Lyngbya confervoides BDU141951
Average proteome isoelectric point is 5.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6060 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C1UPF6|A0A0C1UPF6_9CYAN DNA gyrase subunit B OS=Lyngbya confervoides BDU141951 OX=1574623 GN=gyrB PE=3 SV=1
MM1 pKa = 7.44 KK2 pKa = 10.53 LFVGLLSAGLVLAGGLSFASPSYY25 pKa = 9.33 GQAVVATPRR34 pKa = 11.84 SPALDD39 pKa = 3.28 IQSTQIQYY47 pKa = 11.02 LEE49 pKa = 4.14 EE50 pKa = 5.65 LDD52 pKa = 4.52 LLVFEE57 pKa = 4.46 QQLAGVVGATLPQSAGQLDD76 pKa = 4.16 GAPVLGYY83 pKa = 9.39 VFPTTLSPAAVGFGGVDD100 pKa = 4.43 GILALAVTSHH110 pKa = 7.56 PDD112 pKa = 3.75 FDD114 pKa = 5.28 DD115 pKa = 3.96 TPLWDD120 pKa = 4.15 EE121 pKa = 5.84 DD122 pKa = 3.74 NDD124 pKa = 3.86 ADD126 pKa = 4.1 YY127 pKa = 11.77 ANDD130 pKa = 3.53 GVIWHH135 pKa = 5.8 THH137 pKa = 3.78 WVVLTGDD144 pKa = 3.31 NRR146 pKa = 11.84 VPGGLSVAEE155 pKa = 4.6 FAADD159 pKa = 3.65 DD160 pKa = 4.32 ASVVMPPTNPGMTIYY175 pKa = 10.56 LDD177 pKa = 3.61 SPGFSVVTDD186 pKa = 3.48 QEE188 pKa = 4.47 TLRR191 pKa = 11.84 VLVPAQRR198 pKa = 11.84 VDD200 pKa = 3.07 HH201 pKa = 6.0 NTDD204 pKa = 3.51 FNFDD208 pKa = 3.13 AVTAYY213 pKa = 9.25 MQVNTSDD220 pKa = 3.33 ASRR223 pKa = 11.84 PTLGVYY229 pKa = 8.76 EE230 pKa = 4.49 VYY232 pKa = 10.71 SVGSGDD238 pKa = 3.62 LSLPYY243 pKa = 10.07 GVRR246 pKa = 11.84 HH247 pKa = 6.69 DD248 pKa = 4.37 ADD250 pKa = 3.27
Molecular weight: 26.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.68
IPC2_protein 3.757
IPC_protein 3.783
Toseland 3.541
ProMoST 3.923
Dawson 3.795
Bjellqvist 3.973
Wikipedia 3.757
Rodwell 3.605
Grimsley 3.452
Solomon 3.783
Lehninger 3.745
Nozaki 3.91
DTASelect 4.202
Thurlkill 3.617
EMBOSS 3.77
Sillero 3.91
Patrickios 1.1
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.818
Protein with the highest isoelectric point:
>tr|A0A0C1USQ4|A0A0C1USQ4_9CYAN Multicopper oxidase OS=Lyngbya confervoides BDU141951 OX=1574623 GN=QQ91_30110 PE=4 SV=1
MM1 pKa = 7.38 TEE3 pKa = 3.43 ITRR6 pKa = 11.84 IQAALRR12 pKa = 11.84 PHH14 pKa = 6.92 LSWHH18 pKa = 5.47 GARR21 pKa = 11.84 LTFLALFLVALFRR34 pKa = 11.84 VEE36 pKa = 4.29 TVNLEE41 pKa = 3.84 RR42 pKa = 11.84 LASVFANRR50 pKa = 11.84 AQSASNHH57 pKa = 4.98 KK58 pKa = 10.17 RR59 pKa = 11.84 LTRR62 pKa = 11.84 FFRR65 pKa = 11.84 KK66 pKa = 9.45 FVIDD70 pKa = 3.87 FEE72 pKa = 5.11 EE73 pKa = 4.21 IAQAVVSWSQIPEE86 pKa = 3.93 PWTLSLDD93 pKa = 3.51 RR94 pKa = 11.84 TNWSFGTVNFNILMLGIVHH113 pKa = 6.89 EE114 pKa = 5.01 GVAFPVMWTMLDD126 pKa = 3.09 KK127 pKa = 11.06 RR128 pKa = 11.84 GNSNSDD134 pKa = 2.72 EE135 pKa = 4.45 RR136 pKa = 11.84 MDD138 pKa = 3.79 LLEE141 pKa = 4.09 RR142 pKa = 11.84 FEE144 pKa = 5.07 RR145 pKa = 11.84 VFPNAQIRR153 pKa = 11.84 CLTGDD158 pKa = 3.43 RR159 pKa = 11.84 EE160 pKa = 4.39 FVGRR164 pKa = 11.84 EE165 pKa = 3.49 WCSFLLVPDD174 pKa = 5.78 AIPFRR179 pKa = 11.84 LRR181 pKa = 11.84 LRR183 pKa = 11.84 HH184 pKa = 5.91 SDD186 pKa = 3.76 RR187 pKa = 11.84 ISSRR191 pKa = 11.84 SGKK194 pKa = 8.75 QRR196 pKa = 11.84 QTGEE200 pKa = 3.96 RR201 pKa = 11.84 VFANLKK207 pKa = 10.06 VGEE210 pKa = 3.98 QRR212 pKa = 11.84 HH213 pKa = 5.39 LSGKK217 pKa = 7.9 RR218 pKa = 11.84 WVWGRR223 pKa = 11.84 RR224 pKa = 11.84 VYY226 pKa = 10.38 IVATRR231 pKa = 11.84 LEE233 pKa = 4.54 DD234 pKa = 4.14 GEE236 pKa = 4.72 LLILATGHH244 pKa = 5.67 QPQTAFADD252 pKa = 3.6 YY253 pKa = 10.14 RR254 pKa = 11.84 LRR256 pKa = 11.84 WGIEE260 pKa = 3.82 TLFATLKK267 pKa = 9.6 TRR269 pKa = 11.84 GFNLEE274 pKa = 4.08 STHH277 pKa = 5.89 FCHH280 pKa = 7.72 AEE282 pKa = 3.88 RR283 pKa = 11.84 LSKK286 pKa = 10.85 LIALLALAFCWAMLTGLWQHH306 pKa = 5.51 QQHH309 pKa = 7.42 PIPLKK314 pKa = 9.02 AHH316 pKa = 5.36 GRR318 pKa = 11.84 RR319 pKa = 11.84 AKK321 pKa = 10.36 SLFRR325 pKa = 11.84 YY326 pKa = 9.9 GCDD329 pKa = 3.22 FLRR332 pKa = 11.84 RR333 pKa = 11.84 TFSDD337 pKa = 4.52 FSLRR341 pKa = 11.84 RR342 pKa = 11.84 SEE344 pKa = 4.73 FNQALKK350 pKa = 10.85 LLSLYY355 pKa = 10.79
Molecular weight: 41.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.487
IPC_protein 10.409
Toseland 10.73
ProMoST 10.54
Dawson 10.804
Bjellqvist 10.555
Wikipedia 11.052
Rodwell 10.862
Grimsley 10.833
Solomon 10.994
Lehninger 10.95
Nozaki 10.73
DTASelect 10.54
Thurlkill 10.73
EMBOSS 11.155
Sillero 10.745
Patrickios 10.57
IPC_peptide 10.994
IPC2_peptide 9.765
IPC2.peptide.svr19 8.729
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6060
0
6060
1735983
28
10778
286.5
31.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.311 ± 0.041
0.945 ± 0.012
5.768 ± 0.04
6.014 ± 0.036
3.869 ± 0.023
7.49 ± 0.046
2.021 ± 0.02
5.563 ± 0.028
3.134 ± 0.032
10.717 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.211 ± 0.018
3.199 ± 0.031
5.179 ± 0.035
4.912 ± 0.03
5.806 ± 0.037
5.611 ± 0.027
5.886 ± 0.038
7.086 ± 0.024
1.502 ± 0.014
2.772 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here