Amino acid dipepetide frequency for Lactobacillus equi DPC 6820

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.202AlaAla: 6.202 ± 0.143
0.533AlaCys: 0.533 ± 0.03
4.693AlaAsp: 4.693 ± 0.097
4.481AlaGlu: 4.481 ± 0.112
3.26AlaPhe: 3.26 ± 0.097
5.706AlaGly: 5.706 ± 0.115
1.558AlaHis: 1.558 ± 0.055
5.302AlaIle: 5.302 ± 0.124
6.068AlaLys: 6.068 ± 0.119
8.318AlaLeu: 8.318 ± 0.147
2.153AlaMet: 2.153 ± 0.065
3.945AlaAsn: 3.945 ± 0.089
2.24AlaPro: 2.24 ± 0.06
5.169AlaGln: 5.169 ± 0.148
2.963AlaArg: 2.963 ± 0.074
4.682AlaSer: 4.682 ± 0.102
4.858AlaThr: 4.858 ± 0.099
5.348AlaVal: 5.348 ± 0.107
0.789AlaTrp: 0.789 ± 0.041
3.161AlaTyr: 3.161 ± 0.073
0.0AlaXaa: 0.0 ± 0.0
Cys
0.434CysAla: 0.434 ± 0.031
0.055CysCys: 0.055 ± 0.012
0.365CysAsp: 0.365 ± 0.025
0.263CysGlu: 0.263 ± 0.02
0.251CysPhe: 0.251 ± 0.02
0.527CysGly: 0.527 ± 0.028
0.183CysHis: 0.183 ± 0.018
0.271CysIle: 0.271 ± 0.02
0.203CysLys: 0.203 ± 0.022
0.662CysLeu: 0.662 ± 0.035
0.106CysMet: 0.106 ± 0.012
0.198CysAsn: 0.198 ± 0.019
0.253CysPro: 0.253 ± 0.028
0.437CysGln: 0.437 ± 0.029
0.266CysArg: 0.266 ± 0.022
0.319CysSer: 0.319 ± 0.024
0.239CysThr: 0.239 ± 0.02
0.326CysVal: 0.326 ± 0.025
0.068CysTrp: 0.068 ± 0.011
0.285CysTyr: 0.285 ± 0.026
0.0CysXaa: 0.0 ± 0.0
Asp
3.984AspAla: 3.984 ± 0.089
0.391AspCys: 0.391 ± 0.026
3.312AspAsp: 3.312 ± 0.083
4.08AspGlu: 4.08 ± 0.103
3.274AspPhe: 3.274 ± 0.074
3.527AspGly: 3.527 ± 0.086
0.949AspHis: 0.949 ± 0.038
3.614AspIle: 3.614 ± 0.079
3.689AspLys: 3.689 ± 0.09
6.079AspLeu: 6.079 ± 0.109
1.466AspMet: 1.466 ± 0.051
2.68AspAsn: 2.68 ± 0.083
1.804AspPro: 1.804 ± 0.055
2.7AspGln: 2.7 ± 0.072
2.076AspArg: 2.076 ± 0.061
2.996AspSer: 2.996 ± 0.077
2.632AspThr: 2.632 ± 0.061
3.871AspVal: 3.871 ± 0.089
0.667AspTrp: 0.667 ± 0.037
2.811AspTyr: 2.811 ± 0.077
0.0AspXaa: 0.0 ± 0.0
Glu
5.061GluAla: 5.061 ± 0.108
0.263GluCys: 0.263 ± 0.022
3.211GluAsp: 3.211 ± 0.081
4.814GluGlu: 4.814 ± 0.137
2.463GluPhe: 2.463 ± 0.069
3.086GluGly: 3.086 ± 0.085
1.021GluHis: 1.021 ± 0.034
4.933GluIle: 4.933 ± 0.11
5.198GluLys: 5.198 ± 0.117
6.323GluLeu: 6.323 ± 0.108
1.82GluMet: 1.82 ± 0.057
3.508GluAsn: 3.508 ± 0.092
1.347GluPro: 1.347 ± 0.055
2.549GluGln: 2.549 ± 0.066
2.533GluArg: 2.533 ± 0.076
3.069GluSer: 3.069 ± 0.103
3.156GluThr: 3.156 ± 0.091
4.959GluVal: 4.959 ± 0.11
0.57GluTrp: 0.57 ± 0.03
2.093GluTyr: 2.093 ± 0.07
0.0GluXaa: 0.0 ± 0.0
Phe
3.216PheAla: 3.216 ± 0.091
0.304PheCys: 0.304 ± 0.025
2.88PheAsp: 2.88 ± 0.077
2.569PheGlu: 2.569 ± 0.074
1.978PhePhe: 1.978 ± 0.078
3.032PheGly: 3.032 ± 0.084
0.71PheHis: 0.71 ± 0.043
2.902PheIle: 2.902 ± 0.088
2.726PheLys: 2.726 ± 0.068
4.071PheLeu: 4.071 ± 0.11
1.161PheMet: 1.161 ± 0.051
2.26PheAsn: 2.26 ± 0.067
1.321PhePro: 1.321 ± 0.051
1.434PheGln: 1.434 ± 0.052
1.422PheArg: 1.422 ± 0.051
2.758PheSer: 2.758 ± 0.085
2.472PheThr: 2.472 ± 0.067
2.924PheVal: 2.924 ± 0.082
0.548PheTrp: 0.548 ± 0.033
1.774PheTyr: 1.774 ± 0.06
0.0PheXaa: 0.0 ± 0.0
Gly
4.667GlyAla: 4.667 ± 0.116
0.411GlyCys: 0.411 ± 0.029
3.301GlyAsp: 3.301 ± 0.09
3.172GlyGlu: 3.172 ± 0.082
2.974GlyPhe: 2.974 ± 0.072
4.259GlyGly: 4.259 ± 0.12
1.451GlyHis: 1.451 ± 0.058
4.993GlyIle: 4.993 ± 0.117
4.611GlyLys: 4.611 ± 0.079
6.726GlyLeu: 6.726 ± 0.137
1.999GlyMet: 1.999 ± 0.063
2.805GlyAsn: 2.805 ± 0.085
1.596GlyPro: 1.596 ± 0.053
3.755GlyGln: 3.755 ± 0.083
2.704GlyArg: 2.704 ± 0.077
3.632GlySer: 3.632 ± 0.085
3.571GlyThr: 3.571 ± 0.093
4.682GlyVal: 4.682 ± 0.098
0.727GlyTrp: 0.727 ± 0.047
2.875GlyTyr: 2.875 ± 0.066
0.0GlyXaa: 0.0 ± 0.0
His
1.27HisAla: 1.27 ± 0.053
0.123HisCys: 0.123 ± 0.014
1.022HisAsp: 1.022 ± 0.041
1.055HisGlu: 1.055 ± 0.046
0.932HisPhe: 0.932 ± 0.038
1.41HisGly: 1.41 ± 0.055
0.562HisHis: 0.562 ± 0.033
1.028HisIle: 1.028 ± 0.049
1.04HisLys: 1.04 ± 0.04
1.949HisLeu: 1.949 ± 0.062
0.399HisMet: 0.399 ± 0.026
0.918HisAsn: 0.918 ± 0.045
0.954HisPro: 0.954 ± 0.043
1.045HisGln: 1.045 ± 0.048
0.804HisArg: 0.804 ± 0.036
0.941HisSer: 0.941 ± 0.041
0.884HisThr: 0.884 ± 0.036
1.2HisVal: 1.2 ± 0.041
0.162HisTrp: 0.162 ± 0.018
0.872HisTyr: 0.872 ± 0.039
0.0HisXaa: 0.0 ± 0.0
Ile
5.597IleAla: 5.597 ± 0.099
0.504IleCys: 0.504 ± 0.032
3.906IleAsp: 3.906 ± 0.084
3.629IleGlu: 3.629 ± 0.089
3.124IlePhe: 3.124 ± 0.093
4.353IleGly: 4.353 ± 0.112
1.118IleHis: 1.118 ± 0.045
4.554IleIle: 4.554 ± 0.118
4.436IleLys: 4.436 ± 0.092
6.458IleLeu: 6.458 ± 0.133
1.702IleMet: 1.702 ± 0.055
3.317IleAsn: 3.317 ± 0.092
2.549IlePro: 2.549 ± 0.069
2.431IleGln: 2.431 ± 0.069
2.492IleArg: 2.492 ± 0.063
4.38IleSer: 4.38 ± 0.085
3.94IleThr: 3.94 ± 0.091
4.539IleVal: 4.539 ± 0.109
0.574IleTrp: 0.574 ± 0.035
2.436IleTyr: 2.436 ± 0.063
0.0IleXaa: 0.0 ± 0.0
Lys
5.331LysAla: 5.331 ± 0.103
0.208LysCys: 0.208 ± 0.019
3.829LysAsp: 3.829 ± 0.088
5.109LysGlu: 5.109 ± 0.107
2.269LysPhe: 2.269 ± 0.062
3.547LysGly: 3.547 ± 0.085
1.168LysHis: 1.168 ± 0.049
4.809LysIle: 4.809 ± 0.083
5.481LysLys: 5.481 ± 0.124
6.678LysLeu: 6.678 ± 0.118
2.211LysMet: 2.211 ± 0.058
3.772LysAsn: 3.772 ± 0.097
2.052LysPro: 2.052 ± 0.067
3.564LysGln: 3.564 ± 0.095
3.252LysArg: 3.252 ± 0.079
3.51LysSer: 3.51 ± 0.067
3.982LysThr: 3.982 ± 0.082
5.097LysVal: 5.097 ± 0.106
0.604LysTrp: 0.604 ± 0.032
2.595LysTyr: 2.595 ± 0.077
0.0LysXaa: 0.0 ± 0.0
Leu
10.005LeuAla: 10.005 ± 0.138
0.556LeuCys: 0.556 ± 0.03
5.679LeuAsp: 5.679 ± 0.089
6.261LeuGlu: 6.261 ± 0.11
3.767LeuPhe: 3.767 ± 0.104
7.087LeuGly: 7.087 ± 0.127
1.663LeuHis: 1.663 ± 0.051
6.193LeuIle: 6.193 ± 0.121
6.758LeuLys: 6.758 ± 0.119
9.365LeuLeu: 9.365 ± 0.193
2.458LeuMet: 2.458 ± 0.071
4.899LeuAsn: 4.899 ± 0.091
3.918LeuPro: 3.918 ± 0.072
4.087LeuGln: 4.087 ± 0.086
3.972LeuArg: 3.972 ± 0.09
6.359LeuSer: 6.359 ± 0.121
6.536LeuThr: 6.536 ± 0.122
7.219LeuVal: 7.219 ± 0.124
0.884LeuTrp: 0.884 ± 0.036
3.026LeuTyr: 3.026 ± 0.078
0.0LeuXaa: 0.0 ± 0.0
Met
2.328MetAla: 2.328 ± 0.062
0.157MetCys: 0.157 ± 0.017
1.379MetAsp: 1.379 ± 0.048
1.594MetGlu: 1.594 ± 0.055
0.966MetPhe: 0.966 ± 0.044
1.746MetGly: 1.746 ± 0.057
0.417MetHis: 0.417 ± 0.024
1.763MetIle: 1.763 ± 0.067
1.709MetLys: 1.709 ± 0.054
2.537MetLeu: 2.537 ± 0.067
0.734MetMet: 0.734 ± 0.038
1.147MetAsn: 1.147 ± 0.05
0.983MetPro: 0.983 ± 0.043
1.168MetGln: 1.168 ± 0.044
1.099MetArg: 1.099 ± 0.038
1.572MetSer: 1.572 ± 0.045
1.763MetThr: 1.763 ± 0.059
1.972MetVal: 1.972 ± 0.06
0.2MetTrp: 0.2 ± 0.019
0.725MetTyr: 0.725 ± 0.035
0.0MetXaa: 0.0 ± 0.0
Asn
3.31AsnAla: 3.31 ± 0.077
0.253AsnCys: 0.253 ± 0.021
2.811AsnAsp: 2.811 ± 0.083
2.781AsnGlu: 2.781 ± 0.08
2.362AsnPhe: 2.362 ± 0.074
3.376AsnGly: 3.376 ± 0.088
1.132AsnHis: 1.132 ± 0.045
3.025AsnIle: 3.025 ± 0.07
2.987AsnLys: 2.987 ± 0.093
5.107AsnLeu: 5.107 ± 0.1
1.239AsnMet: 1.239 ± 0.042
2.513AsnAsn: 2.513 ± 0.084
2.204AsnPro: 2.204 ± 0.069
2.909AsnGln: 2.909 ± 0.074
2.139AsnArg: 2.139 ± 0.065
2.629AsnSer: 2.629 ± 0.087
2.368AsnThr: 2.368 ± 0.074
2.927AsnVal: 2.927 ± 0.084
0.594AsnTrp: 0.594 ± 0.036
2.333AsnTyr: 2.333 ± 0.062
0.0AsnXaa: 0.0 ± 0.0
Pro
2.743ProAla: 2.743 ± 0.08
0.14ProCys: 0.14 ± 0.015
1.963ProAsp: 1.963 ± 0.07
2.733ProGlu: 2.733 ± 0.07
1.473ProPhe: 1.473 ± 0.045
2.038ProGly: 2.038 ± 0.073
0.681ProHis: 0.681 ± 0.037
1.963ProIle: 1.963 ± 0.057
2.274ProLys: 2.274 ± 0.054
3.19ProLeu: 3.19 ± 0.078
0.671ProMet: 0.671 ± 0.035
1.635ProAsn: 1.635 ± 0.063
0.591ProPro: 0.591 ± 0.034
1.678ProGln: 1.678 ± 0.051
1.123ProArg: 1.123 ± 0.044
2.106ProSer: 2.106 ± 0.124
2.014ProThr: 2.014 ± 0.061
2.639ProVal: 2.639 ± 0.073
0.28ProTrp: 0.28 ± 0.022
1.328ProTyr: 1.328 ± 0.048
0.0ProXaa: 0.0 ± 0.0
Gln
5.85GlnAla: 5.85 ± 0.139
0.135GlnCys: 0.135 ± 0.016
2.562GlnAsp: 2.562 ± 0.076
3.557GlnGlu: 3.557 ± 0.081
1.347GlnPhe: 1.347 ± 0.047
2.95GlnGly: 2.95 ± 0.066
0.806GlnHis: 0.806 ± 0.034
3.184GlnIle: 3.184 ± 0.093
4.064GlnLys: 4.064 ± 0.089
4.53GlnLeu: 4.53 ± 0.106
1.209GlnMet: 1.209 ± 0.047
2.403GlnAsn: 2.403 ± 0.069
1.465GlnPro: 1.465 ± 0.047
1.874GlnGln: 1.874 ± 0.069
1.992GlnArg: 1.992 ± 0.068
2.501GlnSer: 2.501 ± 0.067
2.979GlnThr: 2.979 ± 0.079
4.087GlnVal: 4.087 ± 0.105
0.381GlnTrp: 0.381 ± 0.029
1.49GlnTyr: 1.49 ± 0.052
0.0GlnXaa: 0.0 ± 0.0
Arg
2.702ArgAla: 2.702 ± 0.07
0.167ArgCys: 0.167 ± 0.017
2.159ArgAsp: 2.159 ± 0.062
2.465ArgGlu: 2.465 ± 0.073
1.777ArgPhe: 1.777 ± 0.051
2.282ArgGly: 2.282 ± 0.065
0.807ArgHis: 0.807 ± 0.039
2.648ArgIle: 2.648 ± 0.068
2.984ArgLys: 2.984 ± 0.071
4.168ArgLeu: 4.168 ± 0.095
1.089ArgMet: 1.089 ± 0.044
1.978ArgAsn: 1.978 ± 0.062
1.393ArgPro: 1.393 ± 0.045
2.685ArgGln: 2.685 ± 0.064
2.052ArgArg: 2.052 ± 0.073
1.961ArgSer: 1.961 ± 0.056
1.997ArgThr: 1.997 ± 0.056
2.634ArgVal: 2.634 ± 0.077
0.365ArgTrp: 0.365 ± 0.027
1.861ArgTyr: 1.861 ± 0.061
0.0ArgXaa: 0.0 ± 0.0
Ser
4.102SerAla: 4.102 ± 0.097
0.297SerCys: 0.297 ± 0.022
3.31SerAsp: 3.31 ± 0.092
3.711SerGlu: 3.711 ± 0.152
2.74SerPhe: 2.74 ± 0.074
4.09SerGly: 4.09 ± 0.096
1.13SerHis: 1.13 ± 0.046
3.166SerIle: 3.166 ± 0.071
3.803SerLys: 3.803 ± 0.09
6.234SerLeu: 6.234 ± 0.114
1.378SerMet: 1.378 ± 0.047
2.639SerAsn: 2.639 ± 0.082
1.866SerPro: 1.866 ± 0.059
3.482SerGln: 3.482 ± 0.095
2.492SerArg: 2.492 ± 0.066
3.696SerSer: 3.696 ± 0.102
2.893SerThr: 2.893 ± 0.077
3.902SerVal: 3.902 ± 0.088
0.669SerTrp: 0.669 ± 0.036
2.291SerTyr: 2.291 ± 0.06
0.0SerXaa: 0.0 ± 0.0
Thr
4.506ThrAla: 4.506 ± 0.102
0.311ThrCys: 0.311 ± 0.023
3.132ThrAsp: 3.132 ± 0.082
3.059ThrGlu: 3.059 ± 0.086
2.489ThrPhe: 2.489 ± 0.066
4.022ThrGly: 4.022 ± 0.083
1.016ThrHis: 1.016 ± 0.04
3.892ThrIle: 3.892 ± 0.083
3.926ThrLys: 3.926 ± 0.075
5.783ThrLeu: 5.783 ± 0.116
1.393ThrMet: 1.393 ± 0.05
2.762ThrAsn: 2.762 ± 0.077
2.502ThrPro: 2.502 ± 0.072
2.294ThrGln: 2.294 ± 0.065
1.905ThrArg: 1.905 ± 0.061
3.547ThrSer: 3.547 ± 0.09
3.764ThrThr: 3.764 ± 0.115
4.32ThrVal: 4.32 ± 0.093
0.572ThrTrp: 0.572 ± 0.031
2.238ThrTyr: 2.238 ± 0.064
0.0ThrXaa: 0.0 ± 0.0
Val
6.695ValAla: 6.695 ± 0.121
0.556ValCys: 0.556 ± 0.032
4.264ValAsp: 4.264 ± 0.084
4.247ValGlu: 4.247 ± 0.097
2.682ValPhe: 2.682 ± 0.073
4.689ValGly: 4.689 ± 0.107
1.07ValHis: 1.07 ± 0.046
5.058ValIle: 5.058 ± 0.105
4.831ValLys: 4.831 ± 0.094
6.717ValLeu: 6.717 ± 0.11
1.693ValMet: 1.693 ± 0.06
3.335ValAsn: 3.335 ± 0.073
2.41ValPro: 2.41 ± 0.063
2.709ValGln: 2.709 ± 0.066
2.552ValArg: 2.552 ± 0.072
4.512ValSer: 4.512 ± 0.095
4.757ValThr: 4.757 ± 0.104
5.437ValVal: 5.437 ± 0.109
0.611ValTrp: 0.611 ± 0.036
2.282ValTyr: 2.282 ± 0.064
0.0ValXaa: 0.0 ± 0.0
Trp
0.609TrpAla: 0.609 ± 0.032
0.077TrpCys: 0.077 ± 0.011
0.587TrpAsp: 0.587 ± 0.035
0.471TrpGlu: 0.471 ± 0.032
0.468TrpPhe: 0.468 ± 0.028
0.662TrpGly: 0.662 ± 0.034
0.258TrpHis: 0.258 ± 0.028
0.601TrpIle: 0.601 ± 0.034
0.519TrpLys: 0.519 ± 0.03
1.268TrpLeu: 1.268 ± 0.053
0.271TrpMet: 0.271 ± 0.021
0.442TrpAsn: 0.442 ± 0.027
0.28TrpPro: 0.28 ± 0.022
0.7TrpGln: 0.7 ± 0.037
0.393TrpArg: 0.393 ± 0.027
0.606TrpSer: 0.606 ± 0.035
0.51TrpThr: 0.51 ± 0.033
0.609TrpVal: 0.609 ± 0.035
0.149TrpTrp: 0.149 ± 0.018
0.35TrpTyr: 0.35 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.817TyrAla: 2.817 ± 0.074
0.271TyrCys: 0.271 ± 0.024
2.236TyrAsp: 2.236 ± 0.062
1.925TyrGlu: 1.925 ± 0.061
1.866TyrPhe: 1.866 ± 0.061
2.52TyrGly: 2.52 ± 0.061
0.872TyrHis: 0.872 ± 0.042
2.141TyrIle: 2.141 ± 0.065
1.76TyrLys: 1.76 ± 0.061
4.529TyrLeu: 4.529 ± 0.103
0.807TyrMet: 0.807 ± 0.035
1.842TyrAsn: 1.842 ± 0.069
1.538TyrPro: 1.538 ± 0.05
2.695TyrGln: 2.695 ± 0.078
1.944TyrArg: 1.944 ± 0.06
2.115TyrSer: 2.115 ± 0.06
2.117TyrThr: 2.117 ± 0.065
2.415TyrVal: 2.415 ± 0.065
0.417TyrTrp: 0.417 ± 0.03
1.692TyrTyr: 1.692 ± 0.064
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2019 proteins (585827 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski