Absidia glauca (Pin mould)
Average proteome isoelectric point is 6.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14825 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A163JZ98|A0A163JZ98_ABSGL Uncharacterized protein OS=Absidia glauca OX=4829 GN=ABSGL_11451.1 scaffold 12295 PE=4 SV=1
MM1 pKa = 7.77 PALTLDD7 pKa = 3.74 RR8 pKa = 11.84 LQAAVCFLKK17 pKa = 10.47 MSLDD21 pKa = 3.54 DD22 pKa = 3.91 HH23 pKa = 6.4 GVRR26 pKa = 11.84 FAPPLSAQALEE37 pKa = 4.26 KK38 pKa = 10.65 FEE40 pKa = 5.1 CEE42 pKa = 3.79 HH43 pKa = 5.71 TVRR46 pKa = 11.84 LPEE49 pKa = 4.53 DD50 pKa = 3.26 YY51 pKa = 10.93 RR52 pKa = 11.84 FFLTLIGNGGQSGPCFEE69 pKa = 4.87 GVLPLGVFPFTSNPEE84 pKa = 3.96 SLLNNLPDD92 pKa = 4.39 LFPFTEE98 pKa = 4.2 PSILIGGGGGQQQDD112 pKa = 3.91 SDD114 pKa = 5.39 DD115 pKa = 5.55 DD116 pKa = 4.09 DD117 pKa = 4.69 TDD119 pKa = 4.35 LCDD122 pKa = 3.68 TTSDD126 pKa = 4.15 DD127 pKa = 4.56 NGASTDD133 pKa = 3.78 DD134 pKa = 4.0 SLDD137 pKa = 4.22 GILGNTYY144 pKa = 10.56 SSSDD148 pKa = 3.27 NDD150 pKa = 4.61 DD151 pKa = 4.74 RR152 pKa = 11.84 EE153 pKa = 4.42 DD154 pKa = 5.56 DD155 pKa = 3.85 YY156 pKa = 12.1 DD157 pKa = 6.39 DD158 pKa = 5.94 YY159 pKa = 12.09 DD160 pKa = 6.76 DD161 pKa = 6.84 DD162 pKa = 7.48 DD163 pKa = 7.23 DD164 pKa = 7.7 DD165 pKa = 5.4 DD166 pKa = 4.82 DD167 pKa = 6.75 KK168 pKa = 12.26 YY169 pKa = 8.86 MTSYY173 pKa = 11.45 SLDD176 pKa = 3.41 EE177 pKa = 4.07 EE178 pKa = 5.42 LYY180 pKa = 10.16 PNLQVVEE187 pKa = 4.21 QQQQQQRR194 pKa = 11.84 DD195 pKa = 3.63 GSIKK199 pKa = 10.83 HH200 pKa = 6.4 NDD202 pKa = 2.92 TMTVGGYY209 pKa = 10.61 LILGTLAQDD218 pKa = 3.57 RR219 pKa = 11.84 QYY221 pKa = 11.45 FWILICGGACRR232 pKa = 11.84 GEE234 pKa = 3.7 VWIMNKK240 pKa = 10.0 HH241 pKa = 5.25 GFYY244 pKa = 10.54 FPTSRR249 pKa = 11.84 RR250 pKa = 11.84 LKK252 pKa = 8.97 FYY254 pKa = 10.57 EE255 pKa = 4.2 WVEE258 pKa = 3.91 DD259 pKa = 3.58 WLLGGDD265 pKa = 4.99 KK266 pKa = 10.55 INASLWACGLYY277 pKa = 10.52 DD278 pKa = 3.94 PQFSRR283 pKa = 11.84 CFQSGIDD290 pKa = 3.7 NATSDD295 pKa = 3.62 SGSDD299 pKa = 3.4 QEE301 pKa = 4.29 EE302 pKa = 4.96 DD303 pKa = 3.72 YY304 pKa = 11.77 GNSSDD309 pKa = 3.93 NDD311 pKa = 4.04 SNSGYY316 pKa = 10.82 SYY318 pKa = 11.39 DD319 pKa = 5.36 DD320 pKa = 3.51 EE321 pKa = 4.6 LL322 pKa = 6.09
Molecular weight: 35.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.707
IPC2_protein 3.668
IPC_protein 3.719
Toseland 3.478
ProMoST 3.884
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.681
Rodwell 3.528
Grimsley 3.376
Solomon 3.719
Lehninger 3.668
Nozaki 3.821
DTASelect 4.126
Thurlkill 3.528
EMBOSS 3.681
Sillero 3.834
Patrickios 0.757
IPC_peptide 3.706
IPC2_peptide 3.808
IPC2.peptide.svr19 3.755
Protein with the highest isoelectric point:
>tr|A0A168RTF4|A0A168RTF4_ABSGL DNA polymerase epsilon subunit OS=Absidia glauca OX=4829 GN=ABSGL_13010.1 scaffold 13554 PE=3 SV=1
MM1 pKa = 7.95 DD2 pKa = 4.8 FSSHH6 pKa = 6.5 RR7 pKa = 11.84 EE8 pKa = 4.16 SIGKK12 pKa = 7.4 TFSSPGIRR20 pKa = 11.84 SNKK23 pKa = 9.18 KK24 pKa = 8.4 IHH26 pKa = 6.43 INCGSSARR34 pKa = 11.84 MAGNMCANVDD44 pKa = 3.62 QIRR47 pKa = 11.84 RR48 pKa = 11.84 QGRR51 pKa = 11.84 RR52 pKa = 11.84 DD53 pKa = 3.1 NTTINGAYY61 pKa = 7.77 LTNLPRR67 pKa = 11.84 EE68 pKa = 4.67 LVRR71 pKa = 11.84 SMAGSLTNGRR81 pKa = 11.84 FFYY84 pKa = 10.72 LARR87 pKa = 11.84 AALNPCGIARR97 pKa = 11.84 EE98 pKa = 4.21 EE99 pKa = 4.05
Molecular weight: 10.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 9.545
IPC_protein 10.496
Toseland 10.599
ProMoST 10.379
Dawson 10.701
Bjellqvist 10.482
Wikipedia 10.95
Rodwell 10.774
Grimsley 10.76
Solomon 10.862
Lehninger 10.818
Nozaki 10.628
DTASelect 10.452
Thurlkill 10.613
EMBOSS 11.008
Sillero 10.657
Patrickios 10.57
IPC_peptide 10.862
IPC2_peptide 9.853
IPC2.peptide.svr19 8.511
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14825
0
14825
6549974
19
6879
441.8
49.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.916 ± 0.018
1.377 ± 0.008
6.274 ± 0.016
5.082 ± 0.017
3.652 ± 0.012
5.412 ± 0.018
3.053 ± 0.012
5.141 ± 0.013
5.315 ± 0.017
9.318 ± 0.022
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.538 ± 0.007
4.323 ± 0.011
5.635 ± 0.023
5.151 ± 0.021
5.163 ± 0.014
8.663 ± 0.027
6.779 ± 0.016
5.751 ± 0.017
1.213 ± 0.006
3.182 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here