Rhodobacter sp. LPB0142

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Rhodobacter; unclassified Rhodobacter

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3414 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D9M908|A0A1D9M908_9RHOB FAD-dependent oxidoreductase OS=Rhodobacter sp. LPB0142 OX=1850250 GN=LPB142_02640 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 10.19KK3 pKa = 10.41VLFATTALVMTAGVAAAEE21 pKa = 4.39VSLSGDD27 pKa = 2.68GRR29 pKa = 11.84MGIVYY34 pKa = 10.52NGADD38 pKa = 3.18WNFSSRR44 pKa = 11.84ARR46 pKa = 11.84VTFTLSGTTDD56 pKa = 2.92NGIEE60 pKa = 4.0FGGSFRR66 pKa = 11.84ADD68 pKa = 3.17QAGDD72 pKa = 3.68SVLDD76 pKa = 3.45NGAAYY81 pKa = 9.93GSEE84 pKa = 3.78GSVYY88 pKa = 10.63VSGAFGKK95 pKa = 9.98IEE97 pKa = 3.97MGDD100 pKa = 3.28AVSAPEE106 pKa = 4.53ALFGDD111 pKa = 4.52LPEE114 pKa = 4.66VGYY117 pKa = 9.44TDD119 pKa = 3.69LTSAGGLEE127 pKa = 3.91NDD129 pKa = 3.53IPYY132 pKa = 9.23LTGDD136 pKa = 3.75GVTTLAGNPVVLYY149 pKa = 9.13TYY151 pKa = 9.17STGPISIAASMSDD164 pKa = 3.01GKK166 pKa = 10.84LGSVNDD172 pKa = 3.64QQEE175 pKa = 4.24YY176 pKa = 10.52AVAGAYY182 pKa = 8.84TYY184 pKa = 11.3EE185 pKa = 4.23NYY187 pKa = 9.67TVGFGYY193 pKa = 10.99EE194 pKa = 3.89MLEE197 pKa = 4.29DD198 pKa = 3.64VGGVAGVDD206 pKa = 3.57YY207 pKa = 9.44TQGEE211 pKa = 4.45LAGVATFGDD220 pKa = 4.17TSVKK224 pKa = 10.54AYY226 pKa = 8.62FAKK229 pKa = 10.48GGKK232 pKa = 8.48QNPVDD237 pKa = 3.47QAFGLGVTSKK247 pKa = 10.64FGATTVLGYY256 pKa = 8.17VQKK259 pKa = 11.4AEE261 pKa = 4.24FTAGGDD267 pKa = 3.71VTWYY271 pKa = 10.91GLGASYY277 pKa = 11.27DD278 pKa = 3.84LGGGASIVGGISDD291 pKa = 5.64DD292 pKa = 4.37DD293 pKa = 4.44LANTDD298 pKa = 3.41PTADD302 pKa = 3.76LGVKK306 pKa = 10.34FKK308 pKa = 11.05FF309 pKa = 3.81

Molecular weight: 31.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D9M963|A0A1D9M963_9RHOB RNA pseudouridine synthase OS=Rhodobacter sp. LPB0142 OX=1850250 GN=LPB142_02200 PE=4 SV=1
MM1 pKa = 7.86DD2 pKa = 4.61FKK4 pKa = 11.26AIAMGLAFALMWSSAFTSARR24 pKa = 11.84MIVADD29 pKa = 4.87APPMAALALRR39 pKa = 11.84FLVSGLIGVGIALAVGQSWRR59 pKa = 11.84LSRR62 pKa = 11.84AQWRR66 pKa = 11.84ATLIFGLCQNALYY79 pKa = 10.72LGLNFVAMQWVQASVASIIASTMPLMVAGLGWAVFGDD116 pKa = 3.98RR117 pKa = 11.84VRR119 pKa = 11.84PLGVAGLGAGVVGVAIIMGARR140 pKa = 11.84LSGGVDD146 pKa = 3.37PVGMALCFAGALALSIATLSVRR168 pKa = 11.84GAASGGNVMMIVGLQMFVGAAILASASAAIEE199 pKa = 4.07TWEE202 pKa = 4.35VTWSARR208 pKa = 11.84LVWAFLYY215 pKa = 7.48TTLIPGVLATWVWFKK230 pKa = 11.21LVGRR234 pKa = 11.84IGAVKK239 pKa = 10.04AATFHH244 pKa = 6.31FLNPFFGVAIAALVLGEE261 pKa = 4.42RR262 pKa = 11.84LGALDD267 pKa = 4.21LLGVAVIAGGILAVQLSKK285 pKa = 10.9QAA287 pKa = 3.49

Molecular weight: 29.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3414

0

3414

1065451

38

2468

312.1

33.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.408 ± 0.076

0.834 ± 0.013

5.185 ± 0.04

6.335 ± 0.037

3.586 ± 0.025

9.062 ± 0.049

1.892 ± 0.021

4.825 ± 0.031

3.006 ± 0.035

10.374 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.585 ± 0.02

2.167 ± 0.022

5.42 ± 0.038

2.855 ± 0.023

7.25 ± 0.048

4.583 ± 0.028

5.068 ± 0.031

7.046 ± 0.035

1.434 ± 0.02

2.085 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski