Absidia glauca (Pin mould)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Mucoromycota; Mucoromycotina; Mucoromycetes; Mucorales; Cunninghamellaceae; Absidia

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14825 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A163JZ98|A0A163JZ98_ABSGL Uncharacterized protein OS=Absidia glauca OX=4829 GN=ABSGL_11451.1 scaffold 12295 PE=4 SV=1
MM1 pKa = 7.77PALTLDD7 pKa = 3.74RR8 pKa = 11.84LQAAVCFLKK17 pKa = 10.47MSLDD21 pKa = 3.54DD22 pKa = 3.91HH23 pKa = 6.4GVRR26 pKa = 11.84FAPPLSAQALEE37 pKa = 4.26KK38 pKa = 10.65FEE40 pKa = 5.1CEE42 pKa = 3.79HH43 pKa = 5.71TVRR46 pKa = 11.84LPEE49 pKa = 4.53DD50 pKa = 3.26YY51 pKa = 10.93RR52 pKa = 11.84FFLTLIGNGGQSGPCFEE69 pKa = 4.87GVLPLGVFPFTSNPEE84 pKa = 3.96SLLNNLPDD92 pKa = 4.39LFPFTEE98 pKa = 4.2PSILIGGGGGQQQDD112 pKa = 3.91SDD114 pKa = 5.39DD115 pKa = 5.55DD116 pKa = 4.09DD117 pKa = 4.69TDD119 pKa = 4.35LCDD122 pKa = 3.68TTSDD126 pKa = 4.15DD127 pKa = 4.56NGASTDD133 pKa = 3.78DD134 pKa = 4.0SLDD137 pKa = 4.22GILGNTYY144 pKa = 10.56SSSDD148 pKa = 3.27NDD150 pKa = 4.61DD151 pKa = 4.74RR152 pKa = 11.84EE153 pKa = 4.42DD154 pKa = 5.56DD155 pKa = 3.85YY156 pKa = 12.1DD157 pKa = 6.39DD158 pKa = 5.94YY159 pKa = 12.09DD160 pKa = 6.76DD161 pKa = 6.84DD162 pKa = 7.48DD163 pKa = 7.23DD164 pKa = 7.7DD165 pKa = 5.4DD166 pKa = 4.82DD167 pKa = 6.75KK168 pKa = 12.26YY169 pKa = 8.86MTSYY173 pKa = 11.45SLDD176 pKa = 3.41EE177 pKa = 4.07EE178 pKa = 5.42LYY180 pKa = 10.16PNLQVVEE187 pKa = 4.21QQQQQQRR194 pKa = 11.84DD195 pKa = 3.63GSIKK199 pKa = 10.83HH200 pKa = 6.4NDD202 pKa = 2.92TMTVGGYY209 pKa = 10.61LILGTLAQDD218 pKa = 3.57RR219 pKa = 11.84QYY221 pKa = 11.45FWILICGGACRR232 pKa = 11.84GEE234 pKa = 3.7VWIMNKK240 pKa = 10.0HH241 pKa = 5.25GFYY244 pKa = 10.54FPTSRR249 pKa = 11.84RR250 pKa = 11.84LKK252 pKa = 8.97FYY254 pKa = 10.57EE255 pKa = 4.2WVEE258 pKa = 3.91DD259 pKa = 3.58WLLGGDD265 pKa = 4.99KK266 pKa = 10.55INASLWACGLYY277 pKa = 10.52DD278 pKa = 3.94PQFSRR283 pKa = 11.84CFQSGIDD290 pKa = 3.7NATSDD295 pKa = 3.62SGSDD299 pKa = 3.4QEE301 pKa = 4.29EE302 pKa = 4.96DD303 pKa = 3.72YY304 pKa = 11.77GNSSDD309 pKa = 3.93NDD311 pKa = 4.04SNSGYY316 pKa = 10.82SYY318 pKa = 11.39DD319 pKa = 5.36DD320 pKa = 3.51EE321 pKa = 4.6LL322 pKa = 6.09

Molecular weight:
35.97 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A168RTF4|A0A168RTF4_ABSGL DNA polymerase epsilon subunit OS=Absidia glauca OX=4829 GN=ABSGL_13010.1 scaffold 13554 PE=3 SV=1
MM1 pKa = 7.95DD2 pKa = 4.8FSSHH6 pKa = 6.5RR7 pKa = 11.84EE8 pKa = 4.16SIGKK12 pKa = 7.4TFSSPGIRR20 pKa = 11.84SNKK23 pKa = 9.18KK24 pKa = 8.4IHH26 pKa = 6.43INCGSSARR34 pKa = 11.84MAGNMCANVDD44 pKa = 3.62QIRR47 pKa = 11.84RR48 pKa = 11.84QGRR51 pKa = 11.84RR52 pKa = 11.84DD53 pKa = 3.1NTTINGAYY61 pKa = 7.77LTNLPRR67 pKa = 11.84EE68 pKa = 4.67LVRR71 pKa = 11.84SMAGSLTNGRR81 pKa = 11.84FFYY84 pKa = 10.72LARR87 pKa = 11.84AALNPCGIARR97 pKa = 11.84EE98 pKa = 4.21EE99 pKa = 4.05

Molecular weight:
10.94 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14825

0

14825

6549974

19

6879

441.8

49.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.916 ± 0.018

1.377 ± 0.008

6.274 ± 0.016

5.082 ± 0.017

3.652 ± 0.012

5.412 ± 0.018

3.053 ± 0.012

5.141 ± 0.013

5.315 ± 0.017

9.318 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.538 ± 0.007

4.323 ± 0.011

5.635 ± 0.023

5.151 ± 0.021

5.163 ± 0.014

8.663 ± 0.027

6.779 ± 0.016

5.751 ± 0.017

1.213 ± 0.006

3.182 ± 0.01

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski