Lactobacillus salivarius (strain UCC118)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Ligilactobacillus; Ligilactobacillus salivarius

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1998 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q1WRD3|Q1WRD3_LACS1 Uncharacterized protein OS=Lactobacillus salivarius (strain UCC118) OX=362948 GN=LSL_1760 PE=4 SV=1
MM1 pKa = 7.75EE2 pKa = 6.46DD3 pKa = 2.46IGYY6 pKa = 10.21LYY8 pKa = 10.57FSQDD12 pKa = 3.02DD13 pKa = 3.91YY14 pKa = 12.21NFIKK18 pKa = 10.51EE19 pKa = 4.14HH20 pKa = 6.4FPDD23 pKa = 3.5IFAICEE29 pKa = 3.98QYY31 pKa = 10.98VSSRR35 pKa = 11.84EE36 pKa = 3.67PDD38 pKa = 3.06IEE40 pKa = 4.27LTVTDD45 pKa = 4.67SQTDD49 pKa = 3.75MIDD52 pKa = 3.31NKK54 pKa = 10.43VLMAIGSSATAPYY67 pKa = 10.37GNPSPEE73 pKa = 4.75AIEE76 pKa = 5.95LEE78 pKa = 4.66AIWDD82 pKa = 3.88RR83 pKa = 11.84AA84 pKa = 3.44

Molecular weight: 9.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q1WRG2|Q1WRG2_LACS1 Hypothetical membrane spanning protein OS=Lactobacillus salivarius (strain UCC118) OX=362948 GN=LSL_1734 PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.69QPKK8 pKa = 8.69KK9 pKa = 7.77RR10 pKa = 11.84HH11 pKa = 4.99RR12 pKa = 11.84QRR14 pKa = 11.84VHH16 pKa = 6.07GFRR19 pKa = 11.84KK20 pKa = 10.03RR21 pKa = 11.84MSTSNGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1998

0

1998

580046

35

1444

290.3

32.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.664 ± 0.066

0.558 ± 0.015

5.875 ± 0.051

6.626 ± 0.069

4.31 ± 0.045

6.311 ± 0.05

1.791 ± 0.024

7.909 ± 0.062

8.085 ± 0.069

9.617 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.623 ± 0.027

5.593 ± 0.049

3.151 ± 0.032

3.641 ± 0.042

3.82 ± 0.037

6.061 ± 0.051

5.536 ± 0.038

6.896 ± 0.049

0.963 ± 0.02

3.97 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski