Idotea virus IWaV278
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H4YQ33|A0A2H4YQ33_9VIRU Putative capsid protein OS=Idotea virus IWaV278 OX=2058759 PE=3 SV=1
MM1 pKa = 7.8 PNGSKK6 pKa = 10.39 RR7 pKa = 11.84 FCFTHH12 pKa = 6.69 FGCPDD17 pKa = 3.45 EE18 pKa = 4.47 FTNILQARR26 pKa = 11.84 SPLCTKK32 pKa = 10.02 WIVQQEE38 pKa = 4.05 RR39 pKa = 11.84 GQAEE43 pKa = 4.18 GKK45 pKa = 10.69 APLEE49 pKa = 4.28 GANAQNGGLHH59 pKa = 5.58 LQGYY63 pKa = 8.22 IHH65 pKa = 6.8 TKK67 pKa = 9.58 RR68 pKa = 11.84 VYY70 pKa = 10.13 TFAQFLRR77 pKa = 11.84 IWPVSHH83 pKa = 7.09 VEE85 pKa = 3.79 AAQGTEE91 pKa = 3.73 AQNYY95 pKa = 7.44 LYY97 pKa = 10.39 CRR99 pKa = 11.84 KK100 pKa = 10.2 NEE102 pKa = 4.13 TFTGKK107 pKa = 10.14 YY108 pKa = 8.15 RR109 pKa = 11.84 LAMEE113 pKa = 4.65 GSVWSRR119 pKa = 11.84 DD120 pKa = 3.75 VQRR123 pKa = 11.84 LQAKK127 pKa = 8.34 SRR129 pKa = 11.84 KK130 pKa = 8.39 DD131 pKa = 3.46 AEE133 pKa = 4.06 MQAIVDD139 pKa = 4.45 KK140 pKa = 10.39 IKK142 pKa = 10.88 QGATTADD149 pKa = 3.02 IARR152 pKa = 11.84 NNTAYY157 pKa = 10.58 FIRR160 pKa = 11.84 HH161 pKa = 6.12 CNGIPAAVRR170 pKa = 11.84 HH171 pKa = 5.81 HH172 pKa = 6.3 QPAAPATRR180 pKa = 11.84 PVHH183 pKa = 6.42 VILLKK188 pKa = 10.93 GSTGTGKK195 pKa = 10.6 SYY197 pKa = 9.83 WARR200 pKa = 11.84 QLATYY205 pKa = 7.99 QRR207 pKa = 11.84 KK208 pKa = 8.81 RR209 pKa = 11.84 LYY211 pKa = 10.36 CKK213 pKa = 10.32 QIQQDD218 pKa = 3.54 TDD220 pKa = 3.83 TQWFDD225 pKa = 3.66 GYY227 pKa = 11.02 DD228 pKa = 3.53 GQEE231 pKa = 3.8 ILLLDD236 pKa = 3.92 DD237 pKa = 5.19 FGRR240 pKa = 11.84 KK241 pKa = 8.7 QIGYY245 pKa = 8.59 RR246 pKa = 11.84 QLLTYY251 pKa = 10.66 LDD253 pKa = 3.85 IYY255 pKa = 10.18 ALKK258 pKa = 10.56 VQIKK262 pKa = 10.16 GDD264 pKa = 3.69 TVDD267 pKa = 4.28 ARR269 pKa = 11.84 WNYY272 pKa = 10.22 VIITTNEE279 pKa = 4.31 EE280 pKa = 3.64 IQEE283 pKa = 4.14 WYY285 pKa = 9.46 PNEE288 pKa = 5.25 DD289 pKa = 2.83 IAPLRR294 pKa = 11.84 RR295 pKa = 11.84 RR296 pKa = 11.84 IAKK299 pKa = 9.03 IHH301 pKa = 5.52 EE302 pKa = 4.68 CNRR305 pKa = 11.84 PWAVDD310 pKa = 3.36 YY311 pKa = 11.76 VDD313 pKa = 5.28 FKK315 pKa = 11.41 RR316 pKa = 11.84 PLPVPVVPQFVLPPEE331 pKa = 4.5 PEE333 pKa = 3.88 GKK335 pKa = 10.35 YY336 pKa = 9.37 PQPEE340 pKa = 4.3 PNQQAHH346 pKa = 6.63 AVPIVDD352 pKa = 3.37 EE353 pKa = 4.45 VEE355 pKa = 4.36 YY356 pKa = 10.83 EE357 pKa = 4.06 DD358 pKa = 4.72 LVPVEE363 pKa = 5.59 DD364 pKa = 4.99 LDD366 pKa = 5.33 ADD368 pKa = 4.01 ALIDD372 pKa = 4.63 EE373 pKa = 5.05 INAEE377 pKa = 4.25 LGAIEE382 pKa = 5.21 DD383 pKa = 4.11 PPDD386 pKa = 3.93 FEE388 pKa = 4.65 MADD391 pKa = 3.53 LGHH394 pKa = 6.66 LVWRR398 pKa = 11.84 PMM400 pKa = 3.63
Molecular weight: 45.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.73
IPC2_protein 5.715
IPC_protein 5.766
Toseland 6.148
ProMoST 6.097
Dawson 6.02
Bjellqvist 6.033
Wikipedia 6.033
Rodwell 6.008
Grimsley 6.287
Solomon 6.02
Lehninger 6.008
Nozaki 6.287
DTASelect 6.478
Thurlkill 6.478
EMBOSS 6.44
Sillero 6.389
Patrickios 4.05
IPC_peptide 6.033
IPC2_peptide 6.376
IPC2.peptide.svr19 6.34
Protein with the highest isoelectric point:
>tr|A0A2H4YQ33|A0A2H4YQ33_9VIRU Putative capsid protein OS=Idotea virus IWaV278 OX=2058759 PE=3 SV=1
MM1 pKa = 7.12 SKK3 pKa = 10.12 KK4 pKa = 10.29 RR5 pKa = 11.84 KK6 pKa = 7.03 TVTGGGSRR14 pKa = 11.84 RR15 pKa = 11.84 STGVPYY21 pKa = 9.86 WQMKK25 pKa = 9.4 AARR28 pKa = 11.84 QYY30 pKa = 11.0 RR31 pKa = 11.84 DD32 pKa = 3.97 DD33 pKa = 4.67 YY34 pKa = 9.34 MAEE37 pKa = 4.07 YY38 pKa = 9.5 PRR40 pKa = 11.84 GSAASKK46 pKa = 11.05 AMWGDD51 pKa = 3.87 DD52 pKa = 3.37 YY53 pKa = 11.82 KK54 pKa = 11.23 SASKK58 pKa = 9.95 RR59 pKa = 11.84 QRR61 pKa = 11.84 YY62 pKa = 8.14 LRR64 pKa = 11.84 NAMRR68 pKa = 11.84 FKK70 pKa = 11.33 GKK72 pKa = 10.28 GKK74 pKa = 10.48 YY75 pKa = 8.02 SAKK78 pKa = 10.54 NFGRR82 pKa = 11.84 DD83 pKa = 3.0 ALRR86 pKa = 11.84 YY87 pKa = 9.76 GGAALGGGIGYY98 pKa = 7.44 MTGGLAGAGAMAQRR112 pKa = 11.84 GYY114 pKa = 9.8 DD115 pKa = 3.22 TGADD119 pKa = 3.35 LSRR122 pKa = 11.84 FVGFGEE128 pKa = 4.16 YY129 pKa = 9.94 KK130 pKa = 10.55 GANQLIAGPEE140 pKa = 3.9 QNMSITVNRR149 pKa = 11.84 EE150 pKa = 3.44 HH151 pKa = 7.58 RR152 pKa = 11.84 SGDD155 pKa = 3.01 IVFANTEE162 pKa = 4.11 FVQNVYY168 pKa = 10.97 AGVTSGPSTSKK179 pKa = 10.55 FQTQAFSLNPGLGEE193 pKa = 4.06 TFPFLSQIAQNFEE206 pKa = 4.02 LFEE209 pKa = 4.27 FEE211 pKa = 5.18 GLMFEE216 pKa = 4.55 YY217 pKa = 10.41 KK218 pKa = 8.85 PTSGEE223 pKa = 4.01 FGTTSSNSLGKK234 pKa = 10.44 VVMCTNYY241 pKa = 10.59 DD242 pKa = 3.39 PEE244 pKa = 5.68 APEE247 pKa = 4.19 FQNSVQMEE255 pKa = 4.32 NYY257 pKa = 10.33 DD258 pKa = 3.73 YY259 pKa = 11.84 ANSTKK264 pKa = 9.92 PSRR267 pKa = 11.84 GAIHH271 pKa = 6.63 GVEE274 pKa = 3.97 THH276 pKa = 6.87 PDD278 pKa = 3.01 QRR280 pKa = 11.84 ATKK283 pKa = 10.14 LMYY286 pKa = 10.62 VRR288 pKa = 11.84 TGDD291 pKa = 3.57 ANKK294 pKa = 10.75 SKK296 pKa = 10.86 LFTDD300 pKa = 4.46 LGTLHH305 pKa = 7.55 LATEE309 pKa = 5.05 GVPFGGDD316 pKa = 3.31 GQQKK320 pKa = 10.75 ALIGEE325 pKa = 4.29 LWVTYY330 pKa = 9.81 KK331 pKa = 10.57 VRR333 pKa = 11.84 LSRR336 pKa = 11.84 SQLYY340 pKa = 10.83 SSVLGNNVITSYY352 pKa = 11.28 QSGTSDD358 pKa = 3.15 SGVWTGTKK366 pKa = 10.04 QFDD369 pKa = 4.03 SNTWDD374 pKa = 3.05 MSLTNVSQGVFKK386 pKa = 9.86 FTLPEE391 pKa = 4.27 DD392 pKa = 3.84 VDD394 pKa = 4.29 DD395 pKa = 5.25 GSFLVTISNSSTGTHH410 pKa = 5.17 TMSPLEE416 pKa = 4.04 FTLEE420 pKa = 3.83 NCKK423 pKa = 8.69 FTKK426 pKa = 10.0 TPVAIAPPPSQVQGWNRR443 pKa = 11.84 TYY445 pKa = 11.36 LMKK448 pKa = 10.45 VDD450 pKa = 3.99 AQSALNICSFQFDD463 pKa = 4.71 DD464 pKa = 5.77 PDD466 pKa = 4.75 SNWPNGSWQVGITQVSDD483 pKa = 3.0 KK484 pKa = 11.28 LYY486 pKa = 10.83 DD487 pKa = 3.1 EE488 pKa = 4.48 WAAFSGEE495 pKa = 4.04 LPAA498 pKa = 6.55
Molecular weight: 54.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.713
IPC2_protein 6.942
IPC_protein 7.146
Toseland 7.029
ProMoST 7.541
Dawson 7.761
Bjellqvist 7.629
Wikipedia 7.717
Rodwell 7.746
Grimsley 7.102
Solomon 7.892
Lehninger 7.892
Nozaki 7.819
DTASelect 7.819
Thurlkill 7.863
EMBOSS 8.009
Sillero 8.068
Patrickios 4.215
IPC_peptide 7.892
IPC2_peptide 7.029
IPC2.peptide.svr19 7.416
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
898
400
498
449.0
50.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.129 ± 0.536
1.114 ± 0.392
5.791 ± 0.437
5.345 ± 0.711
4.454 ± 0.742
8.686 ± 1.654
1.782 ± 0.596
4.12 ± 1.311
5.234 ± 0.144
6.682 ± 0.35
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.227 ± 0.602
4.454 ± 0.434
5.345 ± 0.865
6.125 ± 0.693
5.345 ± 0.711
6.125 ± 2.54
6.682 ± 0.881
5.902 ± 0.214
2.004 ± 0.151
4.454 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here