Amino acid dipepetide frequency for Prevotella sp. KH2C16

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.308AlaAla: 6.308 ± 0.11
1.057AlaCys: 1.057 ± 0.035
4.765AlaAsp: 4.765 ± 0.072
4.786AlaGlu: 4.786 ± 0.09
3.269AlaPhe: 3.269 ± 0.055
5.393AlaGly: 5.393 ± 0.096
1.387AlaHis: 1.387 ± 0.035
4.564AlaIle: 4.564 ± 0.084
4.419AlaLys: 4.419 ± 0.07
6.863AlaLeu: 6.863 ± 0.103
2.069AlaMet: 2.069 ± 0.048
3.242AlaAsn: 3.242 ± 0.071
2.393AlaPro: 2.393 ± 0.051
2.906AlaGln: 2.906 ± 0.06
3.902AlaArg: 3.902 ± 0.081
4.545AlaSer: 4.545 ± 0.077
4.055AlaThr: 4.055 ± 0.079
5.121AlaVal: 5.121 ± 0.09
0.919AlaTrp: 0.919 ± 0.031
3.209AlaTyr: 3.209 ± 0.057
0.0AlaXaa: 0.0 ± 0.0
Cys
0.794CysAla: 0.794 ± 0.03
0.19CysCys: 0.19 ± 0.014
0.674CysAsp: 0.674 ± 0.031
0.687CysGlu: 0.687 ± 0.029
0.556CysPhe: 0.556 ± 0.024
1.054CysGly: 1.054 ± 0.037
0.334CysHis: 0.334 ± 0.02
0.844CysIle: 0.844 ± 0.034
0.701CysLys: 0.701 ± 0.033
1.101CysLeu: 1.101 ± 0.036
0.358CysMet: 0.358 ± 0.02
0.551CysAsn: 0.551 ± 0.023
0.505CysPro: 0.505 ± 0.026
0.351CysGln: 0.351 ± 0.021
0.68CysArg: 0.68 ± 0.028
0.742CysSer: 0.742 ± 0.031
0.592CysThr: 0.592 ± 0.027
0.757CysVal: 0.757 ± 0.03
0.167CysTrp: 0.167 ± 0.014
0.554CysTyr: 0.554 ± 0.026
0.0CysXaa: 0.0 ± 0.0
Asp
4.408AspAla: 4.408 ± 0.077
0.69AspCys: 0.69 ± 0.028
3.127AspAsp: 3.127 ± 0.069
4.099AspGlu: 4.099 ± 0.082
3.204AspPhe: 3.204 ± 0.057
4.579AspGly: 4.579 ± 0.077
0.996AspHis: 0.996 ± 0.03
4.185AspIle: 4.185 ± 0.076
3.798AspLys: 3.798 ± 0.075
4.791AspLeu: 4.791 ± 0.082
1.66AspMet: 1.66 ± 0.041
2.777AspAsn: 2.777 ± 0.053
1.916AspPro: 1.916 ± 0.045
1.398AspGln: 1.398 ± 0.041
2.864AspArg: 2.864 ± 0.064
3.236AspSer: 3.236 ± 0.062
2.827AspThr: 2.827 ± 0.051
3.333AspVal: 3.333 ± 0.064
0.942AspTrp: 0.942 ± 0.03
3.11AspTyr: 3.11 ± 0.057
0.0AspXaa: 0.0 ± 0.0
Glu
5.165GluAla: 5.165 ± 0.08
0.638GluCys: 0.638 ± 0.027
3.439GluAsp: 3.439 ± 0.063
4.691GluGlu: 4.691 ± 0.101
2.421GluPhe: 2.421 ± 0.056
4.254GluGly: 4.254 ± 0.065
1.398GluHis: 1.398 ± 0.034
4.104GluIle: 4.104 ± 0.08
4.366GluLys: 4.366 ± 0.083
5.48GluLeu: 5.48 ± 0.085
1.918GluMet: 1.918 ± 0.052
3.176GluAsn: 3.176 ± 0.063
1.841GluPro: 1.841 ± 0.052
2.536GluGln: 2.536 ± 0.056
3.352GluArg: 3.352 ± 0.064
3.085GluSer: 3.085 ± 0.05
3.197GluThr: 3.197 ± 0.068
4.22GluVal: 4.22 ± 0.076
0.775GluTrp: 0.775 ± 0.029
2.509GluTyr: 2.509 ± 0.052
0.0GluXaa: 0.0 ± 0.0
Phe
3.155PheAla: 3.155 ± 0.059
0.707PheCys: 0.707 ± 0.025
2.789PheAsp: 2.789 ± 0.055
2.298PheGlu: 2.298 ± 0.05
2.018PhePhe: 2.018 ± 0.047
3.263PheGly: 3.263 ± 0.075
0.964PheHis: 0.964 ± 0.034
2.634PheIle: 2.634 ± 0.062
2.278PheLys: 2.278 ± 0.059
3.92PheLeu: 3.92 ± 0.078
1.249PheMet: 1.249 ± 0.034
2.211PheAsn: 2.211 ± 0.052
1.661PhePro: 1.661 ± 0.044
1.29PheGln: 1.29 ± 0.036
2.365PheArg: 2.365 ± 0.05
3.263PheSer: 3.263 ± 0.061
2.708PheThr: 2.708 ± 0.057
2.883PheVal: 2.883 ± 0.055
0.56PheTrp: 0.56 ± 0.02
1.85PheTyr: 1.85 ± 0.05
0.0PheXaa: 0.0 ± 0.0
Gly
4.803GlyAla: 4.803 ± 0.09
0.99GlyCys: 0.99 ± 0.037
3.845GlyAsp: 3.845 ± 0.067
4.204GlyGlu: 4.204 ± 0.071
3.293GlyPhe: 3.293 ± 0.062
5.193GlyGly: 5.193 ± 0.101
1.513GlyHis: 1.513 ± 0.048
5.1GlyIle: 5.1 ± 0.088
5.134GlyLys: 5.134 ± 0.07
6.052GlyLeu: 6.052 ± 0.099
2.146GlyMet: 2.146 ± 0.047
3.719GlyAsn: 3.719 ± 0.069
1.291GlyPro: 1.291 ± 0.042
2.386GlyGln: 2.386 ± 0.056
3.757GlyArg: 3.757 ± 0.071
4.393GlySer: 4.393 ± 0.075
4.281GlyThr: 4.281 ± 0.081
4.768GlyVal: 4.768 ± 0.072
1.091GlyTrp: 1.091 ± 0.035
3.362GlyTyr: 3.362 ± 0.057
0.0GlyXaa: 0.0 ± 0.0
His
1.403HisAla: 1.403 ± 0.045
0.299HisCys: 0.299 ± 0.017
1.187HisAsp: 1.187 ± 0.035
1.177HisGlu: 1.177 ± 0.033
1.055HisPhe: 1.055 ± 0.034
1.509HisGly: 1.509 ± 0.041
0.587HisHis: 0.587 ± 0.025
1.457HisIle: 1.457 ± 0.036
1.048HisLys: 1.048 ± 0.03
1.909HisLeu: 1.909 ± 0.048
0.442HisMet: 0.442 ± 0.022
0.946HisAsn: 0.946 ± 0.03
1.088HisPro: 1.088 ± 0.036
0.693HisGln: 0.693 ± 0.028
1.115HisArg: 1.115 ± 0.031
1.157HisSer: 1.157 ± 0.04
1.107HisThr: 1.107 ± 0.035
1.214HisVal: 1.214 ± 0.033
0.274HisTrp: 0.274 ± 0.016
0.975HisTyr: 0.975 ± 0.034
0.0HisXaa: 0.0 ± 0.0
Ile
5.058IleAla: 5.058 ± 0.082
0.842IleCys: 0.842 ± 0.031
4.33IleAsp: 4.33 ± 0.072
3.96IleGlu: 3.96 ± 0.066
2.468IlePhe: 2.468 ± 0.06
4.544IleGly: 4.544 ± 0.084
1.31IleHis: 1.31 ± 0.042
3.933IleIle: 3.933 ± 0.079
3.659IleLys: 3.659 ± 0.071
5.582IleLeu: 5.582 ± 0.105
1.472IleMet: 1.472 ± 0.041
3.035IleAsn: 3.035 ± 0.061
2.874IlePro: 2.874 ± 0.057
1.984IleGln: 1.984 ± 0.045
3.38IleArg: 3.38 ± 0.068
4.122IleSer: 4.122 ± 0.073
3.833IleThr: 3.833 ± 0.07
4.17IleVal: 4.17 ± 0.064
0.627IleTrp: 0.627 ± 0.025
2.483IleTyr: 2.483 ± 0.058
0.0IleXaa: 0.0 ± 0.0
Lys
5.35LysAla: 5.35 ± 0.083
0.495LysCys: 0.495 ± 0.026
3.77LysAsp: 3.77 ± 0.073
4.735LysGlu: 4.735 ± 0.092
2.186LysPhe: 2.186 ± 0.052
4.478LysGly: 4.478 ± 0.071
1.12LysHis: 1.12 ± 0.035
3.745LysIle: 3.745 ± 0.062
4.393LysLys: 4.393 ± 0.092
4.906LysLeu: 4.906 ± 0.074
2.021LysMet: 2.021 ± 0.038
3.2LysAsn: 3.2 ± 0.061
2.26LysPro: 2.26 ± 0.057
2.2LysGln: 2.2 ± 0.045
2.955LysArg: 2.955 ± 0.056
3.342LysSer: 3.342 ± 0.074
3.69LysThr: 3.69 ± 0.071
4.216LysVal: 4.216 ± 0.075
0.84LysTrp: 0.84 ± 0.031
2.826LysTyr: 2.826 ± 0.055
0.0LysXaa: 0.0 ± 0.0
Leu
6.659LeuAla: 6.659 ± 0.098
1.327LeuCys: 1.327 ± 0.043
4.887LeuAsp: 4.887 ± 0.077
4.789LeuGlu: 4.789 ± 0.083
4.033LeuPhe: 4.033 ± 0.075
6.021LeuGly: 6.021 ± 0.089
1.919LeuHis: 1.919 ± 0.045
4.986LeuIle: 4.986 ± 0.099
6.027LeuLys: 6.027 ± 0.083
8.707LeuLeu: 8.707 ± 0.163
2.627LeuMet: 2.627 ± 0.06
4.327LeuAsn: 4.327 ± 0.079
3.928LeuPro: 3.928 ± 0.066
3.265LeuGln: 3.265 ± 0.063
4.945LeuArg: 4.945 ± 0.099
6.448LeuSer: 6.448 ± 0.109
5.299LeuThr: 5.299 ± 0.081
5.327LeuVal: 5.327 ± 0.074
1.097LeuTrp: 1.097 ± 0.038
3.644LeuTyr: 3.644 ± 0.063
0.0LeuXaa: 0.0 ± 0.0
Met
2.344MetAla: 2.344 ± 0.053
0.242MetCys: 0.242 ± 0.017
1.588MetAsp: 1.588 ± 0.04
1.836MetGlu: 1.836 ± 0.045
1.003MetPhe: 1.003 ± 0.034
1.964MetGly: 1.964 ± 0.044
0.525MetHis: 0.525 ± 0.022
1.498MetIle: 1.498 ± 0.039
2.467MetLys: 2.467 ± 0.047
2.594MetLeu: 2.594 ± 0.054
0.859MetMet: 0.859 ± 0.034
1.459MetAsn: 1.459 ± 0.041
1.299MetPro: 1.299 ± 0.038
1.107MetGln: 1.107 ± 0.035
1.547MetArg: 1.547 ± 0.036
1.648MetSer: 1.648 ± 0.04
1.578MetThr: 1.578 ± 0.043
1.579MetVal: 1.579 ± 0.036
0.268MetTrp: 0.268 ± 0.02
0.832MetTyr: 0.832 ± 0.028
0.0MetXaa: 0.0 ± 0.0
Asn
3.645AsnAla: 3.645 ± 0.076
0.45AsnCys: 0.45 ± 0.022
2.618AsnAsp: 2.618 ± 0.063
2.709AsnGlu: 2.709 ± 0.062
2.162AsnPhe: 2.162 ± 0.046
3.797AsnGly: 3.797 ± 0.075
1.019AsnHis: 1.019 ± 0.034
3.574AsnIle: 3.574 ± 0.068
2.749AsnLys: 2.749 ± 0.056
4.388AsnLeu: 4.388 ± 0.075
1.222AsnMet: 1.222 ± 0.035
2.25AsnAsn: 2.25 ± 0.051
2.472AsnPro: 2.472 ± 0.046
1.631AsnGln: 1.631 ± 0.046
2.528AsnArg: 2.528 ± 0.056
2.715AsnSer: 2.715 ± 0.059
2.56AsnThr: 2.56 ± 0.058
2.713AsnVal: 2.713 ± 0.061
0.648AsnTrp: 0.648 ± 0.029
2.325AsnTyr: 2.325 ± 0.062
0.0AsnXaa: 0.0 ± 0.0
Pro
2.812ProAla: 2.812 ± 0.061
0.402ProCys: 0.402 ± 0.021
2.534ProAsp: 2.534 ± 0.059
3.087ProGlu: 3.087 ± 0.051
1.774ProPhe: 1.774 ± 0.038
2.435ProGly: 2.435 ± 0.054
0.719ProHis: 0.719 ± 0.028
2.13ProIle: 2.13 ± 0.046
2.027ProLys: 2.027 ± 0.05
3.229ProLeu: 3.229 ± 0.061
1.061ProMet: 1.061 ± 0.034
1.615ProAsn: 1.615 ± 0.036
0.802ProPro: 0.802 ± 0.034
1.492ProGln: 1.492 ± 0.046
1.643ProArg: 1.643 ± 0.038
2.289ProSer: 2.289 ± 0.054
2.069ProThr: 2.069 ± 0.048
2.875ProVal: 2.875 ± 0.061
0.474ProTrp: 0.474 ± 0.025
1.746ProTyr: 1.746 ± 0.044
0.0ProXaa: 0.0 ± 0.0
Gln
2.551GlnAla: 2.551 ± 0.054
0.294GlnCys: 0.294 ± 0.017
1.779GlnAsp: 1.779 ± 0.042
2.359GlnGlu: 2.359 ± 0.056
1.332GlnPhe: 1.332 ± 0.032
2.496GlnGly: 2.496 ± 0.05
0.665GlnHis: 0.665 ± 0.026
2.28GlnIle: 2.28 ± 0.051
2.473GlnLys: 2.473 ± 0.053
3.432GlnLeu: 3.432 ± 0.069
1.114GlnMet: 1.114 ± 0.031
1.738GlnAsn: 1.738 ± 0.045
1.355GlnPro: 1.355 ± 0.039
1.661GlnGln: 1.661 ± 0.051
1.87GlnArg: 1.87 ± 0.049
1.942GlnSer: 1.942 ± 0.047
2.034GlnThr: 2.034 ± 0.045
2.21GlnVal: 2.21 ± 0.05
0.508GlnTrp: 0.508 ± 0.022
1.53GlnTyr: 1.53 ± 0.044
0.0GlnXaa: 0.0 ± 0.0
Arg
3.088ArgAla: 3.088 ± 0.057
0.547ArgCys: 0.547 ± 0.024
2.599ArgAsp: 2.599 ± 0.058
3.469ArgGlu: 3.469 ± 0.072
2.345ArgPhe: 2.345 ± 0.053
2.94ArgGly: 2.94 ± 0.057
1.386ArgHis: 1.386 ± 0.038
3.684ArgIle: 3.684 ± 0.06
3.822ArgLys: 3.822 ± 0.065
5.117ArgLeu: 5.117 ± 0.076
1.752ArgMet: 1.752 ± 0.045
2.672ArgAsn: 2.672 ± 0.055
1.86ArgPro: 1.86 ± 0.046
2.573ArgGln: 2.573 ± 0.058
3.189ArgArg: 3.189 ± 0.07
2.603ArgSer: 2.603 ± 0.053
2.467ArgThr: 2.467 ± 0.051
2.836ArgVal: 2.836 ± 0.06
0.796ArgTrp: 0.796 ± 0.028
2.562ArgTyr: 2.562 ± 0.051
0.0ArgXaa: 0.0 ± 0.0
Ser
4.334SerAla: 4.334 ± 0.074
0.791SerCys: 0.791 ± 0.03
3.323SerAsp: 3.323 ± 0.055
3.476SerGlu: 3.476 ± 0.06
3.045SerPhe: 3.045 ± 0.069
4.436SerGly: 4.436 ± 0.077
1.275SerHis: 1.275 ± 0.04
3.958SerIle: 3.958 ± 0.065
3.237SerLys: 3.237 ± 0.058
6.102SerLeu: 6.102 ± 0.092
1.596SerMet: 1.596 ± 0.041
2.603SerAsn: 2.603 ± 0.058
2.271SerPro: 2.271 ± 0.051
2.129SerGln: 2.129 ± 0.044
3.081SerArg: 3.081 ± 0.06
3.859SerSer: 3.859 ± 0.081
3.185SerThr: 3.185 ± 0.066
4.222SerVal: 4.222 ± 0.07
0.924SerTrp: 0.924 ± 0.034
2.865SerTyr: 2.865 ± 0.068
0.0SerXaa: 0.0 ± 0.0
Thr
4.511ThrAla: 4.511 ± 0.063
0.568ThrCys: 0.568 ± 0.024
3.412ThrAsp: 3.412 ± 0.055
3.112ThrGlu: 3.112 ± 0.064
2.518ThrPhe: 2.518 ± 0.056
4.354ThrGly: 4.354 ± 0.073
1.096ThrHis: 1.096 ± 0.039
3.532ThrIle: 3.532 ± 0.063
2.732ThrLys: 2.732 ± 0.062
5.39ThrLeu: 5.39 ± 0.084
1.305ThrMet: 1.305 ± 0.033
2.269ThrAsn: 2.269 ± 0.059
2.725ThrPro: 2.725 ± 0.062
1.803ThrGln: 1.803 ± 0.045
2.54ThrArg: 2.54 ± 0.053
3.138ThrSer: 3.138 ± 0.064
3.37ThrThr: 3.37 ± 0.074
3.893ThrVal: 3.893 ± 0.07
0.676ThrTrp: 0.676 ± 0.028
2.396ThrTyr: 2.396 ± 0.054
0.0ThrXaa: 0.0 ± 0.0
Val
4.863ValAla: 4.863 ± 0.079
0.897ValCys: 0.897 ± 0.033
3.802ValAsp: 3.802 ± 0.062
3.91ValGlu: 3.91 ± 0.071
2.849ValPhe: 2.849 ± 0.054
4.374ValGly: 4.374 ± 0.074
1.108ValHis: 1.108 ± 0.036
4.009ValIle: 4.009 ± 0.069
4.002ValLys: 4.002 ± 0.06
5.607ValLeu: 5.607 ± 0.077
1.805ValMet: 1.805 ± 0.038
3.263ValAsn: 3.263 ± 0.066
2.544ValPro: 2.544 ± 0.045
1.919ValGln: 1.919 ± 0.049
3.404ValArg: 3.404 ± 0.069
4.626ValSer: 4.626 ± 0.071
3.09ValThr: 3.09 ± 0.06
4.761ValVal: 4.761 ± 0.081
0.772ValTrp: 0.772 ± 0.029
2.732ValTyr: 2.732 ± 0.058
0.0ValXaa: 0.0 ± 0.0
Trp
0.82TrpAla: 0.82 ± 0.034
0.177TrpCys: 0.177 ± 0.015
0.729TrpAsp: 0.729 ± 0.027
0.728TrpGlu: 0.728 ± 0.028
0.533TrpPhe: 0.533 ± 0.025
0.991TrpGly: 0.991 ± 0.033
0.331TrpHis: 0.331 ± 0.019
0.799TrpIle: 0.799 ± 0.031
0.901TrpLys: 0.901 ± 0.028
1.297TrpLeu: 1.297 ± 0.041
0.462TrpMet: 0.462 ± 0.022
0.838TrpAsn: 0.838 ± 0.029
0.308TrpPro: 0.308 ± 0.018
0.615TrpGln: 0.615 ± 0.023
0.68TrpArg: 0.68 ± 0.03
0.743TrpSer: 0.743 ± 0.03
0.776TrpThr: 0.776 ± 0.028
0.72TrpVal: 0.72 ± 0.029
0.218TrpTrp: 0.218 ± 0.015
0.528TrpTyr: 0.528 ± 0.027
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.102TyrAla: 3.102 ± 0.058
0.592TyrCys: 0.592 ± 0.024
2.742TyrAsp: 2.742 ± 0.06
2.453TyrGlu: 2.453 ± 0.053
1.999TyrPhe: 1.999 ± 0.047
3.245TyrGly: 3.245 ± 0.068
0.991TyrHis: 0.991 ± 0.032
2.648TyrIle: 2.648 ± 0.055
2.493TyrLys: 2.493 ± 0.058
3.794TyrLeu: 3.794 ± 0.068
1.099TyrMet: 1.099 ± 0.036
2.286TyrAsn: 2.286 ± 0.061
1.821TyrPro: 1.821 ± 0.047
1.685TyrGln: 1.685 ± 0.045
2.512TyrArg: 2.512 ± 0.047
2.817TyrSer: 2.817 ± 0.063
2.623TyrThr: 2.623 ± 0.059
2.517TyrVal: 2.517 ± 0.053
0.619TyrTrp: 0.619 ± 0.028
2.271TyrTyr: 2.271 ± 0.062
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2763 proteins (959883 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski