Kitasatospora sp. OK780

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Kitasatospora; unclassified Kitasatospora

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7543 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N0JID6|A0A2N0JID6_9ACTN Uncharacterized protein OS=Kitasatospora sp. OK780 OX=1882771 GN=BDB15_0092 PE=4 SV=1
MM1 pKa = 7.81PLFTAVVFDD10 pKa = 5.08GIDD13 pKa = 3.31WDD15 pKa = 4.09GDD17 pKa = 3.63AALHH21 pKa = 5.9RR22 pKa = 11.84FVIEE26 pKa = 4.49APDD29 pKa = 3.36EE30 pKa = 3.96EE31 pKa = 4.49AARR34 pKa = 11.84LVVYY38 pKa = 10.31ALQGCEE44 pKa = 3.9EE45 pKa = 4.4VKK47 pKa = 10.88SSVVEE52 pKa = 4.15EE53 pKa = 3.92AWQIYY58 pKa = 10.16ALVPAEE64 pKa = 3.88PCGPEE69 pKa = 3.85TYY71 pKa = 10.46DD72 pKa = 3.56CLRR75 pKa = 11.84VTGPGTWEE83 pKa = 3.92WTDD86 pKa = 3.21QPTPP90 pKa = 3.51

Molecular weight: 9.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N0JT47|A0A2N0JT47_9ACTN Acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II OS=Kitasatospora sp. OK780 OX=1882771 GN=BDB15_3646 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.51GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7543

0

7543

2465880

29

4931

326.9

34.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.741 ± 0.048

0.8 ± 0.008

5.83 ± 0.019

5.135 ± 0.031

2.614 ± 0.015

9.74 ± 0.031

2.322 ± 0.015

2.95 ± 0.018

1.714 ± 0.018

10.62 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.579 ± 0.01

1.705 ± 0.017

6.447 ± 0.031

2.837 ± 0.018

8.099 ± 0.035

4.84 ± 0.024

6.141 ± 0.028

8.369 ± 0.029

1.534 ± 0.011

1.983 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski