Xanthomonas phage Pagan

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Pradovirus; unclassified Pradovirus

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B9NE07|A0A5B9NE07_9CAUD O-spanin OS=Xanthomonas phage Pagan OX=2591104 GN=CPT_Pagan_058 PE=4 SV=1
MM1 pKa = 7.62AVPDD5 pKa = 4.03PQLRR9 pKa = 11.84YY10 pKa = 8.97ATDD13 pKa = 3.14VFDD16 pKa = 5.53ADD18 pKa = 4.57GVTTDD23 pKa = 3.28WQISFTGGYY32 pKa = 9.41INPSHH37 pKa = 6.79VYY39 pKa = 10.73AMSGLLDD46 pKa = 4.23EE47 pKa = 4.81EE48 pKa = 4.78TQLLTDD54 pKa = 4.31RR55 pKa = 11.84TPHH58 pKa = 4.77TVEE61 pKa = 4.43VLSEE65 pKa = 4.69DD66 pKa = 4.26DD67 pKa = 3.73DD68 pKa = 5.62ASTVRR73 pKa = 11.84VSPAVAAGRR82 pKa = 11.84KK83 pKa = 9.05LYY85 pKa = 9.94IYY87 pKa = 10.25RR88 pKa = 11.84STPVQQMLVDD98 pKa = 3.85YY99 pKa = 10.22VNGSIISKK107 pKa = 9.44TNLNLSNDD115 pKa = 3.58QLLKK119 pKa = 10.39IIQEE123 pKa = 4.34MFDD126 pKa = 3.67SLNIATLSIDD136 pKa = 3.35QQVGVIVDD144 pKa = 4.16LNEE147 pKa = 4.43IIQNIYY153 pKa = 9.87KK154 pKa = 10.12QVTEE158 pKa = 4.35LLAAGGIVSVAPRR171 pKa = 11.84VWSGAWTGDD180 pKa = 3.52QVDD183 pKa = 4.08DD184 pKa = 4.18TDD186 pKa = 4.46FDD188 pKa = 4.03MVGADD193 pKa = 3.27VSGAGFYY200 pKa = 10.67DD201 pKa = 4.08VYY203 pKa = 11.61VNGIGMQPDD212 pKa = 2.79VDD214 pKa = 4.18YY215 pKa = 11.27QVTLADD221 pKa = 3.87DD222 pKa = 4.01TDD224 pKa = 3.84PSFIRR229 pKa = 11.84FATVPAEE236 pKa = 3.82GSIWFAVLRR245 pKa = 11.84GYY247 pKa = 10.59AKK249 pKa = 10.11PYY251 pKa = 9.02TGPAPITATSLRR263 pKa = 11.84VPIIPAEE270 pKa = 4.31GPTYY274 pKa = 10.52YY275 pKa = 10.68ADD277 pKa = 3.52KK278 pKa = 9.47ATEE281 pKa = 4.03YY282 pKa = 11.28GLVRR286 pKa = 11.84CTYY289 pKa = 11.1AGGCAVNINLIPEE302 pKa = 4.32VGDD305 pKa = 3.95GEE307 pKa = 4.63TKK309 pKa = 10.39LASGSYY315 pKa = 10.34FSVQQKK321 pKa = 10.22AGPVVITGDD330 pKa = 3.32VGVTLEE336 pKa = 4.26VPVGCTAATRR346 pKa = 11.84GTNSVVTATCIDD358 pKa = 3.65GDD360 pKa = 4.23TNTWLLSGDD369 pKa = 3.9LAKK372 pKa = 10.54EE373 pKa = 3.9

Molecular weight: 39.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B9N6E2|A0A5B9N6E2_9CAUD Uncharacterized protein OS=Xanthomonas phage Pagan OX=2591104 GN=CPT_Pagan_015 PE=4 SV=1
MM1 pKa = 7.45IRR3 pKa = 11.84FKK5 pKa = 10.84FSRR8 pKa = 11.84HH9 pKa = 3.77YY10 pKa = 11.04VEE12 pKa = 5.32AARR15 pKa = 11.84DD16 pKa = 3.73SYY18 pKa = 11.68NRR20 pKa = 11.84AILKK24 pKa = 10.16HH25 pKa = 5.54PAGSVSALYY34 pKa = 9.98ILPCGGRR41 pKa = 11.84SFDD44 pKa = 4.0IRR46 pKa = 11.84LAA48 pKa = 3.82

Molecular weight: 5.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

14405

41

1636

244.2

26.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.837 ± 0.617

1.0 ± 0.191

6.234 ± 0.177

5.699 ± 0.267

3.249 ± 0.149

8.101 ± 0.308

1.93 ± 0.186

4.45 ± 0.158

4.998 ± 0.356

8.108 ± 0.207

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.652 ± 0.181

4.165 ± 0.208

4.276 ± 0.246

4.276 ± 0.225

6.276 ± 0.311

5.609 ± 0.33

6.192 ± 0.291

6.831 ± 0.304

1.722 ± 0.129

3.395 ± 0.186
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski