AlaMet: 1.482 ± 0.31
2.787AlaAsn: 2.787 ± 0.343
2.609AlaPro: 2.609 ± 0.511
2.49AlaGln: 2.49 ± 0.47
3.202AlaArg: 3.202 ± 0.556
4.862AlaSer: 4.862 ± 0.588
2.609AlaThr: 2.609 ± 0.42
5.158AlaVal: 5.158 ± 0.756
1.245AlaTrp: 1.245 ± 0.272
2.371AlaTyr: 2.371 ± 0.439
0.0AlaXaa: 0.0 ± 0.0
Cys
0.415CysAla: 0.415 ± 0.175
0.296CysCys: 0.296 ± 0.164
0.534CysAsp: 0.534 ± 0.205
0.534CysGlu: 0.534 ± 0.24
0.949CysPhe: 0.949 ± 0.258
1.008CysGly: 1.008 ± 0.316
0.356CysHis: 0.356 ± 0.13
0.711CysIle: 0.711 ± 0.231
0.711CysLys: 0.711 ± 0.246
0.593CysLeu: 0.593 ± 0.224
0.296CysMet: 0.296 ± 0.152
0.593CysAsn: 0.593 ± 0.215
0.711CysPro: 0.711 ± 0.254
0.237CysGln: 0.237 ± 0.124
0.771CysArg: 0.771 ± 0.282
1.423CysSer: 1.423 ± 0.453
0.415CysThr: 0.415 ± 0.194
0.889CysVal: 0.889 ± 0.326
0.119CysTrp: 0.119 ± 0.087
0.178CysTyr: 0.178 ± 0.098
0.0CysXaa: 0.0 ± 0.0
Asp
4.15AspAla: 4.15 ± 0.595
0.771AspCys: 0.771 ± 0.253
4.328AspAsp: 4.328 ± 0.715
3.735AspGlu: 3.735 ± 0.508
2.905AspPhe: 2.905 ± 0.42
4.328AspGly: 4.328 ± 0.463
0.83AspHis: 0.83 ± 0.211
4.091AspIle: 4.091 ± 0.54
3.735AspLys: 3.735 ± 0.651
4.269AspLeu: 4.269 ± 0.487
1.008AspMet: 1.008 ± 0.247
2.787AspAsn: 2.787 ± 0.576
2.016AspPro: 2.016 ± 0.335
0.711AspGln: 0.711 ± 0.218
2.431AspArg: 2.431 ± 0.391
3.32AspSer: 3.32 ± 0.493
3.083AspThr: 3.083 ± 0.531
2.609AspVal: 2.609 ± 0.403
0.534AspTrp: 0.534 ± 0.166
2.075AspTyr: 2.075 ± 0.292
0.0AspXaa: 0.0 ± 0.0
Glu
3.913GluAla: 3.913 ± 0.461
0.534GluCys: 0.534 ± 0.182
2.134GluAsp: 2.134 ± 0.325
6.759GluGlu: 6.759 ± 0.903
2.727GluPhe: 2.727 ± 0.375
4.387GluGly: 4.387 ± 0.419
1.186GluHis: 1.186 ± 0.234
4.684GluIle: 4.684 ± 0.561
5.039GluLys: 5.039 ± 0.721
6.699GluLeu: 6.699 ± 0.692
2.905GluMet: 2.905 ± 0.41
4.032GluAsn: 4.032 ± 0.48
2.846GluPro: 2.846 ± 0.432
3.379GluGln: 3.379 ± 0.548
3.735GluArg: 3.735 ± 0.484
3.735GluSer: 3.735 ± 0.481
2.964GluThr: 2.964 ± 0.405
4.15GluVal: 4.15 ± 0.508
1.008GluTrp: 1.008 ± 0.249
2.549GluTyr: 2.549 ± 0.412
0.0GluXaa: 0.0 ± 0.0
Phe
3.617PheAla: 3.617 ± 0.392
1.067PheCys: 1.067 ± 0.308
2.371PheAsp: 2.371 ± 0.352
2.787PheGlu: 2.787 ± 0.451
2.905PhePhe: 2.905 ± 0.487
3.142PheGly: 3.142 ± 0.407
1.008PheHis: 1.008 ± 0.281
3.142PheIle: 3.142 ± 0.479
3.32PheLys: 3.32 ± 0.394
4.269PheLeu: 4.269 ± 0.578
1.364PheMet: 1.364 ± 0.296
2.134PheAsn: 2.134 ± 0.316
2.134PhePro: 2.134 ± 0.395
2.016PheGln: 2.016 ± 0.377
1.304PheArg: 1.304 ± 0.249
3.024PheSer: 3.024 ± 0.441
3.439PheThr: 3.439 ± 0.461
3.261PheVal: 3.261 ± 0.586
0.889PheTrp: 0.889 ± 0.263
1.838PheTyr: 1.838 ± 0.373
0.0PheXaa: 0.0 ± 0.0
Gly
4.684GlyAla: 4.684 ± 0.634
0.711GlyCys: 0.711 ± 0.256
3.024GlyAsp: 3.024 ± 0.411
4.624GlyGlu: 4.624 ± 0.665
2.964GlyPhe: 2.964 ± 0.43
6.699GlyGly: 6.699 ± 0.696
1.126GlyHis: 1.126 ± 0.328
4.032GlyIle: 4.032 ± 0.523
5.039GlyLys: 5.039 ± 0.621
3.557GlyLeu: 3.557 ± 0.456
1.956GlyMet: 1.956 ± 0.318
2.905GlyAsn: 2.905 ± 0.471
2.016GlyPro: 2.016 ± 0.373
3.202GlyGln: 3.202 ± 0.454
3.498GlyArg: 3.498 ± 0.476
4.802GlySer: 4.802 ± 0.587
3.676GlyThr: 3.676 ± 0.45
4.684GlyVal: 4.684 ± 0.606
0.474GlyTrp: 0.474 ± 0.149
3.557GlyTyr: 3.557 ± 0.487
0.0GlyXaa: 0.0 ± 0.0
His
1.067HisAla: 1.067 ± 0.294
0.178HisCys: 0.178 ± 0.109
1.126HisAsp: 1.126 ± 0.283
1.008HisGlu: 1.008 ± 0.308
1.067HisPhe: 1.067 ± 0.279
1.067HisGly: 1.067 ± 0.231
0.296HisHis: 0.296 ± 0.138
0.889HisIle: 0.889 ± 0.225
1.067HisLys: 1.067 ± 0.205
1.364HisLeu: 1.364 ± 0.269
0.534HisMet: 0.534 ± 0.186
0.474HisAsn: 0.474 ± 0.156
0.711HisPro: 0.711 ± 0.197
0.237HisGln: 0.237 ± 0.117
1.186HisArg: 1.186 ± 0.292
1.245HisSer: 1.245 ± 0.23
0.771HisThr: 0.771 ± 0.226
1.364HisVal: 1.364 ± 0.314
0.415HisTrp: 0.415 ± 0.197
0.652HisTyr: 0.652 ± 0.195
0.0HisXaa: 0.0 ± 0.0
Ile
3.735IleAla: 3.735 ± 0.516
0.356IleCys: 0.356 ± 0.143
3.617IleAsp: 3.617 ± 0.448
4.862IleGlu: 4.862 ± 0.572
3.261IlePhe: 3.261 ± 0.569
3.735IleGly: 3.735 ± 0.54
1.008IleHis: 1.008 ± 0.279
4.684IleIle: 4.684 ± 0.622
6.107IleLys: 6.107 ± 0.598
5.039IleLeu: 5.039 ± 0.525
2.727IleMet: 2.727 ± 0.359
3.617IleAsn: 3.617 ± 0.542
2.549IlePro: 2.549 ± 0.421
2.549IleGln: 2.549 ± 0.448
2.253IleArg: 2.253 ± 0.324
4.565IleSer: 4.565 ± 0.68
4.447IleThr: 4.447 ± 0.69
4.743IleVal: 4.743 ± 0.562
0.593IleTrp: 0.593 ± 0.189
1.897IleTyr: 1.897 ± 0.386
0.0IleXaa: 0.0 ± 0.0
Lys
5.514LysAla: 5.514 ± 0.788
0.474LysCys: 0.474 ± 0.196
3.439LysAsp: 3.439 ± 0.535
5.751LysGlu: 5.751 ± 0.733
2.194LysPhe: 2.194 ± 0.392
4.802LysGly: 4.802 ± 0.551
1.541LysHis: 1.541 ± 0.308
4.091LysIle: 4.091 ± 0.586
7.945LysLys: 7.945 ± 0.943
5.929LysLeu: 5.929 ± 0.55
2.668LysMet: 2.668 ± 0.411
5.751LysAsn: 5.751 ± 0.648
2.49LysPro: 2.49 ± 0.482
2.371LysGln: 2.371 ± 0.434
4.921LysArg: 4.921 ± 0.536
4.269LysSer: 4.269 ± 0.634
4.387LysThr: 4.387 ± 0.576
4.032LysVal: 4.032 ± 0.465
1.008LysTrp: 1.008 ± 0.225
2.134LysTyr: 2.134 ± 0.434
0.0LysXaa: 0.0 ± 0.0
Leu
5.039LeuAla: 5.039 ± 0.553
1.067LeuCys: 1.067 ± 0.352
4.328LeuAsp: 4.328 ± 0.462
5.632LeuGlu: 5.632 ± 0.688
4.565LeuPhe: 4.565 ± 0.725
4.447LeuGly: 4.447 ± 0.592
1.186LeuHis: 1.186 ± 0.249
4.743LeuIle: 4.743 ± 0.61
6.462LeuLys: 6.462 ± 0.774
5.573LeuLeu: 5.573 ± 0.669
2.431LeuMet: 2.431 ± 0.384
3.735LeuAsn: 3.735 ± 0.569
3.202LeuPro: 3.202 ± 0.57
3.202LeuGln: 3.202 ± 0.509
4.091LeuArg: 4.091 ± 0.424
6.225LeuSer: 6.225 ± 0.737
5.573LeuThr: 5.573 ± 0.583
3.557LeuVal: 3.557 ± 0.515
0.593LeuTrp: 0.593 ± 0.181
2.609LeuTyr: 2.609 ± 0.433
0.0LeuXaa: 0.0 ± 0.0
Met
2.846MetAla: 2.846 ± 0.593
0.237MetCys: 0.237 ± 0.122
1.126MetAsp: 1.126 ± 0.268
1.126MetGlu: 1.126 ± 0.267
1.186MetPhe: 1.186 ± 0.269
1.423MetGly: 1.423 ± 0.311
0.415MetHis: 0.415 ± 0.171
2.194MetIle: 2.194 ± 0.393
2.431MetLys: 2.431 ± 0.425
1.364MetLeu: 1.364 ± 0.266
0.83MetMet: 0.83 ± 0.252
2.312MetAsn: 2.312 ± 0.294
1.541MetPro: 1.541 ± 0.28
1.186MetGln: 1.186 ± 0.28
1.66MetArg: 1.66 ± 0.301
2.964MetSer: 2.964 ± 0.468
1.779MetThr: 1.779 ± 0.293
1.245MetVal: 1.245 ± 0.28
0.356MetTrp: 0.356 ± 0.158
1.067MetTyr: 1.067 ± 0.228
0.0MetXaa: 0.0 ± 0.0
Asn
4.091AsnAla: 4.091 ± 0.678
0.771AsnCys: 0.771 ± 0.254
3.972AsnAsp: 3.972 ± 0.518
4.091AsnGlu: 4.091 ± 0.539
2.075AsnPhe: 2.075 ± 0.365
4.506AsnGly: 4.506 ± 0.548
0.771AsnHis: 0.771 ± 0.237
3.676AsnIle: 3.676 ± 0.587
3.202AsnLys: 3.202 ± 0.42
3.617AsnLeu: 3.617 ± 0.36
1.601AsnMet: 1.601 ± 0.255
2.253AsnAsn: 2.253 ± 0.449
2.668AsnPro: 2.668 ± 0.48
1.008AsnGln: 1.008 ± 0.24
2.312AsnArg: 2.312 ± 0.316
2.787AsnSer: 2.787 ± 0.424
2.668AsnThr: 2.668 ± 0.393
3.083AsnVal: 3.083 ± 0.641
0.296AsnTrp: 0.296 ± 0.12
1.601AsnTyr: 1.601 ± 0.323
0.0AsnXaa: 0.0 ± 0.0
Pro
2.253ProAla: 2.253 ± 0.355
0.889ProCys: 0.889 ± 0.293
1.897ProAsp: 1.897 ± 0.393
2.905ProGlu: 2.905 ± 0.482
2.549ProPhe: 2.549 ± 0.497
2.431ProGly: 2.431 ± 0.393
0.949ProHis: 0.949 ± 0.221
2.668ProIle: 2.668 ± 0.364
2.668ProLys: 2.668 ± 0.347
4.091ProLeu: 4.091 ± 0.5
0.83ProMet: 0.83 ± 0.182
1.541ProAsn: 1.541 ± 0.317
2.253ProPro: 2.253 ± 0.451
1.186ProGln: 1.186 ± 0.208
1.186ProArg: 1.186 ± 0.303
3.794ProSer: 3.794 ± 0.672
1.482ProThr: 1.482 ± 0.351
3.617ProVal: 3.617 ± 0.501
0.415ProTrp: 0.415 ± 0.162
1.186ProTyr: 1.186 ± 0.248
0.0ProXaa: 0.0 ± 0.0
Gln
2.549GlnAla: 2.549 ± 0.508
0.059GlnCys: 0.059 ± 0.058
2.016GlnAsp: 2.016 ± 0.345
2.194GlnGlu: 2.194 ± 0.415
1.364GlnPhe: 1.364 ± 0.364
1.66GlnGly: 1.66 ± 0.301
0.415GlnHis: 0.415 ± 0.164
1.779GlnIle: 1.779 ± 0.285
2.846GlnLys: 2.846 ± 0.48
3.261GlnLeu: 3.261 ± 0.49
0.949GlnMet: 0.949 ± 0.353
1.719GlnAsn: 1.719 ± 0.33
1.245GlnPro: 1.245 ± 0.241
1.541GlnGln: 1.541 ± 0.362
1.719GlnArg: 1.719 ± 0.343
2.016GlnSer: 2.016 ± 0.325
2.134GlnThr: 2.134 ± 0.361
2.371GlnVal: 2.371 ± 0.372
0.237GlnTrp: 0.237 ± 0.105
1.008GlnTyr: 1.008 ± 0.211
0.0GlnXaa: 0.0 ± 0.0
Arg
2.609ArgAla: 2.609 ± 0.379
0.593ArgCys: 0.593 ± 0.164
2.253ArgAsp: 2.253 ± 0.363
3.32ArgGlu: 3.32 ± 0.451
2.727ArgPhe: 2.727 ± 0.479
3.261ArgGly: 3.261 ± 0.406
1.245ArgHis: 1.245 ± 0.21
4.209ArgIle: 4.209 ± 0.612
3.794ArgLys: 3.794 ± 0.516
4.447ArgLeu: 4.447 ± 0.572
1.719ArgMet: 1.719 ± 0.332
1.956ArgAsn: 1.956 ± 0.281
1.66ArgPro: 1.66 ± 0.33
1.186ArgGln: 1.186 ± 0.283
3.202ArgArg: 3.202 ± 0.483
1.719ArgSer: 1.719 ± 0.355
3.32ArgThr: 3.32 ± 0.465
3.557ArgVal: 3.557 ± 0.461
0.534ArgTrp: 0.534 ± 0.206
2.194ArgTyr: 2.194 ± 0.376
0.0ArgXaa: 0.0 ± 0.0
Ser
6.107SerAla: 6.107 ± 0.666
1.186SerCys: 1.186 ± 0.386
3.439SerAsp: 3.439 ± 0.361
3.142SerGlu: 3.142 ± 0.38
4.091SerPhe: 4.091 ± 0.598
5.573SerGly: 5.573 ± 0.605
0.949SerHis: 0.949 ± 0.221
4.447SerIle: 4.447 ± 0.539
3.854SerLys: 3.854 ± 0.483
5.751SerLeu: 5.751 ± 0.838
1.779SerMet: 1.779 ± 0.284
2.549SerAsn: 2.549 ± 0.436
2.016SerPro: 2.016 ± 0.37
1.541SerGln: 1.541 ± 0.29
3.913SerArg: 3.913 ± 0.548
5.869SerSer: 5.869 ± 0.997
3.913SerThr: 3.913 ± 0.434
5.336SerVal: 5.336 ± 0.628
1.008SerTrp: 1.008 ± 0.24
2.194SerTyr: 2.194 ± 0.316
0.0SerXaa: 0.0 ± 0.0
Thr
4.269ThrAla: 4.269 ± 0.661
0.534ThrCys: 0.534 ± 0.235
3.261ThrAsp: 3.261 ± 0.566
3.972ThrGlu: 3.972 ± 0.538
2.964ThrPhe: 2.964 ± 0.363
4.387ThrGly: 4.387 ± 0.586
0.652ThrHis: 0.652 ± 0.225
3.913ThrIle: 3.913 ± 0.535
3.617ThrLys: 3.617 ± 0.469
3.972ThrLeu: 3.972 ± 0.519
1.423ThrMet: 1.423 ± 0.31
2.312ThrAsn: 2.312 ± 0.444
3.261ThrPro: 3.261 ± 0.476
1.423ThrGln: 1.423 ± 0.3
2.49ThrArg: 2.49 ± 0.406
4.15ThrSer: 4.15 ± 0.609
3.024ThrThr: 3.024 ± 0.507
4.921ThrVal: 4.921 ± 0.621
0.534ThrTrp: 0.534 ± 0.179
2.134ThrTyr: 2.134 ± 0.308
0.0ThrXaa: 0.0 ± 0.0
Val
3.142ValAla: 3.142 ± 0.418
0.534ValCys: 0.534 ± 0.199
3.854ValAsp: 3.854 ± 0.49
4.565ValGlu: 4.565 ± 0.58
3.142ValPhe: 3.142 ± 0.663
2.253ValGly: 2.253 ± 0.354
1.008ValHis: 1.008 ± 0.237
4.684ValIle: 4.684 ± 0.384
6.107ValLys: 6.107 ± 0.65
4.743ValLeu: 4.743 ± 0.616
1.601ValMet: 1.601 ± 0.337
4.328ValAsn: 4.328 ± 0.474
3.024ValPro: 3.024 ± 0.441
1.838ValGln: 1.838 ± 0.354
2.964ValArg: 2.964 ± 0.394
4.802ValSer: 4.802 ± 0.591
4.98ValThr: 4.98 ± 0.687
3.439ValVal: 3.439 ± 0.506
1.482ValTrp: 1.482 ± 0.489
2.49ValTyr: 2.49 ± 0.431
0.0ValXaa: 0.0 ± 0.0
Trp
0.474TrpAla: 0.474 ± 0.159
0.0TrpCys: 0.0 ± 0.0
0.711TrpAsp: 0.711 ± 0.226
0.356TrpGlu: 0.356 ± 0.138
0.296TrpPhe: 0.296 ± 0.187
1.067TrpGly: 1.067 ± 0.188
0.356TrpHis: 0.356 ± 0.154
1.008TrpIle: 1.008 ± 0.236
0.771TrpLys: 0.771 ± 0.226
0.474TrpLeu: 0.474 ± 0.187
0.237TrpMet: 0.237 ± 0.124
1.304TrpAsn: 1.304 ± 0.52
0.593TrpPro: 0.593 ± 0.257
0.652TrpGln: 0.652 ± 0.181
0.711TrpArg: 0.711 ± 0.211
0.889TrpSer: 0.889 ± 0.206
0.771TrpThr: 0.771 ± 0.203
1.008TrpVal: 1.008 ± 0.203
0.059TrpTrp: 0.059 ± 0.067
0.356TrpTyr: 0.356 ± 0.147
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.897TyrAla: 1.897 ± 0.35
0.593TyrCys: 0.593 ± 0.156
2.727TyrAsp: 2.727 ± 0.392
3.202TyrGlu: 3.202 ± 0.602
1.601TyrPhe: 1.601 ± 0.252
2.312TyrGly: 2.312 ± 0.329
0.415TyrHis: 0.415 ± 0.141
2.312TyrIle: 2.312 ± 0.428
2.312TyrLys: 2.312 ± 0.418
3.32TyrLeu: 3.32 ± 0.382
0.889TyrMet: 0.889 ± 0.221
1.956TyrAsn: 1.956 ± 0.365
1.245TyrPro: 1.245 ± 0.325
1.126TyrGln: 1.126 ± 0.328
1.956TyrArg: 1.956 ± 0.393
2.194TyrSer: 2.194 ± 0.401
1.779TyrThr: 1.779 ± 0.274
1.956TyrVal: 1.956 ± 0.325
0.296TyrTrp: 0.296 ± 0.135
1.364TyrTyr: 1.364 ± 0.351
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 100 proteins (16868 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski