Pedobacter cryoconitis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Pedobacter

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4911 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A127V9X9|A0A127V9X9_9SPHI AsnC family transcriptional regulator OS=Pedobacter cryoconitis OX=188932 GN=AY601_1281 PE=4 SV=1
MM1 pKa = 7.85SNFQVDD7 pKa = 4.18LTNCDD12 pKa = 3.37IEE14 pKa = 5.0PIHH17 pKa = 7.68IPGQIQSHH25 pKa = 6.0GFLVVIDD32 pKa = 5.2DD33 pKa = 3.67EE34 pKa = 4.72MSIQFHH40 pKa = 6.92SDD42 pKa = 3.12NINNFIAPVPDD53 pKa = 3.45VLIGKK58 pKa = 9.44SIGHH62 pKa = 6.45IEE64 pKa = 4.12SLLDD68 pKa = 3.29QDD70 pKa = 4.44YY71 pKa = 11.27QPDD74 pKa = 3.55QAGSS78 pKa = 3.23

Molecular weight: 8.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A127VEE5|A0A127VEE5_9SPHI RNA polymerase ECF-type sigma factor OS=Pedobacter cryoconitis OX=188932 GN=AY601_2793 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.54RR3 pKa = 11.84TFQPSQRR10 pKa = 11.84KK11 pKa = 8.72RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.3HH16 pKa = 3.94GFRR19 pKa = 11.84EE20 pKa = 4.19RR21 pKa = 11.84MATANGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.19GRR39 pKa = 11.84RR40 pKa = 11.84KK41 pKa = 10.24LSVSDD46 pKa = 3.67EE47 pKa = 4.18RR48 pKa = 11.84RR49 pKa = 11.84HH50 pKa = 5.73KK51 pKa = 10.89AA52 pKa = 3.07

Molecular weight: 6.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4911

0

4911

1755082

50

11234

357.4

40.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.347 ± 0.034

0.772 ± 0.011

5.235 ± 0.025

5.826 ± 0.042

4.925 ± 0.031

6.686 ± 0.041

1.857 ± 0.016

7.585 ± 0.032

7.236 ± 0.042

9.816 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.248 ± 0.016

5.629 ± 0.038

3.704 ± 0.02

3.824 ± 0.024

3.685 ± 0.021

6.477 ± 0.028

5.753 ± 0.044

6.202 ± 0.028

1.082 ± 0.012

4.111 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski