Collinsella stercoris DSM 13279
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2527 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B6GA64|B6GA64_9ACTN Toxin-antitoxin system antitoxin component HicB family OS=Collinsella stercoris DSM 13279 OX=445975 GN=COLSTE_00959 PE=4 SV=1
MM1 pKa = 7.09 PQHH4 pKa = 6.6 RR5 pKa = 11.84 NKK7 pKa = 10.82 AVVAGLAVTLAFGGVALPAVASTEE31 pKa = 3.99 QAPATDD37 pKa = 3.85 SDD39 pKa = 4.56 PNGVGDD45 pKa = 4.85 PAPADD50 pKa = 3.69 YY51 pKa = 11.2 DD52 pKa = 3.5 KK53 pKa = 11.66 ADD55 pKa = 3.77 YY56 pKa = 11.4 YY57 pKa = 11.14 EE58 pKa = 4.4 GTEE61 pKa = 4.3 GVATFASARR70 pKa = 11.84 SASTLSPVSLSDD82 pKa = 3.13 EE83 pKa = 3.92 MKK85 pKa = 10.77 YY86 pKa = 8.24 FTKK89 pKa = 10.87 YY90 pKa = 10.74 EE91 pKa = 4.17 SGCDD95 pKa = 3.54 YY96 pKa = 11.44 DD97 pKa = 5.28 KK98 pKa = 11.54 GLSYY102 pKa = 11.55 GDD104 pKa = 4.54 GYY106 pKa = 11.64 NAMGYY111 pKa = 8.13 YY112 pKa = 9.84 QFDD115 pKa = 3.2 RR116 pKa = 11.84 RR117 pKa = 11.84 YY118 pKa = 9.89 SLLPFIEE125 pKa = 4.0 QVYY128 pKa = 10.17 GYY130 pKa = 10.84 NSTKK134 pKa = 10.53 YY135 pKa = 11.11 DD136 pKa = 3.51 MLRR139 pKa = 11.84 AALAYY144 pKa = 10.1 RR145 pKa = 11.84 DD146 pKa = 3.85 VLQNPSYY153 pKa = 11.37 EE154 pKa = 4.27 MYY156 pKa = 10.36 DD157 pKa = 3.38 YY158 pKa = 11.11 TSRR161 pKa = 11.84 QLSAPAQILNGAWHH175 pKa = 7.16 AAYY178 pKa = 9.87 AADD181 pKa = 3.84 PAEE184 pKa = 4.75 FSALQDD190 pKa = 3.16 SYY192 pKa = 12.07 AYY194 pKa = 10.53 NNYY197 pKa = 8.97 YY198 pKa = 10.8 LPVQSSLLNTYY209 pKa = 10.16 GVDD212 pKa = 3.22 VRR214 pKa = 11.84 DD215 pKa = 4.05 RR216 pKa = 11.84 ADD218 pKa = 3.5 CVKK221 pKa = 10.6 GLVWGMCNLFGQGGVQKK238 pKa = 10.17 FFKK241 pKa = 10.4 GANIDD246 pKa = 3.2 NSMTDD251 pKa = 3.18 RR252 pKa = 11.84 EE253 pKa = 5.27 LITALCDD260 pKa = 3.54 TVVEE264 pKa = 4.92 YY265 pKa = 11.38 VDD267 pKa = 4.42 DD268 pKa = 4.82 WYY270 pKa = 10.38 PSQPQYY276 pKa = 10.04 WDD278 pKa = 2.85 GWKK281 pKa = 10.03 NRR283 pKa = 11.84 YY284 pKa = 9.14 KK285 pKa = 10.52 KK286 pKa = 10.68 EE287 pKa = 3.84 KK288 pKa = 8.66 ATCLAYY294 pKa = 9.64 MDD296 pKa = 4.05 QHH298 pKa = 7.08 DD299 pKa = 4.75 AEE301 pKa = 4.55 QNANGQGQTDD311 pKa = 3.93 DD312 pKa = 4.05 QPNADD317 pKa = 4.82 EE318 pKa = 5.23 PGQDD322 pKa = 3.87 DD323 pKa = 4.2 SGAGLPIAPDD333 pKa = 3.44 APNGSEE339 pKa = 4.01 GQNGDD344 pKa = 3.32 GGGSQGDD351 pKa = 3.7 ADD353 pKa = 4.1 SGEE356 pKa = 4.3 SGSDD360 pKa = 2.68 GGAAHH365 pKa = 6.97 GGDD368 pKa = 3.93 SGSDD372 pKa = 3.35 GAVTPEE378 pKa = 4.06 GGQGSDD384 pKa = 3.22 AEE386 pKa = 4.31 QNAGGSQNSDD396 pKa = 3.79 DD397 pKa = 5.28 DD398 pKa = 6.26 ADD400 pKa = 4.19 DD401 pKa = 4.47 APNASVPDD409 pKa = 4.13 GNAGQGSSGDD419 pKa = 3.54 QEE421 pKa = 4.26 NAGGSGSGSAGNSGSAQGGTGNNGGSTQDD450 pKa = 3.35 GSAGTGNSAGTDD462 pKa = 3.24 GSTGNGSTQDD472 pKa = 3.28 TTTGGGSTHH481 pKa = 6.84 AGEE484 pKa = 4.84 GQGSSDD490 pKa = 5.43 DD491 pKa = 4.07 EE492 pKa = 4.26 QTDD495 pKa = 3.14 ASTGDD500 pKa = 3.66 EE501 pKa = 4.13 EE502 pKa = 6.54 DD503 pKa = 4.89 DD504 pKa = 4.67 GKK506 pKa = 8.19 TTPKK510 pKa = 10.82 DD511 pKa = 3.15 PAPQQNTTRR520 pKa = 11.84 DD521 pKa = 3.61 PMNSLGTTGDD531 pKa = 3.48 KK532 pKa = 7.71 TTSGQGDD539 pKa = 4.0 GAQGTSASGAGKK551 pKa = 8.6 ATAGGLPGTGDD562 pKa = 3.34 IATMVLTGAAGLAVAGSSFLSLAKK586 pKa = 10.15 KK587 pKa = 9.67 EE588 pKa = 4.0 RR589 pKa = 11.84 AGVDD593 pKa = 3.54 EE594 pKa = 4.83 SADD597 pKa = 3.9 SNDD600 pKa = 3.36 EE601 pKa = 3.96
Molecular weight: 61.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.687
IPC2_protein 3.808
IPC_protein 3.859
Toseland 3.617
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.024
Wikipedia 3.808
Rodwell 3.681
Grimsley 3.528
Solomon 3.859
Lehninger 3.808
Nozaki 3.961
DTASelect 4.253
Thurlkill 3.681
EMBOSS 3.808
Sillero 3.986
Patrickios 1.507
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.866
Protein with the highest isoelectric point:
>tr|B6GD79|B6GD79_9ACTN Serine-type D-Ala-D-Ala carboxypeptidase OS=Collinsella stercoris DSM 13279 OX=445975 GN=COLSTE_02057 PE=3 SV=1
MM1 pKa = 7.52 HH2 pKa = 7.32 GGAARR7 pKa = 11.84 PIGLGVGARR16 pKa = 11.84 AFSPKK21 pKa = 9.83 RR22 pKa = 11.84 PRR24 pKa = 11.84 LHH26 pKa = 6.76 AWRR29 pKa = 11.84 PCAGVFFSFLGRR41 pKa = 11.84 GRR43 pKa = 11.84 SIRR46 pKa = 11.84 VRR48 pKa = 3.4
Molecular weight: 5.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.363
IPC2_protein 10.774
IPC_protein 12.384
Toseland 12.544
ProMoST 13.056
Dawson 12.544
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.106
Grimsley 12.588
Solomon 13.042
Lehninger 12.939
Nozaki 12.544
DTASelect 12.544
Thurlkill 12.544
EMBOSS 13.042
Sillero 12.544
Patrickios 11.857
IPC_peptide 13.042
IPC2_peptide 12.032
IPC2.peptide.svr19 9.168
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2527
0
2527
703773
39
2718
278.5
30.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.725 ± 0.075
1.59 ± 0.021
6.19 ± 0.045
6.598 ± 0.051
3.612 ± 0.036
8.324 ± 0.045
2.022 ± 0.024
5.025 ± 0.04
3.546 ± 0.046
9.099 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.77 ± 0.028
2.872 ± 0.034
4.439 ± 0.036
2.849 ± 0.032
6.566 ± 0.065
5.849 ± 0.042
5.269 ± 0.041
7.826 ± 0.05
1.095 ± 0.02
2.736 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here