Absidia repens
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14767 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1X2I7U2|A0A1X2I7U2_9FUNG CUE domain-containing protein OS=Absidia repens OX=90262 GN=BCR42DRAFT_421547 PE=4 SV=1
MM1 pKa = 7.38 TSAFNSGIFKK11 pKa = 9.72 TLVEE15 pKa = 4.31 ANQRR19 pKa = 11.84 SCDD22 pKa = 4.17 LINSFVGSFDD32 pKa = 3.78 FNRR35 pKa = 11.84 NKK37 pKa = 10.77 SNTLQEE43 pKa = 4.59 LKK45 pKa = 10.51 EE46 pKa = 4.18 QTFHH50 pKa = 6.0 TKK52 pKa = 10.45 LYY54 pKa = 10.64 INIQNTLPFIKK65 pKa = 10.44 HH66 pKa = 6.53 NIPKK70 pKa = 9.91 AYY72 pKa = 10.34 CEE74 pKa = 3.87 LSYY77 pKa = 11.41 EE78 pKa = 4.72 NIVLWIQRR86 pKa = 11.84 ALYY89 pKa = 9.12 RR90 pKa = 11.84 INVATFIRR98 pKa = 11.84 FLEE101 pKa = 4.2 LDD103 pKa = 3.72 NIYY106 pKa = 10.99 NAGKK110 pKa = 10.28 FSLHH114 pKa = 6.29 HH115 pKa = 5.72 ICYY118 pKa = 10.19 EE119 pKa = 3.91 EE120 pKa = 4.01 EE121 pKa = 4.04 TRR123 pKa = 11.84 SNDD126 pKa = 3.07 VQAAFVDD133 pKa = 3.83 LRR135 pKa = 11.84 TRR137 pKa = 11.84 LLATLVADD145 pKa = 5.79 EE146 pKa = 5.38 IDD148 pKa = 3.4 TSSNEE153 pKa = 3.79 NTSIEE158 pKa = 4.13 MSPLMEE164 pKa = 4.13 RR165 pKa = 11.84 TLPLDD170 pKa = 3.84 GSAFGLYY177 pKa = 9.86 PEE179 pKa = 5.2 EE180 pKa = 5.92 DD181 pKa = 3.42 DD182 pKa = 6.48 DD183 pKa = 7.19 FILEE187 pKa = 4.16 LLKK190 pKa = 10.38 QRR192 pKa = 11.84 HH193 pKa = 5.1 EE194 pKa = 4.79 MITTLPDD201 pKa = 3.26 SDD203 pKa = 5.16 ALDD206 pKa = 4.08 ALVTADD212 pKa = 4.04 NLINVWKK219 pKa = 9.83 VAIIASMYY227 pKa = 10.52 RR228 pKa = 11.84 MPALEE233 pKa = 5.17 LPPGFPPNQSNDD245 pKa = 3.05 DD246 pKa = 3.92 VYY248 pKa = 11.13 IKK250 pKa = 10.67 QEE252 pKa = 3.96 DD253 pKa = 4.33 VLTDD257 pKa = 3.62 VYY259 pKa = 11.53 SDD261 pKa = 3.32 AQEE264 pKa = 3.98 YY265 pKa = 10.35 QYY267 pKa = 11.52 SQDD270 pKa = 3.04 RR271 pKa = 11.84 HH272 pKa = 5.75 LSQVYY277 pKa = 9.21 QLSQEE282 pKa = 4.31 GEE284 pKa = 4.3 SYY286 pKa = 10.99 EE287 pKa = 4.78 SNTHH291 pKa = 5.73 GNTPIKK297 pKa = 10.68 QEE299 pKa = 5.24 DD300 pKa = 3.67 DD301 pKa = 3.64 DD302 pKa = 4.79 NYY304 pKa = 11.5 NGIAAQQYY312 pKa = 9.93 GSTTFTTPCDD322 pKa = 3.6 SGDD325 pKa = 4.26 DD326 pKa = 4.01 SDD328 pKa = 5.32 HH329 pKa = 6.85 SYY331 pKa = 11.13 QGVEE335 pKa = 3.95 YY336 pKa = 10.59 LPNPVLATHH345 pKa = 7.41 ILPGDD350 pKa = 3.68 DD351 pKa = 3.72 PEE353 pKa = 4.6 FFEE356 pKa = 4.64 GDD358 pKa = 3.54 RR359 pKa = 11.84 YY360 pKa = 11.13 VLASPSLTEE369 pKa = 4.35 HH370 pKa = 7.35 DD371 pKa = 5.48 LPGDD375 pKa = 4.01 DD376 pKa = 6.02 DD377 pKa = 5.52 NDD379 pKa = 4.05 FEE381 pKa = 6.29 GDD383 pKa = 3.4 QYY385 pKa = 11.86 HH386 pKa = 7.21 LARR389 pKa = 11.84 PSFAEE394 pKa = 4.31 NGLPDD399 pKa = 4.13 NVEE402 pKa = 4.01 KK403 pKa = 10.85 DD404 pKa = 3.68 DD405 pKa = 5.12 EE406 pKa = 4.62 NGQSHH411 pKa = 7.34 LYY413 pKa = 9.76 EE414 pKa = 4.66 PPEE417 pKa = 4.23 DD418 pKa = 3.72 SNYY421 pKa = 10.57 CEE423 pKa = 4.74 DD424 pKa = 3.9 QLGTLDD430 pKa = 4.38 EE431 pKa = 4.49 QNKK434 pKa = 9.86 HH435 pKa = 6.47 PDD437 pKa = 3.34 TNINTDD443 pKa = 2.98 TDD445 pKa = 3.51 TDD447 pKa = 3.48 TDD449 pKa = 3.9 TNINITITINSDD461 pKa = 3.46 SITNSNTNTMNVHH474 pKa = 5.57 STDD477 pKa = 3.25 KK478 pKa = 11.34 AGDD481 pKa = 3.58 GSEE484 pKa = 4.06 GGGILEE490 pKa = 5.01 EE491 pKa = 4.65 NDD493 pKa = 3.86 DD494 pKa = 4.0 DD495 pKa = 5.9 AGNQLEE501 pKa = 4.49 EE502 pKa = 4.5 SEE504 pKa = 4.93 ADD506 pKa = 3.39 HH507 pKa = 7.28 DD508 pKa = 4.32 HH509 pKa = 6.77 GSVGTHH515 pKa = 5.44 TPIIDD520 pKa = 3.95 WNRR523 pKa = 11.84 HH524 pKa = 4.78 LAPSTAISDD533 pKa = 3.84 PFSDD537 pKa = 4.67 SEE539 pKa = 4.21 IGHH542 pKa = 6.72 DD543 pKa = 5.83 LEE545 pKa = 7.27 DD546 pKa = 4.16 NDD548 pKa = 5.66 DD549 pKa = 3.97 NNEE552 pKa = 3.71 FDD554 pKa = 4.98 LFPAAHH560 pKa = 6.97 EE561 pKa = 4.54 DD562 pKa = 4.56 DD563 pKa = 4.25 YY564 pKa = 12.16 AADD567 pKa = 3.7 VNNMYY572 pKa = 7.76 EE573 pKa = 4.4 TTSPWNFDD581 pKa = 3.47 DD582 pKa = 4.47 ATEE585 pKa = 4.06 FDD587 pKa = 3.96 INQQYY592 pKa = 9.1 TMVDD596 pKa = 4.24 EE597 pKa = 4.51 IAGDD601 pKa = 4.0 DD602 pKa = 5.2 DD603 pKa = 4.84 IDD605 pKa = 4.28 TPSEE609 pKa = 4.29 MPTKK613 pKa = 10.73 ADD615 pKa = 3.37 DD616 pKa = 3.62 TVTIDD621 pKa = 3.22 THH623 pKa = 4.74 TTAIDD628 pKa = 3.54 NSSSSQAHH636 pKa = 5.85 MDD638 pKa = 3.41 STSVHH643 pKa = 6.6 GISNQLEE650 pKa = 4.18 SSPVDD655 pKa = 3.04 THH657 pKa = 7.33 LATEE661 pKa = 4.31 AQNDD665 pKa = 3.9 DD666 pKa = 4.08 EE667 pKa = 5.12 GADD670 pKa = 3.81 DD671 pKa = 6.21 LVDD674 pKa = 3.97 EE675 pKa = 5.08 YY676 pKa = 11.33 EE677 pKa = 4.48 DD678 pKa = 3.14 VDD680 pKa = 3.87 NRR682 pKa = 11.84 VEE684 pKa = 3.97 ASAEE688 pKa = 3.81 ALEE691 pKa = 4.79 NDD693 pKa = 4.5 DD694 pKa = 4.99 LTTTSPTLAGAHH706 pKa = 5.15 VAGNDD711 pKa = 3.69 ASDD714 pKa = 3.72 SDD716 pKa = 4.01 EE717 pKa = 4.65 DD718 pKa = 5.97 LIDD721 pKa = 3.94 EE722 pKa = 5.41 LEE724 pKa = 4.49 NDD726 pKa = 4.46 DD727 pKa = 5.98 DD728 pKa = 6.63 DD729 pKa = 6.4 DD730 pKa = 6.01 SEE732 pKa = 4.59 YY733 pKa = 11.13 QLDD736 pKa = 4.32 PDD738 pKa = 4.35 EE739 pKa = 5.27 MDD741 pKa = 5.29 DD742 pKa = 4.93 SSSTNNSYY750 pKa = 10.71 IEE752 pKa = 4.2 TTGGEE757 pKa = 4.44 GEE759 pKa = 4.29 GRR761 pKa = 11.84 NN762 pKa = 3.71
Molecular weight: 84.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.722
IPC2_protein 3.706
IPC_protein 3.757
Toseland 3.528
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.668
Rodwell 3.579
Grimsley 3.427
Solomon 3.745
Lehninger 3.694
Nozaki 3.846
DTASelect 4.101
Thurlkill 3.567
EMBOSS 3.681
Sillero 3.872
Patrickios 1.863
IPC_peptide 3.732
IPC2_peptide 3.846
IPC2.peptide.svr19 3.785
Protein with the highest isoelectric point:
>tr|A0A1X2ILU6|A0A1X2ILU6_9FUNG Mitochondrial glycine transporter OS=Absidia repens OX=90262 GN=BCR42DRAFT_411493 PE=3 SV=1
MM1 pKa = 7.62 GNFTLGMLLVVMTMTRR17 pKa = 11.84 RR18 pKa = 11.84 LTMRR22 pKa = 11.84 FTSRR26 pKa = 11.84 LGRR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 GRR33 pKa = 11.84 DD34 pKa = 3.01 TTSIMGNFTLGMLLVIMTMTRR55 pKa = 11.84 RR56 pKa = 11.84 LTMRR60 pKa = 11.84 FTSRR64 pKa = 11.84 LGRR67 pKa = 11.84 RR68 pKa = 11.84 GRR70 pKa = 11.84 DD71 pKa = 3.09 TTSIMGNLTLSLATWFMVMGVFLLMMRR98 pKa = 11.84 VTADD102 pKa = 3.07 RR103 pKa = 11.84 RR104 pKa = 11.84 RR105 pKa = 11.84 RR106 pKa = 11.84 NTLLLMLGVTSGIHH120 pKa = 5.84 SSS122 pKa = 3.23
Molecular weight: 13.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.382
IPC2_protein 10.818
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.106
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.828
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.176
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14767
0
14767
6231592
49
7673
422.0
47.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.591 ± 0.018
1.387 ± 0.008
6.191 ± 0.017
5.196 ± 0.019
3.75 ± 0.014
5.371 ± 0.016
2.987 ± 0.012
5.486 ± 0.016
5.448 ± 0.02
9.168 ± 0.021
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.606 ± 0.008
4.753 ± 0.012
5.122 ± 0.022
5.284 ± 0.022
4.891 ± 0.013
8.81 ± 0.031
6.777 ± 0.017
5.693 ± 0.02
1.196 ± 0.007
3.292 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here