Prevotella sp. KH2C16
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2763 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I2PIP4|A0A1I2PIP4_9BACT Acyl-coenzyme A thioesterase PaaI contains HGG motif OS=Prevotella sp. KH2C16 OX=1855325 GN=SAMN05216383_10638 PE=4 SV=1
MM1 pKa = 7.31 SRR3 pKa = 11.84 EE4 pKa = 3.59 EE5 pKa = 4.35 EE6 pKa = 3.93 IRR8 pKa = 11.84 VTALEE13 pKa = 4.3 YY14 pKa = 10.62 VCRR17 pKa = 11.84 SYY19 pKa = 12.02 SNIDD23 pKa = 3.67 DD24 pKa = 4.02 AVEE27 pKa = 3.77 DD28 pKa = 4.16 AFVDD32 pKa = 4.2 GALWSDD38 pKa = 3.33 SHH40 pKa = 7.07 PANPWHH46 pKa = 6.44 SVADD50 pKa = 3.69 GDD52 pKa = 5.74 LPTEE56 pKa = 4.22 LKK58 pKa = 10.5 GDD60 pKa = 4.04 CVDD63 pKa = 3.44 MPFIVVCKK71 pKa = 10.76 DD72 pKa = 3.19 GITYY76 pKa = 9.91 SAYY79 pKa = 10.6 YY80 pKa = 10.2 DD81 pKa = 3.42 IEE83 pKa = 4.19 EE84 pKa = 4.9 DD85 pKa = 3.59 EE86 pKa = 4.63 EE87 pKa = 4.76 NYY89 pKa = 10.54 CFYY92 pKa = 11.17 DD93 pKa = 3.86 DD94 pKa = 4.85 CGCVLDD100 pKa = 3.61 VDD102 pKa = 4.16 YY103 pKa = 10.72 WLEE106 pKa = 4.04 IPQLPKK112 pKa = 10.53 EE113 pKa = 4.3 SEE115 pKa = 3.9 ARR117 pKa = 11.84 QQ118 pKa = 3.29
Molecular weight: 13.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.829
IPC2_protein 3.757
IPC_protein 3.745
Toseland 3.528
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.656
Rodwell 3.567
Grimsley 3.439
Solomon 3.719
Lehninger 3.668
Nozaki 3.846
DTASelect 4.062
Thurlkill 3.592
EMBOSS 3.668
Sillero 3.859
Patrickios 0.223
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.766
Protein with the highest isoelectric point:
>tr|A0A1I2Q5C4|A0A1I2Q5C4_9BACT Aspartate carbamoyltransferase regulatory chain OS=Prevotella sp. KH2C16 OX=1855325 GN=pyrI PE=3 SV=1
MM1 pKa = 7.85 PNGKK5 pKa = 9.19 KK6 pKa = 10.25 KK7 pKa = 10.12 KK8 pKa = 7.0 GHH10 pKa = 6.14 KK11 pKa = 9.06 MATHH15 pKa = 6.13 KK16 pKa = 10.39 RR17 pKa = 11.84 KK18 pKa = 9.84 KK19 pKa = 9.28 RR20 pKa = 11.84 LRR22 pKa = 11.84 KK23 pKa = 9.25 NRR25 pKa = 11.84 HH26 pKa = 4.69 KK27 pKa = 11.1 SKK29 pKa = 11.1
Molecular weight: 3.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.361
IPC2_protein 10.862
IPC_protein 12.325
Toseland 12.53
ProMoST 12.983
Dawson 12.544
Bjellqvist 12.501
Wikipedia 12.983
Rodwell 12.647
Grimsley 12.574
Solomon 12.998
Lehninger 12.91
Nozaki 12.53
DTASelect 12.501
Thurlkill 12.53
EMBOSS 13.013
Sillero 12.53
Patrickios 12.369
IPC_peptide 12.998
IPC2_peptide 11.974
IPC2.peptide.svr19 8.904
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2763
0
2763
959883
26
2518
347.4
39.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.517 ± 0.045
1.219 ± 0.015
5.794 ± 0.032
6.256 ± 0.045
4.406 ± 0.03
7.132 ± 0.033
2.064 ± 0.019
6.366 ± 0.044
6.273 ± 0.044
9.026 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.824 ± 0.022
4.818 ± 0.035
3.721 ± 0.026
3.562 ± 0.024
5.231 ± 0.043
6.071 ± 0.04
5.534 ± 0.035
6.434 ± 0.038
1.319 ± 0.02
4.431 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here