Bosea thiooxidans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Boseaceae; Bosea

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4956 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q3HZ22|A0A0Q3HZ22_9BRAD Aminopeptidase N OS=Bosea thiooxidans OX=53254 GN=pepN PE=3 SV=1
MM1 pKa = 8.09PDD3 pKa = 2.98LTGYY7 pKa = 11.0DD8 pKa = 3.47LVLASTEE15 pKa = 4.1SALNSSIAYY24 pKa = 9.82ANFWKK29 pKa = 9.07VTINCQLNPDD39 pKa = 3.77VSAEE43 pKa = 3.95QSPGLVGARR52 pKa = 11.84ISAPTLRR59 pKa = 11.84LSGSDD64 pKa = 3.27LASNQAAVSLLFTAGSLQYY83 pKa = 10.65FDD85 pKa = 4.29VIYY88 pKa = 10.84YY89 pKa = 7.88RR90 pKa = 11.84TVSQNIAGWTIVFAANIGQGAIPDD114 pKa = 3.74NTEE117 pKa = 3.49IGQLPAEE124 pKa = 4.4VQNALSNQRR133 pKa = 11.84LSVGQLFLEE142 pKa = 4.88LSTVVWANVTITDD155 pKa = 3.72ADD157 pKa = 3.92GKK159 pKa = 10.48RR160 pKa = 11.84VTDD163 pKa = 5.09AIITSTLEE171 pKa = 3.61TALQSWARR179 pKa = 11.84SIEE182 pKa = 4.14SAAQQWVLNYY192 pKa = 10.07VVTSSGGPGNASGEE206 pKa = 4.31VLADD210 pKa = 4.16LVPTSYY216 pKa = 11.27DD217 pKa = 3.42FSVSKK222 pKa = 10.47YY223 pKa = 9.74NPPGTYY229 pKa = 10.3DD230 pKa = 4.53DD231 pKa = 5.82LSTLNYY237 pKa = 11.12LMMTEE242 pKa = 4.35GRR244 pKa = 11.84GAPTANSAGIFDD256 pKa = 5.02FNWVAGPNTQQYY268 pKa = 9.5QGAVAINGPLFDD280 pKa = 3.92GAYY283 pKa = 10.04VEE285 pKa = 4.77PLLLPAIAAAVSATKK300 pKa = 8.73PTSAGGSTGNPGVPTFTRR318 pKa = 11.84TGPGQWSTSQSVDD331 pKa = 3.32LYY333 pKa = 11.16GNQHH337 pKa = 5.82GGKK340 pKa = 8.57GQKK343 pKa = 10.31LEE345 pKa = 4.09GLGLLQAVYY354 pKa = 10.99QNITEE359 pKa = 4.42KK360 pKa = 10.88LDD362 pKa = 3.54CTVTLDD368 pKa = 4.02PASATYY374 pKa = 8.94QVKK377 pKa = 8.09VTYY380 pKa = 10.37SAITTITVEE389 pKa = 3.76FFGWDD394 pKa = 3.14KK395 pKa = 10.94TDD397 pKa = 3.51SSFSGDD403 pKa = 3.89YY404 pKa = 10.17VLPWSFDD411 pKa = 3.41VRR413 pKa = 11.84LKK415 pKa = 10.91ASSNGSLVTEE425 pKa = 4.12STEE428 pKa = 4.05VQEE431 pKa = 5.15GEE433 pKa = 4.51SSSKK437 pKa = 8.38TWGNSFVGDD446 pKa = 3.14IWATISEE453 pKa = 4.51VFSSFLNTYY462 pKa = 7.41QTQFQDD468 pKa = 3.1KK469 pKa = 9.2AASVSQSAFANVSDD483 pKa = 4.14EE484 pKa = 4.77LEE486 pKa = 4.12SAMGDD491 pKa = 2.93ISAAIVFPAGNRR503 pKa = 11.84FAYY506 pKa = 9.8KK507 pKa = 10.06DD508 pKa = 3.46VCFDD512 pKa = 3.56PQQNLVAYY520 pKa = 7.46VTYY523 pKa = 11.04LNN525 pKa = 4.08

Molecular weight: 55.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q3M325|A0A0Q3M325_9BRAD DNA-binding transcriptional regulator GntR family OS=Bosea thiooxidans OX=53254 GN=ARD30_14750 PE=4 SV=1
MM1 pKa = 7.6SARR4 pKa = 11.84LPSLLLLVALLAGWEE19 pKa = 4.12AWCRR23 pKa = 11.84LGGVSALVLPPPSAVLATLWSEE45 pKa = 3.94IATGRR50 pKa = 11.84LWPHH54 pKa = 6.58LAVTATEE61 pKa = 4.15MALGLAIGAVVGLGVGILLAEE82 pKa = 4.88FPALSRR88 pKa = 11.84LLRR91 pKa = 11.84PYY93 pKa = 10.69VLASQLVPKK102 pKa = 10.21LALGPLFIIWFGFGMTPTVVITALICFFPLMEE134 pKa = 4.27NTLTGVSEE142 pKa = 3.98VDD144 pKa = 3.21PARR147 pKa = 11.84RR148 pKa = 11.84EE149 pKa = 4.02LFRR152 pKa = 11.84MLGASRR158 pKa = 11.84LQTLLRR164 pKa = 11.84LKK166 pKa = 10.63LPAALPVIMAGLRR179 pKa = 11.84VAVVLALVGAVVGEE193 pKa = 4.46FIGGRR198 pKa = 11.84IGLGASIIAAQSVMDD213 pKa = 4.03SSLIFALFIVITALGMLLYY232 pKa = 10.34EE233 pKa = 4.4AVRR236 pKa = 11.84LLEE239 pKa = 3.82RR240 pKa = 11.84RR241 pKa = 11.84VLHH244 pKa = 6.35RR245 pKa = 11.84FSKK248 pKa = 10.8GG249 pKa = 3.0

Molecular weight: 26.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4956

0

4956

1551163

29

1793

313.0

33.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.603 ± 0.044

0.797 ± 0.009

5.23 ± 0.028

5.593 ± 0.03

3.708 ± 0.021

8.903 ± 0.035

1.918 ± 0.017

5.108 ± 0.024

3.16 ± 0.029

10.641 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.364 ± 0.015

2.286 ± 0.017

5.39 ± 0.027

3.013 ± 0.021

7.335 ± 0.036

5.099 ± 0.022

5.003 ± 0.021

7.446 ± 0.028

1.328 ± 0.013

2.074 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski