Lactobacillus equi DPC 6820
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2019 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|V7HTG1|V7HTG1_9LACO Phosphatidylglycerol lysyltransferase OS=Lactobacillus equi DPC 6820 OX=1392007 GN=mprF PE=3 SV=1
MM1 pKa = 7.71 KK2 pKa = 9.47 LTTDD6 pKa = 3.48 VEE8 pKa = 4.62 LYY10 pKa = 10.41 AYY12 pKa = 10.27 QEE14 pKa = 4.74 GYY16 pKa = 10.42 KK17 pKa = 10.07 EE18 pKa = 3.71 AARR21 pKa = 11.84 YY22 pKa = 8.77 SEE24 pKa = 4.46 KK25 pKa = 10.49 EE26 pKa = 3.78 LRR28 pKa = 11.84 DD29 pKa = 3.65 FKK31 pKa = 11.46 DD32 pKa = 2.92 WVDD35 pKa = 3.04 GGKK38 pKa = 10.44 YY39 pKa = 9.74 YY40 pKa = 10.46 EE41 pKa = 5.44 LEE43 pKa = 4.86 LEE45 pKa = 5.26 DD46 pKa = 4.15 IQDD49 pKa = 4.29 LLGSLIDD56 pKa = 4.02 SAADD60 pKa = 3.49 YY61 pKa = 10.82 AKK63 pKa = 10.71 NGPEE67 pKa = 4.02 SMQTEE72 pKa = 4.42 LTFEE76 pKa = 4.27 EE77 pKa = 4.88 DD78 pKa = 3.81 GKK80 pKa = 10.27 PEE82 pKa = 5.1 DD83 pKa = 3.25 ICEE86 pKa = 4.0 FCSFNGAPLLDD97 pKa = 5.05 DD98 pKa = 4.3 YY99 pKa = 11.93 GLTIYY104 pKa = 10.99 VDD106 pKa = 4.74 DD107 pKa = 4.45 AEE109 pKa = 4.2 KK110 pKa = 10.19 WLYY113 pKa = 9.82 VDD115 pKa = 4.13 KK116 pKa = 11.5 TEE118 pKa = 5.64 DD119 pKa = 3.24 TDD121 pKa = 3.67 KK122 pKa = 11.06 CIKK125 pKa = 10.24 INYY128 pKa = 9.1 CPMCGKK134 pKa = 10.04 EE135 pKa = 4.38 LGNDD139 pKa = 3.25
Molecular weight: 15.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.759
IPC2_protein 3.973
IPC_protein 3.948
Toseland 3.745
ProMoST 4.05
Dawson 3.923
Bjellqvist 4.101
Wikipedia 3.821
Rodwell 3.77
Grimsley 3.656
Solomon 3.91
Lehninger 3.872
Nozaki 4.024
DTASelect 4.228
Thurlkill 3.783
EMBOSS 3.834
Sillero 4.062
Patrickios 1.163
IPC_peptide 3.91
IPC2_peptide 4.037
IPC2.peptide.svr19 3.955
Protein with the highest isoelectric point:
>tr|V7HUB1|V7HUB1_9LACO ATP-dependent helicase/nuclease subunit A OS=Lactobacillus equi DPC 6820 OX=1392007 GN=addA PE=3 SV=1
MM1 pKa = 7.57 CGIPYY6 pKa = 9.38 VCRR9 pKa = 11.84 GGARR13 pKa = 11.84 PKK15 pKa = 10.68 RR16 pKa = 11.84 LIAWVLRR23 pKa = 11.84 IPYY26 pKa = 9.39 VCRR29 pKa = 11.84 CGPLSAQNFSNHH41 pKa = 5.43 KK42 pKa = 9.14 KK43 pKa = 10.08 VFPIRR48 pKa = 11.84 IGAPYY53 pKa = 9.67 GGKK56 pKa = 8.13 ITTAYY61 pKa = 10.68 VEE63 pKa = 4.14
Molecular weight: 7.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.306
IPC2_protein 9.619
IPC_protein 9.984
Toseland 10.028
ProMoST 9.838
Dawson 10.292
Bjellqvist 10.058
Wikipedia 10.496
Rodwell 10.555
Grimsley 10.379
Solomon 10.335
Lehninger 10.292
Nozaki 10.16
DTASelect 10.014
Thurlkill 10.131
EMBOSS 10.452
Sillero 10.233
Patrickios 10.204
IPC_peptide 10.321
IPC2_peptide 9.355
IPC2.peptide.svr19 8.295
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2019
0
2019
585827
14
2801
290.2
32.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.143 ± 0.064
0.571 ± 0.014
5.66 ± 0.052
6.198 ± 0.056
4.246 ± 0.043
6.491 ± 0.05
1.857 ± 0.024
6.604 ± 0.055
6.907 ± 0.059
10.069 ± 0.066
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.596 ± 0.027
4.9 ± 0.055
3.326 ± 0.032
4.936 ± 0.059
4.018 ± 0.042
5.789 ± 0.054
5.743 ± 0.045
7.111 ± 0.044
0.983 ± 0.02
3.852 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here