Amino acid dipepetide frequency for Lactobacillus salivarius (strain UCC118)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.06AlaAla: 5.06 ± 0.128	0.431AlaCys: 0.431 ± 0.028	3.845AlaAsp: 3.845 ± 0.084	4.277AlaGlu: 4.277 ± 0.092	2.688AlaPhe: 2.688 ± 0.076	4.634AlaGly: 4.634 ± 0.101	1.14AlaHis: 1.14 ± 0.04	5.494AlaIle: 5.494 ± 0.113	5.594AlaLys: 5.594 ± 0.108	6.406AlaLeu: 6.406 ± 0.119	1.962AlaMet: 1.962 ± 0.059	3.315AlaAsn: 3.315 ± 0.081	1.76AlaPro: 1.76 ± 0.058	2.541AlaGln: 2.541 ± 0.089	2.538AlaArg: 2.538 ± 0.076	3.581AlaSer: 3.581 ± 0.094	3.926AlaThr: 3.926 ± 0.084	4.494AlaVal: 4.494 ± 0.099	0.595AlaTrp: 0.595 ± 0.029	2.36AlaTyr: 2.36 ± 0.071	0.0AlaXaa: 0.0 ± 0.0
Cys	0.319CysAla: 0.319 ± 0.023	0.052CysCys: 0.052 ± 0.01	0.307CysAsp: 0.307 ± 0.02	0.341CysGlu: 0.341 ± 0.027	0.222CysPhe: 0.222 ± 0.018	0.478CysGly: 0.478 ± 0.032	0.138CysHis: 0.138 ± 0.017	0.443CysIle: 0.443 ± 0.031	0.328CysLys: 0.328 ± 0.026	0.578CysLeu: 0.578 ± 0.033	0.112CysMet: 0.112 ± 0.014	0.24CysAsn: 0.24 ± 0.02	0.243CysPro: 0.243 ± 0.022	0.219CysGln: 0.219 ± 0.018	0.234CysArg: 0.234 ± 0.021	0.4CysSer: 0.4 ± 0.03	0.29CysThr: 0.29 ± 0.024	0.321CysVal: 0.321 ± 0.023	0.069CysTrp: 0.069 ± 0.012	0.247CysTyr: 0.247 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	3.614AspAla: 3.614 ± 0.079	0.269AspCys: 0.269 ± 0.024	3.743AspAsp: 3.743 ± 0.094	4.796AspGlu: 4.796 ± 0.102	2.857AspPhe: 2.857 ± 0.077	3.681AspGly: 3.681 ± 0.097	0.824AspHis: 0.824 ± 0.038	4.581AspIle: 4.581 ± 0.097	5.082AspLys: 5.082 ± 0.119	5.229AspLeu: 5.229 ± 0.101	1.574AspMet: 1.574 ± 0.059	3.426AspAsn: 3.426 ± 0.089	1.667AspPro: 1.667 ± 0.052	1.545AspGln: 1.545 ± 0.054	2.055AspArg: 2.055 ± 0.069	3.248AspSer: 3.248 ± 0.077	3.07AspThr: 3.07 ± 0.079	4.067AspVal: 4.067 ± 0.098	0.648AspTrp: 0.648 ± 0.035	2.774AspTyr: 2.774 ± 0.065	0.0AspXaa: 0.0 ± 0.0
Glu	4.208GluAla: 4.208 ± 0.108	0.284GluCys: 0.284 ± 0.028	3.776GluAsp: 3.776 ± 0.092	5.079GluGlu: 5.079 ± 0.112	2.586GluPhe: 2.586 ± 0.077	3.133GluGly: 3.133 ± 0.085	1.14GluHis: 1.14 ± 0.044	5.256GluIle: 5.256 ± 0.089	6.539GluLys: 6.539 ± 0.133	7.017GluLeu: 7.017 ± 0.132	1.924GluMet: 1.924 ± 0.065	4.22GluAsn: 4.22 ± 0.094	1.598GluPro: 1.598 ± 0.047	2.558GluGln: 2.558 ± 0.079	2.752GluArg: 2.752 ± 0.076	3.343GluSer: 3.343 ± 0.08	3.074GluThr: 3.074 ± 0.075	4.56GluVal: 4.56 ± 0.112	0.638GluTrp: 0.638 ± 0.033	2.57GluTyr: 2.57 ± 0.069	0.0GluXaa: 0.0 ± 0.0
Phe	2.67PheAla: 2.67 ± 0.08	0.297PheCys: 0.297 ± 0.027	2.827PheAsp: 2.827 ± 0.081	2.643PheGlu: 2.643 ± 0.067	2.088PhePhe: 2.088 ± 0.069	2.964PheGly: 2.964 ± 0.08	0.7PheHis: 0.7 ± 0.038	3.619PheIle: 3.619 ± 0.102	3.234PheLys: 3.234 ± 0.068	3.976PheLeu: 3.976 ± 0.108	1.141PheMet: 1.141 ± 0.049	2.453PheAsn: 2.453 ± 0.07	1.374PhePro: 1.374 ± 0.046	1.195PheGln: 1.195 ± 0.048	1.353PheArg: 1.353 ± 0.049	2.903PheSer: 2.903 ± 0.067	2.426PheThr: 2.426 ± 0.065	2.996PheVal: 2.996 ± 0.078	0.519PheTrp: 0.519 ± 0.032	1.724PheTyr: 1.724 ± 0.057	0.0PheXaa: 0.0 ± 0.0
Gly	4.241GlyAla: 4.241 ± 0.107	0.345GlyCys: 0.345 ± 0.023	3.224GlyAsp: 3.224 ± 0.085	3.51GlyGlu: 3.51 ± 0.08	2.814GlyPhe: 2.814 ± 0.073	3.936GlyGly: 3.936 ± 0.111	1.224GlyHis: 1.224 ± 0.044	5.937GlyIle: 5.937 ± 0.126	5.256GlyLys: 5.256 ± 0.109	5.991GlyLeu: 5.991 ± 0.113	1.907GlyMet: 1.907 ± 0.066	3.126GlyAsn: 3.126 ± 0.092	1.334GlyPro: 1.334 ± 0.049	2.243GlyGln: 2.243 ± 0.079	2.426GlyArg: 2.426 ± 0.065	3.696GlySer: 3.696 ± 0.086	3.748GlyThr: 3.748 ± 0.077	4.596GlyVal: 4.596 ± 0.099	0.678GlyTrp: 0.678 ± 0.038	2.883GlyTyr: 2.883 ± 0.067	0.0GlyXaa: 0.0 ± 0.0
His	1.091HisAla: 1.091 ± 0.041	0.102HisCys: 0.102 ± 0.017	1.095HisAsp: 1.095 ± 0.042	1.072HisGlu: 1.072 ± 0.039	0.879HisPhe: 0.879 ± 0.039	1.319HisGly: 1.319 ± 0.055	0.538HisHis: 0.538 ± 0.036	1.222HisIle: 1.222 ± 0.048	1.169HisLys: 1.169 ± 0.041	1.645HisLeu: 1.645 ± 0.056	0.428HisMet: 0.428 ± 0.026	0.915HisAsn: 0.915 ± 0.04	0.857HisPro: 0.857 ± 0.038	0.786HisGln: 0.786 ± 0.036	0.753HisArg: 0.753 ± 0.041	0.979HisSer: 0.979 ± 0.043	0.884HisThr: 0.884 ± 0.041	1.188HisVal: 1.188 ± 0.046	0.2HisTrp: 0.2 ± 0.023	0.791HisTyr: 0.791 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	5.629IleAla: 5.629 ± 0.105	0.576IleCys: 0.576 ± 0.032	4.898IleAsp: 4.898 ± 0.107	4.681IleGlu: 4.681 ± 0.098	3.705IlePhe: 3.705 ± 0.105	5.2IleGly: 5.2 ± 0.109	1.241IleHis: 1.241 ± 0.049	6.377IleIle: 6.377 ± 0.158	5.843IleLys: 5.843 ± 0.107	7.458IleLeu: 7.458 ± 0.141	1.857IleMet: 1.857 ± 0.054	4.362IleAsn: 4.362 ± 0.084	2.915IlePro: 2.915 ± 0.082	2.377IleGln: 2.377 ± 0.064	2.891IleArg: 2.891 ± 0.074	5.622IleSer: 5.622 ± 0.106	4.529IleThr: 4.529 ± 0.09	5.486IleVal: 5.486 ± 0.122	0.624IleTrp: 0.624 ± 0.036	2.815IleTyr: 2.815 ± 0.076	0.0IleXaa: 0.0 ± 0.0
Lys	5.055LysAla: 5.055 ± 0.122	0.281LysCys: 0.281 ± 0.025	4.767LysAsp: 4.767 ± 0.099	6.305LysGlu: 6.305 ± 0.119	2.984LysPhe: 2.984 ± 0.077	3.879LysGly: 3.879 ± 0.094	1.419LysHis: 1.419 ± 0.052	6.47LysIle: 6.47 ± 0.127	7.606LysLys: 7.606 ± 0.148	7.605LysLeu: 7.605 ± 0.127	2.526LysMet: 2.526 ± 0.059	5.184LysAsn: 5.184 ± 0.111	1.936LysPro: 1.936 ± 0.057	3.348LysGln: 3.348 ± 0.092	3.369LysArg: 3.369 ± 0.083	4.003LysSer: 4.003 ± 0.09	4.029LysThr: 4.029 ± 0.102	5.829LysVal: 5.829 ± 0.114	0.662LysTrp: 0.662 ± 0.033	3.591LysTyr: 3.591 ± 0.079	0.0LysXaa: 0.0 ± 0.0
Leu	7.198LeuAla: 7.198 ± 0.135	0.591LeuCys: 0.591 ± 0.034	5.775LeuAsp: 5.775 ± 0.104	6.101LeuGlu: 6.101 ± 0.122	3.979LeuPhe: 3.979 ± 0.095	6.384LeuGly: 6.384 ± 0.121	1.705LeuHis: 1.705 ± 0.048	7.224LeuIle: 7.224 ± 0.15	7.343LeuLys: 7.343 ± 0.123	8.932LeuLeu: 8.932 ± 0.16	2.326LeuMet: 2.326 ± 0.069	5.298LeuAsn: 5.298 ± 0.102	3.584LeuPro: 3.584 ± 0.073	3.357LeuGln: 3.357 ± 0.091	3.646LeuArg: 3.646 ± 0.092	6.624LeuSer: 6.624 ± 0.116	5.753LeuThr: 5.753 ± 0.094	6.563LeuVal: 6.563 ± 0.11	0.821LeuTrp: 0.821 ± 0.038	2.969LeuTyr: 2.969 ± 0.088	0.0LeuXaa: 0.0 ± 0.0
Met	1.905MetAla: 1.905 ± 0.061	0.157MetCys: 0.157 ± 0.019	1.464MetAsp: 1.464 ± 0.053	1.572MetGlu: 1.572 ± 0.05	1.026MetPhe: 1.026 ± 0.043	1.658MetGly: 1.658 ± 0.057	0.434MetHis: 0.434 ± 0.028	2.089MetIle: 2.089 ± 0.062	2.264MetLys: 2.264 ± 0.067	2.46MetLeu: 2.46 ± 0.069	0.74MetMet: 0.74 ± 0.036	1.512MetAsn: 1.512 ± 0.051	0.981MetPro: 0.981 ± 0.042	0.988MetGln: 0.988 ± 0.043	1.019MetArg: 1.019 ± 0.04	1.677MetSer: 1.677 ± 0.061	1.619MetThr: 1.619 ± 0.059	1.676MetVal: 1.676 ± 0.049	0.178MetTrp: 0.178 ± 0.016	0.809MetTyr: 0.809 ± 0.036	0.0MetXaa: 0.0 ± 0.0
Asn	3.21AsnAla: 3.21 ± 0.088	0.309AsnCys: 0.309 ± 0.023	3.157AsnAsp: 3.157 ± 0.081	3.624AsnGlu: 3.624 ± 0.09	2.512AsnPhe: 2.512 ± 0.072	3.712AsnGly: 3.712 ± 0.086	1.103AsnHis: 1.103 ± 0.045	4.229AsnIle: 4.229 ± 0.104	4.386AsnLys: 4.386 ± 0.094	5.45AsnLeu: 5.45 ± 0.113	1.421AsnMet: 1.421 ± 0.047	3.641AsnAsn: 3.641 ± 0.117	2.179AsnPro: 2.179 ± 0.061	2.203AsnGln: 2.203 ± 0.072	2.1AsnArg: 2.1 ± 0.061	3.301AsnSer: 3.301 ± 0.086	2.776AsnThr: 2.776 ± 0.082	3.46AsnVal: 3.46 ± 0.089	0.712AsnTrp: 0.712 ± 0.037	2.448AsnTyr: 2.448 ± 0.071	0.0AsnXaa: 0.0 ± 0.0
Pro	2.072ProAla: 2.072 ± 0.066	0.143ProCys: 0.143 ± 0.014	2.067ProAsp: 2.067 ± 0.06	2.715ProGlu: 2.715 ± 0.06	1.509ProPhe: 1.509 ± 0.057	1.884ProGly: 1.884 ± 0.055	0.607ProHis: 0.607 ± 0.032	2.358ProIle: 2.358 ± 0.066	2.326ProLys: 2.326 ± 0.067	2.826ProLeu: 2.826 ± 0.072	0.729ProMet: 0.729 ± 0.032	1.715ProAsn: 1.715 ± 0.051	0.545ProPro: 0.545 ± 0.029	1.196ProGln: 1.196 ± 0.043	1.036ProArg: 1.036 ± 0.046	1.834ProSer: 1.834 ± 0.064	1.912ProThr: 1.912 ± 0.05	2.476ProVal: 2.476 ± 0.071	0.298ProTrp: 0.298 ± 0.022	1.262ProTyr: 1.262 ± 0.046	0.0ProXaa: 0.0 ± 0.0
Gln	2.75GlnAla: 2.75 ± 0.083	0.14GlnCys: 0.14 ± 0.016	1.936GlnAsp: 1.936 ± 0.064	2.617GlnGlu: 2.617 ± 0.071	1.295GlnPhe: 1.295 ± 0.051	2.024GlnGly: 2.024 ± 0.053	0.65GlnHis: 0.65 ± 0.034	2.727GlnIle: 2.727 ± 0.075	3.279GlnLys: 3.279 ± 0.082	3.562GlnLeu: 3.562 ± 0.096	1.031GlnMet: 1.031 ± 0.055	2.046GlnAsn: 2.046 ± 0.064	1.029GlnPro: 1.029 ± 0.046	1.608GlnGln: 1.608 ± 0.057	1.593GlnArg: 1.593 ± 0.049	1.938GlnSer: 1.938 ± 0.07	1.974GlnThr: 1.974 ± 0.067	2.534GlnVal: 2.534 ± 0.067	0.326GlnTrp: 0.326 ± 0.025	1.355GlnTyr: 1.355 ± 0.046	0.0GlnXaa: 0.0 ± 0.0
Arg	2.239ArgAla: 2.239 ± 0.066	0.174ArgCys: 0.174 ± 0.018	2.095ArgAsp: 2.095 ± 0.056	2.658ArgGlu: 2.658 ± 0.076	1.645ArgPhe: 1.645 ± 0.058	2.112ArgGly: 2.112 ± 0.07	0.76ArgHis: 0.76 ± 0.037	3.062ArgIle: 3.062 ± 0.082	3.477ArgLys: 3.477 ± 0.081	3.639ArgLeu: 3.639 ± 0.077	1.083ArgMet: 1.083 ± 0.046	2.158ArgAsn: 2.158 ± 0.067	1.215ArgPro: 1.215 ± 0.044	1.658ArgGln: 1.658 ± 0.061	1.936ArgArg: 1.936 ± 0.076	1.945ArgSer: 1.945 ± 0.056	1.874ArgThr: 1.874 ± 0.055	2.443ArgVal: 2.443 ± 0.06	0.331ArgTrp: 0.331 ± 0.023	1.691ArgTyr: 1.691 ± 0.057	0.0ArgXaa: 0.0 ± 0.0
Ser	3.627SerAla: 3.627 ± 0.092	0.312SerCys: 0.312 ± 0.026	3.593SerAsp: 3.593 ± 0.077	3.915SerGlu: 3.915 ± 0.09	2.712SerPhe: 2.712 ± 0.076	4.16SerGly: 4.16 ± 0.081	1.122SerHis: 1.122 ± 0.047	4.496SerIle: 4.496 ± 0.106	4.796SerLys: 4.796 ± 0.109	6.148SerLeu: 6.148 ± 0.107	1.469SerMet: 1.469 ± 0.049	3.251SerAsn: 3.251 ± 0.073	1.686SerPro: 1.686 ± 0.057	2.433SerGln: 2.433 ± 0.068	2.174SerArg: 2.174 ± 0.06	4.457SerSer: 4.457 ± 0.158	3.176SerThr: 3.176 ± 0.083	3.955SerVal: 3.955 ± 0.079	0.679SerTrp: 0.679 ± 0.039	2.446SerTyr: 2.446 ± 0.065	0.0SerXaa: 0.0 ± 0.0
Thr	3.619ThrAla: 3.619 ± 0.078	0.279ThrCys: 0.279 ± 0.022	3.157ThrAsp: 3.157 ± 0.07	3.229ThrGlu: 3.229 ± 0.081	2.483ThrPhe: 2.483 ± 0.075	4.043ThrGly: 4.043 ± 0.091	1.04ThrHis: 1.04 ± 0.042	4.377ThrIle: 4.377 ± 0.082	3.891ThrLys: 3.891 ± 0.097	5.381ThrLeu: 5.381 ± 0.107	1.202ThrMet: 1.202 ± 0.047	2.931ThrAsn: 2.931 ± 0.079	2.452ThrPro: 2.452 ± 0.074	1.771ThrGln: 1.771 ± 0.061	1.796ThrArg: 1.796 ± 0.062	3.436ThrSer: 3.436 ± 0.092	3.474ThrThr: 3.474 ± 0.123	4.136ThrVal: 4.136 ± 0.092	0.545ThrTrp: 0.545 ± 0.031	2.126ThrTyr: 2.126 ± 0.063	0.0ThrXaa: 0.0 ± 0.0
Val	5.288ValAla: 5.288 ± 0.102	0.457ValCys: 0.457 ± 0.028	4.207ValAsp: 4.207 ± 0.084	4.393ValGlu: 4.393 ± 0.101	2.62ValPhe: 2.62 ± 0.07	4.643ValGly: 4.643 ± 0.113	1.083ValHis: 1.083 ± 0.051	5.527ValIle: 5.527 ± 0.094	5.275ValLys: 5.275 ± 0.121	6.379ValLeu: 6.379 ± 0.111	1.634ValMet: 1.634 ± 0.051	3.539ValAsn: 3.539 ± 0.082	2.46ValPro: 2.46 ± 0.069	1.995ValGln: 1.995 ± 0.054	2.307ValArg: 2.307 ± 0.073	4.669ValSer: 4.669 ± 0.098	4.244ValThr: 4.244 ± 0.101	5.184ValVal: 5.184 ± 0.108	0.569ValTrp: 0.569 ± 0.027	2.484ValTyr: 2.484 ± 0.081	0.0ValXaa: 0.0 ± 0.0
Trp	0.505TrpAla: 0.505 ± 0.031	0.067TrpCys: 0.067 ± 0.011	0.488TrpAsp: 0.488 ± 0.031	0.486TrpGlu: 0.486 ± 0.031	0.474TrpPhe: 0.474 ± 0.028	0.615TrpGly: 0.615 ± 0.035	0.197TrpHis: 0.197 ± 0.02	0.8TrpIle: 0.8 ± 0.041	0.765TrpLys: 0.765 ± 0.039	1.198TrpLeu: 1.198 ± 0.052	0.281TrpMet: 0.281 ± 0.026	0.602TrpAsn: 0.602 ± 0.032	0.195TrpPro: 0.195 ± 0.019	0.484TrpGln: 0.484 ± 0.03	0.362TrpArg: 0.362 ± 0.027	0.515TrpSer: 0.515 ± 0.029	0.467TrpThr: 0.467 ± 0.03	0.572TrpVal: 0.572 ± 0.03	0.126TrpTrp: 0.126 ± 0.014	0.429TrpTyr: 0.429 ± 0.036	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.341TyrAla: 2.341 ± 0.072	0.314TyrCys: 0.314 ± 0.027	2.331TyrAsp: 2.331 ± 0.06	2.239TyrGlu: 2.239 ± 0.071	2.026TyrPhe: 2.026 ± 0.062	2.657TyrGly: 2.657 ± 0.072	0.85TyrHis: 0.85 ± 0.04	2.795TyrIle: 2.795 ± 0.063	2.395TyrLys: 2.395 ± 0.08	4.294TyrLeu: 4.294 ± 0.101	0.881TyrMet: 0.881 ± 0.038	1.996TyrAsn: 1.996 ± 0.065	1.5TyrPro: 1.5 ± 0.055	1.908TyrGln: 1.908 ± 0.063	1.857TyrArg: 1.857 ± 0.055	2.436TyrSer: 2.436 ± 0.065	2.121TyrThr: 2.121 ± 0.057	2.421TyrVal: 2.421 ± 0.061	0.414TyrTrp: 0.414 ± 0.026	1.926TyrTyr: 1.926 ± 0.088	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 1998 proteins (580046 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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