Amino acid dipepetide frequency for Plasmodium falciparum IGH-CR14

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	0.93AlaAla: 0.93 ± 0.03	0.479AlaCys: 0.479 ± 0.016	1.092AlaAsp: 1.092 ± 0.024	1.231AlaGlu: 1.231 ± 0.03	0.902AlaPhe: 0.902 ± 0.018	0.737AlaGly: 0.737 ± 0.019	0.586AlaHis: 0.586 ± 0.021	1.586AlaIle: 1.586 ± 0.028	1.744AlaLys: 1.744 ± 0.039	1.845AlaLeu: 1.845 ± 0.025	0.356AlaMet: 0.356 ± 0.012	1.585AlaAsn: 1.585 ± 0.026	0.592AlaPro: 0.592 ± 0.019	0.699AlaGln: 0.699 ± 0.015	0.584AlaArg: 0.584 ± 0.015	1.489AlaSer: 1.489 ± 0.03	0.984AlaThr: 0.984 ± 0.025	0.865AlaVal: 0.865 ± 0.021	0.137AlaTrp: 0.137 ± 0.007	1.063AlaTyr: 1.063 ± 0.02	0.0AlaXaa: 0.0 ± 0.0
Cys	0.519CysAla: 0.519 ± 0.014	0.32CysCys: 0.32 ± 0.011	1.351CysAsp: 1.351 ± 0.03	1.137CysGlu: 1.137 ± 0.024	0.808CysPhe: 0.808 ± 0.017	0.717CysGly: 0.717 ± 0.018	0.327CysHis: 0.327 ± 0.011	1.714CysIle: 1.714 ± 0.022	1.624CysLys: 1.624 ± 0.031	1.505CysLeu: 1.505 ± 0.023	0.36CysMet: 0.36 ± 0.012	1.883CysAsn: 1.883 ± 0.029	0.464CysPro: 0.464 ± 0.016	0.344CysGln: 0.344 ± 0.01	0.488CysArg: 0.488 ± 0.02	1.397CysSer: 1.397 ± 0.026	0.944CysThr: 0.944 ± 0.019	0.891CysVal: 0.891 ± 0.018	0.063CysTrp: 0.063 ± 0.004	0.821CysTyr: 0.821 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	1.236AspAla: 1.236 ± 0.023	0.701AspCys: 0.701 ± 0.022	7.249AspAsp: 7.249 ± 0.129	5.941AspGlu: 5.941 ± 0.071	2.098AspPhe: 2.098 ± 0.027	1.805AspGly: 1.805 ± 0.033	1.531AspHis: 1.531 ± 0.028	7.409AspIle: 7.409 ± 0.06	6.833AspLys: 6.833 ± 0.074	3.734AspLeu: 3.734 ± 0.039	1.741AspMet: 1.741 ± 0.027	9.242AspAsn: 9.242 ± 0.105	1.03AspPro: 1.03 ± 0.023	1.461AspGln: 1.461 ± 0.026	1.237AspArg: 1.237 ± 0.031	3.162AspSer: 3.162 ± 0.042	2.764AspThr: 2.764 ± 0.036	3.003AspVal: 3.003 ± 0.048	0.223AspTrp: 0.223 ± 0.01	2.858AspTyr: 2.858 ± 0.035	0.0AspXaa: 0.0 ± 0.0
Glu	1.413GluAla: 1.413 ± 0.03	1.101GluCys: 1.101 ± 0.022	4.776GluAsp: 4.776 ± 0.062	8.754GluGlu: 8.754 ± 0.461	1.969GluPhe: 1.969 ± 0.03	2.098GluGly: 2.098 ± 0.047	1.872GluHis: 1.872 ± 0.032	5.376GluIle: 5.376 ± 0.084	10.932GluLys: 10.932 ± 0.095	4.583GluLeu: 4.583 ± 0.111	1.402GluMet: 1.402 ± 0.028	9.064GluAsn: 9.064 ± 0.088	1.021GluPro: 1.021 ± 0.026	2.741GluGln: 2.741 ± 0.051	2.17GluArg: 2.17 ± 0.052	3.302GluSer: 3.302 ± 0.044	2.406GluThr: 2.406 ± 0.029	2.4GluVal: 2.4 ± 0.289	0.501GluTrp: 0.501 ± 0.021	3.713GluTyr: 3.713 ± 0.034	0.0GluXaa: 0.0 ± 0.0
Phe	0.785PheAla: 0.785 ± 0.017	0.949PheCys: 0.949 ± 0.019	2.426PheAsp: 2.426 ± 0.032	2.305PheGlu: 2.305 ± 0.025	3.237PhePhe: 3.237 ± 0.052	1.142PheGly: 1.142 ± 0.025	1.129PheHis: 1.129 ± 0.021	4.19PheIle: 4.19 ± 0.055	3.603PheLys: 3.603 ± 0.04	4.914PheLeu: 4.914 ± 0.06	0.889PheMet: 0.889 ± 0.018	4.253PheAsn: 4.253 ± 0.047	0.979PhePro: 0.979 ± 0.015	1.095PheGln: 1.095 ± 0.018	0.951PheArg: 0.951 ± 0.016	3.195PheSer: 3.195 ± 0.042	1.546PheThr: 1.546 ± 0.021	1.943PheVal: 1.943 ± 0.027	0.2PheTrp: 0.2 ± 0.008	2.798PheTyr: 2.798 ± 0.04	0.0PheXaa: 0.0 ± 0.0
Gly	0.828GlyAla: 0.828 ± 0.025	0.528GlyCys: 0.528 ± 0.016	2.253GlyAsp: 2.253 ± 0.048	1.913GlyGlu: 1.913 ± 0.034	1.045GlyPhe: 1.045 ± 0.023	1.5GlyGly: 1.5 ± 0.044	0.641GlyHis: 0.641 ± 0.013	2.377GlyIle: 2.377 ± 0.03	3.105GlyLys: 3.105 ± 0.051	2.017GlyLeu: 2.017 ± 0.045	0.599GlyMet: 0.599 ± 0.017	3.184GlyAsn: 3.184 ± 0.05	0.668GlyPro: 0.668 ± 0.03	0.701GlyGln: 0.701 ± 0.021	0.871GlyArg: 0.871 ± 0.017	2.001GlySer: 2.001 ± 0.05	1.495GlyThr: 1.495 ± 0.033	1.322GlyVal: 1.322 ± 0.02	0.161GlyTrp: 0.161 ± 0.007	1.441GlyTyr: 1.441 ± 0.026	0.0GlyXaa: 0.0 ± 0.0
His	0.482HisAla: 0.482 ± 0.018	0.316HisCys: 0.316 ± 0.018	1.42HisAsp: 1.42 ± 0.023	1.33HisGlu: 1.33 ± 0.023	1.286HisPhe: 1.286 ± 0.022	0.608HisGly: 0.608 ± 0.016	0.813HisHis: 0.813 ± 0.034	2.943HisIle: 2.943 ± 0.044	2.325HisLys: 2.325 ± 0.039	1.865HisLeu: 1.865 ± 0.026	0.873HisMet: 0.873 ± 0.019	3.52HisAsn: 3.52 ± 0.058	0.552HisPro: 0.552 ± 0.013	0.522HisGln: 0.522 ± 0.013	0.524HisArg: 0.524 ± 0.013	1.41HisSer: 1.41 ± 0.023	1.178HisThr: 1.178 ± 0.023	1.284HisVal: 1.284 ± 0.041	0.088HisTrp: 0.088 ± 0.005	1.083HisTyr: 1.083 ± 0.018	0.0HisXaa: 0.0 ± 0.0
Ile	1.481IleAla: 1.481 ± 0.027	2.059IleCys: 2.059 ± 0.029	4.809IleAsp: 4.809 ± 0.042	5.2IleGlu: 5.2 ± 0.062	4.65IlePhe: 4.65 ± 0.062	2.256IleGly: 2.256 ± 0.036	2.651IleHis: 2.651 ± 0.036	8.298IleIle: 8.298 ± 0.083	10.63IleLys: 10.63 ± 0.088	8.223IleLeu: 8.223 ± 0.073	1.651IleMet: 1.651 ± 0.023	12.962IleAsn: 12.962 ± 0.143	2.511IlePro: 2.511 ± 0.118	2.949IleGln: 2.949 ± 0.04	2.235IleArg: 2.235 ± 0.028	6.128IleSer: 6.128 ± 0.058	3.49IleThr: 3.49 ± 0.035	2.804IleVal: 2.804 ± 0.053	0.498IleTrp: 0.498 ± 0.013	6.438IleTyr: 6.438 ± 0.077	0.0IleXaa: 0.0 ± 0.0
Lys	1.805LysAla: 1.805 ± 0.03	2.099LysCys: 2.099 ± 0.038	7.019LysAsp: 7.019 ± 0.053	10.334LysGlu: 10.334 ± 0.099	3.118LysPhe: 3.118 ± 0.03	3.459LysGly: 3.459 ± 0.045	2.38LysHis: 2.38 ± 0.033	9.527LysIle: 9.527 ± 0.073	20.046LysLys: 20.046 ± 0.166	7.418LysLeu: 7.418 ± 0.061	2.544LysMet: 2.544 ± 0.032	17.119LysAsn: 17.119 ± 0.138	1.512LysPro: 1.512 ± 0.029	3.153LysGln: 3.153 ± 0.043	4.189LysArg: 4.189 ± 0.046	6.172LysSer: 6.172 ± 0.051	4.236LysThr: 4.236 ± 0.044	3.409LysVal: 3.409 ± 0.049	0.649LysTrp: 0.649 ± 0.02	7.01LysTyr: 7.01 ± 0.079	0.0LysXaa: 0.0 ± 0.0
Leu	1.523LeuAla: 1.523 ± 0.035	1.776LeuCys: 1.776 ± 0.026	3.543LeuAsp: 3.543 ± 0.033	4.403LeuGlu: 4.403 ± 0.06	4.355LeuPhe: 4.355 ± 0.051	1.99LeuGly: 1.99 ± 0.032	1.841LeuHis: 1.841 ± 0.027	6.165LeuIle: 6.165 ± 0.062	9.113LeuLys: 9.113 ± 0.081	7.391LeuLeu: 7.391 ± 0.084	1.272LeuMet: 1.272 ± 0.021	8.787LeuAsn: 8.787 ± 0.08	1.867LeuPro: 1.867 ± 0.036	2.361LeuGln: 2.361 ± 0.035	2.348LeuArg: 2.348 ± 0.032	5.538LeuSer: 5.538 ± 0.053	2.871LeuThr: 2.871 ± 0.038	2.418LeuVal: 2.418 ± 0.095	0.452LeuTrp: 0.452 ± 0.012	4.943LeuTyr: 4.943 ± 0.058	0.0LeuXaa: 0.0 ± 0.0
Met	0.387MetAla: 0.387 ± 0.011	0.458MetCys: 0.458 ± 0.012	1.607MetAsp: 1.607 ± 0.026	1.521MetGlu: 1.521 ± 0.03	0.857MetPhe: 0.857 ± 0.017	0.581MetGly: 0.581 ± 0.017	0.438MetHis: 0.438 ± 0.012	1.526MetIle: 1.526 ± 0.021	2.912MetLys: 2.912 ± 0.035	1.667MetLeu: 1.667 ± 0.025	0.475MetMet: 0.475 ± 0.014	4.01MetAsn: 4.01 ± 0.082	0.387MetPro: 0.387 ± 0.01	0.518MetGln: 0.518 ± 0.013	0.53MetArg: 0.53 ± 0.014	1.429MetSer: 1.429 ± 0.025	0.66MetThr: 0.66 ± 0.015	0.688MetVal: 0.688 ± 0.015	0.116MetTrp: 0.116 ± 0.006	1.197MetTyr: 1.197 ± 0.028	0.0MetXaa: 0.0 ± 0.0
Asn	1.908AsnAla: 1.908 ± 0.032	1.612AsnCys: 1.612 ± 0.029	10.256AsnAsp: 10.256 ± 0.109	9.655AsnGlu: 9.655 ± 0.093	4.746AsnPhe: 4.746 ± 0.052	3.167AsnGly: 3.167 ± 0.055	2.912AsnHis: 2.912 ± 0.054	16.433AsnIle: 16.433 ± 0.187	15.564AsnLys: 15.564 ± 0.13	7.603AsnLeu: 7.603 ± 0.07	4.114AsnMet: 4.114 ± 0.086	33.016AsnAsn: 33.016 ± 0.567	1.721AsnPro: 1.721 ± 0.033	2.857AsnGln: 2.857 ± 0.045	2.465AsnArg: 2.465 ± 0.034	7.646AsnSer: 7.646 ± 0.092	5.586AsnThr: 5.586 ± 0.063	6.359AsnVal: 6.359 ± 0.068	0.333AsnTrp: 0.333 ± 0.01	7.143AsnTyr: 7.143 ± 0.083	0.0AsnXaa: 0.0 ± 0.0
Pro	0.45ProAla: 0.45 ± 0.016	0.439ProCys: 0.439 ± 0.016	0.877ProAsp: 0.877 ± 0.031	1.31ProGlu: 1.31 ± 0.201	1.232ProPhe: 1.232 ± 0.022	0.554ProGly: 0.554 ± 0.016	0.554ProHis: 0.554 ± 0.013	1.699ProIle: 1.699 ± 0.023	1.74ProLys: 1.74 ± 0.033	1.843ProLeu: 1.843 ± 0.025	0.386ProMet: 0.386 ± 0.011	2.133ProAsn: 2.133 ± 0.038	0.799ProPro: 0.799 ± 0.031	0.698ProGln: 0.698 ± 0.02	0.539ProArg: 0.539 ± 0.017	1.643ProSer: 1.643 ± 0.026	1.032ProThr: 1.032 ± 0.024	0.765ProVal: 0.765 ± 0.02	0.132ProTrp: 0.132 ± 0.006	1.316ProTyr: 1.316 ± 0.024	0.0ProXaa: 0.0 ± 0.0
Gln	0.582GlnAla: 0.582 ± 0.016	0.412GlnCys: 0.412 ± 0.011	1.395GlnAsp: 1.395 ± 0.024	2.032GlnGlu: 2.032 ± 0.043	0.968GlnPhe: 0.968 ± 0.019	0.76GlnGly: 0.76 ± 0.018	0.747GlnHis: 0.747 ± 0.02	2.45GlnIle: 2.45 ± 0.031	3.575GlnLys: 3.575 ± 0.039	1.911GlnLeu: 1.911 ± 0.028	0.704GlnMet: 0.704 ± 0.018	4.305GlnAsn: 4.305 ± 0.067	0.551GlnPro: 0.551 ± 0.017	1.143GlnGln: 1.143 ± 0.042	0.818GlnArg: 0.818 ± 0.016	1.468GlnSer: 1.468 ± 0.029	1.309GlnThr: 1.309 ± 0.024	0.954GlnVal: 0.954 ± 0.016	0.164GlnTrp: 0.164 ± 0.008	1.369GlnTyr: 1.369 ± 0.022	0.0GlnXaa: 0.0 ± 0.0
Arg	0.631ArgAla: 0.631 ± 0.016	0.441ArgCys: 0.441 ± 0.013	1.526ArgAsp: 1.526 ± 0.034	1.763ArgGlu: 1.763 ± 0.035	0.964ArgPhe: 0.964 ± 0.017	0.955ArgGly: 0.955 ± 0.022	0.564ArgHis: 0.564 ± 0.013	2.116ArgIle: 2.116 ± 0.028	3.863ArgLys: 3.863 ± 0.039	1.784ArgLeu: 1.784 ± 0.04	0.53ArgMet: 0.53 ± 0.014	3.415ArgAsn: 3.415 ± 0.044	0.474ArgPro: 0.474 ± 0.016	0.708ArgGln: 0.708 ± 0.015	1.423ArgArg: 1.423 ± 0.034	1.594ArgSer: 1.594 ± 0.031	1.129ArgThr: 1.129 ± 0.022	0.865ArgVal: 0.865 ± 0.018	0.205ArgTrp: 0.205 ± 0.01	1.325ArgTyr: 1.325 ± 0.023	0.0ArgXaa: 0.0 ± 0.0
Ser	1.364SerAla: 1.364 ± 0.024	1.225SerCys: 1.225 ± 0.02	4.143SerAsp: 4.143 ± 0.056	3.471SerGlu: 3.471 ± 0.041	3.437SerPhe: 3.437 ± 0.04	2.09SerGly: 2.09 ± 0.054	1.602SerHis: 1.602 ± 0.037	5.428SerIle: 5.428 ± 0.05	5.653SerLys: 5.653 ± 0.05	5.238SerLeu: 5.238 ± 0.048	1.179SerMet: 1.179 ± 0.022	8.244SerAsn: 8.244 ± 0.1	1.41SerPro: 1.41 ± 0.03	1.647SerGln: 1.647 ± 0.027	1.563SerArg: 1.563 ± 0.027	6.567SerSer: 6.567 ± 0.081	3.093SerThr: 3.093 ± 0.039	2.624SerVal: 2.624 ± 0.051	0.278SerTrp: 0.278 ± 0.009	3.779SerTyr: 3.779 ± 0.043	0.0SerXaa: 0.0 ± 0.0
Thr	0.926ThrAla: 0.926 ± 0.022	1.005ThrCys: 1.005 ± 0.021	2.055ThrAsp: 2.055 ± 0.027	2.114ThrGlu: 2.114 ± 0.057	2.16ThrPhe: 2.16 ± 0.03	1.121ThrGly: 1.121 ± 0.028	1.226ThrHis: 1.226 ± 0.026	3.08ThrIle: 3.08 ± 0.033	4.058ThrLys: 4.058 ± 0.039	3.38ThrLeu: 3.38 ± 0.037	0.721ThrMet: 0.721 ± 0.022	5.926ThrAsn: 5.926 ± 0.07	1.16ThrPro: 1.16 ± 0.025	1.392ThrGln: 1.392 ± 0.028	0.924ThrArg: 0.924 ± 0.019	3.246ThrSer: 3.246 ± 0.034	2.282ThrThr: 2.282 ± 0.042	1.321ThrVal: 1.321 ± 0.023	0.211ThrTrp: 0.211 ± 0.008	2.791ThrTyr: 2.791 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	0.985ValAla: 0.985 ± 0.029	0.82ValCys: 0.82 ± 0.019	2.709ValAsp: 2.709 ± 0.037	2.969ValGlu: 2.969 ± 0.197	1.52ValPhe: 1.52 ± 0.024	1.398ValGly: 1.398 ± 0.034	1.224ValHis: 1.224 ± 0.021	3.091ValIle: 3.091 ± 0.113	3.582ValLys: 3.582 ± 0.04	3.302ValLeu: 3.302 ± 0.044	0.685ValMet: 0.685 ± 0.016	4.061ValAsn: 4.061 ± 0.057	1.215ValPro: 1.215 ± 0.094	1.357ValGln: 1.357 ± 0.027	0.984ValArg: 0.984 ± 0.019	2.868ValSer: 2.868 ± 0.093	1.617ValThr: 1.617 ± 0.037	1.869ValVal: 1.869 ± 0.147	0.234ValTrp: 0.234 ± 0.009	1.943ValTyr: 1.943 ± 0.027	0.0ValXaa: 0.0 ± 0.0
Trp	0.154TrpAla: 0.154 ± 0.008	0.093TrpCys: 0.093 ± 0.005	0.285TrpAsp: 0.285 ± 0.01	0.319TrpGlu: 0.319 ± 0.013	0.251TrpPhe: 0.251 ± 0.01	0.212TrpGly: 0.212 ± 0.009	0.073TrpHis: 0.073 ± 0.005	0.475TrpIle: 0.475 ± 0.014	0.644TrpLys: 0.644 ± 0.018	0.424TrpLeu: 0.424 ± 0.012	0.102TrpMet: 0.102 ± 0.006	0.537TrpAsn: 0.537 ± 0.012	0.094TrpPro: 0.094 ± 0.005	0.09TrpGln: 0.09 ± 0.005	0.171TrpArg: 0.171 ± 0.007	0.305TrpSer: 0.305 ± 0.01	0.191TrpThr: 0.191 ± 0.009	0.226TrpVal: 0.226 ± 0.009	0.088TrpTrp: 0.088 ± 0.007	0.212TrpTyr: 0.212 ± 0.008	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.1TyrAla: 1.1 ± 0.018	0.843TyrCys: 0.843 ± 0.019	4.466TyrAsp: 4.466 ± 0.055	3.897TyrGlu: 3.897 ± 0.037	2.925TyrPhe: 2.925 ± 0.039	1.498TyrGly: 1.498 ± 0.028	1.313TyrHis: 1.313 ± 0.025	6.038TyrIle: 6.038 ± 0.081	5.652TyrLys: 5.652 ± 0.057	4.31TyrLeu: 4.31 ± 0.045	1.38TyrMet: 1.38 ± 0.024	7.848TyrAsn: 7.848 ± 0.094	1.133TyrPro: 1.133 ± 0.022	1.18TyrGln: 1.18 ± 0.02	1.258TyrArg: 1.258 ± 0.02	3.474TyrSer: 3.474 ± 0.04	2.286TyrThr: 2.286 ± 0.031	2.425TyrVal: 2.425 ± 0.028	0.215TyrTrp: 0.215 ± 0.008	3.455TyrTyr: 3.455 ± 0.05	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5057 proteins (3756975 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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