Meiothermus phage MMP7
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G8F4V1|A0A3G8F4V1_9CAUD Uncharacterized protein OS=Meiothermus phage MMP7 OX=2483850 PE=4 SV=1
MM1 pKa = 7.24 CQEE4 pKa = 4.39 CQGCGYY10 pKa = 10.11 VEE12 pKa = 4.32 YY13 pKa = 10.92 VAGFYY18 pKa = 10.83 FSDD21 pKa = 3.39 SFGNYY26 pKa = 9.42 LPRR29 pKa = 11.84 EE30 pKa = 4.13 EE31 pKa = 4.13 IARR34 pKa = 11.84 CEE36 pKa = 4.28 VCDD39 pKa = 3.43 GTGFVEE45 pKa = 5.14 VWDD48 pKa = 3.73 EE49 pKa = 4.3 DD50 pKa = 4.06 EE51 pKa = 5.73 AVLEE55 pKa = 4.42 GDD57 pKa = 3.85 GG58 pKa = 4.34
Molecular weight: 6.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.007
IPC2_protein 3.592
IPC_protein 3.439
Toseland 3.287
ProMoST 3.63
Dawson 3.439
Bjellqvist 3.605
Wikipedia 3.376
Rodwell 3.3
Grimsley 3.21
Solomon 3.376
Lehninger 3.325
Nozaki 3.592
DTASelect 3.668
Thurlkill 3.363
EMBOSS 3.389
Sillero 3.567
Patrickios 0.006
IPC_peptide 3.376
IPC2_peptide 3.528
IPC2.peptide.svr19 3.628
Protein with the highest isoelectric point:
>tr|A0A3G8F535|A0A3G8F535_9CAUD Major capsid protein OS=Meiothermus phage MMP7 OX=2483850 PE=4 SV=1
MM1 pKa = 7.53 TRR3 pKa = 11.84 LQWTFDD9 pKa = 3.5 LLDD12 pKa = 4.01 RR13 pKa = 11.84 VSAPARR19 pKa = 11.84 RR20 pKa = 11.84 IGQALKK26 pKa = 9.38 NTDD29 pKa = 3.25 AALKK33 pKa = 9.68 KK34 pKa = 10.22 VAGGGEE40 pKa = 4.05 AAQKK44 pKa = 10.56 ALQRR48 pKa = 11.84 SFGLSEE54 pKa = 3.76 RR55 pKa = 11.84 AAVRR59 pKa = 11.84 LTTAIATVGQVQGAIGGLQARR80 pKa = 11.84 VGALRR85 pKa = 11.84 GAFSSVTDD93 pKa = 3.6 SVFNLKK99 pKa = 10.01 NALVAGAIGFATKK112 pKa = 10.48 VVIDD116 pKa = 3.36 QVGFIEE122 pKa = 4.15 QQRR125 pKa = 11.84 IALGTILGSPIRR137 pKa = 11.84 ATAALDD143 pKa = 3.47 WAIKK147 pKa = 9.8 FADD150 pKa = 3.61 VTPFEE155 pKa = 4.44 TPQVLEE161 pKa = 4.31 AMRR164 pKa = 11.84 QALAVGFNTKK174 pKa = 10.08 QIEE177 pKa = 4.18 PLLTTLGDD185 pKa = 3.45 TAAALSLGPAGLNDD199 pKa = 5.62 LITVFGQIRR208 pKa = 11.84 SAGKK212 pKa = 10.54 LNTEE216 pKa = 4.37 EE217 pKa = 4.4 VNQLTEE223 pKa = 3.94 RR224 pKa = 11.84 GIPAFEE230 pKa = 4.06 ILAKK234 pKa = 10.57 AFSTDD239 pKa = 2.85 IPTVRR244 pKa = 11.84 QMIEE248 pKa = 3.48 KK249 pKa = 10.12 GQIASEE255 pKa = 4.05 AALGAIYY262 pKa = 10.33 RR263 pKa = 11.84 GLKK266 pKa = 8.28 TRR268 pKa = 11.84 FGGGMAAQSRR278 pKa = 11.84 SIFGLVSTLRR288 pKa = 11.84 SRR290 pKa = 11.84 PQTIAFRR297 pKa = 11.84 LEE299 pKa = 3.49 RR300 pKa = 11.84 DD301 pKa = 3.25 KK302 pKa = 11.51 ALEE305 pKa = 3.81 PFRR308 pKa = 11.84 RR309 pKa = 11.84 LLMNLADD316 pKa = 3.75 LTDD319 pKa = 4.1 FNKK322 pKa = 10.55 PPGSTIAARR331 pKa = 11.84 LTSGIGGLFKK341 pKa = 10.8 AAFGPLAAATEE352 pKa = 4.2 PKK354 pKa = 10.24 RR355 pKa = 11.84 AGEE358 pKa = 3.89 AVLAFINRR366 pKa = 11.84 ATAAVNRR373 pKa = 11.84 ARR375 pKa = 11.84 DD376 pKa = 3.17 AWPAVKK382 pKa = 9.96 AAALDD387 pKa = 4.42 FISGVRR393 pKa = 11.84 SGFDD397 pKa = 3.25 LLAGIWRR404 pKa = 11.84 TVEE407 pKa = 3.88 PLVSGLGRR415 pKa = 11.84 LAGSFTGAQASMGGANINAVKK436 pKa = 10.44 IIGTIAALAAVWRR449 pKa = 11.84 VLNLVTLGGAGAIARR464 pKa = 11.84 WGAIAVVSLVRR475 pKa = 11.84 VAAVGLPILWAKK487 pKa = 10.41 IGALNTLGVTALRR500 pKa = 11.84 ASGRR504 pKa = 11.84 ALLAGGRR511 pKa = 11.84 MALAWIIGLGPIGWLIGGVAAISGALVLAYY541 pKa = 10.78 NKK543 pKa = 9.87 VDD545 pKa = 3.09 WFRR548 pKa = 11.84 NVVNRR553 pKa = 11.84 AWEE556 pKa = 4.29 SIKK559 pKa = 10.59 QVGKK563 pKa = 10.95 GLLDD567 pKa = 3.5 WFTSLPTRR575 pKa = 11.84 IGQTFAQLPNLLRR588 pKa = 11.84 GLLRR592 pKa = 11.84 RR593 pKa = 11.84 AIDD596 pKa = 3.82 LLPPGVRR603 pKa = 11.84 DD604 pKa = 3.65 VVRR607 pKa = 11.84 GLVGGLLDD615 pKa = 4.8 GSDD618 pKa = 3.78 PVEE621 pKa = 4.22 NAATQLADD629 pKa = 3.22 KK630 pKa = 8.07 TQQGFARR637 pKa = 11.84 PLEE640 pKa = 4.0 IRR642 pKa = 11.84 SPSRR646 pKa = 11.84 RR647 pKa = 11.84 FAYY650 pKa = 9.48 YY651 pKa = 10.07 GQMMGAGLEE660 pKa = 4.0 VGMRR664 pKa = 11.84 DD665 pKa = 2.93 SLGRR669 pKa = 11.84 VQRR672 pKa = 11.84 AAAGMTIAATLAMSGGPALSVAPPALAPTTRR703 pKa = 11.84 AISITVGPITVNGGSDD719 pKa = 3.24 TKK721 pKa = 11.0 QIADD725 pKa = 3.92 EE726 pKa = 4.25 LRR728 pKa = 11.84 TVAVEE733 pKa = 4.15 AVLEE737 pKa = 4.13 ALEE740 pKa = 4.32 RR741 pKa = 11.84 AAHH744 pKa = 6.06 EE745 pKa = 4.44 EE746 pKa = 4.36 GAA748 pKa = 3.95
Molecular weight: 78.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.317
IPC2_protein 9.56
IPC_protein 10.409
Toseland 10.921
ProMoST 10.73
Dawson 10.965
Bjellqvist 10.701
Wikipedia 11.213
Rodwell 11.082
Grimsley 10.994
Solomon 11.169
Lehninger 11.125
Nozaki 10.891
DTASelect 10.701
Thurlkill 10.891
EMBOSS 11.345
Sillero 10.906
Patrickios 10.804
IPC_peptide 11.184
IPC2_peptide 9.458
IPC2.peptide.svr19 8.949
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
53
0
53
10268
40
748
193.7
21.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.096 ± 0.514
0.779 ± 0.167
4.704 ± 0.213
7.372 ± 0.586
2.376 ± 0.201
8.239 ± 0.408
1.529 ± 0.223
4.1 ± 0.265
2.649 ± 0.276
11.102 ± 0.37
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.558 ± 0.095
2.678 ± 0.224
6.048 ± 0.347
3.759 ± 0.192
8.668 ± 0.432
4.48 ± 0.159
5.619 ± 0.37
6.515 ± 0.311
2.707 ± 0.208
3.019 ± 0.246
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here