Blastococcus sp. CCUG 61487

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Geodermatophilales; Geodermatophilaceae; Blastococcus; unclassified Blastococcus

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3688 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V5TZJ7|A0A4V5TZJ7_9ACTN Pilus assembly protein CpaF OS=Blastococcus sp. CCUG 61487 OX=1840703 GN=A6V29_08360 PE=3 SV=1
MM1 pKa = 7.6AGAGGRR7 pKa = 11.84RR8 pKa = 11.84AALLPAVLACLLVLTGCSMVVVGQAIPALPPVTDD42 pKa = 4.23AEE44 pKa = 4.36PGEE47 pKa = 4.22IEE49 pKa = 4.6VVGATDD55 pKa = 3.71QEE57 pKa = 4.4VDD59 pKa = 3.52VLARR63 pKa = 11.84NALADD68 pKa = 4.85LEE70 pKa = 4.8TFWAEE75 pKa = 3.48QQPAVFGLPFEE86 pKa = 4.53PLEE89 pKa = 4.32GGYY92 pKa = 10.63FSVDD96 pKa = 3.21PGDD99 pKa = 3.48VDD101 pKa = 3.45PRR103 pKa = 11.84LYY105 pKa = 9.23PTGIGCGADD114 pKa = 3.3PRR116 pKa = 11.84EE117 pKa = 4.28VEE119 pKa = 4.31DD120 pKa = 3.43NAYY123 pKa = 10.42YY124 pKa = 10.75CLARR128 pKa = 11.84EE129 pKa = 4.72APNSDD134 pKa = 3.81AISYY138 pKa = 10.59DD139 pKa = 3.25RR140 pKa = 11.84TFLGDD145 pKa = 3.81LGADD149 pKa = 4.19FGRR152 pKa = 11.84FIPALVMAHH161 pKa = 6.2EE162 pKa = 5.31FGHH165 pKa = 6.69AVQARR170 pKa = 11.84VGSPEE175 pKa = 3.63FSILVEE181 pKa = 4.2TQADD185 pKa = 4.05CLAGAWSRR193 pKa = 11.84WVADD197 pKa = 3.71GAAEE201 pKa = 4.1HH202 pKa = 5.91TVLRR206 pKa = 11.84TSEE209 pKa = 4.27LDD211 pKa = 3.47DD212 pKa = 3.59VLRR215 pKa = 11.84GYY217 pKa = 11.18LLLRR221 pKa = 11.84DD222 pKa = 3.96PVGTNPGQPQAHH234 pKa = 6.42GSYY237 pKa = 10.16FDD239 pKa = 3.68RR240 pKa = 11.84VSAFQEE246 pKa = 4.44GFEE249 pKa = 4.82LGAEE253 pKa = 4.16ACRR256 pKa = 11.84DD257 pKa = 3.69NFDD260 pKa = 3.19VARR263 pKa = 11.84PLTQDD268 pKa = 3.06RR269 pKa = 11.84FRR271 pKa = 11.84TVGEE275 pKa = 4.03IEE277 pKa = 4.2TGGNAPYY284 pKa = 10.46EE285 pKa = 4.23VLLDD289 pKa = 3.79IAAEE293 pKa = 4.1TLPQAWASALGDD305 pKa = 3.77DD306 pKa = 4.6FRR308 pKa = 11.84PPSVEE313 pKa = 3.96RR314 pKa = 11.84FDD316 pKa = 5.84GEE318 pKa = 4.93APDD321 pKa = 4.52CAPDD325 pKa = 4.19PGLDD329 pKa = 4.34LVYY332 pKa = 10.8CPDD335 pKa = 4.81DD336 pKa = 4.0GLVAFDD342 pKa = 4.11EE343 pKa = 4.56TDD345 pKa = 3.48LAVPAYY351 pKa = 10.13EE352 pKa = 4.11EE353 pKa = 4.66LGDD356 pKa = 3.88FAVATAISLPYY367 pKa = 9.96GLAVRR372 pKa = 11.84DD373 pKa = 3.79QLGLSVDD380 pKa = 4.27DD381 pKa = 4.04EE382 pKa = 4.45DD383 pKa = 3.99AARR386 pKa = 11.84SVVCLSGWYY395 pKa = 10.02AEE397 pKa = 4.3QLYY400 pKa = 10.37SDD402 pKa = 4.61PLGADD407 pKa = 4.44GIFTSSISPGDD418 pKa = 3.26VDD420 pKa = 4.71EE421 pKa = 5.08SVQFLLTFGSEE432 pKa = 4.33PEE434 pKa = 4.14VLPGVDD440 pKa = 3.24LTGFQLVDD448 pKa = 4.03LYY450 pKa = 11.14RR451 pKa = 11.84AGFFEE456 pKa = 5.45GLDD459 pKa = 3.5ACGLL463 pKa = 3.81

Molecular weight: 49.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V5TZR4|A0A4V5TZR4_9ACTN Hydrolase OS=Blastococcus sp. CCUG 61487 OX=1840703 GN=A6V29_07305 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 8.98RR4 pKa = 11.84TFQPNTRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84SKK15 pKa = 8.65THH17 pKa = 5.48GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAGRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 10.08GRR40 pKa = 11.84EE41 pKa = 3.61KK42 pKa = 10.93LSAA45 pKa = 3.78

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3688

0

3688

1142701

29

2637

309.8

33.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.992 ± 0.064

0.686 ± 0.011

6.392 ± 0.03

5.92 ± 0.033

2.587 ± 0.024

9.372 ± 0.041

2.014 ± 0.017

3.055 ± 0.029

1.519 ± 0.029

10.69 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.667 ± 0.015

1.496 ± 0.02

6.086 ± 0.033

2.764 ± 0.022

8.214 ± 0.043

4.836 ± 0.029

5.882 ± 0.033

9.526 ± 0.041

1.511 ± 0.017

1.791 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski